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The map label for this gene is pucI [H]

Identifier: 91784370

GI number: 91784370

Start: 3336476

End: 3337987

Strand: Reverse

Name: pucI [H]

Synonym: Bxe_A1430

Alternate gene names: 91784370

Gene position: 3337987-3336476 (Counterclockwise)

Preceding gene: 91784371

Following gene: 91784369

Centisome position: 68.18

GC content: 61.64

Gene sequence:

>1512_bases
ATGAAGCAGACAGCGCAACCCGCCGAGCCGCAGTTCGCAACCGGCGTGCAGGGCAGCAGTCTTTACAACGACGACCTGGC
GCCGACCGGTATCGCGCAGCGCACATGGAAGTGGTATCACTTCGCGGCGCTCTGGGTAGGGATGGTGATGAACATCGCGT
CCTACATGCTCGCCGCTGGTTTGACGGAAGAGGGCATGTCGCCGTGGCAAGCGGTGATGACCGTGCTGCTCGGCAACCTG
ATCGTGCTGGTGCCGATGCTGCTGATCGGCCATGCGGGCGCGAAGCACGGCATTCCGTACGCGGTGCTGGTGCGCTCCTC
GTTCGGCACGCAGGGCGCCAAACTGCCGGCGATGCTGCGCGCGATCGTCGCCTGTGGCTGGTACGGGATTCAGACGTGGC
TCGGCGGCAGCGCGATCTATACGCTGCTGAACATTCTCACCGGCAACGCGCTGCACGGCGCGGCTTTGCCGTTCCTCGAC
ATCTCGCTTGCGCAACTGGCCTGTTTCCTCGTGTTCTGGGCGCTGCAGATCTACTTCATCGTGCATGGCACCGATTCGAT
CCGCTGGCTCGAAAGCTGGTCCGCGCCGATCAAGATCGTGATGTGCATTGCGCTCGTATGGTGGGCGACATCCAAAGCGG
GCGGTCTGGGCTCGATGCTGTCGGCGCCGTCGCAATTCGTGCCGGGCGGCAAGAAAGAGGGCATGTTCTGGATTACCTTC
TGGCCCGGCCTTACCGCAATGGTCGGATTCTGGGCCACCCTCGCATTGAATATTCCCGACTTCACGCGTTTTGCCAGAAC
CCAGCGTGATCAGATCATCGGCCAGTCAGTCGGCTTGCCGGTGCCGATGGCGTTGCTCTCGGTGATCTCGGTGGTGGTCA
CGTCGGCCACCGTGGTGATTTACGGCAAGGCGATCTGGGATCCGATCGACCTGACGAGCCGCATGACCGGCATCGGCGTG
GGCGTCGCGCTGATCATCCTCACACTCGACACCATGTGCTGCAATCTCGCGGCAAATCTCGTCGGCCCGGCCTATGATTT
TTCGAGCCTGTGGCCCAAAGGTATTTCGTATCGCGTGGGCGGCATGATCACCGCGACCATCGCAATCGTGATGATGCCGT
GGAAGATTCTCGCCACCACGCAAGGCTATATCTTTACGTGGCTGGTCGGCTATTCGGCCTTGCTCGGGCCGGTGGCCGGT
ATTCTGATGGTCGACTATTTCCTGATTCGCGGCACGCGCCTCGATACGCGTGAACTGTTCGACGAACACGGCGAGTACAG
CTATACCGGCGGCTGGAATATCGGCGCGGTGGTCGCGCTCGTGATCGGCGTGCTGCCGAATCTGCCGGGCTTTTTGCACA
CGGCGTTTCCGGCGTCGTTCCCGAACGTGCCGGCGATCTTCAATACGCTCTATACGTATGCGTGGTTTGTCGGATTGGCG
TTGGCGTCGATCGTATACAGCGCGTGGATGAAGCTGAGCAAGGGACCCAGCGCGCGCGTGGCGAGTGCATGA

Upstream 100 bases:

>100_bases
TTGGCAGCAAACGCAGCGCTAGTACTGAACCTGTCATTCCCCAAGGCCCGACGCGCCGTGAGAGCCGCGCGGGCCGCAAC
GATCGAGTGGAGATCCCTAG

Downstream 100 bases:

