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The map label for this gene is hisH [H]

Identifier: 91785377

GI number: 91785377

Start: 4435284

End: 4435925

Strand: Reverse

Name: hisH [H]

Synonym: Bxe_A0402

Alternate gene names: 91785377

Gene position: 4435925-4435284 (Counterclockwise)

Preceding gene: 91785378

Following gene: 91785376

Centisome position: 90.61

GC content: 64.8

Gene sequence:

>642_bases
ATGAAAACTTCGATAGCGATTGTGGATTACGGAATGGGCAACCTGCGTTCGGTTGCCCAGGCGCTGCGCAAAGCCGCGCC
GGAAGCGGATGTGGCGATCGTCGACCGGCCGGAAGCGATCCACGCCGCCGGCCGCGTGGTGCTGCCCGGCCAGGGCGCGA
TGCCCGACTGCATGCGCAGTCTCGGCGAGTCCGGCCTGCAGGAAGCGGTGATCGAAGCATCCCGCACCAAGCCGCTGATG
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCAACACGCCGGGTCTCGGTCTACTGCCCGGCAA
GGTGCTGCGCTTCGATCTGGAAGGCCAGTTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAAATGGGCTGGAACCGCG
TGCATCAGACCCGGCCGCATCCGCTGTGGGACGGTGTCGCGGACAACGCGTTCTTCTACTTCGTGCACAGTTACTACGTG
GTGCCCGATAATGCGGCCCATACCGTGGGCGAAACGGTGTACGGCGTGCCCTTTACCTCGGCGGTGGCGCGCGATAACAT
CTTCGCGACCCAATTCCACCCGGAAAAGAGCGCCGAAGCGGGGCTGCGCGTGTATCGCAACTTCGTGCACTGGAACCCGT
GA

Upstream 100 bases:

>100_bases
CGGGTATCAACATCGGCACGCGGCTCATGGGTTTGATGTTATCGGCGCTGGCGGTGGAATTCATCGTCGATGGATTGAAG
GCATTGCTGCCTAATTTGAA

Downstream 100 bases:

>100_bases
GCGCCTTTCTTCATCCTGTTGCCGTGCGTCGTTCTGCCACAAGCGGAACGATCGCCGCGCGCCGCACCCGCAGCACCGCG
ATTTCGCTTGCGCCCGACGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALRKAAPEADVAIVDRPEAIHAAGRVVLPGQGAMPDCMRSLGESGLQEAVIEASRTKPLM
GVCVGEQMLFDWSAEGNTPGLGLLPGKVLRFDLEGQLQDDGSRFKVPQMGWNRVHQTRPHPLWDGVADNAFFYFVHSYYV
VPDNAAHTVGETVYGVPFTSAVARDNIFATQFHPEKSAEAGLRVYRNFVHWNP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALRKAAPEADVAIVDRPEAIHAAGRVVLPGQGAMPDCMRSLGESGLQEAVIEASRTKPLM
GVCVGEQMLFDWSAEGNTPGLGLLPGKVLRFDLEGQLQDDGSRFKVPQMGWNRVHQTRPHPLWDGVADNAFFYFVHSYYV
VPDNAAHTVGETVYGVPFTSAVARDNIFATQFHPEKSAEAGLRVYRNFVHWNP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALRKAAPEADVAIVDRPEAIHAAGRVVLPGQGAMPDCMRSLGESGLQEAVIEASRTKPLM
GVCVGEQMLFDWSAEGNTPGLGLLPGKVLRFDLEGQLQDDGSRFKVPQMGWNRVHQTRPHPLWDGVADNAFFYFVHSYYV
VPDNAAHTVGETVYGVPFTSAVARDNIFATQFHPEKSAEAGLRVYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=207, Percent_Identity=36.231884057971, Blast_Score=120, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=33.9449541284404, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23338; Mature: 23338

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALRKAAPEADVAIVDRPEAIHAAGRVVLPGQGAMPDCMRS
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVIEASRTKPLMGVCVGEQMLFDWSAEGNTPGLGLLPGKVLRFDLEGQLQDD
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCC
GSRFKVPQMGWNRVHQTRPHPLWDGVADNAFFYFVHSYYVVPDNAAHTVGETVYGVPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHCCEEECCCCHH
AVARDNIFATQFHPEKSAEAGLRVYRNFVHWNP
HHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALRKAAPEADVAIVDRPEAIHAAGRVVLPGQGAMPDCMRS
CCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVIEASRTKPLMGVCVGEQMLFDWSAEGNTPGLGLLPGKVLRFDLEGQLQDD
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCC
GSRFKVPQMGWNRVHQTRPHPLWDGVADNAFFYFVHSYYVVPDNAAHTVGETVYGVPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHCCEEECCCCHH
AVARDNIFATQFHPEKSAEAGLRVYRNFVHWNP
HHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]