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The map label for this gene is metE

Identifier: 92112804

GI number: 92112804

Start: 753996

End: 756281

Strand: Direct

Name: metE

Synonym: Csal_0672

Alternate gene names: 92112804

Gene position: 753996-756281 (Clockwise)

Preceding gene: 92112802

Following gene: 92112805

Centisome position: 20.4

GC content: 66.19

Gene sequence:

>2286_bases
ATGGCGCTGGCACACATTCTTGGCTATCCGCGCATTGGCGCGAATCGCGAACTCAAAAAGGCCGTCGAGGCTTACTGGAA
GGGAGATATCGACCAGGCCGAGCTGGAACGTCGTGGCCAGGCGTTGCGGGCCGAGCACTGGCAGGCCAGCCGCGATGCGG
GACTCGATTTCGTGACCGTCGGCGATTTCGCCTTCTACGACCAGGTGCTGAACGTCAGCGCGATGCTCGGCGCGGTGCCG
CCGCGCTTCGGCGCCATCGACGGCGATGTCGACCTGGATACGACATTTCGCATGGCGCGTGGCCGCGCGCCGAGCGGCAC
CCCCGCCGCCGCCTGCGAAATGACCAAGTACTTCGATACCAATTATCACTACCTGGTGCCCGAGCTGCATGCCGGCCAGC
GCTTCCGGGTGGCGTCCACGCGCTTGTTCGACGAGGTCGCCGAGGCCCAGGCCGCGGGGCATCCGGTCAAGGTGGCCCTG
CTGGGGCCGGTGAGCTGGTTGTGGCTGGGCAAGGAAAAGAGTGCCGGGCTCGACCGCCTGACGCTGCTCGACGACGTGTT
GTCGGTCTATGGGGAAATCCTCGAGCGTCTCGCCGCGCAAGGTGTCGAATGGGTGCAGCTCGACGAGCCGGCGCTGGTGC
AGGATCTGCCCCGCGATTGGCGCCAGGCGTTCGAGTCCGCCTACAACAAGCTGCAATCCGCGCCGGTCAAGCTGTTGCTG
GCGACCTACTTCGGGGCGCTGGGCGACAACCTGGGCCTTGCCGCAGGGCTGCCGGTAGCCGGCCTGCATATCGACACGGT
GCGGGCGCCCGAGCAACTCGACGCCGTGCTCGATCGCCTGCCCACCTACAAGGTGCTGTCCCTTGGCGCCATCGACGGGC
GCAACATCTGGCGCGCCGATCTGGCAGCGCTGCGTGAGCGTCTGCAGGTAGCCCGGGCGCGTCTCGGCGAGCGCCTGTGG
ATCTCCGCCTCCTGCTCGTTGCTGCATGTGCCGGTGGACCTCGACGCGGAAACCGACCTCGATGCCGAGCTCAAGAGCTG
GCTGGCCTTCGCGCGGCAAAAGCTCGACGAGATCGTCACGCTGGCGCATCTGCTCGACGGTCGCGCCACGCCGGAAGACA
CCGCGCGACTGGAAGCTGCGACGTCGGCGCTGGACGCACGCCGTCAATCGCCGCGCATCCACAAGCCGGCCGTGGGCGAG
CGTCTGGCGGCGGTGTCCGCCGACGATGCCGAGCGTGCAAGCCCCTACGCGACGCGTGCCAAGGCGCAGCATCGCCACCT
GGACCTGCCACTGTTTCCGACCACCACGATCGGCTCCTTTCCGCAGACCCAGGACATCCGGGCCGCACGACGCGCCTTCA
AGAGCGGCGAGCTGAGCCGCGATGACTACGAGGCCCGCATGCGCGACGAAATCGCCCATGCCGTCGAACGCCAGGAAGCG
CTGGCCATCGACGTGCCCGTGCACGGCGAGCCGGAACGTAACGATATGGTCGAGTACTTCGGCGAGCTGCTGGACGGCTT
TGCCTTCACGCGTTTCGGCTGGGTGCAGAGTTACGGCTCGCGCTGCGTGAAGCCGCCGGTGATCTTCGGCGATGTGAGCC
GTCCCGGGCCGATGACGGTGCGCTGGAGCGAATACGCGCAATCGCTGACCGACAAGCCGATGAAGGGCATGCTCACCGGC
CCGGTGACCATCCTGCAGTGGTCCTTCGTCCGCGATGACCAGCCGCGCGATGCCACCTGTCGGCAGATTGCCCTGTCCCT
CCGGGACGAGGTCGCCGATCTCGAGGCGGCAGGCATCAAGATCATCCAGATCGACGAGCCGGCACTGCGTGAAGGCCTGC
CGCTGCGGCAGGGTGAGTGGCAGGAGTATCTCGACTGGGCAGTGAAAAGCTTCAAGCTGAGCGCGGCGGTGGCCCGTGAC
GAGACGCAGATCCACACGCACATGTGCTACTCGGAATTCAACGACATCATCGCGGCGATCGCGGCCCTGGATGCCGACGT
CATCACCATCGAGACGTCGCGTTCCGACATGGAACTGCTCGATGCCTTTCAGGATTTCGCGTATCCCAACGAGATCGGCC
CGGGGGTGTATGACATCCATTCGCCGAACATTCCCGAGGTGGAATGGATGGTGAGCCTGATGGAAAAGGCCGCCGAGAAA
ATCCCGGCCGAGCGGCTTTGGGTGAACCCGGACTGTGGCCTGAAAACACGCGGGTGGGCCGAAGTCGAGCCGGCGCTGGC
CAACATGGTGGAGGCCGCACGGGAGTTGCGTCGCCGTTACGGCTGA

Upstream 100 bases:

>100_bases
ACTGCTGCCAGCGCGTCACGGCACCGCGACGCCGTGATACAGAAAGTCACTTATTGCTGAAAGTCACTCATTCTAGCCGG
ATACCGCAAGGGGAAACACG

Downstream 100 bases:

>100_bases
GGTCGTCCGACGACGTCCTGCACGCGCCGCCGGGGCATCCCCCGGCGGCGTTTGCGTTTCTTGCGGCAGTGACGTTTCTC
ACGGCGAACGGGCTGTTATG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MALAHILGYPRIGANRELKKAVEAYWKGDIDQAELERRGQALRAEHWQASRDAGLDFVTVGDFAFYDQVLNVSAMLGAVP
PRFGAIDGDVDLDTTFRMARGRAPSGTPAAACEMTKYFDTNYHYLVPELHAGQRFRVASTRLFDEVAEAQAAGHPVKVAL
LGPVSWLWLGKEKSAGLDRLTLLDDVLSVYGEILERLAAQGVEWVQLDEPALVQDLPRDWRQAFESAYNKLQSAPVKLLL
ATYFGALGDNLGLAAGLPVAGLHIDTVRAPEQLDAVLDRLPTYKVLSLGAIDGRNIWRADLAALRERLQVARARLGERLW
ISASCSLLHVPVDLDAETDLDAELKSWLAFARQKLDEIVTLAHLLDGRATPEDTARLEAATSALDARRQSPRIHKPAVGE
RLAAVSADDAERASPYATRAKAQHRHLDLPLFPTTTIGSFPQTQDIRAARRAFKSGELSRDDYEARMRDEIAHAVERQEA
LAIDVPVHGEPERNDMVEYFGELLDGFAFTRFGWVQSYGSRCVKPPVIFGDVSRPGPMTVRWSEYAQSLTDKPMKGMLTG
PVTILQWSFVRDDQPRDATCRQIALSLRDEVADLEAAGIKIIQIDEPALREGLPLRQGEWQEYLDWAVKSFKLSAAVARD
ETQIHTHMCYSEFNDIIAAIAALDADVITIETSRSDMELLDAFQDFAYPNEIGPGVYDIHSPNIPEVEWMVSLMEKAAEK
IPAERLWVNPDCGLKTRGWAEVEPALANMVEAARELRRRYG

Sequences:

>Translated_761_residues
MALAHILGYPRIGANRELKKAVEAYWKGDIDQAELERRGQALRAEHWQASRDAGLDFVTVGDFAFYDQVLNVSAMLGAVP
PRFGAIDGDVDLDTTFRMARGRAPSGTPAAACEMTKYFDTNYHYLVPELHAGQRFRVASTRLFDEVAEAQAAGHPVKVAL
LGPVSWLWLGKEKSAGLDRLTLLDDVLSVYGEILERLAAQGVEWVQLDEPALVQDLPRDWRQAFESAYNKLQSAPVKLLL
ATYFGALGDNLGLAAGLPVAGLHIDTVRAPEQLDAVLDRLPTYKVLSLGAIDGRNIWRADLAALRERLQVARARLGERLW
ISASCSLLHVPVDLDAETDLDAELKSWLAFARQKLDEIVTLAHLLDGRATPEDTARLEAATSALDARRQSPRIHKPAVGE
RLAAVSADDAERASPYATRAKAQHRHLDLPLFPTTTIGSFPQTQDIRAARRAFKSGELSRDDYEARMRDEIAHAVERQEA
LAIDVPVHGEPERNDMVEYFGELLDGFAFTRFGWVQSYGSRCVKPPVIFGDVSRPGPMTVRWSEYAQSLTDKPMKGMLTG
PVTILQWSFVRDDQPRDATCRQIALSLRDEVADLEAAGIKIIQIDEPALREGLPLRQGEWQEYLDWAVKSFKLSAAVARD
ETQIHTHMCYSEFNDIIAAIAALDADVITIETSRSDMELLDAFQDFAYPNEIGPGVYDIHSPNIPEVEWMVSLMEKAAEK
IPAERLWVNPDCGLKTRGWAEVEPALANMVEAARELRRRYG
>Mature_760_residues
ALAHILGYPRIGANRELKKAVEAYWKGDIDQAELERRGQALRAEHWQASRDAGLDFVTVGDFAFYDQVLNVSAMLGAVPP
RFGAIDGDVDLDTTFRMARGRAPSGTPAAACEMTKYFDTNYHYLVPELHAGQRFRVASTRLFDEVAEAQAAGHPVKVALL
GPVSWLWLGKEKSAGLDRLTLLDDVLSVYGEILERLAAQGVEWVQLDEPALVQDLPRDWRQAFESAYNKLQSAPVKLLLA
TYFGALGDNLGLAAGLPVAGLHIDTVRAPEQLDAVLDRLPTYKVLSLGAIDGRNIWRADLAALRERLQVARARLGERLWI
SASCSLLHVPVDLDAETDLDAELKSWLAFARQKLDEIVTLAHLLDGRATPEDTARLEAATSALDARRQSPRIHKPAVGER
LAAVSADDAERASPYATRAKAQHRHLDLPLFPTTTIGSFPQTQDIRAARRAFKSGELSRDDYEARMRDEIAHAVERQEAL
AIDVPVHGEPERNDMVEYFGELLDGFAFTRFGWVQSYGSRCVKPPVIFGDVSRPGPMTVRWSEYAQSLTDKPMKGMLTGP
VTILQWSFVRDDQPRDATCRQIALSLRDEVADLEAAGIKIIQIDEPALREGLPLRQGEWQEYLDWAVKSFKLSAAVARDE
TQIHTHMCYSEFNDIIAAIAALDADVITIETSRSDMELLDAFQDFAYPNEIGPGVYDIHSPNIPEVEWMVSLMEKAAEKI
PAERLWVNPDCGLKTRGWAEVEPALANMVEAARELRRRYG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=60.2377807133421, Blast_Score=935, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=773, Percent_Identity=47.6067270375162, Blast_Score=714, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_CHRSD (Q1QZS5)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_572732.1
- ProteinModelPortal:   Q1QZS5
- SMR:   Q1QZS5
- STRING:   Q1QZS5
- GeneID:   4026700
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_0672
- NMPDR:   fig|290398.4.peg.569
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   CSAL290398:CSAL_0672-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 84664; Mature: 84533

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAHILGYPRIGANRELKKAVEAYWKGDIDQAELERRGQALRAEHWQASRDAGLDFVTV
CCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCEEEE
GDFAFYDQVLNVSAMLGAVPPRFGAIDGDVDLDTTFRMARGRAPSGTPAAACEMTKYFDT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCC
NYHYLVPELHAGQRFRVASTRLFDEVAEAQAAGHPVKVALLGPVSWLWLGKEKSAGLDRL
CCEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCHHH
TLLDDVLSVYGEILERLAAQGVEWVQLDEPALVQDLPRDWRQAFESAYNKLQSAPVKLLL
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ATYFGALGDNLGLAAGLPVAGLHIDTVRAPEQLDAVLDRLPTYKVLSLGAIDGRNIWRAD
HHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
LAALRERLQVARARLGERLWISASCSLLHVPVDLDAETDLDAELKSWLAFARQKLDEIVT
HHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAHLLDGRATPEDTARLEAATSALDARRQSPRIHKPAVGERLAAVSADDAERASPYATRA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHCCCHHHHH
KAQHRHLDLPLFPTTTIGSFPQTQDIRAARRAFKSGELSRDDYEARMRDEIAHAVERQEA
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
LAIDVPVHGEPERNDMVEYFGELLDGFAFTRFGWVQSYGSRCVKPPVIFGDVSRPGPMTV
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEE
RWSEYAQSLTDKPMKGMLTGPVTILQWSFVRDDQPRDATCRQIALSLRDEVADLEAAGIK
EHHHHHHHHCCCCCCCCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEE
IIQIDEPALREGLPLRQGEWQEYLDWAVKSFKLSAAVARDETQIHTHMCYSEFNDIIAAI
EEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AALDADVITIETSRSDMELLDAFQDFAYPNEIGPGVYDIHSPNIPEVEWMVSLMEKAAEK
HHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
IPAERLWVNPDCGLKTRGWAEVEPALANMVEAARELRRRYG
CCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALAHILGYPRIGANRELKKAVEAYWKGDIDQAELERRGQALRAEHWQASRDAGLDFVTV
CHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCEEEE
GDFAFYDQVLNVSAMLGAVPPRFGAIDGDVDLDTTFRMARGRAPSGTPAAACEMTKYFDT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCC
NYHYLVPELHAGQRFRVASTRLFDEVAEAQAAGHPVKVALLGPVSWLWLGKEKSAGLDRL
CCEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCHHH
TLLDDVLSVYGEILERLAAQGVEWVQLDEPALVQDLPRDWRQAFESAYNKLQSAPVKLLL
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ATYFGALGDNLGLAAGLPVAGLHIDTVRAPEQLDAVLDRLPTYKVLSLGAIDGRNIWRAD
HHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
LAALRERLQVARARLGERLWISASCSLLHVPVDLDAETDLDAELKSWLAFARQKLDEIVT
HHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAHLLDGRATPEDTARLEAATSALDARRQSPRIHKPAVGERLAAVSADDAERASPYATRA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHCCCHHHHH
KAQHRHLDLPLFPTTTIGSFPQTQDIRAARRAFKSGELSRDDYEARMRDEIAHAVERQEA
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
LAIDVPVHGEPERNDMVEYFGELLDGFAFTRFGWVQSYGSRCVKPPVIFGDVSRPGPMTV
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEE
RWSEYAQSLTDKPMKGMLTGPVTILQWSFVRDDQPRDATCRQIALSLRDEVADLEAAGIK
EHHHHHHHHCCCCCCCCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEE
IIQIDEPALREGLPLRQGEWQEYLDWAVKSFKLSAAVARDETQIHTHMCYSEFNDIIAAI
EEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AALDADVITIETSRSDMELLDAFQDFAYPNEIGPGVYDIHSPNIPEVEWMVSLMEKAAEK
HHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
IPAERLWVNPDCGLKTRGWAEVEPALANMVEAARELRRRYG
CCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA