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The map label for this gene is gcvA [H]

Identifier: 91782795

GI number: 91782795

Start: 1559906

End: 1560823

Strand: Direct

Name: gcvA [H]

Synonym: Bxe_A3030

Alternate gene names: 91782795

Gene position: 1559906-1560823 (Clockwise)

Preceding gene: 91782794

Following gene: 91782796

Centisome position: 31.86

GC content: 63.94

Gene sequence:

>918_bases
ATGCGCATTGCCCCGTTGCCTCCGCTCCAATGCCTGGTCGCATTCGAATCGGCCGTACGGCATGCGAGTTTCACGAAAGC
CGCCGCGGAACTGCATCTGACGCAGAGCGCGATCAGCCGGCAGATCGCACAACTGGAAGACTTCCTTGGCCGCTCGCTTT
TCGTGCGTGAGCACCGCGCATTGCGTTTGACGATCGCCGGCGAAGGTTACGCGAAGCATGTGCAATGGCTGCTTGCCAGT
TGTTCGGAAGCCACGCTGGACGTCATGAAGCGCTACGGCGATATGGAATTGACGATTGCGTGTTCGTCCGGCGTGGCGGT
GCTGTGGCTGACGCCGCGGCTCGGCGCATTTCGCGCCGCACATCCTAACGTCAGGATCAGAATGATCGTGCGCGACGGGC
TTGCGTCCCTATCGCCCGCGGAATTCGACGTGGGGCTGTACTACATCCGTCAGCGCGCCGAACCGCATTTCACCGCGCGC
CGCCTCTTCGACGAAGACGTGTACCCGGTATGTTCGCCCGGATATCTCGCCGGCCGCGTGCTGACGCCCGCCGGGCTCGT
GCACGAAACGCTGCTGGTGCAGGAGGACGGCCAGCGGCAATGGATGTCGTGGTCCGAATGGTTCCGTCTGAATCATGTGC
AGATGCCGGCGTCGCCGCAGGCGGTGGTCGTGAATCACTATCCGCAACTCGTGCAGATGGCGATTCTCGGCCAGGGCGTG
GTACTCGGCTGGCGGCACATGATCGATGCATGCCTGAGCGAAGGGCTGCTGGTGCGGGCCACGCAAGCGTCGGCGAGTCA
CGGCGGCGGCTATTACGTCGTGTCGCCGAACGACCGCTCGCAGAACCAGGCGGCGCGCCTTTTCACCCGCTGGGTGTTCG
AGCAGGCAGAAGCGCAGATGGGCAGGGCGGCGAGCTGA

Upstream 100 bases:

>100_bases
CTATTCTCAACTTGCGAGGGTTCGCACCTCCCGCGCGCGGATGATCGGACGGGGGTTTCACCCGCTATGATGGTGGCACC
TTCACTCGGTTCCTCTCATC

Downstream 100 bases:

>100_bases
AACGGGCTCGCATGCGCCAGACGCATGCATGCATGCGAATCTTTCGTTTCGGAAACAAATCAGGTCTCTCTAGGATCGGC
GTTATGCATGCGAAAGCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRIAPLPPLQCLVAFESAVRHASFTKAAAELHLTQSAISRQIAQLEDFLGRSLFVREHRALRLTIAGEGYAKHVQWLLAS
CSEATLDVMKRYGDMELTIACSSGVAVLWLTPRLGAFRAAHPNVRIRMIVRDGLASLSPAEFDVGLYYIRQRAEPHFTAR
RLFDEDVYPVCSPGYLAGRVLTPAGLVHETLLVQEDGQRQWMSWSEWFRLNHVQMPASPQAVVVNHYPQLVQMAILGQGV
VLGWRHMIDACLSEGLLVRATQASASHGGGYYVVSPNDRSQNQAARLFTRWVFEQAEAQMGRAAS

Sequences:

>Translated_305_residues
MRIAPLPPLQCLVAFESAVRHASFTKAAAELHLTQSAISRQIAQLEDFLGRSLFVREHRALRLTIAGEGYAKHVQWLLAS
CSEATLDVMKRYGDMELTIACSSGVAVLWLTPRLGAFRAAHPNVRIRMIVRDGLASLSPAEFDVGLYYIRQRAEPHFTAR
RLFDEDVYPVCSPGYLAGRVLTPAGLVHETLLVQEDGQRQWMSWSEWFRLNHVQMPASPQAVVVNHYPQLVQMAILGQGV
VLGWRHMIDACLSEGLLVRATQASASHGGGYYVVSPNDRSQNQAARLFTRWVFEQAEAQMGRAAS
>Mature_305_residues
MRIAPLPPLQCLVAFESAVRHASFTKAAAELHLTQSAISRQIAQLEDFLGRSLFVREHRALRLTIAGEGYAKHVQWLLAS
CSEATLDVMKRYGDMELTIACSSGVAVLWLTPRLGAFRAAHPNVRIRMIVRDGLASLSPAEFDVGLYYIRQRAEPHFTAR
RLFDEDVYPVCSPGYLAGRVLTPAGLVHETLLVQEDGQRQWMSWSEWFRLNHVQMPASPQAVVVNHYPQLVQMAILGQGV
VLGWRHMIDACLSEGLLVRATQASASHGGGYYVVSPNDRSQNQAARLFTRWVFEQAEAQMGRAAS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=33.1103678929766, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=30.1324503311258, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786448, Length=304, Percent_Identity=30.5921052631579, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790262, Length=247, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786401, Length=306, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=26.7441860465116, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI157672245, Length=151, Percent_Identity=33.112582781457, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=112, Percent_Identity=38.3928571428571, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=284, Percent_Identity=23.943661971831, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33981; Mature: 33981

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAPLPPLQCLVAFESAVRHASFTKAAAELHLTQSAISRQIAQLEDFLGRSLFVREHRA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCE
LRLTIAGEGYAKHVQWLLASCSEATLDVMKRYGDMELTIACSSGVAVLWLTPRLGAFRAA
EEEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCCC
HPNVRIRMIVRDGLASLSPAEFDVGLYYIRQRAEPHFTARRLFDEDVYPVCSPGYLAGRV
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHCCCC
LTPAGLVHETLLVQEDGQRQWMSWSEWFRLNHVQMPASPQAVVVNHYPQLVQMAILGQGV
CCCHHHHHHHHEEECCCCHHHCCHHHHHEECCEECCCCCCEEEECCCHHHHHHHHHCCCH
VLGWRHMIDACLSEGLLVRATQASASHGGGYYVVSPNDRSQNQAARLFTRWVFEQAEAQM
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
GRAAS
CCCCC
>Mature Secondary Structure
MRIAPLPPLQCLVAFESAVRHASFTKAAAELHLTQSAISRQIAQLEDFLGRSLFVREHRA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCE
LRLTIAGEGYAKHVQWLLASCSEATLDVMKRYGDMELTIACSSGVAVLWLTPRLGAFRAA
EEEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCCC
HPNVRIRMIVRDGLASLSPAEFDVGLYYIRQRAEPHFTARRLFDEDVYPVCSPGYLAGRV
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHCCCC
LTPAGLVHETLLVQEDGQRQWMSWSEWFRLNHVQMPASPQAVVVNHYPQLVQMAILGQGV
CCCHHHHHHHHEEECCCCHHHCCHHHHHEECCEECCCCCCEEEECCCHHHHHHHHHCCCH
VLGWRHMIDACLSEGLLVRATQASASHGGGYYVVSPNDRSQNQAARLFTRWVFEQAEAQM
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
GRAAS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]