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The map label for this gene is emrB [H]

Identifier: 92112686

GI number: 92112686

Start: 614684

End: 616234

Strand: Direct

Name: emrB [H]

Synonym: Csal_0553

Alternate gene names: 92112686

Gene position: 614684-616234 (Clockwise)

Preceding gene: 92112685

Following gene: 92112688

Centisome position: 16.63

GC content: 65.51

Gene sequence:

>1551_bases
ATGGCGGAGGCGGTGACCGATACGGCGTCAGGGCCCCGGGAGGCTCCTTCGCGGCGCACGCAACTGGGCTTCGTGGCCGC
CGTGTTCGGCATGTTCATGGCGATTCTGGACATCCAGATCGTCGCCAGTTCCCTCAACGAGATCCAGGCCGGGGTCTCGG
CCAGCGCCGACGAGATCTCCTGGGTCCAGACATCGTATCTGATCGCCGAGATCATCATGATCCCGCTGTCGGGGATGCTG
ACGCGGATCCTTTCCACCCGGGTGGCGTTCGTGGTCTCGTGCCTGGGCTTCACCCTGGCAAGCTTGGGCTGTGCCATGGC
CAACTCCATCGAGTCGCTGATCGTGCTGCGCGCGATTCAGGGCTTCATGGGCGGGGCGATGATCCCCATTGCCTATGCCA
TCAGCTTCAGTGTCTTCCCGCGGCGCATGATGGGCTCGGTGCAGGCCGTGATGGGGCTGGTGGCGACCCTGGCACCGTCG
GTGGGCCCGACCGTGGGCGGTTACATTACCGAGTTCGCGAGCTGGCACTGGCTGTTCCTGGCCAACGCGGTGCCCGGCGT
TCTGGTGTCGCTGGCGGTATGGCGGTTGCTGGACATCGATCGTCCCGATCGGCGTGCCCTGCGGCATCTGGACCTCATCG
GGCTGGCCCTGCTGGCGCTCTTCCTGGGCACGCTTGAGTTTTCGCTCGAAGAAGGCCCCGGTGACGACTGGTTCGCCAGC
CGCACGATCCTCATCGCTGCCTTCGTCTGCGCGCTCGCGTCGCTGGGATTCTTCTGGCGGACCCTGCGCCACCGCCACCC
GATCGTCGATTTGCGCGCCTTCGCCAACCTCAACTTCGCCGTGGGGGCGTGGCTGGGGTTCGTGGTAGGCATCGGGCTCT
ATGGCCTGGTCTATCTGCTGCCGTTGTTCCTGGGCAATGTGCGAGGCTATTCGGCGCTGCAGATCGGTGAGGTCATGATC
GTGACCGGGGTGGCGATGTTCCTGACCGCTCCCCTGGTAGGGCGCGCTTCCGACAAGGTCGACCTGCGGGTGCTGCTGTT
GCTGGGCATGACGCTGACCGGCATCGGCACCATCATGAATGCCAACCTGACCGCCGAGGCCGGGTTCGACGCGTTCTTCT
GGCCGCAGGTGGTGCGCGGCGTGGGCCTGGTGATGTGCCTGATTCCCACCTCGCGTATCGCCTTCGGCACCTTGCCGCCG
AGCGAGGTCGGCAACGCCAGCGGCCTGTTCAACGTGATGCGCAACCTGGGCGGCGCGGTGGGGCTGGCGCTGCTGGACAC
GATCCGCGACTGGCGCGAGGACTACCACTGGAACCAGTTGATTCCCGCCATCGACAGCGGTCGCGAGGTGGTCAATGCCG
CCCAGGCGCAATATCAGCAGCTGCTGGCCGCCACCGGCGACCCGCAGACCGCCTCTCTCGGCATGCTGTCGCGGACCCTG
CTCGAGCAGGCCATGGTCATGGCCTACAACGATATTTTCCTGTGGCTGGGCATGCTGTATCTACTGGTGGCGCCGTTGAT
CCTGCTGTTGAAACGTCCCGCTCACGCCTGA

Upstream 100 bases:

>100_bases
GTGACGGGACCGGAAGAAGCCCTGGGACGCCTGCGCGACGGCCTCTCGGTGGTGCCAACGGTGGATACGCGCGATCTCGA
CCCCGAGGACGACGCCTGAC

Downstream 100 bases:

>100_bases
CGGCGCGGCATGCCGCGGTGACCGGCGGCTAGTAATGGGGCGGGATCTCGTCGTGTGGGGCCGGTGCGCTGTCGTCGCCC
AGCGTCTCGACGTTCTGGCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MAEAVTDTASGPREAPSRRTQLGFVAAVFGMFMAILDIQIVASSLNEIQAGVSASADEISWVQTSYLIAEIIMIPLSGML
TRILSTRVAFVVSCLGFTLASLGCAMANSIESLIVLRAIQGFMGGAMIPIAYAISFSVFPRRMMGSVQAVMGLVATLAPS
VGPTVGGYITEFASWHWLFLANAVPGVLVSLAVWRLLDIDRPDRRALRHLDLIGLALLALFLGTLEFSLEEGPGDDWFAS
RTILIAAFVCALASLGFFWRTLRHRHPIVDLRAFANLNFAVGAWLGFVVGIGLYGLVYLLPLFLGNVRGYSALQIGEVMI
VTGVAMFLTAPLVGRASDKVDLRVLLLLGMTLTGIGTIMNANLTAEAGFDAFFWPQVVRGVGLVMCLIPTSRIAFGTLPP
SEVGNASGLFNVMRNLGGAVGLALLDTIRDWREDYHWNQLIPAIDSGREVVNAAQAQYQQLLAATGDPQTASLGMLSRTL
LEQAMVMAYNDIFLWLGMLYLLVAPLILLLKRPAHA

