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The map label for this gene is glnG [H]

Identifier: 91784276

GI number: 91784276

Start: 3231841

End: 3233358

Strand: Reverse

Name: glnG [H]

Synonym: Bxe_A1524

Alternate gene names: 91784276

Gene position: 3233358-3231841 (Counterclockwise)

Preceding gene: 91784277

Following gene: 91784275

Centisome position: 66.04

GC content: 64.76

Gene sequence:

>1518_bases
ATGAAGCCGATCTGGATAGTAGACGACGATCAATCGATTCGCTGGGTGCTCGAAAAAGCACTGGCCCGCGAAAACTTCGC
GACCCGCAGCTTCGCGAACGTGCGCGAGGCGTCGGCCGCGCTCGATCACGACAGTCCGCAGGTGCTGGTGTCCGACATCA
GGATGCCCGGCGGCTCCGGACTCGAATTGCTGCAAACCGTTCGTGACAAGGTGCCGGGCTTGCCCGTCATCATCATGACG
GCGTTCTCGGATCTGGACAGCGCGGTCGCCGCATTCCAGGGCGGCGCGTTCGAATACCTCGCCAAGCCGTTCGACGTCGA
CAAGGCGGTCGAACTGATTCGCCGCGCGGTGGACGAAAGCATGCGCGGCGAACAGACGTGGGACGAGCGCGTTACCGAAG
CACCGGAAATGCTCGGCCAGGCGCCGGCCATGCAGGACATGTTCCGTGCGATCGGCCGTTTGTCCCATTCTGCGGCAACC
GTGCTCATTACCGGCGAATCAGGCACCGGGAAGGAACTGGTCGCGCGTGCGTTGCACCGACATAGCCCACGCGCGAACGG
TCCCTTCATCGCGCTGAACACCGCGGCGATTCCGAAGGATCTGTTGGAATCCGAATTGTTCGGCCATGAGCGCGGCGCCT
TCACCGGCGCACAGGCCATGCGCCAGGGGCGTTTCGAGCAGGCCGAGAACGGCACGCTGTTTCTCGACGAAATCGGCGAC
ATGCCGTTCGATTTGCAGACGCGTCTGCTGCGCGTGCTGTCGGACGGACAGTTCTATCGCGTCGGCGGACACAATCCGTT
GCGCGCCAACGTGCGCGTGATCGCGGCGACGCACCAGAATCTCGAATCGCGCGTACGTCAGGGGCTGTTCCGTGAGGACT
TGTATCACCGCCTCAATGTGATCCGCTTGCGTTTGCCCGCCTTGCGCGAGCGCAGCGAAGACATTCCGCTGCTCACGCGT
CACTTCCTGCAAAAGAGCGCGCGTGATCTCGGCGTCGAACCGAAGCGCGTGTCTGAAGAAGCGCTGGCCTATCTCGCGTC
GCTGCCGTTTCCGGGCAACGTGCGTCAGTTGGAGAACCTCGCCAATTGGCTCACGGTGATGGCGCCCGCGCAGACCATCG
AGATCAAGGACCTGCCGCCCGACCTCGGGCCCGCGCAAGCCGGCGTGAGCGATCTGACCGGCGGCGGCAGCGGGGTGATC
GGTACGGGTGAGGCGGGTCTCGCCGGCGGCGCGCCGGTCAACGGCACGAGTGCCGCGGTGCATCCGGCCGCAGCGGGCGG
CATGCCGGTCGCGACCGCCGTCAGTGCCTGGGAAGGCGGCTTGCGTACCGAAGTCGCGCGCATGTTGCGCGAAAACGCGG
CGGACGTGATGGACGAACTCGCGCGCCGCTTCGAAGCGGCGGTGATTCGCGAAGCGCTCGATTTCACGCGCGGCCGCAAG
GTGGAAGCGGCGGAACGTCTCGGCATTGGCCGCAATACGATTACGCGCAAGATTCAGGAATTGAATCTCGAGCCGTGA

Upstream 100 bases:

>100_bases
TGATCGAAGTGGATAGCCGCCCGGGTCACACCGAGTTTCAGATTCTGCTGCCGCTCGACTGCTAACCCCACGCGTATTTG
AAGACTTCTGACCGACCTAT

Downstream 100 bases:

>100_bases
AGGCATTGCCGGGCTGGCAATGCTGTTTTCTCTGATCCCGATGCTTCGCACCCTGTGTGTCCGGCACGCATGGTGCGGCA
TGGTGTCACCTTAACGAGGC

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREASAALDHDSPQVLVSDIRMPGGSGLELLQTVRDKVPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDESMRGEQTWDERVTEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTR
HFLQKSARDLGVEPKRVSEEALAYLASLPFPGNVRQLENLANWLTVMAPAQTIEIKDLPPDLGPAQAGVSDLTGGGSGVI
GTGEAGLAGGAPVNGTSAAVHPAAAGGMPVATAVSAWEGGLRTEVARMLRENAADVMDELARRFEAAVIREALDFTRGRK
VEAAERLGIGRNTITRKIQELNLEP

Sequences:

>Translated_505_residues
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREASAALDHDSPQVLVSDIRMPGGSGLELLQTVRDKVPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDESMRGEQTWDERVTEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTR
HFLQKSARDLGVEPKRVSEEALAYLASLPFPGNVRQLENLANWLTVMAPAQTIEIKDLPPDLGPAQAGVSDLTGGGSGVI
GTGEAGLAGGAPVNGTSAAVHPAAAGGMPVATAVSAWEGGLRTEVARMLRENAADVMDELARRFEAAVIREALDFTRGRK
VEAAERLGIGRNTITRKIQELNLEP
>Mature_505_residues
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREASAALDHDSPQVLVSDIRMPGGSGLELLQTVRDKVPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDESMRGEQTWDERVTEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTR
HFLQKSARDLGVEPKRVSEEALAYLASLPFPGNVRQLENLANWLTVMAPAQTIEIKDLPPDLGPAQAGVSDLTGGGSGVI
GTGEAGLAGGAPVNGTSAAVHPAAAGGMPVATAVSAWEGGLRTEVARMLRENAADVMDELARRFEAAVIREALDFTRGRK
VEAAERLGIGRNTITRKIQELNLEP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=502, Percent_Identity=54.7808764940239, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1788550, Length=512, Percent_Identity=38.671875, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1790437, Length=377, Percent_Identity=42.1750663129974, Blast_Score=288, Evalue=8e-79,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=42.1621621621622, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=52.8888888888889, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789087, Length=287, Percent_Identity=44.9477351916376, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789233, Length=370, Percent_Identity=38.3783783783784, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082152, Length=248, Percent_Identity=47.5806451612903, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=46.5648854961832, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787583, Length=251, Percent_Identity=41.8326693227092, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=45.575221238938, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081858, Length=231, Percent_Identity=34.1991341991342, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789809, Length=111, Percent_Identity=31.5315315315315, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55000; Mature: 55000

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREASAALDHDSPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQTVRDKVPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
MRGEQTWDERVTEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IRLRLPALRERSEDIPLLTRHFLQKSARDLGVEPKRVSEEALAYLASLPFPGNVRQLENL
HHHHCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
ANWLTVMAPAQTIEIKDLPPDLGPAQAGVSDLTGGGSGVIGTGEAGLAGGAPVNGTSAAV
HHHHHHHCCCCEEEEECCCCCCCCHHCCHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCC
HPAAAGGMPVATAVSAWEGGLRTEVARMLRENAADVMDELARRFEAAVIREALDFTRGRK
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEAAERLGIGRNTITRKIQELNLEP
CHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARENFATRSFANVREASAALDHDSPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQTVRDKVPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVDES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
MRGEQTWDERVTEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQAMRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IRLRLPALRERSEDIPLLTRHFLQKSARDLGVEPKRVSEEALAYLASLPFPGNVRQLENL
HHHHCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
ANWLTVMAPAQTIEIKDLPPDLGPAQAGVSDLTGGGSGVIGTGEAGLAGGAPVNGTSAAV
HHHHHHHCCCCEEEEECCCCCCCCHHCCHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCC
HPAAAGGMPVATAVSAWEGGLRTEVARMLRENAADVMDELARRFEAAVIREALDFTRGRK
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEAAERLGIGRNTITRKIQELNLEP
CHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]