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The map label for this gene is proP [H]

Identifier: 91783736

GI number: 91783736

Start: 2633216

End: 2634592

Strand: Reverse

Name: proP [H]

Synonym: Bxe_A2078

Alternate gene names: 91783736

Gene position: 2634592-2633216 (Counterclockwise)

Preceding gene: 91783737

Following gene: 91783734

Centisome position: 53.81

GC content: 66.74

Gene sequence:

>1377_bases
ATGTCCGTAGCAGCGCAAAATGGCGCCGCCTTGCCCGCGGTCGACCAGCGGCAGGTGATGGGTGCGGTCGCGGCCTCGTG
CCTCGGTTGGGCACTGGATCTGTTCGATCTGTTCGTATTGCTGTTCGTCGCGCCGGTGGTCGGGCGGCTGTTCTTCCCGT
CCGAGCACGCCATGCTGTCGCTCGCCGCGGTCTATGCGTCGTTTGCCGTGACCTTGCTGATGCGCCCGCTCGGCTCCGCC
TGGTTCGGCTCCTATGCGGACCGCCACGGCCGCAAGGGCGCGATGATCATCGCCGTGGTCGGCGTGGGCCTTTCCACGGC
GGCGTTCGGCGTGCTGCCGACCGTCGCGCAGGTCGGGCTGGTTGCGCCGATCCTGTTTCTGGTGCTGCGGCTGGTGCAGG
GCGTGTTTGTCGGCGGAGTAGTGGCGTCCACGCATACCATCGGCACCGAATCGGTCGCGCCGAAATATCGCGGCGCGGTG
TCGGGGCTGATCGGCGGAGGCGGAGCGGGGCTCGGCGCGTTGATCGCCTCGCTCACGTATCTGGCGATGTCGGCGCTATT
CCCCGGCGCGGCGTTCGAGGTGTGGGGCTGGCGCTGCATGTTCTTCACCGGCATCATCAGCTCGGTGCTCGGGCTGTTCG
TTTTCAGCAGTCTCGAGGAATCGCCGCTGTGGAAAAAGCTCGCCGCCGAGAAGGCCGCCAAGGCCGCCGCGCAAAAGCTC
GACGCGCCGGTCGAAGTGGCGCGCTCGCCGCTGCGCACGCTGTTTTCCAGCGACTATCGCTCGATCCTTCTGGTCAACCT
GCTGCTCACCATCGGCGGCGGTAGCGGCTATTACCTGACCTCCGGCTATCTGCCGACCTTCCTCAAGGTGGTGAGCCACG
CGCCGAACGGCGCCGCGGCGGCGATTCTGCTGATCTGCAGCGTGGCGGTGGTGATCGCCTCGGTCGCGGCGGGGCATCTG
AGCACCTTCATCGGGCGCAGAAGCGCTTTCATCTGGCTCGGGGTGATTCGCCTGTTCGCGTTGCCCGCGCTCTTCCTGCT
GCTGCCCACGGCGCAGAGCATCACGATGATCGGCGTCTACGCGGTGATTCTCAGCGCGCTCGGCAGCGCGGGCTACGCGC
CGATCCTGATCTTCCTGAACGAACGGTTTCCGACCGCGATTCGCGCGACCGGCACGGGGCTTTCATGGAACATCGGCTTT
GCGATCGGCGGCATGATGCCGACTGCCGTCTCGCTGGTGGCGAAAGACGCGAGCGAATTGCCGGTGACGCTGGCCATCTT
CGTCGGTGCGATCAGCGTGATCTTCCTGACCGGCGCGTTCATCGTGCCGGAAACGCGCGGCAGACTCGATCAGGTCGCGG
CGCGCGACGCGGTCTAG

Upstream 100 bases:

>100_bases
CCGCCGACGCCAGATAACCACCAGAAGAGCACGCGAAAAGAACGTGCCTCGAAAACGGGCACGGTCGAAAGACGGCCCGC
CAATAGTCAAGGAGATGACA

Downstream 100 bases:

>100_bases
TTCGCGGGCAAGCGGCTTGCGGCGCGTTTCACCGTTTCACGCGGCGGGCGTGCCCGTCGTCACGCGCCGGATCTCTTCGC
CGATGATGTCGATCAGCGCG

Product: major facilitator superfamily metabolite/H(+) symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSVAAQNGAALPAVDQRQVMGAVAASCLGWALDLFDLFVLLFVAPVVGRLFFPSEHAMLSLAAVYASFAVTLLMRPLGSA
WFGSYADRHGRKGAMIIAVVGVGLSTAAFGVLPTVAQVGLVAPILFLVLRLVQGVFVGGVVASTHTIGTESVAPKYRGAV
SGLIGGGGAGLGALIASLTYLAMSALFPGAAFEVWGWRCMFFTGIISSVLGLFVFSSLEESPLWKKLAAEKAAKAAAQKL
DAPVEVARSPLRTLFSSDYRSILLVNLLLTIGGGSGYYLTSGYLPTFLKVVSHAPNGAAAAILLICSVAVVIASVAAGHL
STFIGRRSAFIWLGVIRLFALPALFLLLPTAQSITMIGVYAVILSALGSAGYAPILIFLNERFPTAIRATGTGLSWNIGF
AIGGMMPTAVSLVAKDASELPVTLAIFVGAISVIFLTGAFIVPETRGRLDQVAARDAV

Sequences:

>Translated_458_residues
MSVAAQNGAALPAVDQRQVMGAVAASCLGWALDLFDLFVLLFVAPVVGRLFFPSEHAMLSLAAVYASFAVTLLMRPLGSA
WFGSYADRHGRKGAMIIAVVGVGLSTAAFGVLPTVAQVGLVAPILFLVLRLVQGVFVGGVVASTHTIGTESVAPKYRGAV
SGLIGGGGAGLGALIASLTYLAMSALFPGAAFEVWGWRCMFFTGIISSVLGLFVFSSLEESPLWKKLAAEKAAKAAAQKL
DAPVEVARSPLRTLFSSDYRSILLVNLLLTIGGGSGYYLTSGYLPTFLKVVSHAPNGAAAAILLICSVAVVIASVAAGHL
STFIGRRSAFIWLGVIRLFALPALFLLLPTAQSITMIGVYAVILSALGSAGYAPILIFLNERFPTAIRATGTGLSWNIGF
AIGGMMPTAVSLVAKDASELPVTLAIFVGAISVIFLTGAFIVPETRGRLDQVAARDAV
>Mature_457_residues
SVAAQNGAALPAVDQRQVMGAVAASCLGWALDLFDLFVLLFVAPVVGRLFFPSEHAMLSLAAVYASFAVTLLMRPLGSAW
FGSYADRHGRKGAMIIAVVGVGLSTAAFGVLPTVAQVGLVAPILFLVLRLVQGVFVGGVVASTHTIGTESVAPKYRGAVS
GLIGGGGAGLGALIASLTYLAMSALFPGAAFEVWGWRCMFFTGIISSVLGLFVFSSLEESPLWKKLAAEKAAKAAAQKLD
APVEVARSPLRTLFSSDYRSILLVNLLLTIGGGSGYYLTSGYLPTFLKVVSHAPNGAAAAILLICSVAVVIASVAAGHLS
TFIGRRSAFIWLGVIRLFALPALFLLLPTAQSITMIGVYAVILSALGSAGYAPILIFLNERFPTAIRATGTGLSWNIGFA
IGGMMPTAVSLVAKDASELPVTLAIFVGAISVIFLTGAFIVPETRGRLDQVAARDAV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=442, Percent_Identity=28.5067873303167, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1789941, Length=398, Percent_Identity=28.894472361809, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788292, Length=393, Percent_Identity=27.4809160305344, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788942, Length=415, Percent_Identity=27.2289156626506, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082404, Length=401, Percent_Identity=26.4339152119701, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082231, Length=273, Percent_Identity=28.9377289377289, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47455; Mature: 47323

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SVAAQNGAALPAVDQRQVMGAVAASCLGWALDLFDLFVLLFVAPVVGRLFFPSEHAMLS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH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CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]