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The map label for this gene is nrdF [H]

Identifier: 92112513

GI number: 92112513

Start: 424550

End: 425530

Strand: Direct

Name: nrdF [H]

Synonym: Csal_0380

Alternate gene names: 92112513

Gene position: 424550-425530 (Clockwise)

Preceding gene: 92112512

Following gene: 92112515

Centisome position: 11.48

GC content: 62.08

Gene sequence:

>981_bases
ATGACCGCTACCGCGACACCGCGTCACCTGACGCGCGTCGATGCCATCAACTGGAACCGTCTGCAGGACGAGAAGGACCT
CGAGGTCTGGAACCGTCTCACCGCCAATTTCTGGCTGCCCGAGAAGGTGCCGCTGTCCAACGACGTGAGCGCGTGGAACA
CGTTGACCGAGCAGGAGCAGACGTTGACCATCCGTGTCTTCACGGGGCTCACGCTGCTCGACACCATCCAGGGAACCATC
GGCGCACCGACGCTGATCGAGGACGCCGTGACGCCCCACGAGGAGGCGGTGTTCACCAACATCAGCTTCATGGAGTCGGT
GCACGCGCGCTCCTACAGCTCGATCTTCTCTACGCTGTGCGCCACCCGCGACGTCGACGACGCCTACCGGTGGAGCGAGG
AGAATCCGCACCTGCAGAACAAGGCCGAGCTGATTCTCAACCGCTACCGCATGGACGATCCGCTGATGCGCAAGGTCGCC
AGCGTCTTCCTCGAGTCGTTCTTGTTCTACTCCGGCTTCTACCTGCCGATGTACTGGGCCAGTCGCGGCAAGCTGACCAA
TACCGCCGACCTGATTCGCCTGATCATTCGCGACGAGGCGGTCCACGGCTACTATATCGGCTACAAGTTCCAGCAGGCGC
TGGCCAGGGAAAGTGCCGAGCGCCAGCAGGAGATCAAGGATGCCACCTTCGACCTGCTGCTCGAGCTCTACGACAACGAG
GTGCACTACACCGAATCGCTCTACGACGACGTGGGGCTGACCGAAGAGGTCAAGGCGTTCTTGCATTACAACGCCAACAA
GGCGTTGATGAACCTGGGCTACGAGCCGCTGTTTCCCGGCGCCACCGAAGCGGTCAATCCCGCGATCCTGGCGGCGCTCT
CGCCGGGAGCCGAGGAAAACCACGACTTCTTCTCCGGCTCCGGTTCTTCCTACGTCATCGGGCGTTCGGAGCGCACCGAG
GACGAAGACTGGGCGTTCTGA

Upstream 100 bases:

>100_bases
TGACGGCAGGCGACGTGCCCGAGCCATAGACACTTCCATCACGATCATCGGCGAGGCGTGGCGACCGGCCAGCCTCGCCG
TCGGCAATGAGAGAACCTCC

Downstream 100 bases:

>100_bases
GCCGCGCCATCATCGGCGGTGATCGATAGACGCCACGTGCTGACCGCCTCGTCCAGCCATGCCTGGAAGGCGCGCACCTT
GGGCAGCCCGGCATTGCCGG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTATATPRHLTRVDAINWNRLQDEKDLEVWNRLTANFWLPEKVPLSNDVSAWNTLTEQEQTLTIRVFTGLTLLDTIQGTI
GAPTLIEDAVTPHEEAVFTNISFMESVHARSYSSIFSTLCATRDVDDAYRWSEENPHLQNKAELILNRYRMDDPLMRKVA
SVFLESFLFYSGFYLPMYWASRGKLTNTADLIRLIIRDEAVHGYYIGYKFQQALARESAERQQEIKDATFDLLLELYDNE
VHYTESLYDDVGLTEEVKAFLHYNANKALMNLGYEPLFPGATEAVNPAILAALSPGAEENHDFFSGSGSSYVIGRSERTE
DEDWAF

Sequences:

>Translated_326_residues
MTATATPRHLTRVDAINWNRLQDEKDLEVWNRLTANFWLPEKVPLSNDVSAWNTLTEQEQTLTIRVFTGLTLLDTIQGTI
GAPTLIEDAVTPHEEAVFTNISFMESVHARSYSSIFSTLCATRDVDDAYRWSEENPHLQNKAELILNRYRMDDPLMRKVA
SVFLESFLFYSGFYLPMYWASRGKLTNTADLIRLIIRDEAVHGYYIGYKFQQALARESAERQQEIKDATFDLLLELYDNE
VHYTESLYDDVGLTEEVKAFLHYNANKALMNLGYEPLFPGATEAVNPAILAALSPGAEENHDFFSGSGSSYVIGRSERTE
DEDWAF
>Mature_325_residues
TATATPRHLTRVDAINWNRLQDEKDLEVWNRLTANFWLPEKVPLSNDVSAWNTLTEQEQTLTIRVFTGLTLLDTIQGTIG
APTLIEDAVTPHEEAVFTNISFMESVHARSYSSIFSTLCATRDVDDAYRWSEENPHLQNKAELILNRYRMDDPLMRKVAS
VFLESFLFYSGFYLPMYWASRGKLTNTADLIRLIIRDEAVHGYYIGYKFQQALARESAERQQEIKDATFDLLLELYDNEV
HYTESLYDDVGLTEEVKAFLHYNANKALMNLGYEPLFPGATEAVNPAILAALSPGAEENHDFFSGSGSSYVIGRSERTED
EDWAF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=317, Percent_Identity=72.8706624605678, Blast_Score=492, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 37240; Mature: 37109

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATATPRHLTRVDAINWNRLQDEKDLEVWNRLTANFWLPEKVPLSNDVSAWNTLTEQEQ
CCCCCCCHHHHHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCC
TLTIRVFTGLTLLDTIQGTIGAPTLIEDAVTPHEEAVFTNISFMESVHARSYSSIFSTLC
EEEEEEECCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATRDVDDAYRWSEENPHLQNKAELILNRYRMDDPLMRKVASVFLESFLFYSGFYLPMYWA
HCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEC
SRGKLTNTADLIRLIIRDEAVHGYYIGYKFQQALARESAERQQEIKDATFDLLLELYDNE
CCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VHYTESLYDDVGLTEEVKAFLHYNANKALMNLGYEPLFPGATEAVNPAILAALSPGAEEN
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHCCHHHEEEECCCCCCC
HDFFSGSGSSYVIGRSERTEDEDWAF
CCCCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TATATPRHLTRVDAINWNRLQDEKDLEVWNRLTANFWLPEKVPLSNDVSAWNTLTEQEQ
CCCCCCHHHHHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCC
TLTIRVFTGLTLLDTIQGTIGAPTLIEDAVTPHEEAVFTNISFMESVHARSYSSIFSTLC
EEEEEEECCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATRDVDDAYRWSEENPHLQNKAELILNRYRMDDPLMRKVASVFLESFLFYSGFYLPMYWA
HCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEC
SRGKLTNTADLIRLIIRDEAVHGYYIGYKFQQALARESAERQQEIKDATFDLLLELYDNE
CCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VHYTESLYDDVGLTEEVKAFLHYNANKALMNLGYEPLFPGATEAVNPAILAALSPGAEEN
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHCCHHHEEEECCCCCCC
HDFFSGSGSSYVIGRSERTEDEDWAF
CCCCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8195103; 11677609; 2691838; 2548994; 9748343 [H]