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The map label for this gene is cobB2 [H]

Identifier: 92112399

GI number: 92112399

Start: 302916

End: 303815

Strand: Direct

Name: cobB2 [H]

Synonym: Csal_0265

Alternate gene names: 92112399

Gene position: 302916-303815 (Clockwise)

Preceding gene: 92112397

Following gene: 92112413

Centisome position: 8.19

GC content: 69.11

Gene sequence:

>900_bases
ATGGTCGACACGTCACCGTCGCCGCTGGCGGCGTTTCTCGCACGGCATCCGCGCTTGTTCGTGCTGACCGGGGCGGGAAT
GAGCACCGATTGCGGCATTCCCGACTACCGCGATGAGCGCGGCGATTGGAAGCGCTCGCCCCCGATGACCCACCAGGCGT
TCATGCAAAGCGACCTGGCGCGCCGTCGTTACTGGGCACGCAGCCTGGTCGGGTTTCAGGCGCTCAGCGAGGCGCGGCCC
GGGCGGGGGCATTACGCGCTGGCCGAACTCGAACGGGCCGGTCGCCTCGAGCGGCTGGTCACGCAGAATGTCGACGGGCT
GCATCAGCGCGCAGGCTCGCGCCGCGTCATCGACCTGCACGGCCAGGCCGACGTGGTGCGGTGCATGGGGTGCGGCGCGA
CTCGCATGCGACATGCCTTGCACGCCGAACTGGCGCGCCTCAATCCCCACTGGACGACTCTCGAGGCGCAGGTCGGCCCG
GATGGGGATGCTGATCTGGAAAGTCGCAACTTCGCCGATTTCTCGCTGCTGTCCTGCGCGCGCTGCGGCGACGGCATCTT
CAAGCCCGATGTGGTGTTTTTCGGCGACAACGTGCCGCGTCCACGCGTGGACGCGGCATTCGCGGCGCTAGAACAGGCCG
ACGCCATGCTGGTGGCGGGCTCGTCGCTGATGGTCTATTCGGGCTATCGTTTCGCGAAGCGGGCGGCCGAACTGGGCAAG
CCGCTGGCGTGCGTCAACATGGGGCGCACTCGTGCCGATGCGTTGTTCTCGCTCAAGCTGGAGGCGCCGGTGGGCGAGAC
CCTGGAAGCGGCGGTGCGCGAGGTGACGGCGTCCCGCTCCCCGCAGACGCCGCCGCACGTCACGCAGGGCGCCGGCCCAA
TCTCGACGCTCCGCCCGTGA

Upstream 100 bases:

>100_bases
CCATTCTAGCCAGCTTGGCGGGCGGCGGCGATACGGCCCTGTGCCCGCAGGCGGCACGCGTTCTATCATAGGGTAGCGGT
TCTGGTTCTGGGAGGTCACG

Downstream 100 bases:

>100_bases
GTGCGTGCCGCGCGGGATGCCGTGTCGTCAGCGGCGCGAGGCCGGCACCAGGGTAATGGTCGGCGTGGTGCCGGTGTCGC
CGTTCAGGACCTCGACCGCG

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MVDTSPSPLAAFLARHPRLFVLTGAGMSTDCGIPDYRDERGDWKRSPPMTHQAFMQSDLARRRYWARSLVGFQALSEARP
GRGHYALAELERAGRLERLVTQNVDGLHQRAGSRRVIDLHGQADVVRCMGCGATRMRHALHAELARLNPHWTTLEAQVGP
DGDADLESRNFADFSLLSCARCGDGIFKPDVVFFGDNVPRPRVDAAFAALEQADAMLVAGSSLMVYSGYRFAKRAAELGK
PLACVNMGRTRADALFSLKLEAPVGETLEAAVREVTASRSPQTPPHVTQGAGPISTLRP

Sequences:

>Translated_299_residues
MVDTSPSPLAAFLARHPRLFVLTGAGMSTDCGIPDYRDERGDWKRSPPMTHQAFMQSDLARRRYWARSLVGFQALSEARP
GRGHYALAELERAGRLERLVTQNVDGLHQRAGSRRVIDLHGQADVVRCMGCGATRMRHALHAELARLNPHWTTLEAQVGP
DGDADLESRNFADFSLLSCARCGDGIFKPDVVFFGDNVPRPRVDAAFAALEQADAMLVAGSSLMVYSGYRFAKRAAELGK
PLACVNMGRTRADALFSLKLEAPVGETLEAAVREVTASRSPQTPPHVTQGAGPISTLRP
>Mature_299_residues
MVDTSPSPLAAFLARHPRLFVLTGAGMSTDCGIPDYRDERGDWKRSPPMTHQAFMQSDLARRRYWARSLVGFQALSEARP
GRGHYALAELERAGRLERLVTQNVDGLHQRAGSRRVIDLHGQADVVRCMGCGATRMRHALHAELARLNPHWTTLEAQVGP
DGDADLESRNFADFSLLSCARCGDGIFKPDVVFFGDNVPRPRVDAAFAALEQADAMLVAGSSLMVYSGYRFAKRAAELGK
PLACVNMGRTRADALFSLKLEAPVGETLEAAVREVTASRSPQTPPHVTQGAGPISTLRP

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=263, Percent_Identity=46.0076045627376, Blast_Score=227, Evalue=9e-60,
Organism=Homo sapiens, GI6912664, Length=273, Percent_Identity=27.8388278388278, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI13787215, Length=258, Percent_Identity=27.906976744186, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI300795542, Length=273, Percent_Identity=27.1062271062271, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI300797577, Length=262, Percent_Identity=26.3358778625954, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI300797597, Length=131, Percent_Identity=35.8778625954198, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI308199517, Length=275, Percent_Identity=29.4545454545455, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71990482, Length=275, Percent_Identity=36, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI71990487, Length=277, Percent_Identity=35.7400722021661, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17567771, Length=277, Percent_Identity=34.2960288808664, Blast_Score=177, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17541892, Length=217, Percent_Identity=28.110599078341, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571445, Length=271, Percent_Identity=43.5424354243542, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI28571443, Length=215, Percent_Identity=43.2558139534884, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28571441, Length=215, Percent_Identity=43.2558139534884, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17137536, Length=235, Percent_Identity=28.936170212766, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 32605; Mature: 32605

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDTSPSPLAAFLARHPRLFVLTGAGMSTDCGIPDYRDERGDWKRSPPMTHQAFMQSDLA
CCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RRRYWARSLVGFQALSEARPGRGHYALAELERAGRLERLVTQNVDGLHQRAGSRRVIDLH
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEC
GQADVVRCMGCGATRMRHALHAELARLNPHWTTLEAQVGPDGDADLESRNFADFSLLSCA
CCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
RCGDGIFKPDVVFFGDNVPRPRVDAAFAALEQADAMLVAGSSLMVYSGYRFAKRAAELGK
HHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCEEEEECCEEEEECCHHHHHHHHHHCC
PLACVNMGRTRADALFSLKLEAPVGETLEAAVREVTASRSPQTPPHVTQGAGPISTLRP
CEEEHHCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVDTSPSPLAAFLARHPRLFVLTGAGMSTDCGIPDYRDERGDWKRSPPMTHQAFMQSDLA
CCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RRRYWARSLVGFQALSEARPGRGHYALAELERAGRLERLVTQNVDGLHQRAGSRRVIDLH
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEC
GQADVVRCMGCGATRMRHALHAELARLNPHWTTLEAQVGPDGDADLESRNFADFSLLSCA
CCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
RCGDGIFKPDVVFFGDNVPRPRVDAAFAALEQADAMLVAGSSLMVYSGYRFAKRAAELGK
HHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCEEEEECCEEEEECCHHHHHHHHHHCC
PLACVNMGRTRADALFSLKLEAPVGETLEAAVREVTASRSPQTPPHVTQGAGPISTLRP
CEEEHHCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]