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The map label for this gene is nitA [H]

Identifier: 91784392

GI number: 91784392

Start: 3362704

End: 3363750

Strand: Reverse

Name: nitA [H]

Synonym: Bxe_A1408

Alternate gene names: 91784392

Gene position: 3363750-3362704 (Counterclockwise)

Preceding gene: 91784393

Following gene: 91784391

Centisome position: 68.71

GC content: 64.18

Gene sequence:

>1047_bases
ATGTCCGACAAATCGACCGGCAAGCGCATCGTGCGCGCCGCGGCGGTCCAGATCGCACCGGACTTCGAGCGCAGCGGCGG
CACGCTCGAAAAGGTTTGCGAGGCCATCGAACAGGCGGCGCGCGAAGGCGTGCAACTGATCGTATTTCCCGAAACCTTCG
TGCCGTATTACCCGTACTTCTCTTTCGTGCGGCCGCCCGTGGCCTCCGGCGCCGATCACATGAAACTCTACGAAGAGGCC
GTGGTGGTGCCGGGTCCCGTGACGCAGGCGGTGGCCGAGCAGGCGCGTTTGCACCGGATGGTGGTGGTGCTCGGCGTCAA
CGAGCGCGATCACGGCAGCCTCTACAACACGCAACTGATCTTCGACGTGGACGGCCAGATCGTGCTCAAGCGCCGCAAGA
TCACCCCGACGTTTCATGAACGGATGATCTGGGGCCAAGGCGACGCGGCCGGGCTGACGGTGGCGCGCACCGCGGTGGCA
CGCGTGGGCGCGCTCGCCTGCTGGGAGCATTACAACCCGCTCGCGCGTTATGCGTTGATGACGCAGCACGAAGAGATTCA
TTGCAGCCAGTTTCCCGGCTCGCTGGTGGGGCCGATCTTCGCCGATCAGATCGAGGTGACGATCCGCCATCACGCGCTGG
AATCGGGATGTTTCGTCGTCAATGCAACGGGATGGCTGAGCGAAGCGCAGATTGCGTCCGTGACTCCGGATACGAATCTG
CAGAGGGCGCTGCGCGGCGGCTGCAATACCGCGATCGTCTCTCCGGAAGGCCAGCATCTTGCCGAACCGTTGCGCGAGGG
CGAGGGCATGGTGATCGCCGATCTCGACATGGCCTTGATCACCAAGCGCAAACGCATGATGGATTCGGTCGGCCATTACG
CGCGCCCCGAACTGCTGAGCCTCGCCATCAACCGGCGGCCCGCGGAAACCGTGGCGCCGATGCAGGCGTGGCCGGCTGTC
CCGGGCGCCGATTTCAATTCAACCGAAGGAGGATGCGATGAACGCCAGCGAACCGCTGTCGGCAGAGAGCCGGCAATTGA
TGACTGA

Upstream 100 bases:

>100_bases
ATCAGCCCGCTCGACGTGCTCGACATCCAGTTGCTCCACCGCAAGGACAACGCGCTGGTCATCCACACCTGGACGGTTTG
ACGAAAGGCGAGTGACCACC

Downstream 100 bases:

>100_bases
ATTGCAGTCCGCCGGTTTGCGGCTCGTCTCGCCGGATTCGGGCGCGGCGAGCCGGCGCGGCGGCGCGGGTCCGTCGGACC
ACAAGGCGGTGACGGTGGAT

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSDKSTGKRIVRAAAVQIAPDFERSGGTLEKVCEAIEQAAREGVQLIVFPETFVPYYPYFSFVRPPVASGADHMKLYEEA
VVVPGPVTQAVAEQARLHRMVVVLGVNERDHGSLYNTQLIFDVDGQIVLKRRKITPTFHERMIWGQGDAAGLTVARTAVA
RVGALACWEHYNPLARYALMTQHEEIHCSQFPGSLVGPIFADQIEVTIRHHALESGCFVVNATGWLSEAQIASVTPDTNL
QRALRGGCNTAIVSPEGQHLAEPLREGEGMVIADLDMALITKRKRMMDSVGHYARPELLSLAINRRPAETVAPMQAWPAV
PGADFNSTEGGCDERQRTAVGREPAIDD

Sequences:

>Translated_348_residues
MSDKSTGKRIVRAAAVQIAPDFERSGGTLEKVCEAIEQAAREGVQLIVFPETFVPYYPYFSFVRPPVASGADHMKLYEEA
VVVPGPVTQAVAEQARLHRMVVVLGVNERDHGSLYNTQLIFDVDGQIVLKRRKITPTFHERMIWGQGDAAGLTVARTAVA
RVGALACWEHYNPLARYALMTQHEEIHCSQFPGSLVGPIFADQIEVTIRHHALESGCFVVNATGWLSEAQIASVTPDTNL
QRALRGGCNTAIVSPEGQHLAEPLREGEGMVIADLDMALITKRKRMMDSVGHYARPELLSLAINRRPAETVAPMQAWPAV
PGADFNSTEGGCDERQRTAVGREPAIDD
>Mature_347_residues
SDKSTGKRIVRAAAVQIAPDFERSGGTLEKVCEAIEQAAREGVQLIVFPETFVPYYPYFSFVRPPVASGADHMKLYEEAV
VVPGPVTQAVAEQARLHRMVVVLGVNERDHGSLYNTQLIFDVDGQIVLKRRKITPTFHERMIWGQGDAAGLTVARTAVAR
VGALACWEHYNPLARYALMTQHEEIHCSQFPGSLVGPIFADQIEVTIRHHALESGCFVVNATGWLSEAQIASVTPDTNLQ
RALRGGCNTAIVSPEGQHLAEPLREGEGMVIADLDMALITKRKRMMDSVGHYARPELLSLAINRRPAETVAPMQAWPAVP
GADFNSTEGGCDERQRTAVGREPAIDD

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=313, Percent_Identity=31.9488817891374, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322027, Length=185, Percent_Identity=31.3513513513514, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 38120; Mature: 37989

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50007 PIPLC_X_DOMAIN ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKSTGKRIVRAAAVQIAPDFERSGGTLEKVCEAIEQAAREGVQLIVFPETFVPYYPYF
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCCCCHH
SFVRPPVASGADHMKLYEEAVVVPGPVTQAVAEQARLHRMVVVLGVNERDHGSLYNTQLI
HHCCCCCCCCCHHHHHHHHCEECCCCHHHHHHHHHHHEEEEEEEECCCCCCCCEECEEEE
FDVDGQIVLKRRKITPTFHERMIWGQGDAAGLTVARTAVARVGALACWEHYNPLARYALM
EECCCEEEEEECCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TQHEEIHCSQFPGSLVGPIFADQIEVTIRHHALESGCFVVNATGWLSEAQIASVTPDTNL
HCCCHHHHHHCCCHHHCCHHHHHHEEEHHHHHHCCCCEEEECCCCCCCCEEEECCCCCHH
QRALRGGCNTAIVSPEGQHLAEPLREGEGMVIADLDMALITKRKRMMDSVGHYARPELLS
HHHHHCCCCEEEECCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHHH
LAINRRPAETVAPMQAWPAVPGADFNSTEGGCDERQRTAVGREPAIDD
HHHCCCCCHHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDKSTGKRIVRAAAVQIAPDFERSGGTLEKVCEAIEQAAREGVQLIVFPETFVPYYPYF
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCCCCHH
SFVRPPVASGADHMKLYEEAVVVPGPVTQAVAEQARLHRMVVVLGVNERDHGSLYNTQLI
HHCCCCCCCCCHHHHHHHHCEECCCCHHHHHHHHHHHEEEEEEEECCCCCCCCEECEEEE
FDVDGQIVLKRRKITPTFHERMIWGQGDAAGLTVARTAVARVGALACWEHYNPLARYALM
EECCCEEEEEECCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TQHEEIHCSQFPGSLVGPIFADQIEVTIRHHALESGCFVVNATGWLSEAQIASVTPDTNL
HCCCHHHHHHCCCHHHCCHHHHHHEEEHHHHHHCCCCEEEECCCCCCCCEEEECCCCCHH
QRALRGGCNTAIVSPEGQHLAEPLREGEGMVIADLDMALITKRKRMMDSVGHYARPELLS
HHHHHCCCCEEEECCCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHHH
LAINRRPAETVAPMQAWPAVPGADFNSTEGGCDERQRTAVGREPAIDD
HHHCCCCCHHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]