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The map label for this gene is ispG [H]

Identifier: 91784207

GI number: 91784207

Start: 3151796

End: 3153100

Strand: Reverse

Name: ispG [H]

Synonym: Bxe_A1594

Alternate gene names: 91784207

Gene position: 3153100-3151796 (Counterclockwise)

Preceding gene: 91784208

Following gene: 91784206

Centisome position: 64.4

GC content: 64.14

Gene sequence:

>1305_bases
ATGCATTCCGAAGCTCAATCCCAATCCAGTAGCAAGATCGTTTCCAATGAGCCGGTGTTCGGCGGCCAGGCCGCGCGGCG
CAAGTCGCACGCGGTCGACGTCCGTTGGGGCGGTCAGCTCGTCACGATCGGTGGCGACGCGCCTGTGCGTGTCCAGTCCA
TGACCAACACGGACACCGCGGACGCGATCGGCACCGCAATCCAGATCAAGGAGCTGGCGCAGGCCGGCTCGGAACTGGTG
CGTATCACGGTGAACACGCCGGAAGCGGCTGCGGCCGTGCCGGCCGTGCGCGAGCAGCTCGACCGCATGGGCGTGTCGGT
GCCGCTCGTCGGCGACTTCCATTACAACGGCCATTTGCTGTTGCGCGATTACCCCGCCTGTGCGGAGGCGTTGTCCAAGT
ACCGGATCAACCCGGGCAACGTGGGCCACGGCGCGAAGCGCGACACGCAGTTTGCGCAGATGGTCGAAGCCGCGGCGAAG
TACGACAAACCTGTGCGTATCGGCGTCAACTGGGGCAGTCTCGATCAGGATCTGCTCGCCAAAATGATGGACGAGAACGC
CGCGCGTTCCACGCCGTGGGAAGCGCAAAGCGTCATGTATGAAGCGCTCATCCAGTCGGCGATCGGCTCGGCGGAGCGGG
CGGTCGAACTCGGTCTGTCGCGCAACCAGATCATTCTGTCGTGCAAGGTTAGCGGCGTGCAGGATCTGATCGCCGTGTAT
CGTGAACTCGCGCGCCGTTGCGATTTCGCGCTGCATCTCGGCTTGACCGAGGCCGGCATGGGCTCGAAAGGCATCGTGGC
GTCGACGGCGGCGCTGTCCGTGTTGCTGCAGCAGGGCATCGGCGACACGATCCGCATTTCGCTTACGCCGGAACCGGGCG
GATCGCGCACCGGCGAGGTGATCGTCGGCCAGGAAATCCTGCAGACCATGGGGCTGCGCTCGTTCACGCCGATGGTGATC
GCGTGCCCGGGTTGCGGCCGTACCACCAGCACGCTGTTCCAGGAACTGGCGTCGCAGATCCAGACCTATCTGCGCACGCA
GATGCCGGTGTGGCGCGATCAGTATCCTGGCGTCGAGAAGATGCACGTCGCGGTGATGGGTTGCATCGTCAACGGTCCGG
GCGAGTCGAAGCAGGCCAACATCGGCATTAGCTTGCCGGGTTCGGGTGAGAATCCTGCCGCGCCGGTGTTCATCGACGGC
GAGAAGGTGAAGACGCTGCGCGGCGAAAACATCGCCCAAGAATTCCAGCAAATCGTGAGTGACTACGTCGAGCGCCGCTA
CGGCCGCGCCGAAGCGCTCAATTAA

Upstream 100 bases:

>100_bases
GACAGATACGGTATGCGCCGCGGCCAACCGGTCCGCGGCGCTTTTTCAATTCAGGCGCTACGTCTGTTGTGTGGCGCATG
TGGCGTAAATGGGTTTTTCG

Downstream 100 bases:

>100_bases
ATATCGGCTACCGAAAGACAGATGACTGAACAGAAGAAAAAGCTCGAAAAGCTGTCCGGCGTGAAGGGCATGAACGACAT
CCTTCCGCAGGAAGCCGGGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MHSEAQSQSSSKIVSNEPVFGGQAARRKSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELAQAGSELV
RITVNTPEAAAAVPAVREQLDRMGVSVPLVGDFHYNGHLLLRDYPACAEALSKYRINPGNVGHGAKRDTQFAQMVEAAAK
YDKPVRIGVNWGSLDQDLLAKMMDENAARSTPWEAQSVMYEALIQSAIGSAERAVELGLSRNQIILSCKVSGVQDLIAVY
RELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGGSRTGEVIVGQEILQTMGLRSFTPMVI
ACPGCGRTTSTLFQELASQIQTYLRTQMPVWRDQYPGVEKMHVAVMGCIVNGPGESKQANIGISLPGSGENPAAPVFIDG
EKVKTLRGENIAQEFQQIVSDYVERRYGRAEALN

Sequences:

>Translated_434_residues
MHSEAQSQSSSKIVSNEPVFGGQAARRKSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELAQAGSELV
RITVNTPEAAAAVPAVREQLDRMGVSVPLVGDFHYNGHLLLRDYPACAEALSKYRINPGNVGHGAKRDTQFAQMVEAAAK
YDKPVRIGVNWGSLDQDLLAKMMDENAARSTPWEAQSVMYEALIQSAIGSAERAVELGLSRNQIILSCKVSGVQDLIAVY
RELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGGSRTGEVIVGQEILQTMGLRSFTPMVI
ACPGCGRTTSTLFQELASQIQTYLRTQMPVWRDQYPGVEKMHVAVMGCIVNGPGESKQANIGISLPGSGENPAAPVFIDG
EKVKTLRGENIAQEFQQIVSDYVERRYGRAEALN
>Mature_434_residues
MHSEAQSQSSSKIVSNEPVFGGQAARRKSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELAQAGSELV
RITVNTPEAAAAVPAVREQLDRMGVSVPLVGDFHYNGHLLLRDYPACAEALSKYRINPGNVGHGAKRDTQFAQMVEAAAK
YDKPVRIGVNWGSLDQDLLAKMMDENAARSTPWEAQSVMYEALIQSAIGSAERAVELGLSRNQIILSCKVSGVQDLIAVY
RELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGGSRTGEVIVGQEILQTMGLRSFTPMVI
ACPGCGRTTSTLFQELASQIQTYLRTQMPVWRDQYPGVEKMHVAVMGCIVNGPGESKQANIGISLPGSGENPAAPVFIDG
EKVKTLRGENIAQEFQQIVSDYVERRYGRAEALN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=391, Percent_Identity=34.2710997442455, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46575; Mature: 46575

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSEAQSQSSSKIVSNEPVFGGQAARRKSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTA
CCCCHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCEEEEECCCCCEEEEECCCCCHH
DAIGTAIQIKELAQAGSELVRITVNTPEAAAAVPAVREQLDRMGVSVPLVGDFHYNGHLL
HHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEE
LRDYPACAEALSKYRINPGNVGHGAKRDTQFAQMVEAAAKYDKPVRIGVNWGSLDQDLLA
EECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
KMMDENAARSTPWEAQSVMYEALIQSAIGSAERAVELGLSRNQIILSCKVSGVQDLIAVY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
RELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGGSRTGEV
HHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
IVGQEILQTMGLRSFTPMVIACPGCGRTTSTLFQELASQIQTYLRTQMPVWRDQYPGVEK
EECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHH
MHVAVMGCIVNGPGESKQANIGISLPGSGENPAAPVFIDGEKVKTLRGENIAQEFQQIVS
HHEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHH
DYVERRYGRAEALN
HHHHHHCCCHHCCC
>Mature Secondary Structure
MHSEAQSQSSSKIVSNEPVFGGQAARRKSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTA
CCCCHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCEEEEECCCCCEEEEECCCCCHH
DAIGTAIQIKELAQAGSELVRITVNTPEAAAAVPAVREQLDRMGVSVPLVGDFHYNGHLL
HHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEE
LRDYPACAEALSKYRINPGNVGHGAKRDTQFAQMVEAAAKYDKPVRIGVNWGSLDQDLLA
EECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
KMMDENAARSTPWEAQSVMYEALIQSAIGSAERAVELGLSRNQIILSCKVSGVQDLIAVY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
RELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGGSRTGEV
HHHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
IVGQEILQTMGLRSFTPMVIACPGCGRTTSTLFQELASQIQTYLRTQMPVWRDQYPGVEK
EECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHH
MHVAVMGCIVNGPGESKQANIGISLPGSGENPAAPVFIDGEKVKTLRGENIAQEFQQIVS
HHEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHH
DYVERRYGRAEALN
HHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA