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The map label for this gene is oprM [H]

Identifier: 91784062

GI number: 91784062

Start: 2986664

End: 2988208

Strand: Reverse

Name: oprM [H]

Synonym: Bxe_A1741

Alternate gene names: 91784062

Gene position: 2988208-2986664 (Counterclockwise)

Preceding gene: 91784063

Following gene: 91784061

Centisome position: 61.04

GC content: 64.27

Gene sequence:

>1545_bases
ATGAGGATGCCGTTTATCGATTCCGAGGCGAGCCGCCGGGAGGTACCGTGGACAACACGGACCCTGCGGCTTGGCAGCTT
GTTCGCTTCCACACTATTCGTGGCAGCCTGCGCCGTCGGCCCCGACTATCACAAGCCACCCCAGGACATCCCTGCTACCT
TTAAGGAAGGCACGGACTGGCAGCGGGCTCAGGTGAACCCGCAGGGCGCTATTTCGAGCACCTGGTGGCTCGACTACCAC
GACGACACGCTGTCGAGCCTCGTGAATCAGGCGCTCAGGGCCAACCAGTCGATCGCCTCTGCGGAAGCCGCATACCGGCT
CGCCAGGGCCACGATCGATGCCAATGTGGCGAGCCTCTTCCCGACAGTCGGGGTGGACGCCTCCGGATCGCGCTCGCGCA
CGGCCGCGGTCGAGAGCGTCACGAGCCAGCCGTACATGAGCAATCTCGCGAGTGTCAGCGCTTCCGCTAGCTGGGAGGTC
GACCTGTGGGGCAGTGTTCGGCGCGAGATCGAGTCGGCCAAAGCCAGCGCTCAGGCAAGCGACGCCCAGCTCGCCGGCGA
ACGCCTCTCGATCGCCGCCAGCGTCGCGGTGGACTATTTCTCGCTGCGTCAGGCCGATGTCGACATCGCTTCACTGAAGC
AGCAGCAGGGCATCGACGCGCGGATTCTGGAGATGGACCGCGCTGGCTTCGTACAGGGAACAGTGTCAAACGACGAAGTC
CTCGAGGCGCAGGACACGCTCGAACTCGTGATCGCTGAGCTGCAAGCCACGGAGACTTCACGCGAGCAGGACGAGCATGC
CATCGCAGTGCTGACCGGTACGCCACCGGCCAGCTTCGCCCTGCCGCCGCAGCCCGGCTATGTCTTCACGACACCCGTCG
TGCCACCGGGATTCCCGTCGCAACTTCTGGAACGGCGCTATGACGTCGTGAGTGCGGAGCGCACGGCCGCCGCGGCCAAC
GCCAGGATCGGTGTAGCAAAGGCCGCGTTCTTCCCGACCCTGACGCTGTCGGCGCAAGGCGGGTTCGAGCATAACGCGCT
GGCGAACCTGTTTTCGGTGCCGAACCGCTTCTGGACACTCGGACCGGATCTCGCCGCCACGATCTTCGACGGCGGCGCGC
GCTCGGCGGCCGTCCACGAAGCCGAGGCCACCTACGACGAACAGATCGCCACATATCGCGGAACCGTGCTCTCGGCGTTC
CAGAGCGTTGAGGATAGCTTATCGTCTTACAACCACCTGTGTAAGCAGGAGCAGGCGTACGCGAACATCTACGAGCGCAA
CCAGCGGCTCTTCTCTAGCCAGCAGGCCCAGATGCGGGCCGGCACGACAAGCGAACAAGCCCTGTTGACCCAACAGATCA
CGTTGCTGCGGGCCGGGCAGAGCCTGAGAGATACGCAGGCGGCATTGACTGAGAGCAGCGTCACGCTAGTCAAGAACCTT
GGCGGCGGATGGCAGTGGGATGACGCCAGGGGTGCGGCAGTCAGCATCAGTGCGAACGCTGCGCAGCAATCCTCGGATCA
AAAATCGGGGGAGAGGTCGCAATGA

Upstream 100 bases:

>100_bases
AGGTGGTTCGTTCAGCTGCATCTCGAACTGATGGGTTACGACTGTATCGACTGAGCGCGATACCTTGAAGCTGCATTGCA
AAGAGAGAAGGAAGCGTGAC

Downstream 100 bases:

>100_bases
AAGATCGCCTTGAACTTAGCCGCTTTGGGGGGCGAAGCATCGAGTCCAGCGATAGCCGGTTGCGTCGGGAATTGGGAAAA
ACTGGAGTCTTCATGAAGTC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MRMPFIDSEASRREVPWTTRTLRLGSLFASTLFVAACAVGPDYHKPPQDIPATFKEGTDWQRAQVNPQGAISSTWWLDYH
DDTLSSLVNQALRANQSIASAEAAYRLARATIDANVASLFPTVGVDASGSRSRTAAVESVTSQPYMSNLASVSASASWEV
DLWGSVRREIESAKASAQASDAQLAGERLSIAASVAVDYFSLRQADVDIASLKQQQGIDARILEMDRAGFVQGTVSNDEV
LEAQDTLELVIAELQATETSREQDEHAIAVLTGTPPASFALPPQPGYVFTTPVVPPGFPSQLLERRYDVVSAERTAAAAN
ARIGVAKAAFFPTLTLSAQGGFEHNALANLFSVPNRFWTLGPDLAATIFDGGARSAAVHEAEATYDEQIATYRGTVLSAF
QSVEDSLSSYNHLCKQEQAYANIYERNQRLFSSQQAQMRAGTTSEQALLTQQITLLRAGQSLRDTQAALTESSVTLVKNL
GGGWQWDDARGAAVSISANAAQQSSDQKSGERSQ

Sequences:

>Translated_514_residues
MRMPFIDSEASRREVPWTTRTLRLGSLFASTLFVAACAVGPDYHKPPQDIPATFKEGTDWQRAQVNPQGAISSTWWLDYH
DDTLSSLVNQALRANQSIASAEAAYRLARATIDANVASLFPTVGVDASGSRSRTAAVESVTSQPYMSNLASVSASASWEV
DLWGSVRREIESAKASAQASDAQLAGERLSIAASVAVDYFSLRQADVDIASLKQQQGIDARILEMDRAGFVQGTVSNDEV
LEAQDTLELVIAELQATETSREQDEHAIAVLTGTPPASFALPPQPGYVFTTPVVPPGFPSQLLERRYDVVSAERTAAAAN
ARIGVAKAAFFPTLTLSAQGGFEHNALANLFSVPNRFWTLGPDLAATIFDGGARSAAVHEAEATYDEQIATYRGTVLSAF
QSVEDSLSSYNHLCKQEQAYANIYERNQRLFSSQQAQMRAGTTSEQALLTQQITLLRAGQSLRDTQAALTESSVTLVKNL
GGGWQWDDARGAAVSISANAAQQSSDQKSGERSQ
>Mature_514_residues
MRMPFIDSEASRREVPWTTRTLRLGSLFASTLFVAACAVGPDYHKPPQDIPATFKEGTDWQRAQVNPQGAISSTWWLDYH
DDTLSSLVNQALRANQSIASAEAAYRLARATIDANVASLFPTVGVDASGSRSRTAAVESVTSQPYMSNLASVSASASWEV
DLWGSVRREIESAKASAQASDAQLAGERLSIAASVAVDYFSLRQADVDIASLKQQQGIDARILEMDRAGFVQGTVSNDEV
LEAQDTLELVIAELQATETSREQDEHAIAVLTGTPPASFALPPQPGYVFTTPVVPPGFPSQLLERRYDVVSAERTAAAAN
ARIGVAKAAFFPTLTLSAQGGFEHNALANLFSVPNRFWTLGPDLAATIFDGGARSAAVHEAEATYDEQIATYRGTVLSAF
QSVEDSLSSYNHLCKQEQAYANIYERNQRLFSSQQAQMRAGTTSEQALLTQQITLLRAGQSLRDTQAALTESSVTLVKNL
GGGWQWDDARGAAVSISANAAQQSSDQKSGERSQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=25.5364806866953, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI2367348, Length=483, Percent_Identity=24.0165631469979, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55324; Mature: 55324

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMPFIDSEASRREVPWTTRTLRLGSLFASTLFVAACAVGPDYHKPPQDIPATFKEGTDW
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCC
QRAQVNPQGAISSTWWLDYHDDTLSSLVNQALRANQSIASAEAAYRLARATIDANVASLF
CCEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHC
PTVGVDASGSRSRTAAVESVTSQPYMSNLASVSASASWEVDLWGSVRREIESAKASAQAS
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCC
DAQLAGERLSIAASVAVDYFSLRQADVDIASLKQQQGIDARILEMDRAGFVQGTVSNDEV
HHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEECCCCCHH
LEAQDTLELVIAELQATETSREQDEHAIAVLTGTPPASFALPPQPGYVFTTPVVPPGFPS
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCHH
QLLERRYDVVSAERTAAAANARIGVAKAAFFPTLTLSAQGGFEHNALANLFSVPNRFWTL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCEEC
GPDLAATIFDGGARSAAVHEAEATYDEQIATYRGTVLSAFQSVEDSLSSYNHLCKQEQAY
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANIYERNQRLFSSQQAQMRAGTTSEQALLTQQITLLRAGQSLRDTQAALTESSVTLVKNL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GGGWQWDDARGAAVSISANAAQQSSDQKSGERSQ
CCCCCCCCCCCCEEEEECCHHHHCCHHCCCCCCC
>Mature Secondary Structure
MRMPFIDSEASRREVPWTTRTLRLGSLFASTLFVAACAVGPDYHKPPQDIPATFKEGTDW
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCC
QRAQVNPQGAISSTWWLDYHDDTLSSLVNQALRANQSIASAEAAYRLARATIDANVASLF
CCEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHC
PTVGVDASGSRSRTAAVESVTSQPYMSNLASVSASASWEVDLWGSVRREIESAKASAQAS
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCC
DAQLAGERLSIAASVAVDYFSLRQADVDIASLKQQQGIDARILEMDRAGFVQGTVSNDEV
HHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEECCCCCHH
LEAQDTLELVIAELQATETSREQDEHAIAVLTGTPPASFALPPQPGYVFTTPVVPPGFPS
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCHH
QLLERRYDVVSAERTAAAANARIGVAKAAFFPTLTLSAQGGFEHNALANLFSVPNRFWTL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCEEC
GPDLAATIFDGGARSAAVHEAEATYDEQIATYRGTVLSAFQSVEDSLSSYNHLCKQEQAY
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANIYERNQRLFSSQQAQMRAGTTSEQALLTQQITLLRAGQSLRDTQAALTESSVTLVKNL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GGGWQWDDARGAAVSISANAAQQSSDQKSGERSQ
CCCCCCCCCCCCEEEEECCHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]