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The map label for this gene is kgtP [H]

Identifier: 91783825

GI number: 91783825

Start: 2731606

End: 2732910

Strand: Reverse

Name: kgtP [H]

Synonym: Bxe_A1987

Alternate gene names: 91783825

Gene position: 2732910-2731606 (Counterclockwise)

Preceding gene: 91783827

Following gene: 91783822

Centisome position: 55.82

GC content: 63.45

Gene sequence:

>1305_bases
ATGACAAATCTCAATATTCCAATGGCAGTTTCCACGAACGACACCCGGCGCCGCATCTACGCGATTGTCGGCGCTTCATC
GGGCAATCTCGTCGAGTGGTTCGACTTTTACGTGTATTCGTTCTGCGCGCTGTACTTCGCGCCGGCGTTCTTCCCGAGCG
GCAACCCGACCACGCAGCTGCTCAACACCGCGGGCGTGTTTGCCGCCGGCTTCCTGATGCGCCCGATCGGCGGCTGGTTC
TTCGGCCGCCTTGCCGACAAGCACGGGCGGCGCACCGCGATGATGGTGTCGGTGTTCATGATGTGCGGCGGCTCGCTGGT
GATCGCGGTGCTTCCTACTTACGCGCAGATCGGCGCGCTGGCGCCGGCGCTGCTGCTGCTCGCGCGGCTGTTCCAGGGGC
TGTCGGTGGGCGGTGAATACGGCACCAGTGCGACCTATATGAGTGAAGTCGCGCTCAAGGGCCGGCGCGGTTTCTTCGCG
TCGTTCCAGTACGTCACGCTGATCGGCGGGCAGTTGTTCGCGCTGCTGGTACTGGTGATCCTGCAACAATTGTTGACCAC
TGAAGAACTGAAGGCATGGGGTTGGCGCGTGCCGTTCGTGATCGGCGCGCTCTCCGCAGTGGTTGCGCTGTATCTGCGGC
GCTCGCTGGAGGAAACCACCACGGCCGAAACACGCCATCGCAAGGAAGCCGGCACGCTGCGCGGCCTGTGGCTGCACAAG
GGCGCGTTCATGACGGTGCTCGGCTTCACGGCCGGGGGTTCGCTGATTTTCTACACGTTCACCACCTACATGCAGAAGTA
CCTGGTCAACACGGCGGGCATGCACGCGAAGACGGCGAGCAACGTGATGACCGCGGCGCTGTTCGTCTACATGCTGATGC
AGCCGGCATTCGGTGCGTTGTCGGACCGCATCGGCCGGCGCCGCTCGATGCTGTTCTTCGGGTTCTTCGCAACCATCGGC
ACGGTGCCGTTGCTGCACGCGCTGAAAGACGTGACGAGCCCGTATGCGGCGTTCGGCCTGGTGGTGGCGGCGCTGGCGAT
CGTCAGTTTCTACACGTCGATCAGCGGCCTGATCAAGGCGGAGATGTTCCCGCCGGAAGTGCGCGCGCTCGGCGTGGGGT
TGTCGTATGCGGTGGCCAATGCGATTTTCGGCGGCTCGGCGGAATATGTCGCGCTGTGGCTGAAGCAGGCGGGCAGCGAG
TCCACGTTCTACTGGTACGTGACGGCCATGTGCGCAATCGCCGGCGTCGTAGCGTTCCTCATGCGTGACCCGTCGAAAGA
AGGGTATCTGCGGCACGAACCGTGA

Upstream 100 bases:

>100_bases
TGAAACATTCATTTAGAATGCCGAACCAAGAGACGAATACAGGCGTGGAGGAACCTCGCCATTAATAATTAACAGGGGAC
ATCCAGGAGCACAAAAACCA

Downstream 100 bases:

>100_bases
GCCGCGCGCAAAGCCCGGACACCACGCGCTGATCGCGCGGGCCTGGCCGTCACGAGTCAGGTGCCTGCGCGGTCAGCGTC
GCTGTGCATGTTGTTGTCCA

Product: alpha-ketoglutarate/proton symporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTNLNIPMAVSTNDTRRRIYAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQLLNTAGVFAAGFLMRPIGGWF
FGRLADKHGRRTAMMVSVFMMCGGSLVIAVLPTYAQIGALAPALLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLFALLVLVILQQLLTTEELKAWGWRVPFVIGALSAVVALYLRRSLEETTTAETRHRKEAGTLRGLWLHK
GAFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPAFGALSDRIGRRRSMLFFGFFATIG
TVPLLHALKDVTSPYAAFGLVVAALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSE
STFYWYVTAMCAIAGVVAFLMRDPSKEGYLRHEP

Sequences:

>Translated_434_residues
MTNLNIPMAVSTNDTRRRIYAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQLLNTAGVFAAGFLMRPIGGWF
FGRLADKHGRRTAMMVSVFMMCGGSLVIAVLPTYAQIGALAPALLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLFALLVLVILQQLLTTEELKAWGWRVPFVIGALSAVVALYLRRSLEETTTAETRHRKEAGTLRGLWLHK
GAFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPAFGALSDRIGRRRSMLFFGFFATIG
TVPLLHALKDVTSPYAAFGLVVAALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSE
STFYWYVTAMCAIAGVVAFLMRDPSKEGYLRHEP
>Mature_433_residues
TNLNIPMAVSTNDTRRRIYAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQLLNTAGVFAAGFLMRPIGGWFF
GRLADKHGRRTAMMVSVFMMCGGSLVIAVLPTYAQIGALAPALLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFAS
FQYVTLIGGQLFALLVLVILQQLLTTEELKAWGWRVPFVIGALSAVVALYLRRSLEETTTAETRHRKEAGTLRGLWLHKG
AFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPAFGALSDRIGRRRSMLFFGFFATIGT
VPLLHALKDVTSPYAAFGLVVAALAIVSFYTSISGLIKAEMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSES
TFYWYVTAMCAIAGVVAFLMRDPSKEGYLRHEP

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=417, Percent_Identity=73.1414868105516, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=29.6983758700696, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=29.81220657277, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789941, Length=442, Percent_Identity=31.6742081447964, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI87082231, Length=234, Percent_Identity=30.7692307692308, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082404, Length=375, Percent_Identity=25.0666666666667, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47314; Mature: 47183

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLNIPMAVSTNDTRRRIYAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQL
CCCCCCCEEECCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LNTAGVFAAGFLMRPIGGWFFGRLADKHGRRTAMMVSVFMMCGGSLVIAVLPTYAQIGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHEEHHHHHHHHHHHH
APALLLLARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQYVTLIGGQLFALLVLVI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQQLLTTEELKAWGWRVPFVIGALSAVVALYLRRSLEETTTAETRHRKEAGTLRGLWLHK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
GAFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTAALFVYMLMQPAFGAL
CHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SDRIGRRRSMLFFGFFATIGTVPLLHALKDVTSPYAAFGLVVAALAIVSFYTSISGLIKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKQAGSESTFYWYVTAMCAIAGVVAFL
CCCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHH
MRDPSKEGYLRHEP
HCCCCCCCCCCCCC
>Mature Secondary Structure 
TNLNIPMAVSTNDTRRRIYAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNPTTQL
CCCCCCEEECCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH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HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]