Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 91784748

GI number: 91784748

Start: 3734734

End: 3736860

Strand: Reverse

Name: pcrA [H]

Synonym: Bxe_A1045

Alternate gene names: 91784748

Gene position: 3736860-3734734 (Counterclockwise)

Preceding gene: 91784749

Following gene: 91784741

Centisome position: 76.33

GC content: 66.71

Gene sequence:

>2127_bases
GTGCTTCCCGTCGCCGAATCCCCGCCGTTCGATACCGCCGCCTGGCTGGCCAGGCTCAACGACGCGCAGCGCGAAGCCGT
CGAATATGGCGCCGATACGCCCACGGCGCCGCCCGGCGCGCTGCTGGTGATCGCGGGCGCCGGCTCGGGCAAGACCAACA
CGCTCGCACACCGGGTCGCCAATCTGGTCGTGAACGGCGCCGATCCGCGCCGCATCCTGTTGCTGACCTTTTCGCGCCGC
GCGGCGCTCGAAATGATTCGCCGCGTCACGCGCATCGCCGGCGCGGCGCTCGGCACGCGAGCCGCGCTCGCGCAAGGGCT
GACGTGGTCGGGCACGTTCCATAGTGTCGGCGCGCGGCTGTTGCGCGAGTACGCGGACCTGATCGGCCTCGCGCCGGCCT
TCACGATCAACGACCGCGAGGATTCCGCCGACCTGATGAACCTCGTGCGCCACGAACTCGGTTTGTCGGCGAAAGAGCGG
CGTTTCCCGGCCAAGGGCACGTGTTTTGCCATTTACTCGCGCGTCGTGAACACCGGCGCGTCGCTCAGCCAGGTGCTCGA
CAAGGCGTTTCCCTGGTGCCGTGAATGGGAGGCGGATCTGCGCATGCTGTTCGCCGCTTACGTCGAGGCGAAGCAGAAAC
AGAGCGTGCTCGATTACGACGACCTGCTGCTTTACTGGTCGCTTATGGCCGCCGAGCCGGCCATCGCCGCTGACCTGTCA
GCGCGTTTCGACCACGTGCTGGTGGACGAATATCAGGACACCAACCGGCTGCAGGCGTCGATCCTGCTCGCGCTGAAGCC
AGACGGCCGCGGCCTGACCGTGGTCGGCGACGACGCCCAGTCGATCTATTCGTTTCGCGGCGCCACCGTGCGCAACATTC
TCGATTTTCCCGCGCACTTCGATCCACCGGCCAGACAGGTCACGCTCGAACGCAATTACCGGTCCACCGCGCCGATTCTG
GCTGCGTCGAATGCGGTGATCGAACTGGCCAGCGAGCGCTACACGAAGAATCTGTGGACCGACAAGGCGTCGGCACAACG
CCCGCGTCTGGTGAGCGTCGCCGACGAGGCCGATCAGGCGCGCTACGTCGTCGAACAGGTGCTCGAGGCGCGCGAGCAGG
GCATGAAGCTCAAGTCGCAGGCGGTGCTGTTTCGCGCCGCGCATCACAGCGCCGCGCTGGAGATCGAGCTGACCCGGCGC
AACATTCCGTTCGTGAAGTTCGGCGGGTTGAAGTTTCTCGATTCGGTTCACGTGAAGGACGTGCTCGCCGTGCTGCGCTG
GGCGGAAAATCCGCGCGATCGTGTGGCCGGGTTCCGCGTCGTGCAGTTGCTGCCGGGCGTCGGGCCGGCCACGGCGGCGA
AGGTGCTCGACGAAATCGTCGCGCGCGCGGGCAGCGGCAATCCTGCCGACACGGCCGGCGGCGCGCTGGCCGCGTTCGCC
GCGCCGGCTCGCGCGCAGGAAGACTGGCATCCGTTCGTCAAGCTGATGTCGAGCGTGTACGGGCGCCAGACGCCGTGGCC
TGCCGAGTTCGAGATGGTGCGCCGCTGGTATGAGCCGCATCTCGAGCGCAACCACGAGGACGCGGCGATCCGTCATGCGG
ACGTGTTGCAGATGGAGAGCATCGCGGGCACGTATGCGTCGCGTGAACGCTTTCTGACCGAGTTGACGCTCGATCCGCCC
GACGCCACCAGCGACGAATCCGGCGTGCCCCTGATCGACGAGGACTACCTGATCCTCTCCACGATCCATTCCGCGAAGGG
CCAGGAGTGGCGCAACGTGTTCGTGCTGAACGGCGTGGACGGCTGCATTCCGTCCGATCTCGGCACCGGCAGCGAAGAGG
AAATCGACGAGGAGCGCCGTCTGTTGTACGTGGCGATGACGCGCGCGAAAGAAGACCTGCATATCGTCGTGCCGCAGCGC
TTTTACGTGCACAACCAGACGCATATGGGCGATCGTCACGTGTGGGCGTCGCGCACGCGCTTCATTCCCGCGCATCTGAT
GCCGCTGTTCGACGCCTATGCATGGCCGCGCGTGCCGGTTCCGAGCGCGCCCACGGCGGCGGGGCTGGCCGCGGCCGCGC
AGGCGAAGATCGAGATCGCGGCGAAGCTCAGGAAGATGTGGGATTGA

Upstream 100 bases:

>100_bases
GATGAAGCGCGGCCGAAAAAAAGGGTCGCTTGTCTGTGAGGGTTGTATGGATATACAGTATGCGTCGCCGTACTGAATCC
ACCCACCGCCCAAACCGATC

Downstream 100 bases:

>100_bases
GGGCTGGGCGCCGGCGCCGTTCTGCTCTTCACGCGGCGCTGCGCGCCGACTGGTCGGCTACCGGTGACCCGGCGGCCGTT
CCAAACGCCTTACTCTTCAG

Product: putative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MLPVAESPPFDTAAWLARLNDAQREAVEYGADTPTAPPGALLVIAGAGSGKTNTLAHRVANLVVNGADPRRILLLTFSRR
AALEMIRRVTRIAGAALGTRAALAQGLTWSGTFHSVGARLLREYADLIGLAPAFTINDREDSADLMNLVRHELGLSAKER
RFPAKGTCFAIYSRVVNTGASLSQVLDKAFPWCREWEADLRMLFAAYVEAKQKQSVLDYDDLLLYWSLMAAEPAIAADLS
ARFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGATVRNILDFPAHFDPPARQVTLERNYRSTAPIL
AASNAVIELASERYTKNLWTDKASAQRPRLVSVADEADQARYVVEQVLEAREQGMKLKSQAVLFRAAHHSAALEIELTRR
NIPFVKFGGLKFLDSVHVKDVLAVLRWAENPRDRVAGFRVVQLLPGVGPATAAKVLDEIVARAGSGNPADTAGGALAAFA
APARAQEDWHPFVKLMSSVYGRQTPWPAEFEMVRRWYEPHLERNHEDAAIRHADVLQMESIAGTYASRERFLTELTLDPP
DATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVDGCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVVPQR
FYVHNQTHMGDRHVWASRTRFIPAHLMPLFDAYAWPRVPVPSAPTAAGLAAAAQAKIEIAAKLRKMWD

Sequences:

>Translated_708_residues
MLPVAESPPFDTAAWLARLNDAQREAVEYGADTPTAPPGALLVIAGAGSGKTNTLAHRVANLVVNGADPRRILLLTFSRR
AALEMIRRVTRIAGAALGTRAALAQGLTWSGTFHSVGARLLREYADLIGLAPAFTINDREDSADLMNLVRHELGLSAKER
RFPAKGTCFAIYSRVVNTGASLSQVLDKAFPWCREWEADLRMLFAAYVEAKQKQSVLDYDDLLLYWSLMAAEPAIAADLS
ARFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGATVRNILDFPAHFDPPARQVTLERNYRSTAPIL
AASNAVIELASERYTKNLWTDKASAQRPRLVSVADEADQARYVVEQVLEAREQGMKLKSQAVLFRAAHHSAALEIELTRR
NIPFVKFGGLKFLDSVHVKDVLAVLRWAENPRDRVAGFRVVQLLPGVGPATAAKVLDEIVARAGSGNPADTAGGALAAFA
APARAQEDWHPFVKLMSSVYGRQTPWPAEFEMVRRWYEPHLERNHEDAAIRHADVLQMESIAGTYASRERFLTELTLDPP
DATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVDGCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVVPQR
FYVHNQTHMGDRHVWASRTRFIPAHLMPLFDAYAWPRVPVPSAPTAAGLAAAAQAKIEIAAKLRKMWD
>Mature_708_residues
MLPVAESPPFDTAAWLARLNDAQREAVEYGADTPTAPPGALLVIAGAGSGKTNTLAHRVANLVVNGADPRRILLLTFSRR
AALEMIRRVTRIAGAALGTRAALAQGLTWSGTFHSVGARLLREYADLIGLAPAFTINDREDSADLMNLVRHELGLSAKER
RFPAKGTCFAIYSRVVNTGASLSQVLDKAFPWCREWEADLRMLFAAYVEAKQKQSVLDYDDLLLYWSLMAAEPAIAADLS
ARFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGATVRNILDFPAHFDPPARQVTLERNYRSTAPIL
AASNAVIELASERYTKNLWTDKASAQRPRLVSVADEADQARYVVEQVLEAREQGMKLKSQAVLFRAAHHSAALEIELTRR
NIPFVKFGGLKFLDSVHVKDVLAVLRWAENPRDRVAGFRVVQLLPGVGPATAAKVLDEIVARAGSGNPADTAGGALAAFA
APARAQEDWHPFVKLMSSVYGRQTPWPAEFEMVRRWYEPHLERNHEDAAIRHADVLQMESIAGTYASRERFLTELTLDPP
DATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVDGCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVVPQR
FYVHNQTHMGDRHVWASRTRFIPAHLMPLFDAYAWPRVPVPSAPTAAGLAAAAQAKIEIAAKLRKMWD

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=671, Percent_Identity=32.0417287630402, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI48994965, Length=635, Percent_Identity=30.2362204724409, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322369, Length=471, Percent_Identity=27.8131634819533, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78185; Mature: 78185

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPVAESPPFDTAAWLARLNDAQREAVEYGADTPTAPPGALLVIAGAGSGKTNTLAHRVA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
NLVVNGADPRRILLLTFSRRAALEMIRRVTRIAGAALGTRAALAQGLTWSGTFHSVGARL
HHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LREYADLIGLAPAFTINDREDSADLMNLVRHELGLSAKERRFPAKGTCFAIYSRVVNTGA
HHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCH
SLSQVLDKAFPWCREWEADLRMLFAAYVEAKQKQSVLDYDDLLLYWSLMAAEPAIAADLS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
ARFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGATVRNILDFPAHF
HHHCHHHHHCCCCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHCCHHHHHHHHCCCCC
DPPARQVTLERNYRSTAPILAASNAVIELASERYTKNLWTDKASAQRPRLVSVADEADQA
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHH
RYVVEQVLEAREQGMKLKSQAVLFRAAHHSAALEIELTRRNIPFVKFGGLKFLDSVHVKD
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCEEEECCHHHHHHHHHHH
VLAVLRWAENPRDRVAGFRVVQLLPGVGPATAAKVLDEIVARAGSGNPADTAGGALAAFA
HHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
APARAQEDWHPFVKLMSSVYGRQTPWPAEFEMVRRWYEPHLERNHEDAAIRHADVLQMES
CCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IAGTYASRERFLTELTLDPPDATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVD
HHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCC
GCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVVPQRFYVHNQTHMGDRHVWASRTR
CCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCEEEEECCEEEECCCCCCCCCCCHHHHCC
FIPAHLMPLFDAYAWPRVPVPSAPTAAGLAAAAQAKIEIAAKLRKMWD
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLPVAESPPFDTAAWLARLNDAQREAVEYGADTPTAPPGALLVIAGAGSGKTNTLAHRVA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
NLVVNGADPRRILLLTFSRRAALEMIRRVTRIAGAALGTRAALAQGLTWSGTFHSVGARL
HHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LREYADLIGLAPAFTINDREDSADLMNLVRHELGLSAKERRFPAKGTCFAIYSRVVNTGA
HHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCH
SLSQVLDKAFPWCREWEADLRMLFAAYVEAKQKQSVLDYDDLLLYWSLMAAEPAIAADLS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
ARFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGATVRNILDFPAHF
HHHCHHHHHCCCCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHCCHHHHHHHHCCCCC
DPPARQVTLERNYRSTAPILAASNAVIELASERYTKNLWTDKASAQRPRLVSVADEADQA
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHH
RYVVEQVLEAREQGMKLKSQAVLFRAAHHSAALEIELTRRNIPFVKFGGLKFLDSVHVKD
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCEEEECCHHHHHHHHHHH
VLAVLRWAENPRDRVAGFRVVQLLPGVGPATAAKVLDEIVARAGSGNPADTAGGALAAFA
HHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
APARAQEDWHPFVKLMSSVYGRQTPWPAEFEMVRRWYEPHLERNHEDAAIRHADVLQMES
CCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IAGTYASRERFLTELTLDPPDATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVD
HHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCC
GCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVVPQRFYVHNQTHMGDRHVWASRTR
CCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCEEEEECCEEEECCCCCCCCCCCHHHHCC
FIPAHLMPLFDAYAWPRVPVPSAPTAAGLAAAAQAKIEIAAKLRKMWD
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]