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The map label for this gene is hisJ [H]

Identifier: 91782905

GI number: 91782905

Start: 1701306

End: 1702100

Strand: Direct

Name: hisJ [H]

Synonym: Bxe_A2917

Alternate gene names: 91782905

Gene position: 1701306-1702100 (Clockwise)

Preceding gene: 91782904

Following gene: 91782907

Centisome position: 34.75

GC content: 62.26

Gene sequence:

>795_bases
ATGAAGATGAATTGGCGAAACATGGCCGCGCTCGCGCTGTTCGCGGCGGCCACGGCTACGGCCGGCACCGCATCGGCTGC
GGATATCAAGGAAGTGCATTTCGGCGTCGAGGCGTCGTATGCGCCGTTCGAATCGAAGTCGCCGTCGGGCGAACTGCAAG
GTTTCGACATCGACGTCGGCAATGCCGTGTGCGCGAAGCTGAAGGCCAGATGCGTGTGGGTCGAGAACTCGTTCGACGGT
CTGATCCCGGCCTTGCAGGCGCGCAAGTTCAATGCCATCAACTCGGATATGACGATCACGGACCAACGCCGCCAGGCGAT
CGATTTCACCGATCCGATCTACACGATCCCGAATCAGATGATCGCGAAGAAGGGCAGCGGTCTGCTGCCGACGCCGGCTT
CGCTCAAGGGCAAGCATGTGGGCGTGCTGCAGGGCACGATTCAGGAAACCTACGCGAAGGCGCGCTGGGCGCCGGCCGGC
GTCGATGTCGTGCCGTATCAGACGCAGGATCAGATTTACGCCGACCTCGCTTCGGGCCGTCTCGATGCGGCGTTCCAGGA
CGCGGAGGCCGCGTCGAAAGGCTTCCTGAAGAAGCCGCAAGGCGCCGGTTTCGACTTTGCCGGTCCGGCTGTCACCGACG
AAAAGCTGCTGGGCGCGGGCGTTGGCTTCGGCGTTCGCAAGGGCGACAAGGCATTGAAAGACGCGCTGAACCAGGCGCTC
AGGGAACTGAAGGCGGACGGCACGATCGACCGCTTTGCGGCCAGATACTTCGACGTGAAAGTGGTATTGAAGTGA

Upstream 100 bases:

>100_bases
TCACACCGGCCGCACGAGTTTGAATCGCAATTTTGGCGCGGCAATCATGCTTGCGCGGATTCGGCTCCGTCTCACTCAAT
ACCGTAGAGGAACACCCGTA

Downstream 100 bases:

>100_bases
CGCGCGCCTCGCGCGTTTTCGCTGTCTGTATCTGATGGTGCGAATGCAAACGGCCGCGGCCGTTTGCATTCGAGGTGTGA
CGCATTGGCACCCGCGTTGG

Product: ABC lysine/arginine/ornithine transporter, periplasmic ligand binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKMNWRNMAALALFAAATATAGTASAADIKEVHFGVEASYAPFESKSPSGELQGFDIDVGNAVCAKLKARCVWVENSFDG
LIPALQARKFNAINSDMTITDQRRQAIDFTDPIYTIPNQMIAKKGSGLLPTPASLKGKHVGVLQGTIQETYAKARWAPAG
VDVVPYQTQDQIYADLASGRLDAAFQDAEAASKGFLKKPQGAGFDFAGPAVTDEKLLGAGVGFGVRKGDKALKDALNQAL
RELKADGTIDRFAARYFDVKVVLK

Sequences:

>Translated_264_residues
MKMNWRNMAALALFAAATATAGTASAADIKEVHFGVEASYAPFESKSPSGELQGFDIDVGNAVCAKLKARCVWVENSFDG
LIPALQARKFNAINSDMTITDQRRQAIDFTDPIYTIPNQMIAKKGSGLLPTPASLKGKHVGVLQGTIQETYAKARWAPAG
VDVVPYQTQDQIYADLASGRLDAAFQDAEAASKGFLKKPQGAGFDFAGPAVTDEKLLGAGVGFGVRKGDKALKDALNQAL
RELKADGTIDRFAARYFDVKVVLK
>Mature_264_residues
MKMNWRNMAALALFAAATATAGTASAADIKEVHFGVEASYAPFESKSPSGELQGFDIDVGNAVCAKLKARCVWVENSFDG
LIPALQARKFNAINSDMTITDQRRQAIDFTDPIYTIPNQMIAKKGSGLLPTPASLKGKHVGVLQGTIQETYAKARWAPAG
VDVVPYQTQDQIYADLASGRLDAAFQDAEAASKGFLKKPQGAGFDFAGPAVTDEKLLGAGVGFGVRKGDKALKDALNQAL
RELKADGTIDRFAARYFDVKVVLK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=51.2931034482759, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=50.78125, Blast_Score=264, Evalue=4e-72,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=37.2, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=32.2448979591837, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788228, Length=225, Percent_Identity=32.8888888888889, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=28.968253968254, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28220; Mature: 28220

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNWRNMAALALFAAATATAGTASAADIKEVHFGVEASYAPFESKSPSGELQGFDIDVG
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECC
NAVCAKLKARCVWVENSFDGLIPALQARKFNAINSDMTITDQRRQAIDFTDPIYTIPNQM
HHHHHHHHEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCCCHHHCCHHH
IAKKGSGLLPTPASLKGKHVGVLQGTIQETYAKARWAPAGVDVVPYQTQDQIYADLASGR
HHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCC
LDAAFQDAEAASKGFLKKPQGAGFDFAGPAVTDEKLLGAGVGFGVRKGDKALKDALNQAL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
RELKADGTIDRFAARYFDVKVVLK
HHHHCCCCHHHHHHHHEEEEEEEC
>Mature Secondary Structure
MKMNWRNMAALALFAAATATAGTASAADIKEVHFGVEASYAPFESKSPSGELQGFDIDVG
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECC
NAVCAKLKARCVWVENSFDGLIPALQARKFNAINSDMTITDQRRQAIDFTDPIYTIPNQM
HHHHHHHHEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCCCHHHCCHHH
IAKKGSGLLPTPASLKGKHVGVLQGTIQETYAKARWAPAGVDVVPYQTQDQIYADLASGR
HHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCC
LDAAFQDAEAASKGFLKKPQGAGFDFAGPAVTDEKLLGAGVGFGVRKGDKALKDALNQAL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
RELKADGTIDRFAARYFDVKVVLK
HHHHCCCCHHHHHHHHEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]