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The map label for this gene is mdtE [H]

Identifier: 91784061

GI number: 91784061

Start: 2985360

End: 2986571

Strand: Reverse

Name: mdtE [H]

Synonym: Bxe_A1742

Alternate gene names: 91784061

Gene position: 2986571-2985360 (Counterclockwise)

Preceding gene: 91784062

Following gene: 91784060

Centisome position: 61.0

GC content: 62.29

Gene sequence:

>1212_bases
ATGAAGTCACTACCGTTGCTGTGCGTTGTCCTTTTCATACTGTCAGCGCTTTCCGGCTGCGCAAAATCCAGTCACAGCTC
GGCAGCTCCGCGGCCGCAGGTCGGCGTGATCACCGTCCAGCCGCAGAGTGTTGCCCTGACGCGCCATCTGGTCGGACGTC
TTGCGCCATATCTCAGCGCCAACGTCACGGCGCGCGTGTCCGGTGTCCTGACCAAACGGGTCTATAAGGAAGGTACCGAC
GTCCAGGCCGGACAGGTGCTCTTCGAGATCGATCCTGCGTACTATCAGGCGCAGCTCAACAACGACCTCGGCATCCTCGG
CGAAGATCGGGCTACCTGGATGAACGACAAGGTCAACGCGGCGCGTTATCACCAGCTGCTGCCGGTCGGCTCCGTGTCGC
AACAGACAGTTGACAACGCCGATGCGACAGTGCGCAGTGACGCGGCTAAAGTCAAGGCCGATGAGGCGATGGTGGAAAGC
GCGAAGGTCAACCTCGGCTATACCAAGGTGACCTCGCCGATCCGCGGCATTGCCGGTCAGCAGCTAGTGACCGTCGGCGC
CCTCGTGGGTAGCGGTACCTCCGATGCTGGCTCCGGCGGCACCTTGCTTACCACCGTGAACGACATTGACCAGCTCTACG
TGAACTTCACCTTGAGCGCCGCGGATCTGCTGATGCTGCGAGAAGCAGGTGCCCGCGGCGACGTCGCGCTCGCCGAGCCA
GACAGGACGACCGTGCAGATCACGCTGCCAGACGGCAGCGCGTACGCCCAACTGGCCACCCTCGACTTCTCCGACGTCTC
CGTGAATGCCACTACGGGTGCGGTCAATCTGCGTGCACTGGTACCGAACCCTCAGCGCACGATACTCCCGGGCATGTATG
TGACGTTGAACGTGACCTTGGGCCAACAGCGCAACGTGTTCCTGGTGCCACAGGAGTCGCTTCAGCGTGACACCGTAGGC
GCTTATGTATTCGTTGCCGATGCGAGCGGCAAGGTGGCGCGCGAGGATGTGGCCGCCAACGACAGCTTGGGCACCAACTG
GATCGTTACCACAGGCCTTACGGCCGGCGATCAGGTGATTGTCTCCGGTCTGCAAGGCCTGCATGAGGGCGATCCTGTCA
AGTCTCATCCATGGCATGCGCCGTCGGCGGCCGCGCCGCAAGACGGATCAGCATTGGCTGCAGGCAGCCGGACTGCCGTC
AACGCACACTAA

Upstream 100 bases:

>100_bases
CGCAATGAAAGATCGCCTTGAACTTAGCCGCTTTGGGGGGCGAAGCATCGAGTCCAGCGATAGCCGGTTGCGTCGGGAAT
TGGGAAAAACTGGAGTCTTC

Downstream 100 bases:

>100_bases
GGAAGCTGCCCCATGCCTCGATTTTTTATCGATCGCCCCGTCTTTGCATGGGTGATCCCGATTCTGATCTGCATCATCGG
TGTCATTTCGATGATGCATC

Product: RND efflux system membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKSLPLLCVVLFILSALSGCAKSSHSSAAPRPQVGVITVQPQSVALTRHLVGRLAPYLSANVTARVSGVLTKRVYKEGTD
VQAGQVLFEIDPAYYQAQLNNDLGILGEDRATWMNDKVNAARYHQLLPVGSVSQQTVDNADATVRSDAAKVKADEAMVES
AKVNLGYTKVTSPIRGIAGQQLVTVGALVGSGTSDAGSGGTLLTTVNDIDQLYVNFTLSAADLLMLREAGARGDVALAEP
DRTTVQITLPDGSAYAQLATLDFSDVSVNATTGAVNLRALVPNPQRTILPGMYVTLNVTLGQQRNVFLVPQESLQRDTVG
AYVFVADASGKVAREDVAANDSLGTNWIVTTGLTAGDQVIVSGLQGLHEGDPVKSHPWHAPSAAAPQDGSALAAGSRTAV
NAH

Sequences:

>Translated_403_residues
MKSLPLLCVVLFILSALSGCAKSSHSSAAPRPQVGVITVQPQSVALTRHLVGRLAPYLSANVTARVSGVLTKRVYKEGTD
VQAGQVLFEIDPAYYQAQLNNDLGILGEDRATWMNDKVNAARYHQLLPVGSVSQQTVDNADATVRSDAAKVKADEAMVES
AKVNLGYTKVTSPIRGIAGQQLVTVGALVGSGTSDAGSGGTLLTTVNDIDQLYVNFTLSAADLLMLREAGARGDVALAEP
DRTTVQITLPDGSAYAQLATLDFSDVSVNATTGAVNLRALVPNPQRTILPGMYVTLNVTLGQQRNVFLVPQESLQRDTVG
AYVFVADASGKVAREDVAANDSLGTNWIVTTGLTAGDQVIVSGLQGLHEGDPVKSHPWHAPSAAAPQDGSALAAGSRTAV
NAH
>Mature_403_residues
MKSLPLLCVVLFILSALSGCAKSSHSSAAPRPQVGVITVQPQSVALTRHLVGRLAPYLSANVTARVSGVLTKRVYKEGTD
VQAGQVLFEIDPAYYQAQLNNDLGILGEDRATWMNDKVNAARYHQLLPVGSVSQQTVDNADATVRSDAAKVKADEAMVES
AKVNLGYTKVTSPIRGIAGQQLVTVGALVGSGTSDAGSGGTLLTTVNDIDQLYVNFTLSAADLLMLREAGARGDVALAEP
DRTTVQITLPDGSAYAQLATLDFSDVSVNATTGAVNLRALVPNPQRTILPGMYVTLNVTLGQQRNVFLVPQESLQRDTVG
AYVFVADASGKVAREDVAANDSLGTNWIVTTGLTAGDQVIVSGLQGLHEGDPVKSHPWHAPSAAAPQDGSALAAGSRTAV
NAH

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=367, Percent_Identity=41.1444141689373, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1786668, Length=392, Percent_Identity=39.7959183673469, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1789665, Length=373, Percent_Identity=38.8739946380697, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI87082036, Length=348, Percent_Identity=31.3218390804598, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081796, Length=349, Percent_Identity=24.9283667621776, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42091; Mature: 42091

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLPLLCVVLFILSALSGCAKSSHSSAAPRPQVGVITVQPQSVALTRHLVGRLAPYLSA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
NVTARVSGVLTKRVYKEGTDVQAGQVLFEIDPAYYQAQLNNDLGILGEDRATWMNDKVNA
CCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCEEECCCCCCCCCCCCH
ARYHQLLPVGSVSQQTVDNADATVRSDAAKVKADEAMVESAKVNLGYTKVTSPIRGIAGQ
HHHHHCCCCCCCCHHHCCCCCCEECCCHHHHHHHHHHHHHHEEECCHHHHCCHHHHCCCC
QLVTVGALVGSGTSDAGSGGTLLTTVNDIDQLYVNFTLSAADLLMLREAGARGDVALAEP
CEEEEEHHCCCCCCCCCCCCEEEEEHHCCEEEEEEEEECHHHHHHHHHCCCCCCEEEECC
DRTTVQITLPDGSAYAQLATLDFSDVSVNATTGAVNLRALVPNPQRTILPGMYVTLNVTL
CCEEEEEEECCCCCEEEEEEEECCCEEEEEECCEEEEEEECCCCCCEECCCEEEEEEEEE
GQQRNVFLVPQESLQRDTVGAYVFVADASGKVAREDVAANDSLGTNWIVTTGLTAGDQVI
CCCCCEEEECHHHCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEEEECCCCCHHHH
VSGLQGLHEGDPVKSHPWHAPSAAAPQDGSALAAGSRTAVNAH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEECCC
>Mature Secondary Structure
MKSLPLLCVVLFILSALSGCAKSSHSSAAPRPQVGVITVQPQSVALTRHLVGRLAPYLSA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
NVTARVSGVLTKRVYKEGTDVQAGQVLFEIDPAYYQAQLNNDLGILGEDRATWMNDKVNA
CCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCEEECCCCCCCCCCCCH
ARYHQLLPVGSVSQQTVDNADATVRSDAAKVKADEAMVESAKVNLGYTKVTSPIRGIAGQ
HHHHHCCCCCCCCHHHCCCCCCEECCCHHHHHHHHHHHHHHEEECCHHHHCCHHHHCCCC
QLVTVGALVGSGTSDAGSGGTLLTTVNDIDQLYVNFTLSAADLLMLREAGARGDVALAEP
CEEEEEHHCCCCCCCCCCCCEEEEEHHCCEEEEEEEEECHHHHHHHHHCCCCCCEEEECC
DRTTVQITLPDGSAYAQLATLDFSDVSVNATTGAVNLRALVPNPQRTILPGMYVTLNVTL
CCEEEEEEECCCCCEEEEEEEECCCEEEEEECCEEEEEEECCCCCCEECCCEEEEEEEEE
GQQRNVFLVPQESLQRDTVGAYVFVADASGKVAREDVAANDSLGTNWIVTTGLTAGDQVI
CCCCCEEEECHHHCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEEEECCCCCHHHH
VSGLQGLHEGDPVKSHPWHAPSAAAPQDGSALAAGSRTAVNAH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]