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The map label for this gene is fre [H]

Identifier: 92112719

GI number: 92112719

Start: 651687

End: 652499

Strand: Direct

Name: fre [H]

Synonym: Csal_0586

Alternate gene names: 92112719

Gene position: 651687-652499 (Clockwise)

Preceding gene: 92112718

Following gene: 92112720

Centisome position: 17.63

GC content: 65.93

Gene sequence:

>813_bases
ATGACGTCTAAGACACTGACCTGCCAAGTCACGTCGGTCGAGGATCTGACGCCCGACGTATTCCGCGTCCATTTGACGGG
ACGCCGCGAAGCCATTGCGCATGCGCCGGGGCAGTACCTGGAACTCAAGCTTGATGAGCAAACCTGGGTACCGTTTTCCA
TCGCCAACGCGCATGCCGATGACGGGGTCATCGAGCTGCACGTGCAGCACTGGCCGGAGCGCAGCAATTCGGCCCGCCTG
CGCGAATTGCTGGTGCACGCCGCGCGACTGACGGTGCGTCTGCCCAATGGTGGCTGCGTGCTCGATACCCGCAGTCGGCG
TCCCTTGACGCTGATCGCCGCCGGGACGGGATTCGCCCAGATGAAGGCGATCGTCGAGGCGGCGCTGGCCAACGATCACC
CGGGGCCGATTCGGCTGTGGTGGGCAGTCAAGTCGCCCCGTGACCTGTACCTGGAAAGTCTGGCGCTCGAATGGGCCGCC
ACGCATCCCCACCTCGACGTGCATACCGTGGTCGAGGACAGCGAGCCCGCGCTGAGCGCGCCGGCCGGCGTGACGCGGCA
CATCGGCCGCATCGACGAGGTGCTTGCCGAGTGCCACGGCGACATCTCCGGCGACGATGTCTACCTGTCCGGCTCGCCGG
GCATGGTGTACGCCTGCATCGACACTCTGGCCCCGCTGGGGCTCGACCCCGAGCGAGTGTTTTCCGATGTCTTCGCCTAT
GCGCCGCGCGTACCGTTGATCCCCCTGCCGGAAGAGGTTAGCGAAAAAGCCCCGCTGTCACCCGGGGAGCGTCGTGCGGA
GGATGATGCATGA

Upstream 100 bases:

>100_bases
GCCGTCAAGCGGCGCGTCGATGAGCGCTGGGGGGAATACGGCATCGCCACGCCGCCACCGCCACCCCGCCATTCGCCCCC
GAGCGACGAACGAGGACACG

Downstream 100 bases:

>100_bases
GCCAGTCGCCGATTCTCGTGACCGGGGGCGCCCAGCGCCTCGGGCGTCATTGCGTGGAGCGTCTGCTCGACGATGGCCAG
CCGGTGATATTGAGCTACCG

Product: oxidoreductase FAD/NAD(P)-binding

Products: NA

Alternate protein names: Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase C; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTSKTLTCQVTSVEDLTPDVFRVHLTGRREAIAHAPGQYLELKLDEQTWVPFSIANAHADDGVIELHVQHWPERSNSARL
RELLVHAARLTVRLPNGGCVLDTRSRRPLTLIAAGTGFAQMKAIVEAALANDHPGPIRLWWAVKSPRDLYLESLALEWAA
THPHLDVHTVVEDSEPALSAPAGVTRHIGRIDEVLAECHGDISGDDVYLSGSPGMVYACIDTLAPLGLDPERVFSDVFAY
APRVPLIPLPEEVSEKAPLSPGERRAEDDA

Sequences:

>Translated_270_residues
MTSKTLTCQVTSVEDLTPDVFRVHLTGRREAIAHAPGQYLELKLDEQTWVPFSIANAHADDGVIELHVQHWPERSNSARL
RELLVHAARLTVRLPNGGCVLDTRSRRPLTLIAAGTGFAQMKAIVEAALANDHPGPIRLWWAVKSPRDLYLESLALEWAA
THPHLDVHTVVEDSEPALSAPAGVTRHIGRIDEVLAECHGDISGDDVYLSGSPGMVYACIDTLAPLGLDPERVFSDVFAY
APRVPLIPLPEEVSEKAPLSPGERRAEDDA
>Mature_269_residues
TSKTLTCQVTSVEDLTPDVFRVHLTGRREAIAHAPGQYLELKLDEQTWVPFSIANAHADDGVIELHVQHWPERSNSARLR
ELLVHAARLTVRLPNGGCVLDTRSRRPLTLIAAGTGFAQMKAIVEAALANDHPGPIRLWWAVKSPRDLYLESLALEWAAT
HPHLDVHTVVEDSEPALSAPAGVTRHIGRIDEVLAECHGDISGDDVYLSGSPGMVYACIDTLAPLGLDPERVFSDVFAYA
PRVPLIPLPEEVSEKAPLSPGERRAEDDA

Specific function: Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduces the complexed Fe(3+) iron of siderophores to Fe(2+), thus releasing it from the chelator [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=241, Percent_Identity=31.5352697095436, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: =1.16.1.3; =1.5.1.29 [H]

Molecular weight: Translated: 29526; Mature: 29395

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKTLTCQVTSVEDLTPDVFRVHLTGRREAIAHAPGQYLELKLDEQTWVPFSIANAHAD
CCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHCCCCCEEEEEECCCCCCCEEECCCCCC
DGVIELHVQHWPERSNSARLRELLVHAARLTVRLPNGGCVLDTRSRRPLTLIAAGTGFAQ
CCEEEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCEEEEEECCCHHH
MKAIVEAALANDHPGPIRLWWAVKSPRDLYLESLALEWAATHPHLDVHTVVEDSEPALSA
HHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
PAGVTRHIGRIDEVLAECHGDISGDDVYLSGSPGMVYACIDTLAPLGLDPERVFSDVFAY
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
APRVPLIPLPEEVSEKAPLSPGERRAEDDA
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSKTLTCQVTSVEDLTPDVFRVHLTGRREAIAHAPGQYLELKLDEQTWVPFSIANAHAD
CCCEEEEEEECCCCCCCCEEEEEECCCHHHHHCCCCCEEEEEECCCCCCCEEECCCCCC
DGVIELHVQHWPERSNSARLRELLVHAARLTVRLPNGGCVLDTRSRRPLTLIAAGTGFAQ
CCEEEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCEEEEEECCCHHH
MKAIVEAALANDHPGPIRLWWAVKSPRDLYLESLALEWAATHPHLDVHTVVEDSEPALSA
HHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
PAGVTRHIGRIDEVLAECHGDISGDDVYLSGSPGMVYACIDTLAPLGLDPERVFSDVFAY
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
APRVPLIPLPEEVSEKAPLSPGERRAEDDA
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]