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The map label for this gene is thrS [H]

Identifier: 91784428

GI number: 91784428

Start: 3400817

End: 3402724

Strand: Reverse

Name: thrS [H]

Synonym: Bxe_A1371

Alternate gene names: 91784428

Gene position: 3402724-3400817 (Counterclockwise)

Preceding gene: 91784429

Following gene: 91784427

Centisome position: 69.5

GC content: 60.22

Gene sequence:

>1908_bases
ATGGTTTCGATACGTCTGCCTGACGGTTCTGTTCGACAGTACGAGCATCCGGTGACCGTCGCCGAAGTGGCCGCCTCGAT
TGGCCCCGGCCTCGCGAAGGCCGCGCTCGGCGGCAAGATCGACGGTGAGTTGGTCGATACGTCCGCGCTGATCGATCACG
ACGTGGCGCTTGCCATCGTCACGGAGAAAGACGCAGACGGTCTCGATATCATCCGGCACTCCACGGCGCACTTGCTCGCG
TACGCGGTGAAGGATCTGTACCCGGAAGCGCAGGTCACGATCGGCCCGGTCATCGACAACGGCTTTTACTACGACTTCGC
ATATAACCGTCCGTTCACGCCGGAAGATCTCGAGAAGATCGAAAAGCGCATGCAGGAACTCGCGAAGAAAGACGAGCCGG
TTTCGCGCCGCGTGGTGTCGCGCGACGAAGCGGTGGACTACTTCAAGAGCATCGGCGAAAAGTACAAGGCCGAGATCATC
GAATCGATTCCCGCCACCGACGAGATCAAGCTGTACTCGCATGGCGGCTTCACGGATCTGTGCCGCGGCCCGCACGTGCC
GTCTACCGGCAAGCTGAAAGTCTTCAAGCTGATGAAGGTCGCGGGTGCCTACTGGCGCGGCGATTCGAAGAACGAACAAT
TGCAGCGCATCTACGGTACGGCCTGGACGAAGAAGGAAGACCAGGAAGCGTACCTGCACATGCTCGAAGAGGCGGAAAAG
CGCGACCACCGCAAGCTCGGCAAGCAACTCGATCTGTTCCACATGCAGGACGAGTCGCCGGGCATGGTGTTCTGGCATCC
GCGTGGCTGGACGTTGTGGCAGCAGGTCGAGCAGTACATGCGCCGTCGCGTGAACGACGCGGGGTACCTCGAAATCAAGA
CGCCGATGATCATGGACCGCTCGCTCTGGGAAGCGTCGGGCCACTGGCAGAACTATCGTGAAAACATGTTCACGACGGAA
TCGGAAAAGCGCGACTACGCGATCAAGCCGATGAACTGCCCGGGTCACGTGCAGGTGTTCAACCACGGTCTGCGCTCGTA
TCGCGATCTGCCGCTGCGTTACGCCGAATTCGGCTCGTGCCATCGCAACGAATCGTCGGGCGCGCTGCATGGACTGATGC
GCGTGCGCGGGTTCGTGCAGGACGACGCGCACATTTTCTGTACCGAAGACCAGTTCATCAGCGAATCGATCGCGTTCAAC
ACGCTGGCGATGAGCGTCTACAAGGATTTCGGCTTCGACCATGTCGAGATCAAGCTGTCGCTGCGCCCGGACGCGCGCGC
CGGCACGGACGAGACGTGGGATCGCGCGGAGCAGGGGTTGCGCGAGGCGCTGACGGCCTGCGGTGTGACGTGGGAAGAAC
TGCCGGGCGAGGGCGCGTTCTACGGCCCGAAGGTCGAATACCACATCAAGGATGCGCTTGGCCGCTCGTGGCAATGCGGC
ACGTTGCAGCTCGATATGGTGTTGCCGGAGCGCCTCGGCGCGGAATACGTCGCCGAAGACAATAGCCGCCGCCGGCCGAT
CATGCTGCACCGGGCAATCGTCGGATCAATGGAGCGTTTCCTTGGCATTCTGATCGAGCACCACGCTGGTGCAATGCCTG
CGTGGCTCGCGCCAATGCAGGTCGTTGTGATGAATATCGCGGAAAGTCAGACCGAATATGCGCAGTCTTTAGCCCAATCG
TTGCAAAAACAAGGGGTTAGAGTAGCGGCTGATTTGCGCAACGAGAAGATTAGCTATAAAATACGGGAGCACACGCTGGA
GAAGGTGCCGTACCTGCTTGTGGTCGGCGACAAAGAGCGTGAAGCCCAAACGGTAGCCGTGCGTGCCCGTGGTGGTGTCG
ATCTGGGTGTGATGCCCCTCGACACTTTCATTGAGCGTCTGCGCCAGGATGTGCAGTCGTTCAACTGA

Upstream 100 bases:

>100_bases
CAGGAAATGTGAATGCGGCCCCTCGAAAGCGGGGCCGCATTTTTTTTAGCTTGTTTGATGGATGTATGTGCCGCCGCCGG
TCGGCACCACGGAGAACGCA

Downstream 100 bases:

>100_bases
GCCACTTGGCAGCCCGGCTCGTTTTTTTAATTTTTAGAGGAAACGTAACATCGCTACTGATAAGTCTGCGCACCGCATCA
ACGGTGAAATTACGGCACCC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKIDGELVDTSALIDHDVALAIVTEKDADGLDIIRHSTAHLLA
YAVKDLYPEAQVTIGPVIDNGFYYDFAYNRPFTPEDLEKIEKRMQELAKKDEPVSRRVVSRDEAVDYFKSIGEKYKAEII
ESIPATDEIKLYSHGGFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQEAYLHMLEEAEK
RDHRKLGKQLDLFHMQDESPGMVFWHPRGWTLWQQVEQYMRRRVNDAGYLEIKTPMIMDRSLWEASGHWQNYRENMFTTE
SEKRDYAIKPMNCPGHVQVFNHGLRSYRDLPLRYAEFGSCHRNESSGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFN
TLAMSVYKDFGFDHVEIKLSLRPDARAGTDETWDRAEQGLREALTACGVTWEELPGEGAFYGPKVEYHIKDALGRSWQCG
TLQLDMVLPERLGAEYVAEDNSRRRPIMLHRAIVGSMERFLGILIEHHAGAMPAWLAPMQVVVMNIAESQTEYAQSLAQS
LQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKEREAQTVAVRARGGVDLGVMPLDTFIERLRQDVQSFN

Sequences:

>Translated_635_residues
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKIDGELVDTSALIDHDVALAIVTEKDADGLDIIRHSTAHLLA
YAVKDLYPEAQVTIGPVIDNGFYYDFAYNRPFTPEDLEKIEKRMQELAKKDEPVSRRVVSRDEAVDYFKSIGEKYKAEII
ESIPATDEIKLYSHGGFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQEAYLHMLEEAEK
RDHRKLGKQLDLFHMQDESPGMVFWHPRGWTLWQQVEQYMRRRVNDAGYLEIKTPMIMDRSLWEASGHWQNYRENMFTTE
SEKRDYAIKPMNCPGHVQVFNHGLRSYRDLPLRYAEFGSCHRNESSGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFN
TLAMSVYKDFGFDHVEIKLSLRPDARAGTDETWDRAEQGLREALTACGVTWEELPGEGAFYGPKVEYHIKDALGRSWQCG
TLQLDMVLPERLGAEYVAEDNSRRRPIMLHRAIVGSMERFLGILIEHHAGAMPAWLAPMQVVVMNIAESQTEYAQSLAQS
LQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKEREAQTVAVRARGGVDLGVMPLDTFIERLRQDVQSFN
>Mature_635_residues
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKIDGELVDTSALIDHDVALAIVTEKDADGLDIIRHSTAHLLA
YAVKDLYPEAQVTIGPVIDNGFYYDFAYNRPFTPEDLEKIEKRMQELAKKDEPVSRRVVSRDEAVDYFKSIGEKYKAEII
ESIPATDEIKLYSHGGFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQEAYLHMLEEAEK
RDHRKLGKQLDLFHMQDESPGMVFWHPRGWTLWQQVEQYMRRRVNDAGYLEIKTPMIMDRSLWEASGHWQNYRENMFTTE
SEKRDYAIKPMNCPGHVQVFNHGLRSYRDLPLRYAEFGSCHRNESSGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFN
TLAMSVYKDFGFDHVEIKLSLRPDARAGTDETWDRAEQGLREALTACGVTWEELPGEGAFYGPKVEYHIKDALGRSWQCG
TLQLDMVLPERLGAEYVAEDNSRRRPIMLHRAIVGSMERFLGILIEHHAGAMPAWLAPMQVVVMNIAESQTEYAQSLAQS
LQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKEREAQTVAVRARGGVDLGVMPLDTFIERLRQDVQSFN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=633, Percent_Identity=39.4944707740916, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI38202255, Length=632, Percent_Identity=38.6075949367089, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI20070344, Length=649, Percent_Identity=37.1340523882897, Blast_Score=409, Evalue=1e-114,
Organism=Escherichia coli, GI1788013, Length=634, Percent_Identity=58.9905362776025, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=155, Percent_Identity=27.0967741935484, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71984184, Length=633, Percent_Identity=39.0205371248025, Blast_Score=479, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71984192, Length=633, Percent_Identity=39.0205371248025, Blast_Score=479, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322112, Length=629, Percent_Identity=38.4737678855326, Blast_Score=453, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=40.0503778337531, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24583842, Length=631, Percent_Identity=39.4611727416799, Blast_Score=480, Evalue=1e-135,
Organism=Drosophila melanogaster, GI28574141, Length=631, Percent_Identity=39.4611727416799, Blast_Score=480, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24583839, Length=631, Percent_Identity=39.4611727416799, Blast_Score=480, Evalue=1e-135,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72213; Mature: 72213

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKIDGELVDTSALIDHDVALAIV
CEEEECCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCEECHHHHHCCCEEEEEE
TEKDADGLDIIRHSTAHLLAYAVKDLYPEAQVTIGPVIDNGFYYDFAYNRPFTPEDLEKI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHH
EKRMQELAKKDEPVSRRVVSRDEAVDYFKSIGEKYKAEIIESIPATDEIKLYSHGGFTDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQEAYLHMLEEAEK
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RDHRKLGKQLDLFHMQDESPGMVFWHPRGWTLWQQVEQYMRRRVNDAGYLEIKTPMIMDR
HHHHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHCH
SLWEASGHWQNYRENMFTTESEKRDYAIKPMNCPGHVQVFNHGLRSYRDLPLRYAEFGSC
HHHHCCCCHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
HRNESSGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFNTLAMSVYKDFGFDHVEIKLS
CCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
LRPDARAGTDETWDRAEQGLREALTACGVTWEELPGEGAFYGPKVEYHIKDALGRSWQCG
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEHHHCCCCCCCC
TLQLDMVLPERLGAEYVAEDNSRRRPIMLHRAIVGSMERFLGILIEHHAGAMPAWLAPMQ
CEEEEEECHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VVVMNIAESQTEYAQSLAQSLQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKER
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCEEEHHHHHHHHHCCEEEEECCCCC
EAQTVAVRARGGVDLGVMPLDTFIERLRQDVQSFN
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKIDGELVDTSALIDHDVALAIV
CEEEECCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCEECHHHHHCCCEEEEEE
TEKDADGLDIIRHSTAHLLAYAVKDLYPEAQVTIGPVIDNGFYYDFAYNRPFTPEDLEKI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHH
EKRMQELAKKDEPVSRRVVSRDEAVDYFKSIGEKYKAEIIESIPATDEIKLYSHGGFTDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQEAYLHMLEEAEK
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RDHRKLGKQLDLFHMQDESPGMVFWHPRGWTLWQQVEQYMRRRVNDAGYLEIKTPMIMDR
HHHHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHCH
SLWEASGHWQNYRENMFTTESEKRDYAIKPMNCPGHVQVFNHGLRSYRDLPLRYAEFGSC
HHHHCCCCHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCC
HRNESSGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFNTLAMSVYKDFGFDHVEIKLS
CCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
LRPDARAGTDETWDRAEQGLREALTACGVTWEELPGEGAFYGPKVEYHIKDALGRSWQCG
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEHHHCCCCCCCC
TLQLDMVLPERLGAEYVAEDNSRRRPIMLHRAIVGSMERFLGILIEHHAGAMPAWLAPMQ
CEEEEEECHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VVVMNIAESQTEYAQSLAQSLQKQGVRVAADLRNEKISYKIREHTLEKVPYLLVVGDKER
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCEEEHHHHHHHHHCCEEEEECCCCC
EAQTVAVRARGGVDLGVMPLDTFIERLRQDVQSFN
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA