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The map label for this gene is folM [H]

Identifier: 92112720

GI number: 92112720

Start: 652496

End: 653209

Strand: Direct

Name: folM [H]

Synonym: Csal_0587

Alternate gene names: 92112720

Gene position: 652496-653209 (Clockwise)

Preceding gene: 92112719

Following gene: 92112721

Centisome position: 17.65

GC content: 64.85

Gene sequence:

>714_bases
ATGAGCCAGTCGCCGATTCTCGTGACCGGGGGCGCCCAGCGCCTCGGGCGTCATTGCGTGGAGCGTCTGCTCGACGATGG
CCAGCCGGTGATATTGAGCTACCGGCGCGAGCGCGAGGCACTGGACGCCTTGCGCCAGCGTGGTGCCGTGACCCTGCAGG
CGGATTTCTCGAGCGAAGCAGGCATCCTCGACTTCATCGCCCGCCTGAAGGCACAGACGTCGTCGCTGCGCGCCATCGTC
CACAACGCCAGCGACTGGGCGCCGGACAGTCTCGGCGATGACGCCGGCGCCAATTTCGAACGGTTGTTCCGCGTGCACAT
GCAGGCGCCGTACCTGATCAACCTGCATGCTCGTGAATTGCTCGACGCCTGCCAGGAGCCCCAGCGCGACATCATCCACA
TGACCGATTACGTCAGTCAGAAGGGGTCGCGCAAGCATGCCGCCTACGCGGCGACCAAGGCGGGACTGGACAATCTGACC
CTGTCCTTCGCGGCGATGTACGCGCCGAGCATCCAGGTCAATGCCATCGCGCCAGCCCTGATCATGCTCAACGAAGGTGA
CGACGAGGCCTATGCCGAGAAGGCCAAGGCCAAGTCGCTGATGGCCAAGGTGCCGGGGCCGGGCGTGATCTATCAGACGC
TGCGTTATCTGCTCGACAACGATTTCGTGACCGGTGCGATCCTGCCCGTGGACGGCGGGCGGCACTTGCGCTGA

Upstream 100 bases:

>100_bases
TCTTCGCCTATGCGCCGCGCGTACCGTTGATCCCCCTGCCGGAAGAGGTTAGCGAAAAAGCCCCGCTGTCACCCGGGGAG
CGTCGTGCGGAGGATGATGC

Downstream 100 bases:

>100_bases
GGGGCATCGGCCGGCGTGCACTTGTCAAATGACGACAGCCGTGTAGGATGAGGCCATGTTCTGGAGTCAACCCATGCCAA
TAGCATCATTCACCTCGAAC

Product: short chain dehydrogenase

Products: NA

Alternate protein names: DHFR [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSQSPILVTGGAQRLGRHCVERLLDDGQPVILSYRREREALDALRQRGAVTLQADFSSEAGILDFIARLKAQTSSLRAIV
HNASDWAPDSLGDDAGANFERLFRVHMQAPYLINLHARELLDACQEPQRDIIHMTDYVSQKGSRKHAAYAATKAGLDNLT
LSFAAMYAPSIQVNAIAPALIMLNEGDDEAYAEKAKAKSLMAKVPGPGVIYQTLRYLLDNDFVTGAILPVDGGRHLR

Sequences:

>Translated_237_residues
MSQSPILVTGGAQRLGRHCVERLLDDGQPVILSYRREREALDALRQRGAVTLQADFSSEAGILDFIARLKAQTSSLRAIV
HNASDWAPDSLGDDAGANFERLFRVHMQAPYLINLHARELLDACQEPQRDIIHMTDYVSQKGSRKHAAYAATKAGLDNLT
LSFAAMYAPSIQVNAIAPALIMLNEGDDEAYAEKAKAKSLMAKVPGPGVIYQTLRYLLDNDFVTGAILPVDGGRHLR
>Mature_236_residues
SQSPILVTGGAQRLGRHCVERLLDDGQPVILSYRREREALDALRQRGAVTLQADFSSEAGILDFIARLKAQTSSLRAIVH
NASDWAPDSLGDDAGANFERLFRVHMQAPYLINLHARELLDACQEPQRDIIHMTDYVSQKGSRKHAAYAATKAGLDNLTL
SFAAMYAPSIQVNAIAPALIMLNEGDDEAYAEKAKAKSLMAKVPGPGVIYQTLRYLLDNDFVTGAILPVDGGRHLR

Specific function: Catalyzes the reduction of dihydrofolate to tetrahydrofolate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787891, Length=233, Percent_Identity=45.4935622317597, Blast_Score=206, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI115534694, Length=242, Percent_Identity=26.8595041322314, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571526, Length=248, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24643142, Length=203, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 25969; Mature: 25838

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSPILVTGGAQRLGRHCVERLLDDGQPVILSYRREREALDALRQRGAVTLQADFSSEA
CCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCEEEEECCCCCC
GILDFIARLKAQTSSLRAIVHNASDWAPDSLGDDAGANFERLFRVHMQAPYLINLHAREL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEHHHHHH
LDACQEPQRDIIHMTDYVSQKGSRKHAAYAATKAGLDNLTLSFAAMYAPSIQVNAIAPAL
HHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEE
IMLNEGDDEAYAEKAKAKSLMAKVPGPGVIYQTLRYLLDNDFVTGAILPVDGGRHLR
EEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SQSPILVTGGAQRLGRHCVERLLDDGQPVILSYRREREALDALRQRGAVTLQADFSSEA
CCCCEEEECCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCEEEEECCCCCC
GILDFIARLKAQTSSLRAIVHNASDWAPDSLGDDAGANFERLFRVHMQAPYLINLHAREL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEHHHHHH
LDACQEPQRDIIHMTDYVSQKGSRKHAAYAATKAGLDNLTLSFAAMYAPSIQVNAIAPAL
HHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEE
IMLNEGDDEAYAEKAKAKSLMAKVPGPGVIYQTLRYLLDNDFVTGAILPVDGGRHLR
EEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA