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The map label for this gene is yiaO [H]

Identifier: 91784325

GI number: 91784325

Start: 3286375

End: 3287409

Strand: Reverse

Name: yiaO [H]

Synonym: Bxe_A1475

Alternate gene names: 91784325

Gene position: 3287409-3286375 (Counterclockwise)

Preceding gene: 91784326

Following gene: 91784324

Centisome position: 67.15

GC content: 62.03

Gene sequence:

>1035_bases
ATGCTGAAGAAAACCTGTCGTACCGGCGTTGCAGCGATCGCTTTCGTGCTGTCCTCCCTTGCCGCGTTGCCGGCGTCCGC
GCAGGACACGTGGCGCATGGCTACCGATCAGCAGGCCGATAGCCCCGAAGGCAAGGCGTATCAGCGTTTCGCCGATCTCG
TGGCGAAGTACTCGAACGATCAGCTGAAGATAACGATCTTTCCGGCCTCGCAACTCGGCAGCGCGAGTGCCATGCTCGAA
CAGATCAGCGGCGGATTGATCCAGGTGTTTCCGGACGCGCTTGCGGATGCGTCGAAATGGGTACCCGACGTGCAGTACGT
CAGCTCGCCGTTTCTGTTCCAGAGCCGCGCACAGTGGGTCAACTTCATGCACTCCGACGTCGTGAAGGGATGGGTGGGCC
AGATTCATGACAAGGCAGGCGTGGATATTCTCGGCGATCCGTCGCAATTCATGCGCGGCCCGTATCGCGTGCTCGTGTCG
ACGAAGCCGGTGCACAGCCTCGCCGACATCGGCGGTCTGAAGCTGCGCCTTGCGAAGGACAAGCTGAGCGTCGACGTGTG
GACGAGACTCGGCGCGAGTGTGCAGGTGCTGGACTGGTCCGCAGTGTACGACGGCCTCGACCGTCACATGATCGACGCGA
TGACGAGTCCGATTTCCCAGCTCGAAAGCATCAAGGTGACCGAGGTGGCCAAGTATGTTGCACGCACCGACGAGTACTGG
CAGAGCGTTGCGTTCTTCGTCAACCACAAGGCGTATGCGGCGCTCTCGCCGGCCGAGAAGAGCGCTGTGGACCGCGCCTA
TGCCGAGAGCAGCGCGTACGCGGCGGAACTGGAAAAGAGCTTCACCGAAACGGCCATCGACAAGATGAAGAAGAGCGGCG
TGCAGTTCACCGTGCTCGACACGAAGCCGATCGTGCAGCAGGCGCGCACGCTGTACGACGCGCGCTCGACAGCCGGCACC
TTGCCGAAGGGCTTTCTCGCTGCGGTGCAGTCGGTGCAGTCGAACGTGACGACGGCATCGGCGTCAGGTCAGTAG

Upstream 100 bases:

>100_bases
TGTCGGGCCCGAACGTATTGCATTGAACCAAGGGATGGGCTGCGTGCGACGCGAGAGTTGCATCAGGTCCGCGAGACAAC
CTGTTTTGGGTGAGAGAGAA

Downstream 100 bases:

>100_bases
CACGGTCAAGATTAGTGGGAGAAGGATGATATGCACAGGCTCACGGGTTACATCGACCGGCTGCTGGATTTTTTCTGCAG
TGTCTTGCGGGTGGTCGCCG

Product: TRAP dicarboxylate transporter, periplasmic ligand binding subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLKKTCRTGVAAIAFVLSSLAALPASAQDTWRMATDQQADSPEGKAYQRFADLVAKYSNDQLKITIFPASQLGSASAMLE
QISGGLIQVFPDALADASKWVPDVQYVSSPFLFQSRAQWVNFMHSDVVKGWVGQIHDKAGVDILGDPSQFMRGPYRVLVS
TKPVHSLADIGGLKLRLAKDKLSVDVWTRLGASVQVLDWSAVYDGLDRHMIDAMTSPISQLESIKVTEVAKYVARTDEYW
QSVAFFVNHKAYAALSPAEKSAVDRAYAESSAYAAELEKSFTETAIDKMKKSGVQFTVLDTKPIVQQARTLYDARSTAGT
LPKGFLAAVQSVQSNVTTASASGQ

Sequences:

>Translated_344_residues
MLKKTCRTGVAAIAFVLSSLAALPASAQDTWRMATDQQADSPEGKAYQRFADLVAKYSNDQLKITIFPASQLGSASAMLE
QISGGLIQVFPDALADASKWVPDVQYVSSPFLFQSRAQWVNFMHSDVVKGWVGQIHDKAGVDILGDPSQFMRGPYRVLVS
TKPVHSLADIGGLKLRLAKDKLSVDVWTRLGASVQVLDWSAVYDGLDRHMIDAMTSPISQLESIKVTEVAKYVARTDEYW
QSVAFFVNHKAYAALSPAEKSAVDRAYAESSAYAAELEKSFTETAIDKMKKSGVQFTVLDTKPIVQQARTLYDARSTAGT
LPKGFLAAVQSVQSNVTTASASGQ
>Mature_344_residues
MLKKTCRTGVAAIAFVLSSLAALPASAQDTWRMATDQQADSPEGKAYQRFADLVAKYSNDQLKITIFPASQLGSASAMLE
QISGGLIQVFPDALADASKWVPDVQYVSSPFLFQSRAQWVNFMHSDVVKGWVGQIHDKAGVDILGDPSQFMRGPYRVLVS
TKPVHSLADIGGLKLRLAKDKLSVDVWTRLGASVQVLDWSAVYDGLDRHMIDAMTSPISQLESIKVTEVAKYVARTDEYW
QSVAFFVNHKAYAALSPAEKSAVDRAYAESSAYAAELEKSFTETAIDKMKKSGVQFTVLDTKPIVQQARTLYDARSTAGT
LPKGFLAAVQSVQSNVTTASASGQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=329, Percent_Identity=27.0516717325228, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37387; Mature: 37387

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTCRTGVAAIAFVLSSLAALPASAQDTWRMATDQQADSPEGKAYQRFADLVAKYSND
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
QLKITIFPASQLGSASAMLEQISGGLIQVFPDALADASKWVPDVQYVSSPFLFQSRAQWV
EEEEEEECCHHCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
NFMHSDVVKGWVGQIHDKAGVDILGDPSQFMRGPYRVLVSTKPVHSLADIGGLKLRLAKD
HHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCCCEEEEEECCCHHHHHHCCCEEEEEEHH
KLSVDVWTRLGASVQVLDWSAVYDGLDRHMIDAMTSPISQLESIKVTEVAKYVARTDEYW
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSVAFFVNHKAYAALSPAEKSAVDRAYAESSAYAAELEKSFTETAIDKMKKSGVQFTVLD
HHHHHHHCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TKPIVQQARTLYDARSTAGTLPKGFLAAVQSVQSNVTTASASGQ
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLKKTCRTGVAAIAFVLSSLAALPASAQDTWRMATDQQADSPEGKAYQRFADLVAKYSND
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
QLKITIFPASQLGSASAMLEQISGGLIQVFPDALADASKWVPDVQYVSSPFLFQSRAQWV
EEEEEEECCHHCCHHHHHHHHHCCCCEEECHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
NFMHSDVVKGWVGQIHDKAGVDILGDPSQFMRGPYRVLVSTKPVHSLADIGGLKLRLAKD
HHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCCCEEEEEECCCHHHHHHCCCEEEEEEHH
KLSVDVWTRLGASVQVLDWSAVYDGLDRHMIDAMTSPISQLESIKVTEVAKYVARTDEYW
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSVAFFVNHKAYAALSPAEKSAVDRAYAESSAYAAELEKSFTETAIDKMKKSGVQFTVLD
HHHHHHHCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TKPIVQQARTLYDARSTAGTLPKGFLAAVQSVQSNVTTASASGQ
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]