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The map label for this gene is priA [H]

Identifier: 91785728

GI number: 91785728

Start: 4797168

End: 4799411

Strand: Reverse

Name: priA [H]

Synonym: Bxe_A0045

Alternate gene names: 91785728

Gene position: 4799411-4797168 (Counterclockwise)

Preceding gene: 91785729

Following gene: 91785727

Centisome position: 98.03

GC content: 68.32

Gene sequence:

>2244_bases
GTGTTCGTCCGCGTCGCGCTGGATCATCCGCTGCCGACCTTGTTCGACTATCGGTGCGACACGCCTGAGCGGGTGGCGCC
GGGCATGCTGGTCAGCGTGCCGTTTGGTAAGCGCCACGTGGTTGGGCTTATCTGCGAAGTGACCGCTCACAGTGACGTGC
CGCCTGATCGCCTTCGCGCGATCAATAGCGTGTGCACCGCGTGTCCGCCGGTATCGTCGGAATGGTTGAGGTTGGCCGCT
TTCGCGGCGGATTACTATCAGCGTGGCCTCGGCGAAGTGGCGTTGCCGGCTTTGCCGCAAGCGCTGCGCGATGCTTCGCG
GTGGTCGCGGCTGTTTGCGCCGCAGGAGCGTTACAGCCTCACGCCCACAGGCCGAACGGCACTACCCGACGCACTGCCGG
CTCGCGCGAGCGCGTTGCGCAAGCTGGCGCAAGCGTTGGCCGAGACCGAGTTCCTGGTGGCCGCCGAGGTTCGCGCGCTG
CACCCCAAGGCGATCACGACGCTCGATGCATGGCAGGCGGAAGGCTGGGTGGCGCTGGAGCTCATCGAAGCCGCCGCCGA
GCTTGCCGCTCCGGCCTCGCCCGACGCACCGGCGCCCATGCCGACGCTGCCCACGCTCACCGACGAGCAGGCGGCGGCTG
TCGAAGCCATTCGCGACGCCAACGGTTTTGCGCCATTTCTGCTGCACGGCGTGACGGGCAGCGGCAAGACCGAGGTCTAT
CTGCGCGCACTCGCGGAAATCCTGGCCGCCAGGCCCGACGCTCAGGCGCTCGTCCTCGTGCCTGAAATCAATCTGACGCC
GCAGTTCGAAGCGGCTTTCCGCGCGCGTTTCGCCGCGCTCGAAGGCGCGTCGATCGTCACGCTGCACAGCGGTCTCGCGG
AAGGCGAGCGCGCCCGCAGCTGGTTCGCCGCTCACACAGGGCGGGCCCGCATCGTACTCGGCACCCGCCTGGCGGTGCTT
GCCTCGCTGCCGAAGCTCGCGATCATCGTCGTCGACGAGGAGCACGACCCGGCCTACAAGCAGCAGGAAGGCTTGCGCTA
TTCGGCGCGCGATCTGGCCATCTATCGCGCCAAGCAGCTCGGATTGCCGGTTGTGCTCGGCTCGGCCACACCGTCGCTGG
AAAGCTGGTGGCAAGCCGATCAGGGGCGCTACAAGCGGCTCACCCTGTCGCGCCGGGCGGTTGCCGACGCCGTATTGCCC
ACCGTCAGACTGATCGATCTGGAAGAAGAGCGGCGGCGCGGCCGGGCGTCCGTGGAAGGGTTGTCGGGGCCGTTGATCGC
CGCATTGAAGGCGCGGCTCGAACGCGGCGAGCAAAGCCTCGTGTTCCTGAACCGTCGCGGTTACGCGCCGCAGCTTGCCT
GCGACGCCTGCGGCTGGGTCGCCGGTTGTCCGCGTTGCAGCGCCTACGTGGTGCTGCATAAGCCCGAGCGCGCGCTACGC
TGCCATCACTGCGGGTGGGAAGCACGCATTCCGCGCTCGTGCCCGGAGTGCGGCAACGTCGACATCGCGCCGATGGGCCG
CGGCACGCAACGCGTCGAAGAAACGCTGGCGAGCGCGGTGCCCGGCGCGCGGGTATTGCGTATCGACGCCGACAGCACGC
GCCGCAAGGGCAGCGCACAGGCGCTGTTCTCCGACGTTCACGCGGGCGAGGTCGACATCCTCGTCGGCACGCAGATGATC
GCCAAGGGCCACGACTTCCAGCGCGTGTCGCTGGTCGGCGTGCTGAACGCCGACACCGCGCTCTTTTCCCACGACTTCCG
CGCGAGCGAGCGGCTTTTCGCGCAATTGATGCAAGTAAGCGGCCGGGCAGGCCGCGCGGGTTTGCCCGGCGAAGTGCTGG
TGCAAACGCGCTATCCGCGCCACGCGCTCTACCACGCGCTGGCGCGCCACGACTATGTCGGCTTCGCCAACTCCACGCTG
GCCGAGCGGCGCGATGCGCACTTGCCGCCGTTCGTGTATCAGGCCTTGTTGCGGGCCGAAGGCCGCACGCTCGAGGCCGC
GCTCGCGTTTCTGCAGCAGGCGGCGGCCGGCCTGGCCGGCATTCCTGCCGCCGAACGCGTGACCGTTTACGACGCCGTGC
CGCTCACCATCGTCAAGGTGATGCACGTGCACCGCGCCCAGTTGCTGATCGAGAGTGCATCGCGCGGCGCGTTGCACGCA
ACTTTGCGTGCCTGGCAGCCGCTGTTGCGCGGCATGAAGGGCGTGCTGCGCTGGAATCTGGAAGTCGATCCGCTCGACAT
CTGA

Upstream 100 bases:

>100_bases
TTTACTTTAACTTCGGGCGCTGTGCCGTCTGCTCGGCGCACACCACGAAGCGGCGTGGCGTCGCGCGCGTCGTGCTGATT
TCCGTCGACGCGTGAGTGAC

Downstream 100 bases:

>100_bases
GGCAGGTCGCCCGACCGGCGAAGCCGTTACTTCTTTGAGTCATATTCATAGAGCGAATGGTCGTTTCGTTTTGCGACGCC
GCTCGCCTATATTTCGCCGA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MFVRVALDHPLPTLFDYRCDTPERVAPGMLVSVPFGKRHVVGLICEVTAHSDVPPDRLRAINSVCTACPPVSSEWLRLAA
FAADYYQRGLGEVALPALPQALRDASRWSRLFAPQERYSLTPTGRTALPDALPARASALRKLAQALAETEFLVAAEVRAL
HPKAITTLDAWQAEGWVALELIEAAAELAAPASPDAPAPMPTLPTLTDEQAAAVEAIRDANGFAPFLLHGVTGSGKTEVY
LRALAEILAARPDAQALVLVPEINLTPQFEAAFRARFAALEGASIVTLHSGLAEGERARSWFAAHTGRARIVLGTRLAVL
ASLPKLAIIVVDEEHDPAYKQQEGLRYSARDLAIYRAKQLGLPVVLGSATPSLESWWQADQGRYKRLTLSRRAVADAVLP
TVRLIDLEEERRRGRASVEGLSGPLIAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKPERALR
CHHCGWEARIPRSCPECGNVDIAPMGRGTQRVEETLASAVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILVGTQMI
AKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARHDYVGFANSTL
AERRDAHLPPFVYQALLRAEGRTLEAALAFLQQAAAGLAGIPAAERVTVYDAVPLTIVKVMHVHRAQLLIESASRGALHA
TLRAWQPLLRGMKGVLRWNLEVDPLDI

Sequences:

>Translated_747_residues
MFVRVALDHPLPTLFDYRCDTPERVAPGMLVSVPFGKRHVVGLICEVTAHSDVPPDRLRAINSVCTACPPVSSEWLRLAA
FAADYYQRGLGEVALPALPQALRDASRWSRLFAPQERYSLTPTGRTALPDALPARASALRKLAQALAETEFLVAAEVRAL
HPKAITTLDAWQAEGWVALELIEAAAELAAPASPDAPAPMPTLPTLTDEQAAAVEAIRDANGFAPFLLHGVTGSGKTEVY
LRALAEILAARPDAQALVLVPEINLTPQFEAAFRARFAALEGASIVTLHSGLAEGERARSWFAAHTGRARIVLGTRLAVL
ASLPKLAIIVVDEEHDPAYKQQEGLRYSARDLAIYRAKQLGLPVVLGSATPSLESWWQADQGRYKRLTLSRRAVADAVLP
TVRLIDLEEERRRGRASVEGLSGPLIAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKPERALR
CHHCGWEARIPRSCPECGNVDIAPMGRGTQRVEETLASAVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILVGTQMI
AKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARHDYVGFANSTL
AERRDAHLPPFVYQALLRAEGRTLEAALAFLQQAAAGLAGIPAAERVTVYDAVPLTIVKVMHVHRAQLLIESASRGALHA
TLRAWQPLLRGMKGVLRWNLEVDPLDI
>Mature_747_residues
MFVRVALDHPLPTLFDYRCDTPERVAPGMLVSVPFGKRHVVGLICEVTAHSDVPPDRLRAINSVCTACPPVSSEWLRLAA
FAADYYQRGLGEVALPALPQALRDASRWSRLFAPQERYSLTPTGRTALPDALPARASALRKLAQALAETEFLVAAEVRAL
HPKAITTLDAWQAEGWVALELIEAAAELAAPASPDAPAPMPTLPTLTDEQAAAVEAIRDANGFAPFLLHGVTGSGKTEVY
LRALAEILAARPDAQALVLVPEINLTPQFEAAFRARFAALEGASIVTLHSGLAEGERARSWFAAHTGRARIVLGTRLAVL
ASLPKLAIIVVDEEHDPAYKQQEGLRYSARDLAIYRAKQLGLPVVLGSATPSLESWWQADQGRYKRLTLSRRAVADAVLP
TVRLIDLEEERRRGRASVEGLSGPLIAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKPERALR
CHHCGWEARIPRSCPECGNVDIAPMGRGTQRVEETLASAVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILVGTQMI
AKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALARHDYVGFANSTL
AERRDAHLPPFVYQALLRAEGRTLEAALAFLQQAAAGLAGIPAAERVTVYDAVPLTIVKVMHVHRAQLLIESASRGALHA
TLRAWQPLLRGMKGVLRWNLEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=764, Percent_Identity=40.8376963350785, Blast_Score=487, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81164; Mature: 81164

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVRVALDHPLPTLFDYRCDTPERVAPGMLVSVPFGKRHVVGLICEVTAHSDVPPDRLRA
CEEEEEECCCCCHHHCCCCCCHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCHHHHHH
INSVCTACPPVSSEWLRLAAFAADYYQRGLGEVALPALPQALRDASRWSRLFAPQERYSL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCC
TPTGRTALPDALPARASALRKLAQALAETEFLVAAEVRALHPKAITTLDAWQAEGWVALE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHCCCCCHHHH
LIEAAAELAAPASPDAPAPMPTLPTLTDEQAAAVEAIRDANGFAPFLLHGVTGSGKTEVY
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHEEECCCCCCHHHH
LRALAEILAARPDAQALVLVPEINLTPQFEAAFRARFAALEGASIVTLHSGLAEGERARS
HHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
WFAAHTGRARIVLGTRLAVLASLPKLAIIVVDEEHDPAYKQQEGLRYSARDLAIYRAKQL
HHHHCCCCEEEEECHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHC
GLPVVLGSATPSLESWWQADQGRYKRLTLSRRAVADAVLPTVRLIDLEEERRRGRASVEG
CCCEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEECHHHHHCCCCCCCC
LSGPLIAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKPERALR
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHH
CHHCGWEARIPRSCPECGNVDIAPMGRGTQRVEETLASAVPGARVLRIDADSTRRKGSAQ
HHCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
ALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVS
HHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHC
GRAGRAGLPGEVLVQTRYPRHALYHALARHDYVGFANSTLAERRDAHLPPFVYQALLRAE
CCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHC
GRTLEAALAFLQQAAAGLAGIPAAERVTVYDAVPLTIVKVMHVHRAQLLIESASRGALHA
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TLRAWQPLLRGMKGVLRWNLEVDPLDI
HHHHHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure
MFVRVALDHPLPTLFDYRCDTPERVAPGMLVSVPFGKRHVVGLICEVTAHSDVPPDRLRA
CEEEEEECCCCCHHHCCCCCCHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCHHHHHH
INSVCTACPPVSSEWLRLAAFAADYYQRGLGEVALPALPQALRDASRWSRLFAPQERYSL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCC
TPTGRTALPDALPARASALRKLAQALAETEFLVAAEVRALHPKAITTLDAWQAEGWVALE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHCCCCCHHHH
LIEAAAELAAPASPDAPAPMPTLPTLTDEQAAAVEAIRDANGFAPFLLHGVTGSGKTEVY
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHEEECCCCCCHHHH
LRALAEILAARPDAQALVLVPEINLTPQFEAAFRARFAALEGASIVTLHSGLAEGERARS
HHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
WFAAHTGRARIVLGTRLAVLASLPKLAIIVVDEEHDPAYKQQEGLRYSARDLAIYRAKQL
HHHHCCCCEEEEECHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHC
GLPVVLGSATPSLESWWQADQGRYKRLTLSRRAVADAVLPTVRLIDLEEERRRGRASVEG
CCCEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEECHHHHHCCCCCCCC
LSGPLIAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKPERALR
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHH
CHHCGWEARIPRSCPECGNVDIAPMGRGTQRVEETLASAVPGARVLRIDADSTRRKGSAQ
HHCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
ALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVS
HHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHC
GRAGRAGLPGEVLVQTRYPRHALYHALARHDYVGFANSTLAERRDAHLPPFVYQALLRAE
CCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHC
GRTLEAALAFLQQAAAGLAGIPAAERVTVYDAVPLTIVKVMHVHRAQLLIESASRGALHA
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TLRAWQPLLRGMKGVLRWNLEVDPLDI
HHHHHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]