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The map label for this gene is citH [H]

Identifier: 91782796

GI number: 91782796

Start: 1560991

End: 1562298

Strand: Direct

Name: citH [H]

Synonym: Bxe_A3029

Alternate gene names: 91782796

Gene position: 1560991-1562298 (Clockwise)

Preceding gene: 91782795

Following gene: 91782797

Centisome position: 31.88

GC content: 63.0

Gene sequence:

>1308_bases
ATGCAGGGAACGCTTTCATCCAACCTTCCACTCTCCGTGGATACGCTCGCGCGGCAGCGGCGCCGCGCCATTATCGCCAC
GGTGCTCGGCAATGGGCTCGAGTGGTTCGACTTCACCGTGTATAGTTTTTTCGCCGTCATCATTGCGAAGCTGTTTTTTC
CGACCGGCAATGAGCTGAGCTCGCTGCTGCTCGCCGTGGCCACCTTCGGCGTCGGCTTTTTCATGCGGCCGGTGGGCGGC
ATCGTGCTCGGCGTGTATGCGGACAAAGTGGGCCGGAAGGCGGCGCTCTCGCTGACCATTCTGCTGATGGCGGCGGGCAC
CGCGCTGATCGGCCTCGCGCCGACCTATGACCAGATCGGTCTGTGGGCGCCGGTGCTGATCGTGGTAGCGCGCCTGCTGC
AGGGCTTTTCAGCCGGCGGCGAGATGGGCAGCGCGACCGCCTTTCTCACGGAATACGCGCCCGTGGAGAAGCGGGCGTTC
TATTCGAGCTGGATCCAGTCGAGCATCGGCTTTGCGGTGCTGCTGGGCGCGGCGGTCGGCACGTTCGTGACGGCGAGCCT
GAGTGCCGACGCACTGCATTCGTGGGGCTGGAGAATGCCGTTCCTGATCGGCATTCTGATCGGGCCGGTCGGTTATTTCA
TTCGCAGCCGCATGGACGAAACACCGGCGTTCAGCGCCGTGGCCGAAGAAGCCCGGAACGATTCTCCGCTTGCCGAAGTA
TTCCGCCGTTTTCCGCGCGAGACGTTCGCCAGTTTTTCGATGGTGATTCTGTGGACCGTATGCACTTATGTGCTGCTGTT
CTATATGCCGACCTATTCGGTGCGCACCCTGCACCTGCCGCAGTCCACCGGCTTTCTTGCCGGTATGCTCGGCGGTCTGA
TGATCATGTGCTTCTCGCCGGTGGTCGGCAGCCTGGCCGACCGCTTCGGCCGCCGCCGTTTTCTCTCGGGTGCGGCGGTC
TTGATCCTGCTGCTCGCGTGGCCGATGTTCACGTATATCAACCGGGTGCCTGGCCTCGCTTCGCTGATGGTGTTTCAGGG
CGTGTTCGGCGTGCTGATCGCCGCCTATACCGGGCCGATCCTTGCTGCGTTCTCCGAACTGTTTCCGACCAAGGTGCTGT
CGACAGGTCTCTCCGTTGCCTATAACTTCGCGGTGACGATTTTCGGCGGCTTCGCGCCGTTCTTTATCACGTGGCTGATC
GCCTCGACCGGCAGCAACATGGCGCCCGCCATCTACGTGATGATCGCCGCGGCCGTCAGTCTGGCGGGTACGTTCTTCGT
GCGTGATCCGCGCCGGGGCGGGGTCTGA

Upstream 100 bases:

>100_bases
CTCTAGGATCGGCGTTATGCATGCGAAAGCGCCGTCCGGTCATGGCGTGCCGCAATGCCGACACTACCGCCCGACAGGCA
GCCAGATGAGGAAAGGGACC

Downstream 100 bases:

>100_bases
AGGCAAGAATGAAGCGGCGTGTCCGGCGGTATTGGCCGGAAGCATTTCCTCATCAAGTCAGGAACATATCAATGAGCATT
ACGGTAATCAGCGGCGGCAA

Product: major facilitator superfamily metabolite/H(+) symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQGTLSSNLPLSVDTLARQRRRAIIATVLGNGLEWFDFTVYSFFAVIIAKLFFPTGNELSSLLLAVATFGVGFFMRPVGG
IVLGVYADKVGRKAALSLTILLMAAGTALIGLAPTYDQIGLWAPVLIVVARLLQGFSAGGEMGSATAFLTEYAPVEKRAF
YSSWIQSSIGFAVLLGAAVGTFVTASLSADALHSWGWRMPFLIGILIGPVGYFIRSRMDETPAFSAVAEEARNDSPLAEV
FRRFPRETFASFSMVILWTVCTYVLLFYMPTYSVRTLHLPQSTGFLAGMLGGLMIMCFSPVVGSLADRFGRRRFLSGAAV
LILLLAWPMFTYINRVPGLASLMVFQGVFGVLIAAYTGPILAAFSELFPTKVLSTGLSVAYNFAVTIFGGFAPFFITWLI
ASTGSNMAPAIYVMIAAAVSLAGTFFVRDPRRGGV

Sequences:

>Translated_435_residues
MQGTLSSNLPLSVDTLARQRRRAIIATVLGNGLEWFDFTVYSFFAVIIAKLFFPTGNELSSLLLAVATFGVGFFMRPVGG
IVLGVYADKVGRKAALSLTILLMAAGTALIGLAPTYDQIGLWAPVLIVVARLLQGFSAGGEMGSATAFLTEYAPVEKRAF
YSSWIQSSIGFAVLLGAAVGTFVTASLSADALHSWGWRMPFLIGILIGPVGYFIRSRMDETPAFSAVAEEARNDSPLAEV
FRRFPRETFASFSMVILWTVCTYVLLFYMPTYSVRTLHLPQSTGFLAGMLGGLMIMCFSPVVGSLADRFGRRRFLSGAAV
LILLLAWPMFTYINRVPGLASLMVFQGVFGVLIAAYTGPILAAFSELFPTKVLSTGLSVAYNFAVTIFGGFAPFFITWLI
ASTGSNMAPAIYVMIAAAVSLAGTFFVRDPRRGGV
>Mature_435_residues
MQGTLSSNLPLSVDTLARQRRRAIIATVLGNGLEWFDFTVYSFFAVIIAKLFFPTGNELSSLLLAVATFGVGFFMRPVGG
IVLGVYADKVGRKAALSLTILLMAAGTALIGLAPTYDQIGLWAPVLIVVARLLQGFSAGGEMGSATAFLTEYAPVEKRAF
YSSWIQSSIGFAVLLGAAVGTFVTASLSADALHSWGWRMPFLIGILIGPVGYFIRSRMDETPAFSAVAEEARNDSPLAEV
FRRFPRETFASFSMVILWTVCTYVLLFYMPTYSVRTLHLPQSTGFLAGMLGGLMIMCFSPVVGSLADRFGRRRFLSGAAV
LILLLAWPMFTYINRVPGLASLMVFQGVFGVLIAAYTGPILAAFSELFPTKVLSTGLSVAYNFAVTIFGGFAPFFITWLI
ASTGSNMAPAIYVMIAAAVSLAGTFFVRDPRRGGV

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=38.1861575178998, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1788292, Length=447, Percent_Identity=32.4384787472036, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788942, Length=441, Percent_Identity=32.4263038548753, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1789941, Length=443, Percent_Identity=33.4085778781038, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87082231, Length=234, Percent_Identity=29.4871794871795, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082404, Length=383, Percent_Identity=26.6318537859008, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46826; Mature: 46826

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHEEECCCCCCC
>Mature Secondary Structure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HHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]