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The map label for this gene is kefA [H]

Identifier: 91784969

GI number: 91784969

Start: 3979953

End: 3981308

Strand: Reverse

Name: kefA [H]

Synonym: Bxe_A0811

Alternate gene names: 91784969

Gene position: 3981308-3979953 (Counterclockwise)

Preceding gene: 91784970

Following gene: 91784968

Centisome position: 81.32

GC content: 63.94

Gene sequence:

>1356_bases
ATGCAAAACCGTCTTTTCCCTCATATGTTCAGCGACCTCGCGCGCGACTTCGGTCAGCCGGTGATGCTGTGGCAAGTCGG
CGTCCTGCTCGGGACGCTCGCGATCGCGTGGCTGCTCGCGCGCCTGTTGCGCCGGGCACTGGATCTGCGTCGCCAGACGC
GTTACCAGACTCTGCGCTTCGGCGCGGAAAGCCTGAACCGCGCGTTCTTTCCGCTGATCGGCGCGACCCTCGTGTGGCTC
GCGCGGTCGGTCACCGGCCAGTTCATGCACACCGCGCTGCTCGATCTGGCGCTGGTGCCGTTATTCGGCATCGGCTTGAT
TTACATTGTGTTTTTCTTTGCGCGGCGGGTTTTCAGCCACGACGGCGCGACGCATCCGTGGCTGTTCCTCGTCGAAAAGA
TCGTCTCGCTGGTCGTCTGGGTCGGCATGGTGCTCACGGTCATGGGCATTCAGGACGACGTGATCACGTGGATGGCGAGC
GTCCAGTTCCGTGTCGCCAACGCGCACATGACGCTGCTCTCGCTCATCTCGGGGCTGCTGTGGGTGTGCGTGACGATGAT
CGTCGCGATGTGGCTCGGCTCCACGTTCGAAGACCGTCTGATGCGTTCGAAAACGCTCGATGCCAACCTGAAGGTGGTGG
TGGCGCGGGTCGGCCGGGCGGCGTTCGTGCTGGCGGCGGTGCTGATCAGCCTGTCGCTGGTCGGCATCGACATCACCGTG
CTGGGTGTGTTCGGCGGCGCGCTGGGCGTGGGGCTGGGGTTCGGTCTGCAGAAGATCGCCAGCAACTACGTGTCGGGATT
CATCATCCTGATCGACCGGTCGCTGCGCATTGGCGACACGATCAACGTGAGCGGCCTGCAGGGCATGGTCACGCAGATCC
GCACGCGCTATACCGTGGTGCGTGGACTGGACGGCATCGAGACGCTGATTCCGAACGAAAAGCTGATCACGGACGTGGTC
CAGAACCAGTCGTCGTACCTGACGCGCGGCCATGCGAAAGTGGCCGTGCAGGTCGCGTACACGTCCAACGTCGAGCAGGC
AATGGCGCTGCTGGTGCAGGCCGCCGCAGACGTGCCGCGCGTGCTGCAGGAACCGGCGCCGACATCTTATCTGGCCAGTT
TCGGTGCGGACGGCATCAACCTCGAACTCGGCTTCTGGATCGAGGACGCGGCCGCCGGCACCGCGGGCGTGCGCTCGGCC
GTCAACCGCAATATCTGGCGTCTGTTCTCGGAGCACGACATCTCGATTCCGTTCGCGCAACGCGAAGTGCGAATCGTCGG
CAACGTTGGCGTCGGTGTACCGCAACCGGTAATAATGACCGGATCCTCGGCCGATCAGGCGGACAGCGCGCATTGA

Upstream 100 bases:

>100_bases
GCTGGAACGCCAGAGCTAGGCGCAGGCGTAACAGGCTTCTGCCGGCGCGCGCGGGTTCGATTCCTGAACCCGGCTGGGCG
CCGGCGACTTCCGCGACATC

Downstream 100 bases:

>100_bases
CGGGCACTTCTGGCGCGCCGGCGAGTCTTATATAGAGCTTCGGCGCGTTCCTTTCCGTTGATGCGGCACAAAAAATCCCT
ATTTGTTACAAACACTTGGA

Product: small-conductance mechanosensitive channel protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQNRLFPHMFSDLARDFGQPVMLWQVGVLLGTLAIAWLLARLLRRALDLRRQTRYQTLRFGAESLNRAFFPLIGATLVWL
ARSVTGQFMHTALLDLALVPLFGIGLIYIVFFFARRVFSHDGATHPWLFLVEKIVSLVVWVGMVLTVMGIQDDVITWMAS
VQFRVANAHMTLLSLISGLLWVCVTMIVAMWLGSTFEDRLMRSKTLDANLKVVVARVGRAAFVLAAVLISLSLVGIDITV
LGVFGGALGVGLGFGLQKIASNYVSGFIILIDRSLRIGDTINVSGLQGMVTQIRTRYTVVRGLDGIETLIPNEKLITDVV
QNQSSYLTRGHAKVAVQVAYTSNVEQAMALLVQAAADVPRVLQEPAPTSYLASFGADGINLELGFWIEDAAAGTAGVRSA
VNRNIWRLFSEHDISIPFAQREVRIVGNVGVGVPQPVIMTGSSADQADSAH

Sequences:

>Translated_451_residues
MQNRLFPHMFSDLARDFGQPVMLWQVGVLLGTLAIAWLLARLLRRALDLRRQTRYQTLRFGAESLNRAFFPLIGATLVWL
ARSVTGQFMHTALLDLALVPLFGIGLIYIVFFFARRVFSHDGATHPWLFLVEKIVSLVVWVGMVLTVMGIQDDVITWMAS
VQFRVANAHMTLLSLISGLLWVCVTMIVAMWLGSTFEDRLMRSKTLDANLKVVVARVGRAAFVLAAVLISLSLVGIDITV
LGVFGGALGVGLGFGLQKIASNYVSGFIILIDRSLRIGDTINVSGLQGMVTQIRTRYTVVRGLDGIETLIPNEKLITDVV
QNQSSYLTRGHAKVAVQVAYTSNVEQAMALLVQAAADVPRVLQEPAPTSYLASFGADGINLELGFWIEDAAAGTAGVRSA
VNRNIWRLFSEHDISIPFAQREVRIVGNVGVGVPQPVIMTGSSADQADSAH
>Mature_451_residues
MQNRLFPHMFSDLARDFGQPVMLWQVGVLLGTLAIAWLLARLLRRALDLRRQTRYQTLRFGAESLNRAFFPLIGATLVWL
ARSVTGQFMHTALLDLALVPLFGIGLIYIVFFFARRVFSHDGATHPWLFLVEKIVSLVVWVGMVLTVMGIQDDVITWMAS
VQFRVANAHMTLLSLISGLLWVCVTMIVAMWLGSTFEDRLMRSKTLDANLKVVVARVGRAAFVLAAVLISLSLVGIDITV
LGVFGGALGVGLGFGLQKIASNYVSGFIILIDRSLRIGDTINVSGLQGMVTQIRTRYTVVRGLDGIETLIPNEKLITDVV
QNQSSYLTRGHAKVAVQVAYTSNVEQAMALLVQAAADVPRVLQEPAPTSYLASFGADGINLELGFWIEDAAAGTAGVRSA
VNRNIWRLFSEHDISIPFAQREVRIVGNVGVGVPQPVIMTGSSADQADSAH

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=202, Percent_Identity=37.1287128712871, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI2367355, Length=299, Percent_Identity=27.7591973244147, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789291, Length=263, Percent_Identity=27.3764258555133, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 49291; Mature: 49291

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEEECCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure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CEEEEEECCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]