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The map label for this gene is potB [H]

Identifier: 91782360

GI number: 91782360

Start: 1096341

End: 1097594

Strand: Reverse

Name: potB [H]

Synonym: Bxe_A3471

Alternate gene names: 91782360

Gene position: 1097594-1096341 (Counterclockwise)

Preceding gene: 91782361

Following gene: 91782359

Centisome position: 22.42

GC content: 66.83

Gene sequence:

>1254_bases
ATGCCCGCTCCGGCCCGCGCCACAGCCGTGGGCTCTCCGACGAGAGCCGACGGCGGCGCGTCATTTCAGAAGGCGCGCCG
CCGCGCATCCGTGCGGGCGCTGTTGCTCGCGCTGCCGCTCATCGTGTTCCTCGTTTCGACGTTCATCGCGCCGATCGCGC
TGCTGCTCGCGCGCAGCGTGCAGAACCACGAGATGTCCAACGGCATGCCGGCGCTCACGCGCGCGCTCGACGCGTGGGAC
GGCCGCGGCGTGCCGGACGAGCACATGTTCGCGCTGCTCGCCGCGGGGCTCCGCGAGGCGCAGCAGAGCGGTCAGCTCGG
CACCGTCGCGCGGCGGCTGAACTTCGATCAGGCCGAATTCCGCAGCCTGTTGATGCGCACCGCGCGCCAACTTCCCGCCG
ATGCGCCGCCCGCGTGGAAACCGGCGCTCATCGAACTCGACGAACGCTGGAATTCGCCGGAAATCTGGCGCCTGCTGAAG
CGCGCCGCCGCGTCGCCGACGCCGGACTATCTGCTCGCCGCCGTCGATGCGCAAGTCACGCCGCAAGGCTCGGTCGCGTT
CGTGCCGGACAACGCGTCGATCTATCGGCAGGCCTTTGCGCGCACGATCTCGATCAGCGCCACGGTCACGCTGCTGTGCC
TCGTGCTCGGCTATCCGGTTGCATGGCTGCTCGCGAACCTGCCCGCGAAAAGCAGCAACCGCCTGATGCTGTTCGTGATC
GTGCCGTTCTGGACCTCGCTGCTCGTGCGCACGACCGCATGGTACGTTCTGCTGCAACCGGGCGGTGTCATCAACAGTCT
GCTGACGGGTCTCGGGCTCGCCACGCATCCGCTGCCGCTCATCTTCAACCGCACGGGTGTGCTGATCGGCATGACGCACG
TGCTCCTGCCGTACATGATTCTCGCCATCTATTCGGTGATGAAAGGGGTATCGCCCGTCTACGTGCGGGCCGCGCAATCG
CTCGGCGCGCATCCGTTCACCGCGTTCGTGCGCGTCTACATTCCGCAGACGCTGCCCGGCGTGGGCGCAGGCTGCTTCCT
CGTGTTCGTGCTCGCGCTCGGCTACTACATCACGCCTGCGCTGCTCGGCGGCGCTGGCGACGAGATGATCAGCCAGCTCA
TCGCGATCCAGACCAATACGCAACTCAACTGGGGGCTCGCCGGCGCCCTCTCGGCCTATCTCGTGATCTTCACGGCGATC
TTCTATTTCCTGTTCAACCGCATTGTCGGTATCGACCGCTTGCGCTTCGGTTGA

Upstream 100 bases:

>100_bases
ACCTTCTGGGCCGACAACGCGGACCAGATCAACAAGCGCTTCCAGGTGTGGCTGACGCAGTAACCCCATCGTCCGACGAG
AGCCCAACGTGACTACCAGC

Downstream 100 bases:

>100_bases
GCCCCGGTCGAAACACGCGCACACGACGAAGGAACGACATGCAAGAACAACGCAACAAGGTGCTGACGGAACGCATCGCC
ACGCGCTGGGTGCGACTGCA

Product: ABC spermidine/putrescine transporter, innermembrane subunit

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MPAPARATAVGSPTRADGGASFQKARRRASVRALLLALPLIVFLVSTFIAPIALLLARSVQNHEMSNGMPALTRALDAWD
GRGVPDEHMFALLAAGLREAQQSGQLGTVARRLNFDQAEFRSLLMRTARQLPADAPPAWKPALIELDERWNSPEIWRLLK
RAAASPTPDYLLAAVDAQVTPQGSVAFVPDNASIYRQAFARTISISATVTLLCLVLGYPVAWLLANLPAKSSNRLMLFVI
VPFWTSLLVRTTAWYVLLQPGGVINSLLTGLGLATHPLPLIFNRTGVLIGMTHVLLPYMILAIYSVMKGVSPVYVRAAQS
LGAHPFTAFVRVYIPQTLPGVGAGCFLVFVLALGYYITPALLGGAGDEMISQLIAIQTNTQLNWGLAGALSAYLVIFTAI
FYFLFNRIVGIDRLRFG

Sequences:

>Translated_417_residues
MPAPARATAVGSPTRADGGASFQKARRRASVRALLLALPLIVFLVSTFIAPIALLLARSVQNHEMSNGMPALTRALDAWD
GRGVPDEHMFALLAAGLREAQQSGQLGTVARRLNFDQAEFRSLLMRTARQLPADAPPAWKPALIELDERWNSPEIWRLLK
RAAASPTPDYLLAAVDAQVTPQGSVAFVPDNASIYRQAFARTISISATVTLLCLVLGYPVAWLLANLPAKSSNRLMLFVI
VPFWTSLLVRTTAWYVLLQPGGVINSLLTGLGLATHPLPLIFNRTGVLIGMTHVLLPYMILAIYSVMKGVSPVYVRAAQS
LGAHPFTAFVRVYIPQTLPGVGAGCFLVFVLALGYYITPALLGGAGDEMISQLIAIQTNTQLNWGLAGALSAYLVIFTAI
FYFLFNRIVGIDRLRFG
>Mature_416_residues
PAPARATAVGSPTRADGGASFQKARRRASVRALLLALPLIVFLVSTFIAPIALLLARSVQNHEMSNGMPALTRALDAWDG
RGVPDEHMFALLAAGLREAQQSGQLGTVARRLNFDQAEFRSLLMRTARQLPADAPPAWKPALIELDERWNSPEIWRLLKR
AAASPTPDYLLAAVDAQVTPQGSVAFVPDNASIYRQAFARTISISATVTLLCLVLGYPVAWLLANLPAKSSNRLMLFVIV
PFWTSLLVRTTAWYVLLQPGGVINSLLTGLGLATHPLPLIFNRTGVLIGMTHVLLPYMILAIYSVMKGVSPVYVRAAQSL
GAHPFTAFVRVYIPQTLPGVGAGCFLVFVLALGYYITPALLGGAGDEMISQLIAIQTNTQLNWGLAGALSAYLVIFTAIF
YFLFNRIVGIDRLRFG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=229, Percent_Identity=34.061135371179, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787080, Length=253, Percent_Identity=32.0158102766798, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787713, Length=262, Percent_Identity=29.0076335877863, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45164; Mature: 45033

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPARATAVGSPTRADGGASFQKARRRASVRALLLALPLIVFLVSTFIAPIALLLARSV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNHEMSNGMPALTRALDAWDGRGVPDEHMFALLAAGLREAQQSGQLGTVARRLNFDQAEF
HHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
RSLLMRTARQLPADAPPAWKPALIELDERWNSPEIWRLLKRAAASPTPDYLLAAVDAQVT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
PQGSVAFVPDNASIYRQAFARTISISATVTLLCLVLGYPVAWLLANLPAKSSNRLMLFVI
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
VPFWTSLLVRTTAWYVLLQPGGVINSLLTGLGLATHPLPLIFNRTGVLIGMTHVLLPYMI
HHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHH
LAIYSVMKGVSPVYVRAAQSLGAHPFTAFVRVYIPQTLPGVGAGCFLVFVLALGYYITPA
HHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLGGAGDEMISQLIAIQTNTQLNWGLAGALSAYLVIFTAIFYFLFNRIVGIDRLRFG
HHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PAPARATAVGSPTRADGGASFQKARRRASVRALLLALPLIVFLVSTFIAPIALLLARSV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNHEMSNGMPALTRALDAWDGRGVPDEHMFALLAAGLREAQQSGQLGTVARRLNFDQAEF
HHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
RSLLMRTARQLPADAPPAWKPALIELDERWNSPEIWRLLKRAAASPTPDYLLAAVDAQVT
HHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
PQGSVAFVPDNASIYRQAFARTISISATVTLLCLVLGYPVAWLLANLPAKSSNRLMLFVI
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
VPFWTSLLVRTTAWYVLLQPGGVINSLLTGLGLATHPLPLIFNRTGVLIGMTHVLLPYMI
HHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHH
LAIYSVMKGVSPVYVRAAQSLGAHPFTAFVRVYIPQTLPGVGAGCFLVFVLALGYYITPA
HHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LLGGAGDEMISQLIAIQTNTQLNWGLAGALSAYLVIFTAIFYFLFNRIVGIDRLRFG
HHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]