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The map label for this gene is ntrB [H]

Identifier: 91784277

GI number: 91784277

Start: 3233394

End: 3234536

Strand: Reverse

Name: ntrB [H]

Synonym: Bxe_A1523

Alternate gene names: 91784277

Gene position: 3234536-3233394 (Counterclockwise)

Preceding gene: 91784278

Following gene: 91784276

Centisome position: 66.07

GC content: 60.72

Gene sequence:

>1143_bases
ATGGTTCTTAAGAATCTGATCAAGGCAAGGAAAACGCACGAGCCGACACTCTCGGACGACGCCCAACTCGTCAGCTCCGG
TTTGCTGCCGGGCTTCGAGGCGTTGCCGACAGTCGTGCTGGTGCTCGAAAAGCGCACGCTGCGCGTCGCGTTTGCGAACC
CGTCGGCGGAGTCGATGCTGGAGCTCTCGCGCCGGCAACTGACGCAAATGGCGTGGCCGCACATCTTCTCGAATGCCGAC
GAACTGGTCGCGACCATTTCCGCGATCGCCGCTCACCGCTTTCATGCCACGCATCTGGACGCCGTGCTCGAACGTCCGGG
CCACGAACCGCTGCATGTGCACGCGATCGTGGGTTTTCTGGAAAGCGCGCAAGACTATGTGCTGCTCGAACTGTTCGAGA
ACGAACGGCATTTACGCACCGACCGCGAGGAGCGCATCAACGACCTCACGGCGGTCAACAAGCAACTGATCCGCAATCTC
GCGCACGAGATCAAGAACCCGCTCGGCGGGATTCGCGGCGCGGCGCAACTGCTCGAGTTCGAACTCGGCGAGCGTCAGCG
TGACGAACTGCGCGAGTACACGCAGGTGATCATCAAGGAATCGGACCGGCTGCAAACGCTGGTTGACCGTTTGCTCGAAC
CGCACCGTCATCCGCATATCGTCGGCGACGTGAATATTCACGAAGTGTGCGAACGCGTGCGCCAGGTGATTCTCGCGGAG
TTTCCGCGCGGCCTCACCATCGAGCGCGATTACGACGTGAGCGTGCCGGACCTGCGCGGCGACAAGGAGCAACTGATCCA
GGCGCTCCTGAACATCGTGCGCAACGCGGCCGAAGCGTTGCGCGAACGTATTTCGCAAGGCGATGCGCGGATCGAATTGC
GCACCCGCGTTGCCCGCAAGATTACGGTGTCGAAACGCTTATGTAAGCTGGCATTGGACTTGCATATCACAGACAACGGC
CCGGGCATTCCCGAAGAAATCCGTGACCGCATTTTCTATCCGCTCGTGTCGGGGCGCGAGGACGGTAGCGGTCTCGGACT
GACGCTTGCGCAGACTTTCGTGCAACAACACGACGGTCTGATCGAAGTGGATAGCCGCCCGGGTCACACCGAGTTTCAGA
TTCTGCTGCCGCTCGACTGCTAA

Upstream 100 bases:

>100_bases
AGGCCGAGCACAACGTTTGCGTCGTATTGTTATCGTTATCAAGCAGGACAGCAGGCGGACTACCTGATTTATCACCATCT
CATATCGGCCAGACTGCAAG

Downstream 100 bases:

>100_bases
CCCCACGCGTATTTGAAGACTTCTGACCGACCTATATGAAGCCGATCTGGATAGTAGACGACGATCAATCGATTCGCTGG
GTGCTCGAAAAAGCACTGGC

Product: putative nitrogen regulation, histidine kinase, protein NR(II)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVLKNLIKARKTHEPTLSDDAQLVSSGLLPGFEALPTVVLVLEKRTLRVAFANPSAESMLELSRRQLTQMAWPHIFSNAD
ELVATISAIAAHRFHATHLDAVLERPGHEPLHVHAIVGFLESAQDYVLLELFENERHLRTDREERINDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERQRDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRQVILAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARKITVSKRLCKLALDLHITDNG
PGIPEEIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGLIEVDSRPGHTEFQILLPLDC

Sequences:

>Translated_380_residues
MVLKNLIKARKTHEPTLSDDAQLVSSGLLPGFEALPTVVLVLEKRTLRVAFANPSAESMLELSRRQLTQMAWPHIFSNAD
ELVATISAIAAHRFHATHLDAVLERPGHEPLHVHAIVGFLESAQDYVLLELFENERHLRTDREERINDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERQRDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRQVILAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARKITVSKRLCKLALDLHITDNG
PGIPEEIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGLIEVDSRPGHTEFQILLPLDC
>Mature_380_residues
MVLKNLIKARKTHEPTLSDDAQLVSSGLLPGFEALPTVVLVLEKRTLRVAFANPSAESMLELSRRQLTQMAWPHIFSNAD
ELVATISAIAAHRFHATHLDAVLERPGHEPLHVHAIVGFLESAQDYVLLELFENERHLRTDREERINDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERQRDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRQVILAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARKITVSKRLCKLALDLHITDNG
PGIPEEIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGLIEVDSRPGHTEFQILLPLDC

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=338, Percent_Identity=41.7159763313609, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1790436, Length=261, Percent_Identity=28.3524904214559, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788549, Length=352, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790346, Length=242, Percent_Identity=26.0330578512397, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43053; Mature: 43053

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKNLIKARKTHEPTLSDDAQLVSSGLLPGFEALPTVVLVLEKRTLRVAFANPSAESML
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHH
ELSRRQLTQMAWPHIFSNADELVATISAIAAHRFHATHLDAVLERPGHEPLHVHAIVGFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ESAQDYVLLELFENERHLRTDREERINDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERQRDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRQVILAE
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
ITVSKRLCKLALDLHITDNGPGIPEEIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGL
HHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCE
IEVDSRPGHTEFQILLPLDC
EEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVLKNLIKARKTHEPTLSDDAQLVSSGLLPGFEALPTVVLVLEKRTLRVAFANPSAESML
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHH
ELSRRQLTQMAWPHIFSNADELVATISAIAAHRFHATHLDAVLERPGHEPLHVHAIVGFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ESAQDYVLLELFENERHLRTDREERINDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERQRDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRQVILAE
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAEALRERISQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
ITVSKRLCKLALDLHITDNGPGIPEEIRDRIFYPLVSGREDGSGLGLTLAQTFVQQHDGL
HHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCE
IEVDSRPGHTEFQILLPLDC
EEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]