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The map label for this gene is pcaH [H]

Identifier: 92112445

GI number: 92112445

Start: 351206

End: 351946

Strand: Direct

Name: pcaH [H]

Synonym: Csal_0311

Alternate gene names: 92112445

Gene position: 351206-351946 (Clockwise)

Preceding gene: 92112444

Following gene: 92112446

Centisome position: 9.5

GC content: 65.05

Gene sequence:

>741_bases
ATGCCTCTCAACGATAACAAGCGCTTCGTCGAGCGCGACCGTAACTGGCACCCCCCGGCCTATGCGCCGGGCTACAAGAC
GACGGTGGCGCGCTCCCCCAGCCAGGCCTACGTCAGTCTGCAGACGCCGAGTGTCTCGGAGCTCGCCGGCCCGGATTTCA
CGCGCCTGCGCATGGGCCCGCACGACAACGACCTGCTGCTCAACTTCCGTGCCGACGACGAGGCACGGGCGGGGCTGCCC
ATCGGCGAACGCATCCTGCTGTTCGGACGCGTCGTCGATCAGTTCGGCAAGCCGGTGCCCAATACCCTGGTGGAAATGTG
GCAGGCCAACGCCGGCGGCCGTTACCGTCACAAGAAGGATGGCTACCTGGCACCGCTCGATCCCAATTTCGGCGGCGTGG
GGCGCTGCCTGAGCGACGACGAGGGCTGGTACCGTTTCCGCACCATCAAGCCCGGCCCGTACCCCTGGCCCAACGACATC
AACAGCTGGCGGCCGGCGCACATACACGTCTCGGTGATGGGGCCGTCGATCTCCACGCGCCTGATTACCCAGATGTACTT
CGAGGGCGACCCGCTGATTCCGCTGTGCCCCATCGTGCAGACCCTGCGCGATCCCGAAGCCGTCGAGACAATGGTCGGTC
GTCTCGACATGGCGCGCAGCCGCTCGATGGATTGCCTGGCCTACCGTTTCGACCTGGTCGTTCGCGGTGAACTGCAAACC
TATTTCGAGAATCAGGGGTAA

Upstream 100 bases:

>100_bases
GGGCAGCCATTTCAATTGGCAGCTGGGGCCCAGCGCGGACAATGAGGCATGTCATCGCGGCGTTCGCTGGCGCCGCGGTC
CTTTCGATCAGGAGACTCGC

Downstream 100 bases:

>100_bases
ACCATGCAACAGCCGAATTCCCAGCCCAGCGCCGCGCTCATGCTGCGCGAAACCGCGTCGCAGACGGCCGGTCCCTACGT
GCACATCGGCCTGGCCCTGG

Product: protocatechuate 3,4-dioxygenase subunit beta

Products: NA

Alternate protein names: 3,4-PCD [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MPLNDNKRFVERDRNWHPPAYAPGYKTTVARSPSQAYVSLQTPSVSELAGPDFTRLRMGPHDNDLLLNFRADDEARAGLP
IGERILLFGRVVDQFGKPVPNTLVEMWQANAGGRYRHKKDGYLAPLDPNFGGVGRCLSDDEGWYRFRTIKPGPYPWPNDI
NSWRPAHIHVSVMGPSISTRLITQMYFEGDPLIPLCPIVQTLRDPEAVETMVGRLDMARSRSMDCLAYRFDLVVRGELQT
YFENQG

Sequences:

>Translated_246_residues
MPLNDNKRFVERDRNWHPPAYAPGYKTTVARSPSQAYVSLQTPSVSELAGPDFTRLRMGPHDNDLLLNFRADDEARAGLP
IGERILLFGRVVDQFGKPVPNTLVEMWQANAGGRYRHKKDGYLAPLDPNFGGVGRCLSDDEGWYRFRTIKPGPYPWPNDI
NSWRPAHIHVSVMGPSISTRLITQMYFEGDPLIPLCPIVQTLRDPEAVETMVGRLDMARSRSMDCLAYRFDLVVRGELQT
YFENQG
>Mature_245_residues
PLNDNKRFVERDRNWHPPAYAPGYKTTVARSPSQAYVSLQTPSVSELAGPDFTRLRMGPHDNDLLLNFRADDEARAGLPI
GERILLFGRVVDQFGKPVPNTLVEMWQANAGGRYRHKKDGYLAPLDPNFGGVGRCLSDDEGWYRFRTIKPGPYPWPNDIN
SWRPAHIHVSVMGPSISTRLITQMYFEGDPLIPLCPIVQTLRDPEAVETMVGRLDMARSRSMDCLAYRFDLVVRGELQTY
FENQG

Specific function: Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway [H]

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000627
- InterPro:   IPR015889
- InterPro:   IPR012785 [H]

Pfam domain/function: PF00775 Dioxygenase_C [H]

EC number: =1.13.11.3 [H]

Molecular weight: Translated: 27923; Mature: 27792

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00083 INTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNDNKRFVERDRNWHPPAYAPGYKTTVARSPSQAYVSLQTPSVSELAGPDFTRLRMGP
CCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHCCCCCEEEECCC
HDNDLLLNFRADDEARAGLPIGERILLFGRVVDQFGKPVPNTLVEMWQANAGGRYRHKKD
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCC
GYLAPLDPNFGGVGRCLSDDEGWYRFRTIKPGPYPWPNDINSWRPAHIHVSVMGPSISTR
CEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCHHHH
LITQMYFEGDPLIPLCPIVQTLRDPEAVETMVGRLDMARSRSMDCLAYRFDLVVRGELQT
HHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHH
YFENQG
HHCCCC
>Mature Secondary Structure 
PLNDNKRFVERDRNWHPPAYAPGYKTTVARSPSQAYVSLQTPSVSELAGPDFTRLRMGP
CCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHCCCCCEEEECCC
HDNDLLLNFRADDEARAGLPIGERILLFGRVVDQFGKPVPNTLVEMWQANAGGRYRHKKD
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCC
GYLAPLDPNFGGVGRCLSDDEGWYRFRTIKPGPYPWPNDINSWRPAHIHVSVMGPSISTR
CEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCHHHH
LITQMYFEGDPLIPLCPIVQTLRDPEAVETMVGRLDMARSRSMDCLAYRFDLVVRGELQT
HHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHH
YFENQG
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407791; 115853; 3194022; 7990141; 9254599; 9254600; 9298971 [H]