>100_bases
GCAGGAACGAAGTACGTAGTACCGAAACCAGCAGTTCATAAGGAGGCGGCAACATGACGACCCTGATTCGCGGCGGCACG
ATTATCGACGCGGAGAACAC

Product: NCS1 family nucleobase/cation symporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MKQTAQPAEPQFATGVQGSSLYNDDLAPTGIAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEEGMSPWQAVMTVLLGNL
IVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLD
ISLAQLACFLVFWALQIYFIVHGTDSIRWLESWSAPIKIVMCIALVWWATSKAGGLGSMLSAPSQFVPGGKKEGMFWITF
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIIGQSVGLPVPMALLSVISVVVTSATVVIYGKAIWDPIDLTSRMTGIGV
GVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRVGGMITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAG
ILMVDYFLIRGTRLDTRELFDEHGEYSYTGGWNIGAVVALVIGVLPNLPGFLHTAFPASFPNVPAIFNTLYTYAWFVGLA
LASIVYSAWMKLSKGPSARVASA

Sequences:

>Translated_503_residues
MKQTAQPAEPQFATGVQGSSLYNDDLAPTGIAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEEGMSPWQAVMTVLLGNL
IVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLD
ISLAQLACFLVFWALQIYFIVHGTDSIRWLESWSAPIKIVMCIALVWWATSKAGGLGSMLSAPSQFVPGGKKEGMFWITF
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIIGQSVGLPVPMALLSVISVVVTSATVVIYGKAIWDPIDLTSRMTGIGV
GVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRVGGMITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAG
ILMVDYFLIRGTRLDTRELFDEHGEYSYTGGWNIGAVVALVIGVLPNLPGFLHTAFPASFPNVPAIFNTLYTYAWFVGLA
LASIVYSAWMKLSKGPSARVASA
>Mature_503_residues
MKQTAQPAEPQFATGVQGSSLYNDDLAPTGIAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEEGMSPWQAVMTVLLGNL
IVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLD
ISLAQLACFLVFWALQIYFIVHGTDSIRWLESWSAPIKIVMCIALVWWATSKAGGLGSMLSAPSQFVPGGKKEGMFWITF
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIIGQSVGLPVPMALLSVISVVVTSATVVIYGKAIWDPIDLTSRMTGIGV
GVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRVGGMITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAG
ILMVDYFLIRGTRLDTRELFDEHGEYSYTGGWNIGAVVALVIGVLPNLPGFLHTAFPASFPNVPAIFNTLYTYAWFVGLA
LASIVYSAWMKLSKGPSARVASA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=485, Percent_Identity=28.4536082474227, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322219, Length=456, Percent_Identity=28.5087719298246, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319429, Length=504, Percent_Identity=27.7777777777778, Blast_Score=193, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6319495, Length=493, Percent_Identity=26.369168356998, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6324645, Length=447, Percent_Identity=24.3847874720358, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324766, Length=452, Percent_Identity=22.787610619469, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323266, Length=444, Percent_Identity=22.972972972973, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 54300; Mature: 54300

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTAQPAEPQFATGVQGSSLYNDDLAPTGIAQRTWKWYHFAALWVGMVMNIASYMLAAG
CCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTEEGMSPWQAVMTVLLGNLIVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHH
AIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLDISLAQLACFLVFWALQIYFI
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
VHGTDSIRWLESWSAPIKIVMCIALVWWATSKAGGLGSMLSAPSQFVPGGKKEGMFWITF
EECCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCEEEEEE
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIIGQSVGLPVPMALLSVISVVVTSATVVI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEE
YGKAIWDPIDLTSRMTGIGVGVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRVG
EEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHC
GMITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGILMVDYFLIRGTRLDTRELF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DEHGEYSYTGGWNIGAVVALVIGVLPNLPGFLHTAFPASFPNVPAIFNTLYTYAWFVGLA
HHCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LASIVYSAWMKLSKGPSARVASA
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKQTAQPAEPQFATGVQGSSLYNDDLAPTGIAQRTWKWYHFAALWVGMVMNIASYMLAAG
CCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTEEGMSPWQAVMTVLLGNLIVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHH
AIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLDISLAQLACFLVFWALQIYFI
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
VHGTDSIRWLESWSAPIKIVMCIALVWWATSKAGGLGSMLSAPSQFVPGGKKEGMFWITF
EECCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCEEEEEE
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIIGQSVGLPVPMALLSVISVVVTSATVVI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEE
YGKAIWDPIDLTSRMTGIGVGVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRVG
EEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHC
GMITATIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGILMVDYFLIRGTRLDTRELF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DEHGEYSYTGGWNIGAVVALVIGVLPNLPGFLHTAFPASFPNVPAIFNTLYTYAWFVGLA
HHCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LASIVYSAWMKLSKGPSARVASA
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]