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The map label for this gene is alkJ [H]

Identifier: 91785603

GI number: 91785603

Start: 4661679

End: 4663337

Strand: Reverse

Name: alkJ [H]

Synonym: Bxe_A0174

Alternate gene names: 91785603

Gene position: 4663337-4661679 (Counterclockwise)

Preceding gene: 91785606

Following gene: 91785602

Centisome position: 95.25

GC content: 67.09

Gene sequence:

>1659_bases
ATGCAATACGACTACATCATCGTCGGCGCAGGCTCGGGTGGCTGTTCGCTGGCGAGCCGTCTCGCGGACAACTGCCCGGA
CGCGACGATTGCGCTGATCGAAGCCGGGCCGCACACGGACCGCAATCTGTTCGTCAACATGCCGGTCGGCGTGGCGGCCG
TGGTGCCGCACAAGCTCAAAACCAACTACGGATATCTGACCACGCCGCAGCCGGGACTCGGCGGGCGGCAAGGGTATCAG
CCGCGCGGACGTGGCTTCGGCGGATCGAGCGCGATCAACGCGATGATCTACACGCGCGGCCATCCGCTCGATTACGACGA
ATGGGCTGAACTGGGTTGCGAAGGCTGGTCGTGGGCGGAGGTGCTGCCGTACTTTCGCCGCGCCGAAGGCAACGAACGCG
GTGCCGACGCCTGGCATGGCGATAGCGGCCCGCTCACCGTCTCCGATCTGCGCTATCAGAATCCGTTTTCGCGCCGTTTC
GTGCAGGCGGCGATGGAAGCCGGCTACAAGCCGAATAGCGACTTCAACGGTGCGGAGCAGGAAGGGATCGGCTTCTATCA
GGTGACGCAGCGCGACGGGCGCCGTTGCAGCGTGGCGCGCGCCTATATCTACGACCGCGCGCGCCCCAATCTGCACACGA
TCGCCGACGCGACGGTGCTGCGCGTGGCCTTCAAAGGCAAGCGCGCGAGCGGCGTGGAGATCGTGCGCGGCGGCCGCCGG
GAAACGCTCGAAGCGCGCGCCGAGGTGGTGCTCGCCGCGGGCGCGTTCAATTCGCCGCAACTGCTGATGTGCTCGGGCAT
CGGGCCGGCGGCGCACTTGCGCTCGTTCGGCATTCCGGTGCTGCACGACGCGCCGGAGGTCGGGCAGAACCTGATCGACC
ACGTCGATTTCACGATCAACAAACGGGTTTCGTCGATCGAGCCGACCGGCTTTTCGATTCGCGGCATTGCGCGGATGCTG
CCGCAGTTCGTTACTTTCATGCGCCACGGGCGCGGCATGCTGTCGAGCAATGTCGCCGAAGCGGGCGGTTTTCTGAAGAG
CAGCCCGACGCTCGAGCGGCCGGATCTGCAGTTGCACTTCTGCGCGGCGATCGTCGACGACCACAACCGCCACATGCATT
GGGGCCACGGTTATTCGCTGCACGTGTGCGTTTTGCGACCGCATAGCCGTGGCACCGTGACGCTGGCGAGTGCCGATGCG
CGCACCGCGCCCGTGATCGATCCGCGCTTTTTCAGCGACTCGCGCGATCTCGACCTGCTGGTGGAAGGCGCGCAGATGGC
GCGGCGCATCCTCGATGCGCCTTCGCTCGCGCTGCACGGCGGCCGCGAGTTGTACACGCATCCCGGCCAGTCGGAGGCGG
AGTTGCGCCGGACCATTGCCGAGCATGCCGACACGATCTACCACCCGGTGGCAACGTGCCGGATGGGGGGCGACGTGCGC
TCGGTGGTCGATCCGCAACTGCGGGTGCGGGGCGTCACGGGGCTGCGGATCGTGGATGCTTCGGTGATGCCGACGCTGAT
CGGCGGTAACACCAATTCGCCGACGGTGATGATCGGCGAGCGGGCCGCCGAGCTGATCGCGGCGGCGTGGCGCGGCGCGT
CGGCGGAGCCGGCAGCGGCACCGAACCACGGAACCCGCACGGCGACGAATGCCGTTTGA

Upstream 100 bases:

>100_bases
CTCTGGCACGAACCCGCCGCGGTATCCGAACGCTCCCTACAATGCCGGAAAACATACGGAGACAGGGTTCGCGAACAACG
CGGCCCACAAGGAGGAGCGG

Downstream 100 bases:

>100_bases
GCTTCGCCAGCATTGCAGATTTGTCCGGCAAATATGAGACAACTTGGCCGAACGTCGGTCAATTCGTCATAAATTGTGCC
GCTTCGTTTATGAGATTCCC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MQYDYIIVGAGSGGCSLASRLADNCPDATIALIEAGPHTDRNLFVNMPVGVAAVVPHKLKTNYGYLTTPQPGLGGRQGYQ
PRGRGFGGSSAINAMIYTRGHPLDYDEWAELGCEGWSWAEVLPYFRRAEGNERGADAWHGDSGPLTVSDLRYQNPFSRRF
VQAAMEAGYKPNSDFNGAEQEGIGFYQVTQRDGRRCSVARAYIYDRARPNLHTIADATVLRVAFKGKRASGVEIVRGGRR
ETLEARAEVVLAAGAFNSPQLLMCSGIGPAAHLRSFGIPVLHDAPEVGQNLIDHVDFTINKRVSSIEPTGFSIRGIARML
PQFVTFMRHGRGMLSSNVAEAGGFLKSSPTLERPDLQLHFCAAIVDDHNRHMHWGHGYSLHVCVLRPHSRGTVTLASADA
RTAPVIDPRFFSDSRDLDLLVEGAQMARRILDAPSLALHGGRELYTHPGQSEAELRRTIAEHADTIYHPVATCRMGGDVR
SVVDPQLRVRGVTGLRIVDASVMPTLIGGNTNSPTVMIGERAAELIAAAWRGASAEPAAAPNHGTRTATNAV

Sequences:

>Translated_552_residues
MQYDYIIVGAGSGGCSLASRLADNCPDATIALIEAGPHTDRNLFVNMPVGVAAVVPHKLKTNYGYLTTPQPGLGGRQGYQ
PRGRGFGGSSAINAMIYTRGHPLDYDEWAELGCEGWSWAEVLPYFRRAEGNERGADAWHGDSGPLTVSDLRYQNPFSRRF
VQAAMEAGYKPNSDFNGAEQEGIGFYQVTQRDGRRCSVARAYIYDRARPNLHTIADATVLRVAFKGKRASGVEIVRGGRR
ETLEARAEVVLAAGAFNSPQLLMCSGIGPAAHLRSFGIPVLHDAPEVGQNLIDHVDFTINKRVSSIEPTGFSIRGIARML
PQFVTFMRHGRGMLSSNVAEAGGFLKSSPTLERPDLQLHFCAAIVDDHNRHMHWGHGYSLHVCVLRPHSRGTVTLASADA
RTAPVIDPRFFSDSRDLDLLVEGAQMARRILDAPSLALHGGRELYTHPGQSEAELRRTIAEHADTIYHPVATCRMGGDVR
SVVDPQLRVRGVTGLRIVDASVMPTLIGGNTNSPTVMIGERAAELIAAAWRGASAEPAAAPNHGTRTATNAV
>Mature_552_residues
MQYDYIIVGAGSGGCSLASRLADNCPDATIALIEAGPHTDRNLFVNMPVGVAAVVPHKLKTNYGYLTTPQPGLGGRQGYQ
PRGRGFGGSSAINAMIYTRGHPLDYDEWAELGCEGWSWAEVLPYFRRAEGNERGADAWHGDSGPLTVSDLRYQNPFSRRF
VQAAMEAGYKPNSDFNGAEQEGIGFYQVTQRDGRRCSVARAYIYDRARPNLHTIADATVLRVAFKGKRASGVEIVRGGRR
ETLEARAEVVLAAGAFNSPQLLMCSGIGPAAHLRSFGIPVLHDAPEVGQNLIDHVDFTINKRVSSIEPTGFSIRGIARML
PQFVTFMRHGRGMLSSNVAEAGGFLKSSPTLERPDLQLHFCAAIVDDHNRHMHWGHGYSLHVCVLRPHSRGTVTLASADA
RTAPVIDPRFFSDSRDLDLLVEGAQMARRILDAPSLALHGGRELYTHPGQSEAELRRTIAEHADTIYHPVATCRMGGDVR
SVVDPQLRVRGVTGLRIVDASVMPTLIGGNTNSPTVMIGERAAELIAAAWRGASAEPAAAPNHGTRTATNAV

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=540, Percent_Identity=41.1111111111111, Blast_Score=384, Evalue=1e-106,
Organism=Escherichia coli, GI1786503, Length=550, Percent_Identity=38.7272727272727, Blast_Score=347, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17532301, Length=546, Percent_Identity=37.7289377289377, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI45549471, Length=579, Percent_Identity=35.9240069084629, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI45551458, Length=579, Percent_Identity=35.9240069084629, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24642042, Length=578, Percent_Identity=37.0242214532872, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24642055, Length=582, Percent_Identity=35.0515463917526, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24642048, Length=596, Percent_Identity=34.0604026845638, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24642037, Length=575, Percent_Identity=36.695652173913, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24650267, Length=574, Percent_Identity=36.411149825784, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24642035, Length=576, Percent_Identity=35.4166666666667, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24642059, Length=577, Percent_Identity=33.2755632582322, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24642039, Length=573, Percent_Identity=34.9040139616056, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI18859995, Length=570, Percent_Identity=34.3859649122807, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI18859993, Length=586, Percent_Identity=32.7645051194539, Blast_Score=265, Evalue=8e-71,
Organism=Drosophila melanogaster, GI17137792, Length=562, Percent_Identity=32.9181494661922, Blast_Score=262, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24642051, Length=570, Percent_Identity=29.2982456140351, Blast_Score=232, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24645930, Length=579, Percent_Identity=28.6701208981002, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24642057, Length=587, Percent_Identity=28.9608177172061, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59758; Mature: 59758

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYDYIIVGAGSGGCSLASRLADNCPDATIALIEAGPHTDRNLFVNMPVGVAAVVPHKLK
CCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCEEECCCHHHC
TNYGYLTTPQPGLGGRQGYQPRGRGFGGSSAINAMIYTRGHPLDYDEWAELGCEGWSWAE
CCCCEEECCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNERGADAWHGDSGPLTVSDLRYQNPFSRRFVQAAMEAGYKPNSDFNGAEQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
EGIGFYQVTQRDGRRCSVARAYIYDRARPNLHTIADATVLRVAFKGKRASGVEIVRGGRR
CCCCEEEEECCCCCEEHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCCHHHHCCCCH
ETLEARAEVVLAAGAFNSPQLLMCSGIGPAAHLRSFGIPVLHDAPEVGQNLIDHVDFTIN
HHHHHHEEEEEEECCCCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEC
KRVSSIEPTGFSIRGIARMLPQFVTFMRHGRGMLSSNVAEAGGFLKSSPTLERPDLQLHF
CHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCCCCCCCCCEEEE
CAAIVDDHNRHMHWGHGYSLHVCVLRPHSRGTVTLASADARTAPVIDPRFFSDSRDLDLL
EEEECCCCCCEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHEE
VEGAQMARRILDAPSLALHGGRELYTHPGQSEAELRRTIAEHADTIYHPVATCRMGGDVR
EHHHHHHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVVDPQLRVRGVTGLRIVDASVMPTLIGGNTNSPTVMIGERAAELIAAAWRGASAEPAAA
HHCCCCCEEECCCCEEEECCCCCCEEECCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCC
PNHGTRTATNAV
CCCCCCCCCCCC
>Mature Secondary Structure
MQYDYIIVGAGSGGCSLASRLADNCPDATIALIEAGPHTDRNLFVNMPVGVAAVVPHKLK
CCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCEEECCCHHHC
TNYGYLTTPQPGLGGRQGYQPRGRGFGGSSAINAMIYTRGHPLDYDEWAELGCEGWSWAE
CCCCEEECCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNERGADAWHGDSGPLTVSDLRYQNPFSRRFVQAAMEAGYKPNSDFNGAEQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
EGIGFYQVTQRDGRRCSVARAYIYDRARPNLHTIADATVLRVAFKGKRASGVEIVRGGRR
CCCCEEEEECCCCCEEHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCCHHHHCCCCH
ETLEARAEVVLAAGAFNSPQLLMCSGIGPAAHLRSFGIPVLHDAPEVGQNLIDHVDFTIN
HHHHHHEEEEEEECCCCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEC
KRVSSIEPTGFSIRGIARMLPQFVTFMRHGRGMLSSNVAEAGGFLKSSPTLERPDLQLHF
CHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCCCCCCCCCEEEE
CAAIVDDHNRHMHWGHGYSLHVCVLRPHSRGTVTLASADARTAPVIDPRFFSDSRDLDLL
EEEECCCCCCEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHEE
VEGAQMARRILDAPSLALHGGRELYTHPGQSEAELRRTIAEHADTIYHPVATCRMGGDVR
EHHHHHHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVVDPQLRVRGVTGLRIVDASVMPTLIGGNTNSPTVMIGERAAELIAAAWRGASAEPAAA
HHCCCCCEEECCCCEEEECCCCCCEEECCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCC
PNHGTRTATNAV
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]