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The map label for this gene is tilS [H]

Identifier: 91784172

GI number: 91784172

Start: 3110547

End: 3111977

Strand: Reverse

Name: tilS [H]

Synonym: Bxe_A1629

Alternate gene names: 91784172

Gene position: 3111977-3110547 (Counterclockwise)

Preceding gene: 91784173

Following gene: 91784171

Centisome position: 63.56

GC content: 69.18

Gene sequence:

>1431_bases
GTGACTTCCACCGCCGAAACCTCTGCCGACCGCCTCGTTCTCGAGGCGGTCGGCTTTTCGTTGTCCAGGCTTCCTGCAAA
CGCCCGTATTGCGATTGCGTTTAGCGGCGGCGTGGATTCGAGCGTGCTGCTCGATGCCGCGGTGCGTGTCGCGGGCGCGT
CGCGCTGCGTCGCGCTGCATGTGCATCACGGCCTGAGCGCGCATGCGGATGCGTGGCTTGCGCATTGCGAGGCGTTCGCG
CGGGAGCGTGGCGTCGAGTTCGCTGCGGAGCGCGTCGCGGTGTCGCGTGACACGGGTGTGGGCGTCGAGGCGGCCGCGCG
CGATGCGCGTTATCGCGCGCTCGACGACATGTGCGCGTCGCGCGGTGTCGCCACGCTCTGGCTCGCGCAACATGCGGACG
ATCAGGCGGAAACCGTGCTGCTGCAATTGCTGCGGGGCGCCGGCCTCGCGGGTCTGGCCGCGATGGCGCCGAAGTATTTG
CCGGCGAAGGCGTCGGCAACGCGCGTGCGGCCTTTGCTCCATCTGCTGCGTGCGCAACTCGAGCAATACGCGAGCGCGCG
TGCGCTGCGCTGGATCGACGACGAGTCGAACGCGGATACGCGCTATGCGCGCAACGCGTTGCGCCACGACGTGATGCCGG
CGCTCGCTGTCCACTTTCCCGGCTTTCGCGACGCGCTGGCTCGCACGGCGGCACACGCGGCGTCCGCGCAGCGCTTGCTC
GACGAATTCGCGCGCATCGACATGGAAGCCGCATCACGCGACGAAGGACGCGCGCTCTCGCACGACGCCTTGCTCGCGCT
CGACGACGAGCGCGCGCTCAACCTCATGCGCTACTGGATGCGCACGCTGGGCCTCGCCGCCGCGTCGAGCGCGCGTCTTG
GCGATGCGCTGCGGCAACTGCGCGAAATCGGCGCGGCGGGCGAAGGTCATGCGCTGCGTGTCGATCACGCGGGTCATGCA
TTGCGCAGCTATCGCGGCCTCGTGTATTGGGAAGCCGGAGACAGCAGCGGGCCCGCCGACGAAACCGCGCTCGCCGCGCG
CGCGGTCAGCGAGCTGGCATGGCAGGGCGAGTCGGTCTGGCGCTTGCCGCAATGGCGCGGCACTTTCATATTCGCCGACG
CAAGCGCGGGCGAGCCCGACGCGATTCCCGTCAGCGTTCTGCGGCGCGCCGTGTCGAGCGCACGCTCGCGCAGCGGCGGC
GAACGGATGCGCATGGGCGACGCGCACAATGCGCCGGGCCGCACGCTGAAAAACCTGTTCCAGGAACGCGGTATTCCCGC
GTGGAAACGCGACGTGCCGCTGCTCTACATCGGCGAGGAACTGCTGTTCGTGCCGCTGCTCGGGGTGAATCGCGCGGCGT
TGCCGGACCAGCCCGGCGAGGCTGGATCACACGTCAGGATCGTGTGGCGTGAAGATCTGCTGATCGCCTGA

Upstream 100 bases:

>100_bases
CGAACGGTTGATGTCGTACGGCAAGTTCAAGGAAACGACGCCGGGTGCCTAAGCCCGCGCGCGTTTTCTTTCGTCCTTCA
GCGCCCAGCGCGCGAGCGAC

Downstream 100 bases:

>100_bases
CCGGCCGGTCGGACACCCGGATGCGCGAACCGGTGAATCCGGGAAACGTGGCGCAAATCCGCGTGAAACAAGCCTTTGCG
GCCCTTATTGAACGCATTTC

Product: PP family ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTSTAETSADRLVLEAVGFSLSRLPANARIAIAFSGGVDSSVLLDAAVRVAGASRCVALHVHHGLSAHADAWLAHCEAFA
RERGVEFAAERVAVSRDTGVGVEAAARDARYRALDDMCASRGVATLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPKYL
PAKASATRVRPLLHLLRAQLEQYASARALRWIDDESNADTRYARNALRHDVMPALAVHFPGFRDALARTAAHAASAQRLL
DEFARIDMEAASRDEGRALSHDALLALDDERALNLMRYWMRTLGLAAASSARLGDALRQLREIGAAGEGHALRVDHAGHA
LRSYRGLVYWEAGDSSGPADETALAARAVSELAWQGESVWRLPQWRGTFIFADASAGEPDAIPVSVLRRAVSSARSRSGG
ERMRMGDAHNAPGRTLKNLFQERGIPAWKRDVPLLYIGEELLFVPLLGVNRAALPDQPGEAGSHVRIVWREDLLIA

Sequences:

>Translated_476_residues
MTSTAETSADRLVLEAVGFSLSRLPANARIAIAFSGGVDSSVLLDAAVRVAGASRCVALHVHHGLSAHADAWLAHCEAFA
RERGVEFAAERVAVSRDTGVGVEAAARDARYRALDDMCASRGVATLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPKYL
PAKASATRVRPLLHLLRAQLEQYASARALRWIDDESNADTRYARNALRHDVMPALAVHFPGFRDALARTAAHAASAQRLL
DEFARIDMEAASRDEGRALSHDALLALDDERALNLMRYWMRTLGLAAASSARLGDALRQLREIGAAGEGHALRVDHAGHA
LRSYRGLVYWEAGDSSGPADETALAARAVSELAWQGESVWRLPQWRGTFIFADASAGEPDAIPVSVLRRAVSSARSRSGG
ERMRMGDAHNAPGRTLKNLFQERGIPAWKRDVPLLYIGEELLFVPLLGVNRAALPDQPGEAGSHVRIVWREDLLIA
>Mature_475_residues
TSTAETSADRLVLEAVGFSLSRLPANARIAIAFSGGVDSSVLLDAAVRVAGASRCVALHVHHGLSAHADAWLAHCEAFAR
ERGVEFAAERVAVSRDTGVGVEAAARDARYRALDDMCASRGVATLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPKYLP
AKASATRVRPLLHLLRAQLEQYASARALRWIDDESNADTRYARNALRHDVMPALAVHFPGFRDALARTAAHAASAQRLLD
EFARIDMEAASRDEGRALSHDALLALDDERALNLMRYWMRTLGLAAASSARLGDALRQLREIGAAGEGHALRVDHAGHAL
RSYRGLVYWEAGDSSGPADETALAARAVSELAWQGESVWRLPQWRGTFIFADASAGEPDAIPVSVLRRAVSSARSRSGGE
RMRMGDAHNAPGRTLKNLFQERGIPAWKRDVPLLYIGEELLFVPLLGVNRAALPDQPGEAGSHVRIVWREDLLIA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=461, Percent_Identity=36.0086767895879, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51385; Mature: 51254

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTAETSADRLVLEAVGFSLSRLPANARIAIAFSGGVDSSVLLDAAVRVAGASRCVALH
CCCCCCCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
VHHGLSAHADAWLAHCEAFARERGVEFAAERVAVSRDTGVGVEAAARDARYRALDDMCAS
ECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RGVATLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPKYLPAKASATRVRPLLHLLRAQL
CCCCEEHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
EQYASARALRWIDDESNADTRYARNALRHDVMPALAVHFPGFRDALARTAAHAASAQRLL
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DEFARIDMEAASRDEGRALSHDALLALDDERALNLMRYWMRTLGLAAASSARLGDALRQL
HHHHHCCHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
REIGAAGEGHALRVDHAGHALRSYRGLVYWEAGDSSGPADETALAARAVSELAWQGESVW
HHHCCCCCCCEEEECCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
RLPQWRGTFIFADASAGEPDAIPVSVLRRAVSSARSRSGGERMRMGDAHNAPGRTLKNLF
ECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHH
QERGIPAWKRDVPLLYIGEELLFVPLLGVNRAALPDQPGEAGSHVRIVWREDLLIA
HHCCCCCHHHCCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCEECC
>Mature Secondary Structure 
TSTAETSADRLVLEAVGFSLSRLPANARIAIAFSGGVDSSVLLDAAVRVAGASRCVALH
CCCCCCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
VHHGLSAHADAWLAHCEAFARERGVEFAAERVAVSRDTGVGVEAAARDARYRALDDMCAS
ECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RGVATLWLAQHADDQAETVLLQLLRGAGLAGLAAMAPKYLPAKASATRVRPLLHLLRAQL
CCCCEEHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
EQYASARALRWIDDESNADTRYARNALRHDVMPALAVHFPGFRDALARTAAHAASAQRLL
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DEFARIDMEAASRDEGRALSHDALLALDDERALNLMRYWMRTLGLAAASSARLGDALRQL
HHHHHCCHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
REIGAAGEGHALRVDHAGHALRSYRGLVYWEAGDSSGPADETALAARAVSELAWQGESVW
HHHCCCCCCCEEEECCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
RLPQWRGTFIFADASAGEPDAIPVSVLRRAVSSARSRSGGERMRMGDAHNAPGRTLKNLF
ECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHH
QERGIPAWKRDVPLLYIGEELLFVPLLGVNRAALPDQPGEAGSHVRIVWREDLLIA
HHCCCCCHHHCCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA