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The map label for this gene is flhA [H]

Identifier: 91785067

GI number: 91785067

Start: 4092002

End: 4093108

Strand: Reverse

Name: flhA [H]

Synonym: Bxe_A0713

Alternate gene names: 91785067

Gene position: 4093108-4092002 (Counterclockwise)

Preceding gene: 91785068

Following gene: 91785066

Centisome position: 83.6

GC content: 61.79

Gene sequence:

>1107_bases
ATGAAGACCAAAGCAGCAATCGCATGGAAAGCCGGCGCCCCGTTGACGATCGAGGAAGTCGATCTGGAAGGTCCGCGGGC
CGGTGAAGTCCTGATCGAAGTGAAGGCCACGGGCATCTGCCATACCGATTACTACACGCTCTCCGGCGCGGATCCGGAAG
GCATTTTCCCGGCGATTCTCGGCCACGAAGGCGCGGGCGTGATCGTCGACACCGGGCCGGGTGTCGGCACGCTGAAGAAG
GGCGACCACGTCATTCCGCTCTACACGCCGGAATGCCGGCAGTGCAAGTTCTGCCTGTCGCGCAAGACCAATCTCTGTCA
GGCAATCCGATCGACGCAGGGCAAGGGTCTGATGCCCGACTCGACCTCGCGTTTCTCGATCGACGGCAAGCCTTTGTTCC
ACTACATGGGCACGTCCACGTTTTCGAACTACATCGTCGTGCCGGAAATCGCGGTCGCCAAGGTGCGTGAAGACGCGCCC
TTCGACAAGATCTGCTACATCGGCTGCGGTGTGACGACGGGCGTGGGCGCGGTCGTGTATTCGGCCAAGGTCGAGGCCGG
CGCGAACGTGGTGGTGTTCGGTCTCGGCGGCATCGGCCTGAACGTGATCCAGGGTGCCAAAATGGTCGGCGCGGACAGGA
TCATCGGTGTGGACATCAATTCGGGCCGCGTCGAACTCGCGAAGAAGTTCGGCATGACGCACTTCATCAACCCGACCGAA
GTGGAGAACGTGGTCGATCACATCGTGCAACTCACTGATGGCGGCGCGGACTATTCGTTCGAGTGCATCGGCAACACCAA
GGTGATGCGTCAGGCGCTGGAGTGCACGCACAAGGGCTGGGGCCAGTCGTTCATCATCGGCGTGGCGGCGGCGGGCGAGG
AGATCAGCACGCGTCCGTTCCAGCTGGTGACGGGCCGCGAATGGAAAGGCTCGGCGTTCGGCGGCGCGCGCGGACGCACG
GACGTGCCGAAGATCGTCGACTGGTACATGGAAGGCAAGATCAATATCGACGACCTGATCACGCACCGTCTGCCGCTCGA
ACGCATCAACGAAGGCTTCGATCTGATGAAGAAGGGCGAGTCGATCCGCTCGGTCGTGCTGTACTAA

Upstream 100 bases:

>100_bases
CACACGCATCAACCGCAGTACCGAATCGCAGCACGTGCGCGCCGCGGGGTTCCGGGCACGCAACGCGCCTTGCATTGACA
GACTCACAGGAGCATTCACG

Downstream 100 bases:

>100_bases
TCCAATGCGGCACAACGAGGAGCATGGCGATGCTTGAACTTCTGTCGTCGCACGCCTGTCACGGCGGCGAGCAGCGCATC
TATCGGCACGACTCGCAGAC

Product: glutathione-dependent formaldehyde dehydrogenase(FlhA)

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVIVDTGPGVGTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRSTQGKGLMPDSTSRFSIDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADRIIGVDINSGRVELAKKFGMTHFINPTE
VENVVDHIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHRLPLERINEGFDLMKKGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVIVDTGPGVGTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRSTQGKGLMPDSTSRFSIDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADRIIGVDINSGRVELAKKFGMTHFINPTE
VENVVDHIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHRLPLERINEGFDLMKKGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVIVDTGPGVGTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRSTQGKGLMPDSTSRFSIDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADRIIGVDINSGRVELAKKFGMTHFINPTE
VENVVDHIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHRLPLERINEGFDLMKKGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=60.7629427792915, Blast_Score=453, Evalue=1e-128,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.0588235294118, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=45.7994579945799, Blast_Score=347, Evalue=7e-96,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.5284552845528, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.2574525745258, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=44.3243243243243, Blast_Score=334, Evalue=7e-92,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=44.3243243243243, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=43.75, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=43.7673130193906, Blast_Score=322, Evalue=4e-88,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=57.7235772357724, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI87082125, Length=379, Percent_Identity=27.4406332453826, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=27.3936170212766, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788073, Length=364, Percent_Identity=25.2747252747253, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786825, Length=382, Percent_Identity=25.3926701570681, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI226510992, Length=372, Percent_Identity=25.8064516129032, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=28.6549707602339, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787863, Length=349, Percent_Identity=24.9283667621776, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788075, Length=382, Percent_Identity=28.0104712041885, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.5231607629428, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=53.887399463807, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=53.6, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=54.5994065281899, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=28.0653950953678, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17562876, Length=356, Percent_Identity=27.5280898876405, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562582, Length=330, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=25.3369272237197, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562878, Length=356, Percent_Identity=23.5955056179775, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=53.3692722371968, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6324486, Length=371, Percent_Identity=26.9541778975741, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323729, Length=364, Percent_Identity=28.021978021978, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=372, Percent_Identity=27.6881720430108, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=28.1501340482574, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.9230769230769, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=365, Percent_Identity=28.7671232876712, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=365, Percent_Identity=28.7671232876712, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=367, Percent_Identity=26.158038147139, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=257, Percent_Identity=27.6264591439689, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=58.9673913043478, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17137530, Length=343, Percent_Identity=28.2798833819242, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39559; Mature: 39559

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVIVDTGPGVGTLKKGDHVIPLYTPECRQCKFCLSRKTNLCQAIRSTQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
STSRFSIDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCEEEECCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADRIIGVDINSGRVELAKKFGMTHFINPTE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEEECCCCHHHHHHHCCCEECCCHHH
VENVVDHIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHRLPLERINEGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
CHHEEECC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVIVDTGPGVGTLKKGDHVIPLYTPECRQCKFCLSRKTNLCQAIRSTQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
STSRFSIDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCEEEECCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADRIIGVDINSGRVELAKKFGMTHFINPTE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEEECCCCHHHHHHHCCCEECCCHHH
VENVVDHIVQLTDGGADYSFECIGNTKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHRLPLERINEGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
CHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]