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The map label for this gene is yafC [C]

Identifier: 91783822

GI number: 91783822

Start: 2729274

End: 2730158

Strand: Reverse

Name: yafC [C]

Synonym: Bxe_A1990

Alternate gene names: 91783822

Gene position: 2730158-2729274 (Counterclockwise)

Preceding gene: 91783825

Following gene: 91783821

Centisome position: 55.76

GC content: 65.99

Gene sequence:

>885_bases
GTGGATAAATTCGTCAGCATGGAAATCTTCGTCGCCGTGGTGGAGGCGGGCAGCCTGACGGCGGCCGCCGAGCGCTTCGA
TATTTCGTCGGCGATGGTGGGCAAGCATATCCGTTCGCTGGAAACCCGCCTCGTCACGCGGCTGCTGACGCGCACGACGC
GCCGGCAAAGCCTGACCGAGATTGGCCGGCAGTATTACGAGCAATGCCGGCGCATCCTCGCCGACGTGAAAGACGCCGAG
TCGCTCGCCGAGGCAATGGCCGCCGCACCGCGCGGCGTGCTCAAGGTGACGGCGCCGCTCACTTACGGCGTCGAAGTCTT
CGCTCCGGCGATGACCGAGTACCTTACCGCGTGGCCGGACATCACGCTCGAACTCGACCTTTCGAACCGCGTGATCGACC
TCGTGGAAGAGGGCTTCGACGCGTCGGTGCGTATCGGCCAGTTGCCGGATTCGAGTTTCGTCGCGCGGCCGCTGAAGCCT
TACCGGATGCGCGCATGCGCGTCGCCGGCCTATCTGGCGCGCGCCGGCACGCCGCGCACGCCGGAAGATCTGATGCAGCA
CGAATGCCTGGGGTTTCTGCACTGGGGCCGCGAAGGTTTGTGGCGCCTGGGCGGCGAGACCGCGGACGAAAACCATCTGC
GCGCCGGACGGTTTCGCGCGAACAACGGCCAGGCGCTCAAGGTGGCCGCGCTGCACGGCTTCGGTCTGGTGTTGCAGCCG
GAGGCGCTGCTCGCCAGAGAAATCGCCAGCGGCGAATTGGTGTCGGTGCTGGAAGACTATCTGCCCGAGGGCGCGCCGGT
CCATCTGCTGTATCCGCGTGATCGCCGCGCCACGCCCAAGCTGACCAGCTTCATCGATTTCGTGATCGAACGGTTCGGCG
CGTAA

Upstream 100 bases:

>100_bases
GCTGCCCAGTTTGCCGCCGCCGCTATTAGTATCACGATATCCCTACAATGCGGCTGTTGTGCGACAAACGCTTACAACCG
AAACGAACAGGAAATAGCGG

Downstream 100 bases:

>100_bases
GCCTGGCGGCCGCCCCGAGCTTCCTTGATATGCAGGAATGCCTGCCATGCTGTGGCGGCATGCATGGCGATGCATGGGCG
GTGCATGCCGACCGGTCCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDKFVSMEIFVAVVEAGSLTAAAERFDISSAMVGKHIRSLETRLVTRLLTRTTRRQSLTEIGRQYYEQCRRILADVKDAE
SLAEAMAAAPRGVLKVTAPLTYGVEVFAPAMTEYLTAWPDITLELDLSNRVIDLVEEGFDASVRIGQLPDSSFVARPLKP
YRMRACASPAYLARAGTPRTPEDLMQHECLGFLHWGREGLWRLGGETADENHLRAGRFRANNGQALKVAALHGFGLVLQP
EALLAREIASGELVSVLEDYLPEGAPVHLLYPRDRRATPKLTSFIDFVIERFGA

Sequences:

>Translated_294_residues
MDKFVSMEIFVAVVEAGSLTAAAERFDISSAMVGKHIRSLETRLVTRLLTRTTRRQSLTEIGRQYYEQCRRILADVKDAE
SLAEAMAAAPRGVLKVTAPLTYGVEVFAPAMTEYLTAWPDITLELDLSNRVIDLVEEGFDASVRIGQLPDSSFVARPLKP
YRMRACASPAYLARAGTPRTPEDLMQHECLGFLHWGREGLWRLGGETADENHLRAGRFRANNGQALKVAALHGFGLVLQP
EALLAREIASGELVSVLEDYLPEGAPVHLLYPRDRRATPKLTSFIDFVIERFGA
>Mature_294_residues
MDKFVSMEIFVAVVEAGSLTAAAERFDISSAMVGKHIRSLETRLVTRLLTRTTRRQSLTEIGRQYYEQCRRILADVKDAE
SLAEAMAAAPRGVLKVTAPLTYGVEVFAPAMTEYLTAWPDITLELDLSNRVIDLVEEGFDASVRIGQLPDSSFVARPLKP
YRMRACASPAYLARAGTPRTPEDLMQHECLGFLHWGREGLWRLGGETADENHLRAGRFRANNGQALKVAALHGFGLVLQP
EALLAREIASGELVSVLEDYLPEGAPVHLLYPRDRRATPKLTSFIDFVIERFGA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=35.4385964912281, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=32.1917808219178, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=31.1643835616438, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=29.5138888888889, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=31.4878892733564, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787128, Length=281, Percent_Identity=29.5373665480427, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787589, Length=283, Percent_Identity=31.095406360424, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=24.3986254295533, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=24.0418118466899, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=23.0769230769231, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787879, Length=150, Percent_Identity=31.3333333333333, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32615; Mature: 32615

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFVSMEIFVAVVEAGSLTAAAERFDISSAMVGKHIRSLETRLVTRLLTRTTRRQSLTE
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRQYYEQCRRILADVKDAESLAEAMAAAPRGVLKVTAPLTYGVEVFAPAMTEYLTAWPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
ITLELDLSNRVIDLVEEGFDASVRIGQLPDSSFVARPLKPYRMRACASPAYLARAGTPRT
EEEEEECCHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCHHHHHHHCCCHHHHCCCCCCC
PEDLMQHECLGFLHWGREGLWRLGGETADENHLRAGRFRANNGQALKVAALHGFGLVLQP
HHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEEECCCEEECC
EALLAREIASGELVSVLEDYLPEGAPVHLLYPRDRRATPKLTSFIDFVIERFGA
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKFVSMEIFVAVVEAGSLTAAAERFDISSAMVGKHIRSLETRLVTRLLTRTTRRQSLTE
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRQYYEQCRRILADVKDAESLAEAMAAAPRGVLKVTAPLTYGVEVFAPAMTEYLTAWPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
ITLELDLSNRVIDLVEEGFDASVRIGQLPDSSFVARPLKPYRMRACASPAYLARAGTPRT
EEEEEECCHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCHHHHHHHCCCHHHHCCCCCCC
PEDLMQHECLGFLHWGREGLWRLGGETADENHLRAGRFRANNGQALKVAALHGFGLVLQP
HHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEEECCCEEECC
EALLAREIASGELVSVLEDYLPEGAPVHLLYPRDRRATPKLTSFIDFVIERFGA
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]