Sequences:

>Translated_516_residues
MAEAVTDTASGPREAPSRRTQLGFVAAVFGMFMAILDIQIVASSLNEIQAGVSASADEISWVQTSYLIAEIIMIPLSGML
TRILSTRVAFVVSCLGFTLASLGCAMANSIESLIVLRAIQGFMGGAMIPIAYAISFSVFPRRMMGSVQAVMGLVATLAPS
VGPTVGGYITEFASWHWLFLANAVPGVLVSLAVWRLLDIDRPDRRALRHLDLIGLALLALFLGTLEFSLEEGPGDDWFAS
RTILIAAFVCALASLGFFWRTLRHRHPIVDLRAFANLNFAVGAWLGFVVGIGLYGLVYLLPLFLGNVRGYSALQIGEVMI
VTGVAMFLTAPLVGRASDKVDLRVLLLLGMTLTGIGTIMNANLTAEAGFDAFFWPQVVRGVGLVMCLIPTSRIAFGTLPP
SEVGNASGLFNVMRNLGGAVGLALLDTIRDWREDYHWNQLIPAIDSGREVVNAAQAQYQQLLAATGDPQTASLGMLSRTL
LEQAMVMAYNDIFLWLGMLYLLVAPLILLLKRPAHA
>Mature_515_residues
AEAVTDTASGPREAPSRRTQLGFVAAVFGMFMAILDIQIVASSLNEIQAGVSASADEISWVQTSYLIAEIIMIPLSGMLT
RILSTRVAFVVSCLGFTLASLGCAMANSIESLIVLRAIQGFMGGAMIPIAYAISFSVFPRRMMGSVQAVMGLVATLAPSV
GPTVGGYITEFASWHWLFLANAVPGVLVSLAVWRLLDIDRPDRRALRHLDLIGLALLALFLGTLEFSLEEGPGDDWFASR
TILIAAFVCALASLGFFWRTLRHRHPIVDLRAFANLNFAVGAWLGFVVGIGLYGLVYLLPLFLGNVRGYSALQIGEVMIV
TGVAMFLTAPLVGRASDKVDLRVLLLLGMTLTGIGTIMNANLTAEAGFDAFFWPQVVRGVGLVMCLIPTSRIAFGTLPPS
EVGNASGLFNVMRNLGGAVGLALLDTIRDWREDYHWNQLIPAIDSGREVVNAAQAQYQQLLAATGDPQTASLGMLSRTLL
EQAMVMAYNDIFLWLGMLYLLVAPLILLLKRPAHA

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=29.9009900990099, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788710, Length=493, Percent_Identity=28.8032454361055, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081983, Length=409, Percent_Identity=26.8948655256724, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1790195, Length=337, Percent_Identity=27.5964391691395, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788392, Length=305, Percent_Identity=30.1639344262295, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6325455, Length=125, Percent_Identity=30.4, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55569; Mature: 55438

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAVTDTASGPREAPSRRTQLGFVAAVFGMFMAILDIQIVASSLNEIQAGVSASADEIS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
WVQTSYLIAEIIMIPLSGMLTRILSTRVAFVVSCLGFTLASLGCAMANSIESLIVLRAIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFMGGAMIPIAYAISFSVFPRRMMGSVQAVMGLVATLAPSVGPTVGGYITEFASWHWLFL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ANAVPGVLVSLAVWRLLDIDRPDRRALRHLDLIGLALLALFLGTLEFSLEEGPGDDWFAS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RTILIAAFVCALASLGFFWRTLRHRHPIVDLRAFANLNFAVGAWLGFVVGIGLYGLVYLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
PLFLGNVRGYSALQIGEVMIVTGVAMFLTAPLVGRASDKVDLRVLLLLGMTLTGIGTIMN
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
ANLTAEAGFDAFFWPQVVRGVGLVMCLIPTSRIAFGTLPPSEVGNASGLFNVMRNLGGAV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCCHHCCCHHHHHHHHHHHHHHH
GLALLDTIRDWREDYHWNQLIPAIDSGREVVNAAQAQYQQLLAATGDPQTASLGMLSRTL
HHHHHHHHHHHHHCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEQAMVMAYNDIFLWLGMLYLLVAPLILLLKRPAHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEAVTDTASGPREAPSRRTQLGFVAAVFGMFMAILDIQIVASSLNEIQAGVSASADEIS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]