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The map label for this gene is hisS [H]

Identifier: 91784206

GI number: 91784206

Start: 3150434

End: 3151774

Strand: Reverse

Name: hisS [H]

Synonym: Bxe_A1595

Alternate gene names: 91784206

Gene position: 3151774-3150434 (Counterclockwise)

Preceding gene: 91784207

Following gene: 91784205

Centisome position: 64.38

GC content: 58.99

Gene sequence:

>1341_bases
ATGACTGAACAGAAGAAAAAGCTCGAAAAGCTGTCCGGCGTGAAGGGCATGAACGACATCCTTCCGCAGGAAGCCGGGCT
GTGGGAATTTTTTGAAACCACCGTCAAGTCTATGCTGCGTTCGTACGGATACCAGAATATCCGTACGCCGATCATCGAGC
ATACGCAGCTGTTCAAGCGCGGTATCGGTGAGGTGACCGATATCGTCGAGAAAGAGATGTACAGCTTTACCGACGCGCTC
AATGGTGAAAATCTGACCATGCGGCCGGAAAATACGGCAGCGGTGGTGCGCGCGGCGATCGAACACAACATGCTGTACGA
CGGCCCGAAGCGCCTGTGGTACATCGGTCCGATGTTCCGTCACGAGCGTCCGCAGCGCGGCCGCTATCGCCAGTTCCATC
AGGTCGGTGTGGAAGCGCTGGGCTTTGCCGGTCCGGACGCCGACGCTGAAATCATCATGATGTGCCAGCGCCTGTGGGAC
GATCTCGGGCTGATGGGCATCAAGCTCGAACTCAATTCGCTGGGTCTCGCGCACGAACGCGCGGCGCATCGTGTCGAACT
GATCGCGTACCTCGAAAAGCACATGGACGTGCTCGACGAAGAAGCGAAGCGTCGTCTCTACACGAACCCGCTGCGCGTGC
TCGATACGAAGAATCCGGCCATGCAGGAAGTCGCGCAGAACGCGCCGAAGCTGATCGATTTTCTCGGCGAAGAATCGCGC
GCGCACTTCGAAGGTTTGCAGCGCATTCTGAAGGCGAACAATATTCCGTTCACGATCAATCAGCGTCTCGTGCGCGGTCT
CGATTATTACAATCTGACCGTGTTCGAATGGGTGACCGACAAGCTCGGCGCGCAAGGCACCGTTGCGGCCGGCGGCCGTT
ACGATCCGCTGATCGAACAGCTCGGCGGCAAGCCCACGGCGGCATGCGGCTGGGCAATGGGCATCGAGCGGATCCTCGAA
TTGCTGAAAGAAGAGCAACTCGTGCCGGAAGACGAAGGTTGCGACGTGTACGTGGTCCATCAGGGCGAGGCGGCGCGCGA
ACAGGCCTTCATCATTGCCGAGCGTTTGCGCGACACGGGCCTCGACGTCATCCTGCATTGCAGTGCCGACGGTCAGACGG
CCAGCTTCAAATCGCAGATGAAGCGTGCGGATGCAAGCGGCGCGGCCTTTGCGGTGGTGCTCGGTGAAGACGAGATCGCC
AACGGCACGGTCGGCGTGAAACCGTTGCGCGATACGAATGCTGACGGCGGTAAAAACGAGCAACACAACGTGCCCGCCGA
AGACTTGACCGAATTTCTAATCAATGCGATGGTTGCAACCGCCGAAGACGGCGACGACTGA

Upstream 100 bases:

>100_bases
AAACATCGCCCAAGAATTCCAGCAAATCGTGAGTGACTACGTCGAGCGCCGCTACGGCCGCGCCGAAGCGCTCAATTAAA
TATCGGCTACCGAAAGACAG

Downstream 100 bases:

>100_bases
TCGCGATGTCGTCGAGCCGGTTGGTTTCCAATCGGACTCGACACGCACATGTAGCAAGAAAGAGGAAATCGCCGGGCAAT
GAGTTACCACGACGAACAAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTEQKKKLEKLSGVKGMNDILPQEAGLWEFFETTVKSMLRSYGYQNIRTPIIEHTQLFKRGIGEVTDIVEKEMYSFTDAL
NGENLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWD
DLGLMGIKLELNSLGLAHERAAHRVELIAYLEKHMDVLDEEAKRRLYTNPLRVLDTKNPAMQEVAQNAPKLIDFLGEESR
AHFEGLQRILKANNIPFTINQRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILE
LLKEEQLVPEDEGCDVYVVHQGEAAREQAFIIAERLRDTGLDVILHCSADGQTASFKSQMKRADASGAAFAVVLGEDEIA
NGTVGVKPLRDTNADGGKNEQHNVPAEDLTEFLINAMVATAEDGDD

Sequences:

>Translated_446_residues
MTEQKKKLEKLSGVKGMNDILPQEAGLWEFFETTVKSMLRSYGYQNIRTPIIEHTQLFKRGIGEVTDIVEKEMYSFTDAL
NGENLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWD
DLGLMGIKLELNSLGLAHERAAHRVELIAYLEKHMDVLDEEAKRRLYTNPLRVLDTKNPAMQEVAQNAPKLIDFLGEESR
AHFEGLQRILKANNIPFTINQRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILE
LLKEEQLVPEDEGCDVYVVHQGEAAREQAFIIAERLRDTGLDVILHCSADGQTASFKSQMKRADASGAAFAVVLGEDEIA
NGTVGVKPLRDTNADGGKNEQHNVPAEDLTEFLINAMVATAEDGDD
>Mature_445_residues
TEQKKKLEKLSGVKGMNDILPQEAGLWEFFETTVKSMLRSYGYQNIRTPIIEHTQLFKRGIGEVTDIVEKEMYSFTDALN
GENLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWDD
LGLMGIKLELNSLGLAHERAAHRVELIAYLEKHMDVLDEEAKRRLYTNPLRVLDTKNPAMQEVAQNAPKLIDFLGEESRA
HFEGLQRILKANNIPFTINQRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILEL
LKEEQLVPEDEGCDVYVVHQGEAAREQAFIIAERLRDTGLDVILHCSADGQTASFKSQMKRADASGAAFAVVLGEDEIAN
GTVGVKPLRDTNADGGKNEQHNVPAEDLTEFLINAMVATAEDGDD

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=407, Percent_Identity=56.5110565110565, Blast_Score=458, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 49931; Mature: 49800

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQKKKLEKLSGVKGMNDILPQEAGLWEFFETTVKSMLRSYGYQNIRTPIIEHTQLFKR
CCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHH
GIGEVTDIVEKEMYSFTDALNGENLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFR
CCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHH
HERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWDDLGLMGIKLELNSLGLAHER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHCCCHHHH
AAHRVELIAYLEKHMDVLDEEAKRRLYTNPLRVLDTKNPAMQEVAQNAPKLIDFLGEESR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHCCHHHHHHHCCCHH
AHFEGLQRILKANNIPFTINQRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQ
HHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHH
LGGKPTAACGWAMGIERILELLKEEQLVPEDEGCDVYVVHQGEAAREQAFIIAERLRDTG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
LDVILHCSADGQTASFKSQMKRADASGAAFAVVLGEDEIANGTVGVKPLRDTNADGGKNE
CEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
QHNVPAEDLTEFLINAMVATAEDGDD
CCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEQKKKLEKLSGVKGMNDILPQEAGLWEFFETTVKSMLRSYGYQNIRTPIIEHTQLFKR
CCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHH
GIGEVTDIVEKEMYSFTDALNGENLTMRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFR
CCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHH
HERPQRGRYRQFHQVGVEALGFAGPDADAEIIMMCQRLWDDLGLMGIKLELNSLGLAHER
CCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHCCCHHHH
AAHRVELIAYLEKHMDVLDEEAKRRLYTNPLRVLDTKNPAMQEVAQNAPKLIDFLGEESR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHCCHHHHHHHCCCHH
AHFEGLQRILKANNIPFTINQRLVRGLDYYNLTVFEWVTDKLGAQGTVAAGGRYDPLIEQ
HHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHH
LGGKPTAACGWAMGIERILELLKEEQLVPEDEGCDVYVVHQGEAAREQAFIIAERLRDTG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCC
LDVILHCSADGQTASFKSQMKRADASGAAFAVVLGEDEIANGTVGVKPLRDTNADGGKNE
CEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
QHNVPAEDLTEFLINAMVATAEDGDD
CCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA