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The map label for this gene is hsrA [H]

Identifier: 91783791

GI number: 91783791

Start: 2693340

End: 2694740

Strand: Direct

Name: hsrA [H]

Synonym: Bxe_A2021

Alternate gene names: 91783791

Gene position: 2693340-2694740 (Clockwise)

Preceding gene: 91783787

Following gene: 91783793

Centisome position: 55.01

GC content: 65.6

Gene sequence:

>1401_bases
ATGACCAAGGATTCTTCCCAGACCGCTCTGCTCTGGATCGTCGCTGCCGGTTTTTTCATGCAGTCCCTCGACACGACGAT
CGTCAATACCGCCTTGCCGTCGATTGCGAACAGCCTGCACGTCGCACCGCTTGCCATGCAGCCCATCGTGGTCGCCTACA
CCCTGACTATGGCGATGCTCACGCCCGCCTCCGGCTGGCTCGCCGACCGCTTCGGCACGCGGCGCGTCTATTTCGCGGCC
ATTCTGCTGTTCGTGATCGGCTCGATCTGCTGCGCGAGCGCGCATACGCTCGGCCAACTGGTGATGGCGCGCGTGCTGCA
AGGCGTGGGCGGCTCGATGCTGCTGCCGATCGGACGGCTCGCCGTGTTGCGCAGCGTGACGGGCGAACAGTACGTCGCGG
CCCTCGCATTCATTTCGGTCGCGGGACAGTTGGGACCGATCGCCGGGCCGACGCTCGGCGGCTGGTTCGTTCAGGCGATC
ACGTGGCACTGGATTTTCCTCATCAACGTGCCGATCGGCGCCGTGGGTCTGTACGCGGTGCAGCGCTTCCTGCCCTCGCA
CGGCGAAACCCAGGCGCCGCCGTTCGACTTCGTCGGCTGCGGGCTGCTTTCGCTGTGCATGATCGCGTTCTCGCTCGCCA
TCGACGCGCCCATGCCCACGCATCGGCTGGCATGGTCCGCGGCGCTCTTCGTACTCGCCGCGCTGAGCGCGCTCGCCTAC
ATTCCGCATGCGAAACGCCGGGTCAATCCGCTCTTCAAGCTTTCGCTGTTTCGCGAGCCCAATTTCAGCGTCGGTCTGAT
CGGCAACCTGGTGTGCCGCATCGGTTCGAGCGCCGTGCCGTTTCTCGTGCCCTTGCTGCTGCAACTGCAACTGGGCTATT
CGCCGCTGCATTCCGGCCTGATGATGCTGCCCGCCGCGCTGGCCGGCACCGTCGCCAAACGCTGGATCGCGCCGCTCGTG
CGCCGCTATGGCTACGACACGTTCCTGCTCGTCAACACCGTGATCGTCGGTTCCTCGATCGTGGCCTTTGCGCTGATTAC
GCGCGGCACGCCGCTCGTGGTCGAGATCGCGATCCTCGCCGTGTTCGGCGCCGCCAATTCCATGCAATTCGCGGCGATGA
ACAGCGTCACGCTCAAGGACCTCTCGCATGCCGACGCCGGCAGCGGCAACAGCCTCTTTTCGATGGTGCAGATGCTGGCG
ATCGGGCTGGGTGTATCGATCGGCGGCGGGCTGGTGAATCTCTTCTCCGCGCAGGTGGGATCGGCGGCACTCGGGTTTCG
CCTCGCGTTCGTGTGCGTGGGCGTGATCACGCTGGTGTCGGCCTGGGTGTTTCGTCATCTCGACGAAGCGCCTGCCTCGC
GCACGGTGCGCGGGCAAGCGACTCAAGGCGCGGGGCGCTGA

Upstream 100 bases:

>100_bases
GGTTCCCGATTGACGAGGATGTCAATATCATGACGCAATTCCCCCAATCAATGCTCCGAGCCCAGGCGCGCCTCTGCCGG
CCCGCCGCCCCCCGTAAAAG

Downstream 100 bases:

>100_bases
GCGCCGCCGCCCTCTGCATCAGTCCGGCAAACGGATGCCGGAAAAGCCCGCGCACGCTTCGAGCAGACGCTCCTGAACAG
CCACGTTGTGCACCGCCGGA

Product: major facilitator superfamily multidrug efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTKDSSQTALLWIVAAGFFMQSLDTTIVNTALPSIANSLHVAPLAMQPIVVAYTLTMAMLTPASGWLADRFGTRRVYFAA
ILLFVIGSICCASAHTLGQLVMARVLQGVGGSMLLPIGRLAVLRSVTGEQYVAALAFISVAGQLGPIAGPTLGGWFVQAI
TWHWIFLINVPIGAVGLYAVQRFLPSHGETQAPPFDFVGCGLLSLCMIAFSLAIDAPMPTHRLAWSAALFVLAALSALAY
IPHAKRRVNPLFKLSLFREPNFSVGLIGNLVCRIGSSAVPFLVPLLLQLQLGYSPLHSGLMMLPAALAGTVAKRWIAPLV
RRYGYDTFLLVNTVIVGSSIVAFALITRGTPLVVEIAILAVFGAANSMQFAAMNSVTLKDLSHADAGSGNSLFSMVQMLA
IGLGVSIGGGLVNLFSAQVGSAALGFRLAFVCVGVITLVSAWVFRHLDEAPASRTVRGQATQGAGR

Sequences:

>Translated_466_residues
MTKDSSQTALLWIVAAGFFMQSLDTTIVNTALPSIANSLHVAPLAMQPIVVAYTLTMAMLTPASGWLADRFGTRRVYFAA
ILLFVIGSICCASAHTLGQLVMARVLQGVGGSMLLPIGRLAVLRSVTGEQYVAALAFISVAGQLGPIAGPTLGGWFVQAI
TWHWIFLINVPIGAVGLYAVQRFLPSHGETQAPPFDFVGCGLLSLCMIAFSLAIDAPMPTHRLAWSAALFVLAALSALAY
IPHAKRRVNPLFKLSLFREPNFSVGLIGNLVCRIGSSAVPFLVPLLLQLQLGYSPLHSGLMMLPAALAGTVAKRWIAPLV
RRYGYDTFLLVNTVIVGSSIVAFALITRGTPLVVEIAILAVFGAANSMQFAAMNSVTLKDLSHADAGSGNSLFSMVQMLA
IGLGVSIGGGLVNLFSAQVGSAALGFRLAFVCVGVITLVSAWVFRHLDEAPASRTVRGQATQGAGR
>Mature_465_residues
TKDSSQTALLWIVAAGFFMQSLDTTIVNTALPSIANSLHVAPLAMQPIVVAYTLTMAMLTPASGWLADRFGTRRVYFAAI
LLFVIGSICCASAHTLGQLVMARVLQGVGGSMLLPIGRLAVLRSVTGEQYVAALAFISVAGQLGPIAGPTLGGWFVQAIT
WHWIFLINVPIGAVGLYAVQRFLPSHGETQAPPFDFVGCGLLSLCMIAFSLAIDAPMPTHRLAWSAALFVLAALSALAYI
PHAKRRVNPLFKLSLFREPNFSVGLIGNLVCRIGSSAVPFLVPLLLQLQLGYSPLHSGLMMLPAALAGTVAKRWIAPLVR
RYGYDTFLLVNTVIVGSSIVAFALITRGTPLVVEIAILAVFGAANSMQFAAMNSVTLKDLSHADAGSGNSLFSMVQMLAI
GLGVSIGGGLVNLFSAQVGSAALGFRLAFVCVGVITLVSAWVFRHLDEAPASRTVRGQATQGAGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=466, Percent_Identity=42.0600858369099, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788392, Length=444, Percent_Identity=44.1441441441441, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1788710, Length=313, Percent_Identity=30.9904153354633, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=27.9012345679012, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081983, Length=312, Percent_Identity=29.8076923076923, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=173, Percent_Identity=31.2138728323699, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=156, Percent_Identity=31.4102564102564, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=145, Percent_Identity=30.3448275862069, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49121; Mature: 48990

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKDSSQTALLWIVAAGFFMQSLDTTIVNTALPSIANSLHVAPLAMQPIVVAYTLTMAML
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TPASGWLADRFGTRRVYFAAILLFVIGSICCASAHTLGQLVMARVLQGVGGSMLLPIGRL
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AVLRSVTGEQYVAALAFISVAGQLGPIAGPTLGGWFVQAITWHWIFLINVPIGAVGLYAV
HHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHH
QRFLPSHGETQAPPFDFVGCGLLSLCMIAFSLAIDAPMPTHRLAWSAALFVLAALSALAY
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IPHAKRRVNPLFKLSLFREPNFSVGLIGNLVCRIGSSAVPFLVPLLLQLQLGYSPLHSGL
CCCHHHHCCHHHEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHH
MMLPAALAGTVAKRWIAPLVRRYGYDTFLLVNTVIVGSSIVAFALITRGTPLVVEIAILA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VFGAANSMQFAAMNSVTLKDLSHADAGSGNSLFSMVQMLAIGLGVSIGGGLVNLFSAQVG
HHCCCCCCEEHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
SAALGFRLAFVCVGVITLVSAWVFRHLDEAPASRTVRGQATQGAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]