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The map label for this gene is pcaQ [H]

Identifier: 92112444

GI number: 92112444

Start: 350102

End: 351034

Strand: Direct

Name: pcaQ [H]

Synonym: Csal_0310

Alternate gene names: 92112444

Gene position: 350102-351034 (Clockwise)

Preceding gene: 92112443

Following gene: 92112445

Centisome position: 9.47

GC content: 70.95

Gene sequence:

>933_bases
ATGCTGAGCGCTCGCGTCAAGCTGCGCCATTTGATGGCGTTTCAGGAGGTCGCGCGTCTGCAGAGCCTGGCCAAGGCGGC
GCAGGCGCTCTCCATCACCCAGCCGGCGATGTCCAAGACGATCCGCGAGCTCGAGGAGATGCTCGACGCCACGCTGTTCG
AGCGCAGCCCGCAGGGGGCCACGCTGACCGCCGCGGGACAGACGCTGCTGCGCCACGCGGGCCCGGCGCTGCGCAGCCTC
GGGGAGGGCTTTCAAGCGGTTCGCGACGCGGCTCAGCACGATACCGTGGTGCGCATGGGCGCGCTGTCGACCGTCGAGGA
AGGGCTGCTGCCGCTGGCGCTGCATCGCTTGCACCGCCGCGCGCCGGCCTTGCGGGTGCACGTCGTCACCGGCCCCAGCG
CGTACTTGCTGTCGCGCCTGCGGGCCGGCGAGCTGGACGCGGTGGCGGGGCGCATGAGCGAAGCGCGCGAAATCCGCGGC
CTGCAGTTCGAGCATCTGTACTACGAGCCGCTGGTGCTGGCCGTGCGCGTCGACCATCCCCTGCTGGCATCCAAGGCGCG
GGTCGACGAGGAGCTCGGCGCTTACCCCTGGATCGTTCCGCCGACGTCGACCACCCTGCGCGATCAGGTCGAGCGCTTCT
GGGTCGAGCGAGGCGGTACGCCGCCGCGCATCGCGCTGGAAACCCTGTCGTTGCCGCTGAGCCGTCGCTATGCCATGCGC
AGCGATGCCGTCTGGGTGGCGCCGCTGGATGCCGTGCGTGACGACATGCACGAGGGTCGCCTGCGGCGACTCCCCGTGCG
CCTCGAATCGCGCGGCGGCTCGGTGGGCCTGTGCACCAACATCGCTTTGCCGCCCAATGCCGGTGTCGAGATCCTGCGCG
AATGCCTGCACGCGAGCGTCGCCGAGCGCGCGGACAGCCCCGAGGGAACGTGA

Upstream 100 bases:

>100_bases
CGGCGGCTGGTATCCTCGATGCATCGAGGCTGACCCGGGCGAGGCGCGAGATGACGGCGCACGCGCCGGGTCCGCGCCCG
CCACCCGTGAGGACACTGCG

Downstream 100 bases:

>100_bases
CTCGGGGCCGAGAGGGCTCGTTCTTTCGTCGCATAGACCTTTGGCCGATATAGCCTTGGGGTTATGGGGGCGGGCAGCCA
TTTCAATTGGCAGCTGGGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLSARVKLRHLMAFQEVARLQSLAKAAQALSITQPAMSKTIRELEEMLDATLFERSPQGATLTAAGQTLLRHAGPALRSL
GEGFQAVRDAAQHDTVVRMGALSTVEEGLLPLALHRLHRRAPALRVHVVTGPSAYLLSRLRAGELDAVAGRMSEAREIRG
LQFEHLYYEPLVLAVRVDHPLLASKARVDEELGAYPWIVPPTSTTLRDQVERFWVERGGTPPRIALETLSLPLSRRYAMR
SDAVWVAPLDAVRDDMHEGRLRRLPVRLESRGGSVGLCTNIALPPNAGVEILRECLHASVAERADSPEGT

Sequences:

>Translated_310_residues
MLSARVKLRHLMAFQEVARLQSLAKAAQALSITQPAMSKTIRELEEMLDATLFERSPQGATLTAAGQTLLRHAGPALRSL
GEGFQAVRDAAQHDTVVRMGALSTVEEGLLPLALHRLHRRAPALRVHVVTGPSAYLLSRLRAGELDAVAGRMSEAREIRG
LQFEHLYYEPLVLAVRVDHPLLASKARVDEELGAYPWIVPPTSTTLRDQVERFWVERGGTPPRIALETLSLPLSRRYAMR
SDAVWVAPLDAVRDDMHEGRLRRLPVRLESRGGSVGLCTNIALPPNAGVEILRECLHASVAERADSPEGT
>Mature_310_residues
MLSARVKLRHLMAFQEVARLQSLAKAAQALSITQPAMSKTIRELEEMLDATLFERSPQGATLTAAGQTLLRHAGPALRSL
GEGFQAVRDAAQHDTVVRMGALSTVEEGLLPLALHRLHRRAPALRVHVVTGPSAYLLSRLRAGELDAVAGRMSEAREIRG
LQFEHLYYEPLVLAVRVDHPLLASKARVDEELGAYPWIVPPTSTTLRDQVERFWVERGGTPPRIALETLSLPLSRRYAMR
SDAVWVAPLDAVRDDMHEGRLRRLPVRLESRGGSVGLCTNIALPPNAGVEILRECLHASVAERADSPEGT

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=301, Percent_Identity=34.8837209302326, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787601, Length=267, Percent_Identity=29.9625468164794, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693105, Length=207, Percent_Identity=30.9178743961353, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789506, Length=251, Percent_Identity=26.6932270916335, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=225, Percent_Identity=28.4444444444444, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790399, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=219, Percent_Identity=26.9406392694064, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788748, Length=181, Percent_Identity=27.6243093922652, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787530, Length=270, Percent_Identity=27.7777777777778, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34090; Mature: 34090

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSARVKLRHLMAFQEVARLQSLAKAAQALSITQPAMSKTIRELEEMLDATLFERSPQGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLTAAGQTLLRHAGPALRSLGEGFQAVRDAAQHDTVVRMGALSTVEEGLLPLALHRLHRR
EEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APALRVHVVTGPSAYLLSRLRAGELDAVAGRMSEAREIRGLQFEHLYYEPLVLAVRVDHP
CCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEECCCEEEEEECCCC
LLASKARVDEELGAYPWIVPPTSTTLRDQVERFWVERGGTPPRIALETLSLPLSRRYAMR
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHC
SDAVWVAPLDAVRDDMHEGRLRRLPVRLESRGGSVGLCTNIALPPNAGVEILRECLHASV
CCCEEEEEHHHHHHHHHCCHHHHCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
AERADSPEGT
HHHCCCCCCC
>Mature Secondary Structure
MLSARVKLRHLMAFQEVARLQSLAKAAQALSITQPAMSKTIRELEEMLDATLFERSPQGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLTAAGQTLLRHAGPALRSLGEGFQAVRDAAQHDTVVRMGALSTVEEGLLPLALHRLHRR
EEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APALRVHVVTGPSAYLLSRLRAGELDAVAGRMSEAREIRGLQFEHLYYEPLVLAVRVDHP
CCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEECCCEEEEEECCCC
LLASKARVDEELGAYPWIVPPTSTTLRDQVERFWVERGGTPPRIALETLSLPLSRRYAMR
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHC
SDAVWVAPLDAVRDDMHEGRLRRLPVRLESRGGSVGLCTNIALPPNAGVEILRECLHASV
CCCEEEEEHHHHHHHHHCCHHHHCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
AERADSPEGT
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]