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The map label for this gene is rhmD [H]

Identifier: 92112499

GI number: 92112499

Start: 410461

End: 411783

Strand: Direct

Name: rhmD [H]

Synonym: Csal_0366

Alternate gene names: 92112499

Gene position: 410461-411783 (Clockwise)

Preceding gene: 92112497

Following gene: 92112500

Centisome position: 11.1

GC content: 64.17

Gene sequence:

>1323_bases
GTGCCTGTCATCACCACCATCGAACTGGTAGATCTACGTTTCCCAACCTCCGAGAGCAACGCCGGCTCCGACGCGGTGAA
CAAGGACGCCGACTATTCCGCGGCCTATCTCAGGCTGGCAACCGATGACAGCACACTTTATGGCTGCGGGTTTACCTTCA
CCATCGGGCGCGGCAACGAGATCTGCCTTCAGGCCATGGAGGTCCTGGCCGCTAAGCTCGTCGGTCGGGACGTGGCCCAG
GTGTGCCGGGCGCCGGGAACAGTCTACCGGGAGCTCGCCGAGGATTCGCAGTTGCGCTGGCTGGGCCCCGACAAGGGGGT
CGTGCATCTGGCCCGGGCGGCGGTGATGAACGCCGTTTGGGACCTGGCTGCCCGCCATGCGGAGGTGCCGCTGTGGCGGC
TGATCTGTGAGATGGACCCGGAGGTGCTCGTCGAGCAGTTGGATCTAAGCTATCTCGGCGATGTGCTGAGCGCCGGGGAG
GCGGTGGCGATGCTCGAGGAGCAGCGGCCCTTCCACCAGGCGCGAATCGCCGAACTGAGCGAACAAGGGTTTCCCTGCTA
TACCACATCGCCTGGGTGGCTGGGCTACTCGGACGACAAGCTTGCCCGGCTCTGCCGAGAGGCCATGGACGAGGGCTACC
AGCACATCAAGCTTAAGGTCGGTGGCGATCCCGCTGATGACGAGCGCCGGTTGGGTATCGCGCGCTCGGTGATCGGCCCC
GACAAGATGTTGATGATCGACGCCAATCAGATCTGGGAAGTCCCCGAGGCGATCGAGCGCGTCGAGGCCCTGGCGCACCA
TGGCCTGCTGTGGATCGAGGAGCCGACCAGCCCGGACGATGTGCTGGGGCATCGACGTATCGCCGACAGTGTTCGGCAGC
GGGGGATCGGGGTTGCCACCGGCGAGCACGGTATGAATCGGGTGCTGTTCAAGCAGTTGCTGCAGGCGGACGCGGTGGAC
TTCGTACAGATCGACTCGTGCCGGCTCGCCAGTATCAACGAGATCATCCCGGTGCTGCTGATGGCGAAGAAGTCTGGCAA
GCCCGTCTGCCCGCATGCTGGCGGGGTCGGGCTATGCGAGCTGGTCAGTCATCTGGTGATGATCGATCATATCGCGGTAT
CGGGCCCTGTGGCGGGGCGCGTGGCCGAATTCGTCGATCATCTCCACGAGCACTTCGTGGACCCCTGCCACGTCAGTCAG
GGCGCTTATGTCTTGCCGACTCGCCCAGGATATGCGGCAGAGATCAAGGCCGAGACGCTGGCCGCCTGGGCATGGCCGGA
CGGTACTGAGTGGGTCAAGCGTCGACAGGTGATGACGGCATGA

Upstream 100 bases:

>100_bases
GGCGGCATGCAATGGGTCTCCGGCTCGGGGTCGCGGGTAACCATTCTATCGGAATTGTCGCTGGCACCTTAATACATCTG
ATTAATCAGGAGGGCATGAC

Downstream 100 bases:

>100_bases
TCAACGGACCGCTGCTGCATCCGCCTCTTTTGCATGCTCTCGCCGCCGCGGGGCACGGCGCCAAGATACTCGTCGCCGAT
GCGCTCTACCCGCACTCCAC

Product: mandelate racemase/muconate lactonizing enzyme

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MPVITTIELVDLRFPTSESNAGSDAVNKDADYSAAYLRLATDDSTLYGCGFTFTIGRGNEICLQAMEVLAAKLVGRDVAQ
VCRAPGTVYRELAEDSQLRWLGPDKGVVHLARAAVMNAVWDLAARHAEVPLWRLICEMDPEVLVEQLDLSYLGDVLSAGE
AVAMLEEQRPFHQARIAELSEQGFPCYTTSPGWLGYSDDKLARLCREAMDEGYQHIKLKVGGDPADDERRLGIARSVIGP
DKMLMIDANQIWEVPEAIERVEALAHHGLLWIEEPTSPDDVLGHRRIADSVRQRGIGVATGEHGMNRVLFKQLLQADAVD
FVQIDSCRLASINEIIPVLLMAKKSGKPVCPHAGGVGLCELVSHLVMIDHIAVSGPVAGRVAEFVDHLHEHFVDPCHVSQ
GAYVLPTRPGYAAEIKAETLAAWAWPDGTEWVKRRQVMTA

Sequences:

>Translated_440_residues
MPVITTIELVDLRFPTSESNAGSDAVNKDADYSAAYLRLATDDSTLYGCGFTFTIGRGNEICLQAMEVLAAKLVGRDVAQ
VCRAPGTVYRELAEDSQLRWLGPDKGVVHLARAAVMNAVWDLAARHAEVPLWRLICEMDPEVLVEQLDLSYLGDVLSAGE
AVAMLEEQRPFHQARIAELSEQGFPCYTTSPGWLGYSDDKLARLCREAMDEGYQHIKLKVGGDPADDERRLGIARSVIGP
DKMLMIDANQIWEVPEAIERVEALAHHGLLWIEEPTSPDDVLGHRRIADSVRQRGIGVATGEHGMNRVLFKQLLQADAVD
FVQIDSCRLASINEIIPVLLMAKKSGKPVCPHAGGVGLCELVSHLVMIDHIAVSGPVAGRVAEFVDHLHEHFVDPCHVSQ
GAYVLPTRPGYAAEIKAETLAAWAWPDGTEWVKRRQVMTA
>Mature_439_residues
PVITTIELVDLRFPTSESNAGSDAVNKDADYSAAYLRLATDDSTLYGCGFTFTIGRGNEICLQAMEVLAAKLVGRDVAQV
CRAPGTVYRELAEDSQLRWLGPDKGVVHLARAAVMNAVWDLAARHAEVPLWRLICEMDPEVLVEQLDLSYLGDVLSAGEA
VAMLEEQRPFHQARIAELSEQGFPCYTTSPGWLGYSDDKLARLCREAMDEGYQHIKLKVGGDPADDERRLGIARSVIGPD
KMLMIDANQIWEVPEAIERVEALAHHGLLWIEEPTSPDDVLGHRRIADSVRQRGIGVATGEHGMNRVLFKQLLQADAVDF
VQIDSCRLASINEIIPVLLMAKKSGKPVCPHAGGVGLCELVSHLVMIDHIAVSGPVAGRVAEFVDHLHEHFVDPCHVSQG
AYVLPTRPGYAAEIKAETLAAWAWPDGTEWVKRRQVMTA

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=431, Percent_Identity=51.0440835266821, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI42544117, Length=419, Percent_Identity=49.1646778042959, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI186972148, Length=339, Percent_Identity=53.3923303834808, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 48296; Mature: 48165

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITTIELVDLRFPTSESNAGSDAVNKDADYSAAYLRLATDDSTLYGCGFTFTIGRGNE
CCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCEEEEECCCCH
ICLQAMEVLAAKLVGRDVAQVCRAPGTVYRELAEDSQLRWLGPDKGVVHLARAAVMNAVW
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
DLAARHAEVPLWRLICEMDPEVLVEQLDLSYLGDVLSAGEAVAMLEEQRPFHQARIAELS
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
EQGFPCYTTSPGWLGYSDDKLARLCREAMDEGYQHIKLKVGGDPADDERRLGIARSVIGP
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCC
DKMLMIDANQIWEVPEAIERVEALAHHGLLWIEEPTSPDDVLGHRRIADSVRQRGIGVAT
CCEEEEECHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEC
GEHGMNRVLFKQLLQADAVDFVQIDSCRLASINEIIPVLLMAKKSGKPVCPHAGGVGLCE
CCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
LVSHLVMIDHIAVSGPVAGRVAEFVDHLHEHFVDPCHVSQGAYVLPTRPGYAAEIKAETL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEECHHHE
AAWAWPDGTEWVKRRQVMTA
EEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PVITTIELVDLRFPTSESNAGSDAVNKDADYSAAYLRLATDDSTLYGCGFTFTIGRGNE
CCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCEEEEECCCCH
ICLQAMEVLAAKLVGRDVAQVCRAPGTVYRELAEDSQLRWLGPDKGVVHLARAAVMNAVW
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
DLAARHAEVPLWRLICEMDPEVLVEQLDLSYLGDVLSAGEAVAMLEEQRPFHQARIAELS
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
EQGFPCYTTSPGWLGYSDDKLARLCREAMDEGYQHIKLKVGGDPADDERRLGIARSVIGP
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCC
DKMLMIDANQIWEVPEAIERVEALAHHGLLWIEEPTSPDDVLGHRRIADSVRQRGIGVAT
CCEEEEECHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEC
GEHGMNRVLFKQLLQADAVDFVQIDSCRLASINEIIPVLLMAKKSGKPVCPHAGGVGLCE
CCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
LVSHLVMIDHIAVSGPVAGRVAEFVDHLHEHFVDPCHVSQGAYVLPTRPGYAAEIKAETL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEECHHHE
AAWAWPDGTEWVKRRQVMTA
EEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA