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The map label for this gene is tcyA [H]

Identifier: 91784368

GI number: 91784368

Start: 3333831

End: 3334613

Strand: Reverse

Name: tcyA [H]

Synonym: Bxe_A1432

Alternate gene names: 91784368

Gene position: 3334613-3333831 (Counterclockwise)

Preceding gene: 91784369

Following gene: 91784367

Centisome position: 68.11

GC content: 59.13

Gene sequence:

>783_bases
ATGAAATCGATTCGTTCCATTCTGCTGATCGCGTTGTTCCAGGCGGTGGCCGTGAGCTCCGCATTCGCCGCCGATGAACT
CGCGCAGATCAAATCCGCCGGCGTGTTCAGGATCGGCACCGAAGGCACCTATGCGCCGTTCACGTATCACGACGAATCCG
GCAAGCTGACCGGCTTCGACGTGGAAATCGGCACGGCGATCGCGCAGCGTCTCGGCGTCAAGCCGCAATTCGTCGAAGGC
AAATGGGACGGTCTGATCGCCGGTCTCGACGTGAACCGTTACGACGCGGTGATCAACGAAGTGGCCATCACGGACGCACG
CAAGGTCAAATACGATTTCTCCGACCCGTACATCACTTCGCACGCCGCGTTGATCGTGCAGTCGAACAACACCACGATCA
GGAATTTCGACGACCTGAAGGGCAAGAAGTCGGCCAACACGCTGACCAGCAACTTCGGCAAGATCGCGGCCGCGCACGGC
GCGGAAGTGATTCCCGTGCAGGGCTTCAATGAATCGATCGACCTGCTGACCTCGGGCCGCGTGGATGCCACCGTCAACGA
CTCGCTGTCGTTCCTCGACTTCAAGAAGCACAAGCCGGACGCAAAGGTGAAGATCGCCGCGCTCGATACGTCGAGCGACA
GCAGCGACAAATCCGCCGTGCTGATCAGGAAGGGCAACCCTGATTTGCAGGCGGCGATCAACAAGGCGCTCGCCGACATC
AGGAAAGACGGCACCTACGCGAAGATTTCGCAGAAGTATTTCGGCAAGGACGTCTCCCAGTAA

Upstream 100 bases:

>100_bases
ATTTGCTACAGTCCGCCCACTCAGCCGGGCTCAAACGCCGGCGGGGACTGTCCAACACAAGACCAAGCCACCGACGTGGC
CTATTTGACGGAGCCGCCTG

Downstream 100 bases:

>100_bases
ACGCCTTTCCGCGAGTCTGAATCATGCCGGCATGGTTGCATCTGATGGCGCAGTCGCTGTGGCCCCTGCTGTATGCGGGG
CTCGTGTTCACCGTGCCGCT

Product: ABC polar amino acid transporter, periplasmic ligand binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKSIRSILLIALFQAVAVSSAFAADELAQIKSAGVFRIGTEGTYAPFTYHDESGKLTGFDVEIGTAIAQRLGVKPQFVEG
KWDGLIAGLDVNRYDAVINEVAITDARKVKYDFSDPYITSHAALIVQSNNTTIRNFDDLKGKKSANTLTSNFGKIAAAHG
AEVIPVQGFNESIDLLTSGRVDATVNDSLSFLDFKKHKPDAKVKIAALDTSSDSSDKSAVLIRKGNPDLQAAINKALADI
RKDGTYAKISQKYFGKDVSQ

Sequences:

>Translated_260_residues
MKSIRSILLIALFQAVAVSSAFAADELAQIKSAGVFRIGTEGTYAPFTYHDESGKLTGFDVEIGTAIAQRLGVKPQFVEG
KWDGLIAGLDVNRYDAVINEVAITDARKVKYDFSDPYITSHAALIVQSNNTTIRNFDDLKGKKSANTLTSNFGKIAAAHG
AEVIPVQGFNESIDLLTSGRVDATVNDSLSFLDFKKHKPDAKVKIAALDTSSDSSDKSAVLIRKGNPDLQAAINKALADI
RKDGTYAKISQKYFGKDVSQ
>Mature_260_residues
MKSIRSILLIALFQAVAVSSAFAADELAQIKSAGVFRIGTEGTYAPFTYHDESGKLTGFDVEIGTAIAQRLGVKPQFVEG
KWDGLIAGLDVNRYDAVINEVAITDARKVKYDFSDPYITSHAALIVQSNNTTIRNFDDLKGKKSANTLTSNFGKIAAAHG
AEVIPVQGFNESIDLLTSGRVDATVNDSLSFLDFKKHKPDAKVKIAALDTSSDSSDKSAVLIRKGNPDLQAAINKALADI
RKDGTYAKISQKYFGKDVSQ

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=38.9312977099237, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=32.9113924050633, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=31.3953488372093, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787088, Length=259, Percent_Identity=27.4131274131274, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787031, Length=260, Percent_Identity=28.8461538461538, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787085, Length=219, Percent_Identity=27.8538812785388, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28009; Mature: 28009

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIRSILLIALFQAVAVSSAFAADELAQIKSAGVFRIGTEGTYAPFTYHDESGKLTGFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEEE
VEIGTAIAQRLGVKPQFVEGKWDGLIAGLDVNRYDAVINEVAITDARKVKYDFSDPYITS
EHHHHHHHHHHCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEC
HAALIVQSNNTTIRNFDDLKGKKSANTLTSNFGKIAAAHGAEVIPVQGFNESIDLLTSGR
CEEEEEECCCCEECCCHHCCCCCCCHHHHHCCCCEEECCCCEEEEECCCCCCCCEEECCC
VDATVNDSLSFLDFKKHKPDAKVKIAALDTSSDSSDKSAVLIRKGNPDLQAAINKALADI
CCEEECCCEEEEEHHCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
RKDGTYAKISQKYFGKDVSQ
HCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSIRSILLIALFQAVAVSSAFAADELAQIKSAGVFRIGTEGTYAPFTYHDESGKLTGFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCEEEEE
VEIGTAIAQRLGVKPQFVEGKWDGLIAGLDVNRYDAVINEVAITDARKVKYDFSDPYITS
EHHHHHHHHHHCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEC
HAALIVQSNNTTIRNFDDLKGKKSANTLTSNFGKIAAAHGAEVIPVQGFNESIDLLTSGR
CEEEEEECCCCEECCCHHCCCCCCCHHHHHCCCCEEECCCCEEEEECCCCCCCCEEECCC
VDATVNDSLSFLDFKKHKPDAKVKIAALDTSSDSSDKSAVLIRKGNPDLQAAINKALADI
CCEEECCCEEEEEHHCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
RKDGTYAKISQKYFGKDVSQ
HCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]