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The map label for this gene is ispH

Identifier: 92112617

GI number: 92112617

Start: 533843

End: 534796

Strand: Direct

Name: ispH

Synonym: Csal_0484

Alternate gene names: 92112617

Gene position: 533843-534796 (Clockwise)

Preceding gene: 92112616

Following gene: 92112618

Centisome position: 14.44

GC content: 65.2

Gene sequence:

>954_bases
ATGCAAATCAAGCTGGCCAATCCACGCGGTTTCTGCGCTGGCGTCGACCGCGCCATCGAGATCGTCAATCGTGCGCTCGA
CGTCTTCGGGCCGCCCATCTACGTACGTCATGAAGTTGTGCACAACCGCTTCGTGGTGGACAGCCTGCGCGAGCGCGGGG
CGGTGTTCGTCGAGGAGCTGCATGAAGTGCCCGACGACGTGATCGTCATCTTCTCCGCCCACGGGGTCTCGCGCGCGGTG
CAGCAGGAAGCCGAGCGGCGTGGCCTGCGCATCTTCGACGCCACCTGTCCGCTGGTCACCAAGGTGCACATGGAAGTCCT
GCGCTATGCCAAGCGGGGCCAGGAATGCGTGCTCATCGGGCATGCCGGCCATCCCGAGGTCGAAGGCACCATGGGCCGCT
ACGATACCTCGTTCGGCGGGCAGATCTATCTGGTCGAGGACGAGGCGGACGTGGAGAACCTCGAGGTCAACGGTCCCGAG
CGGTTGGCGTTCGTCACCCAGACCACGCTCTCCATGGATGACACCTCGCGGGTCATCGATGCCCTGCGCGCCAAGTTTCC
GCAGATCGACGGGCCGCGCAAGGATGACATCTGCTATGCCACCCAGAATCGTCAGGACGCGGTGCGCGGGCTGGCCAGCG
AGAGCGACCTGGTGCTGGTGGTGGGCAGCCCCAACAGTTCCAACTCCAATCGCTTGCGTGAATTGGCCGAGCGCACCGGC
ACACCGGCATACCTGATCGATACCGCCGAGCAGATCCGTCCCGAATGGCTGACCGACGTGAGCGCCATCGGCATCACGGC
CGGCGCCAGCGCGCCGGAGGTACTCGTGCAGGCGGTCATCGAGCGACTCCAGTCACTGGGCGCCGAGGCACCCGACGAGC
TTGCCGGACAAGCGGAAAACATCACCTTCTCCATGCCCCAGGAGCTGCGCGAGCGCGTCATCGCCAGCGAGTGA

Upstream 100 bases:

>100_bases
GTGGAAGTGATCGATGTCCGCCCGGCCACGACCCATTAAGCTTTGACGGGCGTCCGTCGCCCATCGACACGATGCCGCCG
TGCATCGAGGCAAGGTAAGC

Downstream 100 bases:

>100_bases
TCCCGCGCGACACGCCCGATGCGAGCTCGGGCGTGTCGCGGCGTGCGTTACAATTGGTTGCAAAATACGCTACATTGGAG
GGGCGCTTGGGGCCTCTCTC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MQIKLANPRGFCAGVDRAIEIVNRALDVFGPPIYVRHEVVHNRFVVDSLRERGAVFVEELHEVPDDVIVIFSAHGVSRAV
QQEAERRGLRIFDATCPLVTKVHMEVLRYAKRGQECVLIGHAGHPEVEGTMGRYDTSFGGQIYLVEDEADVENLEVNGPE
RLAFVTQTTLSMDDTSRVIDALRAKFPQIDGPRKDDICYATQNRQDAVRGLASESDLVLVVGSPNSSNSNRLRELAERTG
TPAYLIDTAEQIRPEWLTDVSAIGITAGASAPEVLVQAVIERLQSLGAEAPDELAGQAENITFSMPQELRERVIASE

Sequences:

>Translated_317_residues
MQIKLANPRGFCAGVDRAIEIVNRALDVFGPPIYVRHEVVHNRFVVDSLRERGAVFVEELHEVPDDVIVIFSAHGVSRAV
QQEAERRGLRIFDATCPLVTKVHMEVLRYAKRGQECVLIGHAGHPEVEGTMGRYDTSFGGQIYLVEDEADVENLEVNGPE
RLAFVTQTTLSMDDTSRVIDALRAKFPQIDGPRKDDICYATQNRQDAVRGLASESDLVLVVGSPNSSNSNRLRELAERTG
TPAYLIDTAEQIRPEWLTDVSAIGITAGASAPEVLVQAVIERLQSLGAEAPDELAGQAENITFSMPQELRERVIASE
>Mature_317_residues
MQIKLANPRGFCAGVDRAIEIVNRALDVFGPPIYVRHEVVHNRFVVDSLRERGAVFVEELHEVPDDVIVIFSAHGVSRAV
QQEAERRGLRIFDATCPLVTKVHMEVLRYAKRGQECVLIGHAGHPEVEGTMGRYDTSFGGQIYLVEDEADVENLEVNGPE
RLAFVTQTTLSMDDTSRVIDALRAKFPQIDGPRKDDICYATQNRQDAVRGLASESDLVLVVGSPNSSNSNRLRELAERTG
TPAYLIDTAEQIRPEWLTDVSAIGITAGASAPEVLVQAVIERLQSLGAEAPDELAGQAENITFSMPQELRERVIASE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=66.7741935483871, Blast_Score=426, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CHRSD (Q1R0B2)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_572545.1
- STRING:   Q1R0B2
- GeneID:   4026508
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_0484
- NMPDR:   fig|290398.4.peg.43
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   MDVPGFY
- BioCyc:   CSAL290398:CSAL_0484-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34914; Mature: 34914

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKLANPRGFCAGVDRAIEIVNRALDVFGPPIYVRHEVVHNRFVVDSLRERGAVFVEEL
CEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
HEVPDDVIVIFSAHGVSRAVQQEAERRGLRIFDATCPLVTKVHMEVLRYAKRGQECVLIG
HHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEE
HAGHPEVEGTMGRYDTSFGGQIYLVEDEADVENLEVNGPERLAFVTQTTLSMDDTSRVID
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHEEEEEEHHCCCCHHHHHHH
ALRAKFPQIDGPRKDDICYATQNRQDAVRGLASESDLVLVVGSPNSSNSNRLRELAERTG
HHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCC
TPAYLIDTAEQIRPEWLTDVSAIGITAGASAPEVLVQAVIERLQSLGAEAPDELAGQAEN
CCEEEECCHHHHCHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
ITFSMPQELRERVIASE
EEEECCHHHHHHHCCCC
>Mature Secondary Structure
MQIKLANPRGFCAGVDRAIEIVNRALDVFGPPIYVRHEVVHNRFVVDSLRERGAVFVEEL
CEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
HEVPDDVIVIFSAHGVSRAVQQEAERRGLRIFDATCPLVTKVHMEVLRYAKRGQECVLIG
HHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEE
HAGHPEVEGTMGRYDTSFGGQIYLVEDEADVENLEVNGPERLAFVTQTTLSMDDTSRVID
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHEEEEEEHHCCCCHHHHHHH
ALRAKFPQIDGPRKDDICYATQNRQDAVRGLASESDLVLVVGSPNSSNSNRLRELAERTG
HHHHHCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCC
TPAYLIDTAEQIRPEWLTDVSAIGITAGASAPEVLVQAVIERLQSLGAEAPDELAGQAEN
CCEEEECCHHHHCHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
ITFSMPQELRERVIASE
EEEECCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA