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The map label for this gene is nuoF2 [H]

Identifier: 91782617

GI number: 91782617

Start: 1385745

End: 1387073

Strand: Direct

Name: nuoF2 [H]

Synonym: Bxe_A3209

Alternate gene names: 91782617

Gene position: 1385745-1387073 (Clockwise)

Preceding gene: 91782616

Following gene: 91782618

Centisome position: 28.3

GC content: 59.97

Gene sequence:

>1329_bases
ATGACGTGTTTACACGATCGTCACATCAAACCGCTGATTCTCGCCGGCCTGAACGGCGATAACTGGCATCTCGAAGACTA
TGTGGCGCGCGGCGGTTACGCCCAGCTGCGCCGTATTCTGGAAGAGAAGATTCCGCCCGAGCAGGTGATTGCCGACGTCA
AGGCGTCGGGCCTGCGTGGCCGGGGCGGTGCAGGCTTCCCGACCGGCCTGAAGTGGAGCTTCATGCCGCGTCAGTTCCCC
GGCCAGAAGTATCTCGTCTGCAATTCGGACGAAGGCGAACCGGGCACGTTCAAAGACCGCGATATTCTGCGTTTCAATCC
GCATTCGCTGATTGAAGGCATGGCCATTGGCGCGTACGCAATGGGCATTACGGTCGGCTACAACTATATTCACGGCGAAA
TCTGGGAAGTCTACAAACGCTTTGAACAGGCGTTGGACGAAGCTCGCCGCGCCGGGTTCCTTGGCGAAAACATCATGGGT
TCGGGCTTCTCGTTCGAACTGCATGCGCATCATGGTTACGGCGCCTATATCTGCGGCGAAGAAACCGCGCTGCTCGAATC
GCTGGAAGGCAAGAAAGGCCAGCCGCGCTTCAAGCCGCCATTCCCGGCGAGCTTCGGCGTGTACGGCAAGCCGACCACCA
TCAACAACACCGAGACGTTTGCCGCGGTGCCGTTCCTGCTCGCAATCGGTCCGCAGAATTACCTTGAAATCGGCAAGCCG
AATAACGGCGGCACGAAGATTTTTTCGATCGCAGGCGACGTCGAGCGTCCGGGCAATTATGAAATTCCGCTCGGCACGCC
GTTCGCCACGCTGATGGAACTCGCCGGCGGCATGCGCGGCGGCAAGAAGATCAAGGCCGTGATTCCTGGCGGCTCGTCGG
CGCCGGTCATTCCGGGCGACATCATGATGCAGACCGACATGGACTACGACTCGATCGCCAAGCAAGGCTCGATGCTCGGT
TCCGGCGCGGTCATCGTCATGGACGAGACGCGTTGCATGGTGCGCTCGTTGTTGCGTCTGTCGTATTTCTATTACGAGGA
ATCGTGTGGTCAATGCACGCCTTGCCGTGAAGGCACGGGCTGGCTGTACCGCGTCGTGCATCGTATCGAGCACGGACTCG
GCCGTCCGGAAGATCTGGATCTGCTGAACTCGGTCGCTGAAAACATCATGGGCCGCACGATTTGCGCGCTCGGCGATGCA
GCGGCCATGCCGGTTCGCGGCATGCTCAAGCACTACTGGGACGAATTCGAATATCACGTCGCCCACAAGCATTGCCTCGT
CGGCGGTCATGCCGGCGCTGCAGCGGCGTCGGAAACAGTGGCTGCCTGA

Upstream 100 bases:

>100_bases
GCTCGTGCGGCGACGCGCCGGTGATGCTGGTGAACAACCATCGCATGTGCAGCTTCATGAGCCGCGCGAAGATCGACCAG
CTGCTCGAGGAACTTTCGAA

Downstream 100 bases:

>100_bases
GCACGCAGACAGGTACGTAGAACAGGTACACACCAGCATACGTCAACCGCGGGATTCATCGCCTGAAACGGGCGATGAAC
AGGCGAACGATTGAGCGGTA

Product: putative NADH dehydrogenase I (chain F), NuoF

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTCLHDRHIKPLILAGLNGDNWHLEDYVARGGYAQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFP
GQKYLVCNSDEGEPGTFKDRDILRFNPHSLIEGMAIGAYAMGITVGYNYIHGEIWEVYKRFEQALDEARRAGFLGENIMG
SGFSFELHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAIGPQNYLEIGKP
NNGGTKIFSIAGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGDIMMQTDMDYDSIAKQGSMLG
SGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVHRIEHGLGRPEDLDLLNSVAENIMGRTICALGDA
AAMPVRGMLKHYWDEFEYHVAHKHCLVGGHAGAAAASETVAA

Sequences:

>Translated_442_residues
MTCLHDRHIKPLILAGLNGDNWHLEDYVARGGYAQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFP
GQKYLVCNSDEGEPGTFKDRDILRFNPHSLIEGMAIGAYAMGITVGYNYIHGEIWEVYKRFEQALDEARRAGFLGENIMG
SGFSFELHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAIGPQNYLEIGKP
NNGGTKIFSIAGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGDIMMQTDMDYDSIAKQGSMLG
SGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVHRIEHGLGRPEDLDLLNSVAENIMGRTICALGDA
AAMPVRGMLKHYWDEFEYHVAHKHCLVGGHAGAAAASETVAA
>Mature_441_residues
TCLHDRHIKPLILAGLNGDNWHLEDYVARGGYAQLRRILEEKIPPEQVIADVKASGLRGRGGAGFPTGLKWSFMPRQFPG
QKYLVCNSDEGEPGTFKDRDILRFNPHSLIEGMAIGAYAMGITVGYNYIHGEIWEVYKRFEQALDEARRAGFLGENIMGS
GFSFELHAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAIGPQNYLEIGKPN
NGGTKIFSIAGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGDIMMQTDMDYDSIAKQGSMLGS
GAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTGWLYRVVHRIEHGLGRPEDLDLLNSVAENIMGRTICALGDAA
AMPVRGMLKHYWDEFEYHVAHKHCLVGGHAGAAAASETVAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=400, Percent_Identity=48.25, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI260656005, Length=400, Percent_Identity=48.25, Blast_Score=382, Evalue=1e-106,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=43.2835820895522, Blast_Score=337, Evalue=9e-94,
Organism=Caenorhabditis elegans, GI17531727, Length=421, Percent_Identity=45.8432304038005, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19920794, Length=400, Percent_Identity=46.25, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45550452, Length=406, Percent_Identity=44.5812807881773, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24653819, Length=403, Percent_Identity=36.7245657568238, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24653817, Length=403, Percent_Identity=33.0024813895782, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48300; Mature: 48169

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCLHDRHIKPLILAGLNGDNWHLEDYVARGGYAQLRRILEEKIPPEQVIADVKASGLRG
CCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRQFPGQKYLVCNSDEGEPGTFKDRDILRFNPHSLIEGMAIGAYA
CCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHH
MGITVGYNYIHGEIWEVYKRFEQALDEARRAGFLGENIMGSGFSFELHAHHGYGAYICGE
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAIGPQNYLEIGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCHHEECCCC
NNGGTKIFSIAGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGD
CCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
IMMQTDMDYDSIAKQGSMLGSGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTG
EEEECCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
WLYRVVHRIEHGLGRPEDLDLLNSVAENIMGRTICALGDAAAMPVRGMLKHYWDEFEYHV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
AHKHCLVGGHAGAAAASETVAA
HHHEEEEECCCCCHHCHHHHCC
>Mature Secondary Structure 
TCLHDRHIKPLILAGLNGDNWHLEDYVARGGYAQLRRILEEKIPPEQVIADVKASGLRG
CCCCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
RGGAGFPTGLKWSFMPRQFPGQKYLVCNSDEGEPGTFKDRDILRFNPHSLIEGMAIGAYA
CCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHH
MGITVGYNYIHGEIWEVYKRFEQALDEARRAGFLGENIMGSGFSFELHAHHGYGAYICGE
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEECC
ETALLESLEGKKGQPRFKPPFPASFGVYGKPTTINNTETFAAVPFLLAIGPQNYLEIGKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCHHEECCCC
NNGGTKIFSIAGDVERPGNYEIPLGTPFATLMELAGGMRGGKKIKAVIPGGSSAPVIPGD
CCCCCEEEEEECCCCCCCCEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
IMMQTDMDYDSIAKQGSMLGSGAVIVMDETRCMVRSLLRLSYFYYEESCGQCTPCREGTG
EEEECCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
WLYRVVHRIEHGLGRPEDLDLLNSVAENIMGRTICALGDAAAMPVRGMLKHYWDEFEYHV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHH
AHKHCLVGGHAGAAAASETVAA
HHHEEEEECCCCCHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]