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The map label for this gene is otsA [H]

Identifier: 91784557

GI number: 91784557

Start: 3548887

End: 3550347

Strand: Reverse

Name: otsA [H]

Synonym: Bxe_A1242

Alternate gene names: 91784557

Gene position: 3550347-3548887 (Counterclockwise)

Preceding gene: 91784558

Following gene: 91784554

Centisome position: 72.52

GC content: 64.96

Gene sequence:

>1461_bases
ATGGGCCGACTGATCATCGTATCGAACCGGGTTGCGCCGATCTCGGAGGGCGGCCCGGCGGCGGGTGGTCTGGCGGTCGG
CGTGTACGACGCACTGAAGGAAACCGGCGGCATGTGGTTCGGCTGGAGCGGCGACGTGCTCAGTTCGGGCCAGCCGCAGA
TCAAGGTCGAAGAGCGCGGCCCGGTGACCTTCGCGACGATCGCGCTGATGCGCCGCGATTACGATCAGTACTACCGTGGC
TTCTCGAACGCGACGCTGTGGCCCGCGTTCCACTATCGCGCCGACCTGCTGCAATACGACCGGCACGACTTCGAGGGCTA
CTGGCGCGTCAATGCCTGGCTCGCGCAACAACTCGTGCCGCTGTTGCGCGAAGACGATGTGATCTGGGTTCACGACTATC
ACCTGATTCCATTTGCGCAGGCGCTGCGTGCGGCGGGCGTGAAGAATCGCATCGGCTTTTTTCTGCACATTCCGTTTCCG
GCTTCCCAGGTGCTGCTGGCAGTGCCGCCGCATCGCGAACTTGTCGAAGCGCTGTGTTCGTTCGATCTGCTCGGCTTCCA
GACTGCGCCGGATTTGCGCGCGTTCTGCGACTACATCGTCAATGAGGCGAACGGCACCGCCGACCCATCGGCAAGCGGTC
CGCTGACGATCCACGCGTTCGGCCGCACGCTGCGGGCGGCAGCTTATCCGATCGGCGTCTATCCGGACGAGATCGCCGAA
CTCGCGAAAGCGGGCGAGCGCGGCAAACCGGTGCGCACGATGAAGGCGACGCTGCACTCGCGCAAGCTGATCATGAGCGT
GGACCGGCTCGATTACTCGAAGGGGCTGGTCGAGCGTTTTCGCGCCTTCGAGCGTCTGCTCGAACACTCGACCGCGCAAC
GCAACAAGGTGTCGTTCCTGCAGATCGCGCCGCCGACGCGCGCCGACATGCACGCATACCAGGACATCCGCTTGCAGCTC
GAAGGGGAGTCGGGGCGCATCAACGGGCGCTTCGCCGAACTGGACTGGACGCCGATTCTCTACATCCACAAGCAATACGA
GCGCTCGGTGCTGGCCGCGCTGTTCCGCACCGCGCATGTCGGCTATGTCACGCCGTTGCGTGACGGCATGAACCTCGTCG
CCAAGGAGTACGTATCGGCGCAGGATCCGGAGAATCCCGGCGTGCTCGTGCTGTCACGCTTTGCCGGCGCCGCGCAGGAA
CTGGACGGCGCGCTGATCGTCAATCCGGTCGACATCGACGGCATGGCCGAGGCGCTCGCACGGGCGCTCGACATGCCGCT
CGCGGAGCGTCAGGCGCGTCATCGCGACATGATGGTGCAGTTGCGCGAGAACAATGTGTCGGTGTGGCGCGATAACTTCA
TGCGCGATCTGCAGGGCGTCGCTGGTGCAAAGGGCACCCGGAATGGGAAGGCGCGCGCAGTGGCGGGGAAAAAGGCGGCG
CGCGAAACGCAGGCCGGGTAG

Upstream 100 bases:

>100_bases
CTCGACTGGCTGGCATCGATCGTCGCGGCCGCGCGCGCGGCATGATGGCCATGCCGCATTGCTCAGAACCTATAACAAAC
CGCATCGGGAATCGTGCTTC

Downstream 100 bases:

>100_bases
GTCCATACGGGCCGAGGGGCGTGGCGTGTAAAAACAGAAAAGCCGCTTTTGAAGCGGCTTTTCCTTTGGTGCCGGGACGA
CGTGCCGGGACAGCGGCTCG

Product: Alpha,alpha-trehalose-phosphate synthase(UDP- forming)

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGDVLSSGQPQIKVEERGPVTFATIALMRRDYDQYYRG
FSNATLWPAFHYRADLLQYDRHDFEGYWRVNAWLAQQLVPLLREDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFP
ASQVLLAVPPHRELVEALCSFDLLGFQTAPDLRAFCDYIVNEANGTADPSASGPLTIHAFGRTLRAAAYPIGVYPDEIAE
LAKAGERGKPVRTMKATLHSRKLIMSVDRLDYSKGLVERFRAFERLLEHSTAQRNKVSFLQIAPPTRADMHAYQDIRLQL
EGESGRINGRFAELDWTPILYIHKQYERSVLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPENPGVLVLSRFAGAAQE
LDGALIVNPVDIDGMAEALARALDMPLAERQARHRDMMVQLRENNVSVWRDNFMRDLQGVAGAKGTRNGKARAVAGKKAA
RETQAG

Sequences:

>Translated_486_residues
MGRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGDVLSSGQPQIKVEERGPVTFATIALMRRDYDQYYRG
FSNATLWPAFHYRADLLQYDRHDFEGYWRVNAWLAQQLVPLLREDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFP
ASQVLLAVPPHRELVEALCSFDLLGFQTAPDLRAFCDYIVNEANGTADPSASGPLTIHAFGRTLRAAAYPIGVYPDEIAE
LAKAGERGKPVRTMKATLHSRKLIMSVDRLDYSKGLVERFRAFERLLEHSTAQRNKVSFLQIAPPTRADMHAYQDIRLQL
EGESGRINGRFAELDWTPILYIHKQYERSVLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPENPGVLVLSRFAGAAQE
LDGALIVNPVDIDGMAEALARALDMPLAERQARHRDMMVQLRENNVSVWRDNFMRDLQGVAGAKGTRNGKARAVAGKKAA
RETQAG
>Mature_485_residues
GRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGDVLSSGQPQIKVEERGPVTFATIALMRRDYDQYYRGF
SNATLWPAFHYRADLLQYDRHDFEGYWRVNAWLAQQLVPLLREDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPA
SQVLLAVPPHRELVEALCSFDLLGFQTAPDLRAFCDYIVNEANGTADPSASGPLTIHAFGRTLRAAAYPIGVYPDEIAEL
AKAGERGKPVRTMKATLHSRKLIMSVDRLDYSKGLVERFRAFERLLEHSTAQRNKVSFLQIAPPTRADMHAYQDIRLQLE
GESGRINGRFAELDWTPILYIHKQYERSVLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPENPGVLVLSRFAGAAQEL
DGALIVNPVDIDGMAEALARALDMPLAERQARHRDMMVQLRENNVSVWRDNFMRDLQGVAGAKGTRNGKARAVAGKKAAR
ETQAG

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=460, Percent_Identity=53.695652173913, Blast_Score=483, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI115533560, Length=361, Percent_Identity=31.0249307479224, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI115533558, Length=361, Percent_Identity=31.0249307479224, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71987012, Length=439, Percent_Identity=28.246013667426, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6319602, Length=472, Percent_Identity=32.2033898305085, Blast_Score=272, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6320279, Length=436, Percent_Identity=26.3761467889908, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323537, Length=436, Percent_Identity=25.6880733944954, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323917, Length=405, Percent_Identity=25.9259259259259, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19920676, Length=485, Percent_Identity=34.8453608247423, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 54080; Mature: 53949

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGDVLSSGQPQIKVEERG
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCC
PVTFATIALMRRDYDQYYRGFSNATLWPAFHYRADLLQYDRHDFEGYWRVNAWLAQQLVP
CCHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
LLREDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPASQVLLAVPPHRELVEALCS
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHC
FDLLGFQTAPDLRAFCDYIVNEANGTADPSASGPLTIHAFGRTLRAAAYPIGVYPDEIAE
CHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHH
LAKAGERGKPVRTMKATLHSRKLIMSVDRLDYSKGLVERFRAFERLLEHSTAQRNKVSFL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
QIAPPTRADMHAYQDIRLQLEGESGRINGRFAELDWTPILYIHKQYERSVLAALFRTAHV
EECCCCCHHHHHCEEEEEEEECCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHCC
GYVTPLRDGMNLVAKEYVSAQDPENPGVLVLSRFAGAAQELDGALIVNPVDIDGMAEALA
CEECCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCHHHHCCCEEEEECCCCCHHHHHHH
RALDMPLAERQARHRDMMVQLRENNVSVWRDNFMRDLQGVAGAKGTRNGKARAVAGKKAA
HHHCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHH
RETQAG
HHCCCC
>Mature Secondary Structure 
GRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGDVLSSGQPQIKVEERG
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEECCC
PVTFATIALMRRDYDQYYRGFSNATLWPAFHYRADLLQYDRHDFEGYWRVNAWLAQQLVP
CCHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
LLREDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPASQVLLAVPPHRELVEALCS
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHC
FDLLGFQTAPDLRAFCDYIVNEANGTADPSASGPLTIHAFGRTLRAAAYPIGVYPDEIAE
CHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHH
LAKAGERGKPVRTMKATLHSRKLIMSVDRLDYSKGLVERFRAFERLLEHSTAQRNKVSFL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
QIAPPTRADMHAYQDIRLQLEGESGRINGRFAELDWTPILYIHKQYERSVLAALFRTAHV
EECCCCCHHHHHCEEEEEEEECCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHCC
GYVTPLRDGMNLVAKEYVSAQDPENPGVLVLSRFAGAAQELDGALIVNPVDIDGMAEALA
CEECCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCHHHHCCCEEEEECCCCCHHHHHHH
RALDMPLAERQARHRDMMVQLRENNVSVWRDNFMRDLQGVAGAKGTRNGKARAVAGKKAA
HHHCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHH
RETQAG
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA