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The map label for this gene is potC [H]

Identifier: 91782359

GI number: 91782359

Start: 1095475

End: 1096302

Strand: Reverse

Name: potC [H]

Synonym: Bxe_A3472

Alternate gene names: 91782359

Gene position: 1096302-1095475 (Counterclockwise)

Preceding gene: 91782360

Following gene: 91782357

Centisome position: 22.39

GC content: 63.77

Gene sequence:

>828_bases
ATGCAAGAACAACGCAACAAGGTGCTGACGGAACGCATCGCCACGCGCTGGGTGCGACTGCATACAGCGGTCGTGCTCTT
CTTTCTGATTGCGCCGATTCTCGCCATCATCCCGCTCTCGTTCAATTCGGGCTCGTATTTCTCGTACCCGATGCAGGGCT
TTTCGTTTCGCTGGTATGAGCAGGCGCTGACGAGCGCGGACTGGCAACGGTCGCTTCTGAACAGCATCGGCATCGGCGCG
GCGTCCACACTGATCGCCACGTGTCTGGGCACGCTCGCCGCGCTGGGCCTGTCGCGCGCGCAGTTTCCGCTGCGCTCGCT
GATCATGCCGATCATCATTTCGCCGATGATCGTGCCGATCGTGGTCGTTGCCGCGGGCTTCTATCTGATCTTCGCGCCGC
TCGGCCTCGTGAACTCGTATCCCGGCGTGGTGCTCGCGCACGCGGCGCTCGGCACGCCGTTCGTGGTGATCACCGTGACG
GCGTCGCTGCTTTCGTTCGATCAAAGTCTGCTGCGCGCCGCATCCGGTCTGGGCGCAGCGCCCTGGGTCACGTTCCGGCG
CGTTACGCTGCCGCTCATCATGCCCGCCGTCGCCACCGGCAGCGTCTTCGCTTTCGCGACTTCGTTCGATGAAGTGATCG
TCATTCTGTTCATTGGCGGCCCGGATCAAACCACCGTGCCGCGGCAGATGTGGAGCGGCATTCGCGATTCGATCGACCCA
TCGATTCTCGCCGTGGCGACCATGCTGATCGTATTCGCGGTGCTGCTCTTCGCGAGCATCAACTGGCTGCGCGGCCGCGC
GGCCGCGGCCAGCAGCGCGCTTACCTGA

Upstream 100 bases:

>100_bases
CGGCGATCTTCTATTTCCTGTTCAACCGCATTGTCGGTATCGACCGCTTGCGCTTCGGTTGAGCCCCGGTCGAAACACGC
GCACACGACGAAGGAACGAC

Downstream 100 bases:

>100_bases
CGCTCGGGGCCACGCGCTGCCCGGCCGCTCCGGCAATCCGGGTATCGCGACGCGGGGCTGCGTTTCCGGTCAGGGGTTGG
TCTCCGCCGGTAAGCGCATC

Product: ABC spermidine/putrescine transporter, innermembrane subunit

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MQEQRNKVLTERIATRWVRLHTAVVLFFLIAPILAIIPLSFNSGSYFSYPMQGFSFRWYEQALTSADWQRSLLNSIGIGA
ASTLIATCLGTLAALGLSRAQFPLRSLIMPIIISPMIVPIVVVAAGFYLIFAPLGLVNSYPGVVLAHAALGTPFVVITVT
ASLLSFDQSLLRAASGLGAAPWVTFRRVTLPLIMPAVATGSVFAFATSFDEVIVILFIGGPDQTTVPRQMWSGIRDSIDP
SILAVATMLIVFAVLLFASINWLRGRAAAASSALT

Sequences:

>Translated_275_residues
MQEQRNKVLTERIATRWVRLHTAVVLFFLIAPILAIIPLSFNSGSYFSYPMQGFSFRWYEQALTSADWQRSLLNSIGIGA
ASTLIATCLGTLAALGLSRAQFPLRSLIMPIIISPMIVPIVVVAAGFYLIFAPLGLVNSYPGVVLAHAALGTPFVVITVT
ASLLSFDQSLLRAASGLGAAPWVTFRRVTLPLIMPAVATGSVFAFATSFDEVIVILFIGGPDQTTVPRQMWSGIRDSIDP
SILAVATMLIVFAVLLFASINWLRGRAAAASSALT
>Mature_275_residues
MQEQRNKVLTERIATRWVRLHTAVVLFFLIAPILAIIPLSFNSGSYFSYPMQGFSFRWYEQALTSADWQRSLLNSIGIGA
ASTLIATCLGTLAALGLSRAQFPLRSLIMPIIISPMIVPIVVVAAGFYLIFAPLGLVNSYPGVVLAHAALGTPFVVITVT
ASLLSFDQSLLRAASGLGAAPWVTFRRVTLPLIMPAVATGSVFAFATSFDEVIVILFIGGPDQTTVPRQMWSGIRDSIDP
SILAVATMLIVFAVLLFASINWLRGRAAAASSALT

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=217, Percent_Identity=32.7188940092166, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787081, Length=242, Percent_Identity=33.4710743801653, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787714, Length=198, Percent_Identity=35.8585858585859, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29546; Mature: 29546

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEQRNKVLTERIATRWVRLHTAVVLFFLIAPILAIIPLSFNSGSYFSYPMQGFSFRWYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
QALTSADWQRSLLNSIGIGAASTLIATCLGTLAALGLSRAQFPLRSLIMPIIISPMIVPI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVVAAGFYLIFAPLGLVNSYPGVVLAHAALGTPFVVITVTASLLSFDQSLLRAASGLGAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCCCC
PWVTFRRVTLPLIMPAVATGSVFAFATSFDEVIVILFIGGPDQTTVPRQMWSGIRDSIDP
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCH
SILAVATMLIVFAVLLFASINWLRGRAAAASSALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQEQRNKVLTERIATRWVRLHTAVVLFFLIAPILAIIPLSFNSGSYFSYPMQGFSFRWYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
QALTSADWQRSLLNSIGIGAASTLIATCLGTLAALGLSRAQFPLRSLIMPIIISPMIVPI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVVAAGFYLIFAPLGLVNSYPGVVLAHAALGTPFVVITVTASLLSFDQSLLRAASGLGAA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCCCC
PWVTFRRVTLPLIMPAVATGSVFAFATSFDEVIVILFIGGPDQTTVPRQMWSGIRDSIDP
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCH
SILAVATMLIVFAVLLFASINWLRGRAAAASSALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]