Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 91784528

GI number: 91784528

Start: 3516398

End: 3517099

Strand: Reverse

Name: phoB [H]

Synonym: Bxe_A1271

Alternate gene names: 91784528

Gene position: 3517099-3516398 (Counterclockwise)

Preceding gene: 91784529

Following gene: 91784527

Centisome position: 71.84

GC content: 62.11

Gene sequence:

>702_bases
ATGCCCAGCAGCATTCTCGTCATTGAAGATGAGCCCGCCATTTCCGAATTGATTTCGGTCAATCTTCAACACGCCGGACA
CTGCCCGATTCGCGCGTACAACGCGGAGCAGGCGCAGAACCTGATCAGCGACGTATTGCCCGACCTCGTCCTGCTCGACT
GGATGTTGCCGGGAAAATCGGGCATTGCGTTCGCGCGTGATCTGCGCAACAACGAGCGCACGAAGCATATCCCCATCATC
ATGCTGACCGCACGCGGCGACGAGCAGGACAAGGTGCTCGGCCTCGAAATCGGCGCCGACGACTACGTCACCAAACCGTT
CTCGCCGAAAGAACTGATGGCGCGCATCAAGGCGGTGTTGCGCCGCCGTGCGCCGCAGTTGACCGAAGACGTGGTCGCGA
TCAACGGCCTCAAGCTCGATCCGGCCACACACCGCGTGGCCGCGCATGCCGAAGGCAGCGAGATCAAGCTCGATCTCGGT
CCGACCGAATTCCGTCTGCTGCACTTCTTCATGACGCATCCGGAGCGCGTGCACAGCCGCACGCAGTTGCTCGATCAGGT
GTGGGGCGATCACGTGTTCGTCGAAGAGCGTACGGTCGACGTGCATATCAAGCGCCTGCGCGCGGCGCTCAAGCCGGCCG
GCTGCGATGCTATGATTGAGACGGTGCGGGGCAGCGGTTACCGGCTCGCGAAGAGCGCCTGA

Upstream 100 bases:

>100_bases
TCATTTACATCGTCAAGGGCACGGACGTGCGGCACAAGTCACGCGACGCGCTCGAACGCGAAGCGCTCAGTTAATCCAGA
TATAAGGACCAGAGGTGCCG

Downstream 100 bases:

>100_bases
CCGGCGTTCACGGTGGCGGCGTGCGGGCCGCACGCGCCGGGTACGTCATCTTCGCAACGCTTCTCGTCACCCTTCGCAAT
GTCAGGCGCCGCGATTCAGA

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLKLDPATHRVAAHAEGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA

Sequences:

>Translated_233_residues
MPSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLKLDPATHRVAAHAEGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA
>Mature_232_residues
PSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPIIM
LTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLKLDPATHRVAAHAEGSEIKLDLGP
TEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=57.8260869565217, Blast_Score=283, Evalue=8e-78,
Organism=Escherichia coli, GI1788394, Length=231, Percent_Identity=41.5584415584416, Blast_Score=163, Evalue=9e-42,
Organism=Escherichia coli, GI1789809, Length=231, Percent_Identity=38.961038961039, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=38.1578947368421, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=36, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=33.1838565022422, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=34.5291479820628, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI2367329, Length=240, Percent_Identity=37.9166666666667, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=34.7639484978541, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789402, Length=228, Percent_Identity=33.7719298245614, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1790552, Length=228, Percent_Identity=32.4561403508772, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI145693140, Length=244, Percent_Identity=31.1475409836066, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=34.5132743362832, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25997; Mature: 25866

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCCEEEEECCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVAINGLKLDPATHRVAAHAEGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCCHHHHHHHCCCEEECCHHHHEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure 
PSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCEEEEECCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVAINGLKLDPATHRVAAHAEGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCCHHHHHHHCCCEEECCHHHHEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]