Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 91784259

GI number: 91784259

Start: 3207104

End: 3208771

Strand: Reverse

Name: pdhB [H]

Synonym: Bxe_A1542

Alternate gene names: 91784259

Gene position: 3208771-3207104 (Counterclockwise)

Preceding gene: 91784260

Following gene: 91784258

Centisome position: 65.54

GC content: 63.79

Gene sequence:

>1668_bases
ATGAGTCAAGCGATCGAAGTCAAGGTGCCGGACATCGGCGATTACAAGGACATTCCTGTGATCGAGGTGCTGGTGAAGGC
GGGTGACACCGTCGAGAAAGAGCAGTCGCTCGTTACGCTGGAGTCCGACAAGGCGACCATGGACGTGCCGAGCTCGGCCG
CCGGCGTCGTCAAGGAAGTGAAGGTCAAGGTCGGCGACAATGTGTCGGAAGGTTCGCTGATCGTCGTGCTGGAAGGCGCT
GAAGGCGGCGCGGCCGCGCCGGCACCGGCGCCTGCGGCCGCACCGGCAGCGGCTCCTGCGCCGGCACCGGCTGCGGCCCC
CGCTGCTGCGCCGGCGCCCGCGCCCGCCGCGAGCGGCGGCGGTCTGCAGGAAGTCAAGGTGCCGGATATCGGCGACTACA
AAGACATTCCCGTGATCGAAGTCGCGGTGAAGGTCGGTGATCGTGTCGAGAAAGAGCAGTCGCTAGTGACGCTCGAATCC
GACAAGGCGACCATGGACGTGCCGAGCTCGGCCGCCGGCGTCGTCAAGGAAGTGAAGGTCAAGGTCGGCGATACCGTGTC
GGAAGGCTCGGTGATCGTCGTGGTGGAAGCGGAAGGCGGCGCGGCCGCGCCGGCACCGGCGCCGAAGCAGCAAGCCGTCG
AGCAGCCGTCGGATGCGCCGGCCACGCCGTCGCCGGCTCCGGCGGCGCCTTCGGCGCTCGCTCAGGCGCCGGTGATTCCG
GCTGGCGAAGGCGGCGCGCGTCACGCGAGCCATGCGTCGCCGTCCGTGCGCAAGTTCGCGCGCGAACTGGGCGTCGACGT
GACGCAGGTGCAGGGTACGGGTCCGAAGGGCCGCATTACGCAAGCCGACGTGACTGCCTTCATCAAGGGCGTCATGACCG
GTCAGCGTGCGGCGCCGGCTGGTGCGGCCGCACCGGCTGCGGCGGGCGGCGGCGAACTGAATCTGCTGCCGTGGCCGAAG
ATCGACTTCACGAAGTTCGGTCCGGTCGATCCGAAGCCGCTGTCGCGCATCAAGAAGATTTCGGGCGCGAATCTGCATCG
CAACTGGGTCATGATTCCGCATGTCACGAACAACGACGAAGCGGACATCACGGAACTCGAAGCGCTGCGCGTGCAACTGA
ACAAGGAAAACGAAAAGGCCGGCGTGAAGATCACGATGCTGGCATTCGTGATCAAGGCGGTTGTTTCGGCTTTGAAGCAG
TTCCCGACGTTCAACGCCAGCCTCGACGGCGACAACCTGGTGTTCAAGCAGTACTTCCATATCGGCTTTGCGGCCGACAC
GCCGAATGGTCTGGTCGTTCCTGTCATTCGCGATGCGGACAAGAAGGGTTTGATCGATATCGCGAGGGAAATGGCCGAGT
TGTCGAAGGCTGCGCGCGACGGCAAGCTGAAGCCGGATCAGATGCAAGGCGGGTGCTTCTCGATCTCTTCTTTGGGTGGC
ATTGGCGGCACGAACTTCACGCCGATTATCAATGCGCCTGAAGTCGCCATTCTTGGTCTGTCGCGCGGTGCGACGAAGCC
GGTTTGGGATGGTAAGCAGTTCGTGCCGCGTTTGATCCTGCCGCTTTCTTTGTCGTATGACCATCGGGTGATCGATGGCG
CTGCGGCGGCCAGGTTCAATGCTTATCTTGGGGCGATTCTTGCCGATTTCCGGCGTGTGATTCTTTGA

Upstream 100 bases:

>100_bases
CGATGACCGTCTAAGGCATCCTTCCCCGTGTGCCACGGCGTGCGCGCCCGCCCCTGTTCGACAGGGGCCGGGTGCGTGCG
CGGCCCAGGAGACACTAACA

Downstream 100 bases:

>100_bases
TGTGGTGATTGGGTTGCGCGGGGCGCGGGTTGTTTTTCTGTGATCCGCGTCTCTCGTTGAATTTGCATCCATGTCGGTCT
ATTAGTGTTGCCCCTGTGCG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVKVKVGDNVSEGSLIVVLEGA
EGGAAAPAPAPAAAPAAAPAPAPAAAPAAAPAPAPAASGGGLQEVKVPDIGDYKDIPVIEVAVKVGDRVEKEQSLVTLES
DKATMDVPSSAAGVVKEVKVKVGDTVSEGSVIVVVEAEGGAAAPAPAPKQQAVEQPSDAPATPSPAPAAPSALAQAPVIP
AGEGGARHASHASPSVRKFARELGVDVTQVQGTGPKGRITQADVTAFIKGVMTGQRAAPAGAAAPAAAGGGELNLLPWPK
IDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKENEKAGVKITMLAFVIKAVVSALKQ
FPTFNASLDGDNLVFKQYFHIGFAADTPNGLVVPVIRDADKKGLIDIAREMAELSKAARDGKLKPDQMQGGCFSISSLGG
IGGTNFTPIINAPEVAILGLSRGATKPVWDGKQFVPRLILPLSLSYDHRVIDGAAAARFNAYLGAILADFRRVIL

Sequences:

>Translated_555_residues
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVKVKVGDNVSEGSLIVVLEGA
EGGAAAPAPAPAAAPAAAPAPAPAAAPAAAPAPAPAASGGGLQEVKVPDIGDYKDIPVIEVAVKVGDRVEKEQSLVTLES
DKATMDVPSSAAGVVKEVKVKVGDTVSEGSVIVVVEAEGGAAAPAPAPKQQAVEQPSDAPATPSPAPAAPSALAQAPVIP
AGEGGARHASHASPSVRKFARELGVDVTQVQGTGPKGRITQADVTAFIKGVMTGQRAAPAGAAAPAAAGGGELNLLPWPK
IDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKENEKAGVKITMLAFVIKAVVSALKQ
FPTFNASLDGDNLVFKQYFHIGFAADTPNGLVVPVIRDADKKGLIDIAREMAELSKAARDGKLKPDQMQGGCFSISSLGG
IGGTNFTPIINAPEVAILGLSRGATKPVWDGKQFVPRLILPLSLSYDHRVIDGAAAARFNAYLGAILADFRRVIL
>Mature_554_residues
SQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEVKVKVGDNVSEGSLIVVLEGAE
GGAAAPAPAPAAAPAAAPAPAPAAAPAAAPAPAPAASGGGLQEVKVPDIGDYKDIPVIEVAVKVGDRVEKEQSLVTLESD
KATMDVPSSAAGVVKEVKVKVGDTVSEGSVIVVVEAEGGAAAPAPAPKQQAVEQPSDAPATPSPAPAAPSALAQAPVIPA
GEGGARHASHASPSVRKFARELGVDVTQVQGTGPKGRITQADVTAFIKGVMTGQRAAPAGAAAPAAAGGGELNLLPWPKI
DFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKENEKAGVKITMLAFVIKAVVSALKQF
PTFNASLDGDNLVFKQYFHIGFAADTPNGLVVPVIRDADKKGLIDIAREMAELSKAARDGKLKPDQMQGGCFSISSLGGI
GGTNFTPIINAPEVAILGLSRGATKPVWDGKQFVPRLILPLSLSYDHRVIDGAAAARFNAYLGAILADFRRVIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=424, Percent_Identity=32.311320754717, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI110671329, Length=446, Percent_Identity=30.4932735426009, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI203098816, Length=480, Percent_Identity=28.125, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI203098753, Length=442, Percent_Identity=28.5067873303167, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=36.0869565217391, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.9084967320261, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786305, Length=559, Percent_Identity=52.2361359570662, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=31.1435523114355, Blast_Score=183, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=30.2107728337237, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=29.0909090909091, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=204, Percent_Identity=38.2352941176471, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=28.9473684210526, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=437, Percent_Identity=29.5194508009153, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859875, Length=445, Percent_Identity=30.1123595505618, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=31.140350877193, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=31.140350877193, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=34.2995169082126, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57042; Mature: 56911

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEV
CCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCHHHHHHHHE
KVKVGDNVSEGSLIVVLEGAEGGAAAPAPAPAAAPAAAPAPAPAAAPAAAPAPAPAASGG
EEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLQEVKVPDIGDYKDIPVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSSAAGVVKEVKV
CCEEEECCCCCCCCCCCCEEEEEHHCCHHHHCCCEEEEECCCCEECCCCHHHHHHHHHHE
KVGDTVSEGSVIVVVEAEGGAAAPAPAPKQQAVEQPSDAPATPSPAPAAPSALAQAPVIP
EECCEECCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCC
AGEGGARHASHASPSVRKFARELGVDVTQVQGTGPKGRITQADVTAFIKGVMTGQRAAPA
CCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCCC
GAAAPAAAGGGELNLLPWPKIDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDE
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCC
ADITELEALRVQLNKENEKAGVKITMLAFVIKAVVSALKQFPTFNASLDGDNLVFKQYFH
CCHHHHEEHHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHEE
IGFAADTPNGLVVPVIRDADKKGLIDIAREMAELSKAARDGKLKPDQMQGGCFSISSLGG
EEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCC
IGGTNFTPIINAPEVAILGLSRGATKPVWDGKQFVPRLILPLSLSYDHRVIDGAAAARFN
CCCCCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHEEEECCCCCEECCCHHHHHHH
AYLGAILADFRRVIL
HHHHHHHHHHHHHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDIPVIEVLVKAGDTVEKEQSLVTLESDKATMDVPSSAAGVVKEV
CCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCHHHHHHHHE
KVKVGDNVSEGSLIVVLEGAEGGAAAPAPAPAAAPAAAPAPAPAAAPAAAPAPAPAASGG
EEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLQEVKVPDIGDYKDIPVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSSAAGVVKEVKV
CCEEEECCCCCCCCCCCCEEEEEHHCCHHHHCCCEEEEECCCCEECCCCHHHHHHHHHHE
KVGDTVSEGSVIVVVEAEGGAAAPAPAPKQQAVEQPSDAPATPSPAPAAPSALAQAPVIP
EECCEECCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCC
AGEGGARHASHASPSVRKFARELGVDVTQVQGTGPKGRITQADVTAFIKGVMTGQRAAPA
CCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCCC
GAAAPAAAGGGELNLLPWPKIDFTKFGPVDPKPLSRIKKISGANLHRNWVMIPHVTNNDE
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCC
ADITELEALRVQLNKENEKAGVKITMLAFVIKAVVSALKQFPTFNASLDGDNLVFKQYFH
CCHHHHEEHHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHEE
IGFAADTPNGLVVPVIRDADKKGLIDIAREMAELSKAARDGKLKPDQMQGGCFSISSLGG
EEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCC
IGGTNFTPIINAPEVAILGLSRGATKPVWDGKQFVPRLILPLSLSYDHRVIDGAAAARFN
CCCCCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHEEEECCCCCEECCCHHHHHHH
AYLGAILADFRRVIL
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]