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The map label for this gene is pobB [H]

Identifier: 91782297

GI number: 91782297

Start: 1025534

End: 1026499

Strand: Direct

Name: pobB [H]

Synonym: Bxe_A3536

Alternate gene names: 91782297

Gene position: 1025534-1026499 (Clockwise)

Preceding gene: 91782296

Following gene: 91782298

Centisome position: 20.95

GC content: 65.42

Gene sequence:

>966_bases
ATGTCCCAGCATTCCCCTGAACGCATCGACGTCCACCTCACGCAAATCCGCCTTGAAGCGGACGACGTGGCTTCCTACGA
GTTCCGGCCGGTGGCCGGCGGCCCGGCGTTGCCTGTCTTTGAGGCTGGCGCGCACATCGACCTTTACCTGCCGGAAAACC
GCGTGCGCAGCTATTCGCTCGTCAACGATCCGCAGGAACGGCACCGCTACGTGGTCGCCGTGCAGCGCGAGGCCGCCGGC
CGCGGCGGCTCCGCGTGGATGCATCGCGTGCCGCGGGTCGGCGAGCGCTTCACGATCAGCGCACCGAAGAACGATTTTCC
GTTGTGCGAGAGCGCACGCGAATCCCTATTCATCTGCGGCGGGATCGGCATAACGCCGGTCGTCGCGATGATCCGGCGGC
TAGAGCGCATCGGCGCGTCGTGGCGTTTGCATTACGCAGTGCGGGAGTGTTCCCGTGCAGCCTATGCGGATGATCTCGCG
CAGCTCGACAACGCGGATTCGCGAGTCGAAATGTACTTCCAGAACGAAGACCGACGGCCGGACGTGCGCGGCATCGTCGA
TAGGGCGCCCGCCGGCACGCATCTGTATTGCTGCGGTCCGCGCGGCATGGTGGACGATTTCATCGCCGCGTGCGCGGGAC
GTCCGCAGGACGAAGTGCATTTCGAGCGTTTCGCCGCCGCGAACGACGCGGCGAGCGAAGGTGGCTTCGAAGTCGTATTG
CACCGCGATGGCCGGCGTATTGCCGTTGCGCCCGGAAAGACCATTCTGGACACTTTGCTCGATCACGGTGTCGACGTGCA
GTACGCGTGTTCGTCGGGCGTGTGCGGAACCTGCCGCGTCGGCGTGATCGACGGCGTGCCCGATCATCGCGACGACTTCC
TGAGCGCCGACGAGAAGGCCGGCAACGGCGCGATCATGGTGTGCTGCTCGGGCTCGCTTTCGCCGACGCTGGTGCTCGAT
CTCTGA

Upstream 100 bases:

>100_bases
CGTCGTGGCAGAACGACGGCGCTGTCCACATGCGTGCGCGCCTCGGCGCGGACTTCGGCTACAAGCCCTGACCCTCCTTC
GCCTGCTAATTCGAGTCAAC

Downstream 100 bases:

>100_bases
GCACGCGCCGCGCACGCAAACGAATACGAACTCACCGGCAGGCGCCGCCGCGCGCCGCCTCGACAAGGAGACAAGATGCT
GTCGAATCTGGTCAGTGAAT

Product: aromatic acid dioxygenase beta subunit

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: 4-carboxydiphenyl ether; phenoxybenzoate dioxygenase subunit beta [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSQHSPERIDVHLTQIRLEADDVASYEFRPVAGGPALPVFEAGAHIDLYLPENRVRSYSLVNDPQERHRYVVAVQREAAG
RGGSAWMHRVPRVGERFTISAPKNDFPLCESARESLFICGGIGITPVVAMIRRLERIGASWRLHYAVRECSRAAYADDLA
QLDNADSRVEMYFQNEDRRPDVRGIVDRAPAGTHLYCCGPRGMVDDFIAACAGRPQDEVHFERFAAANDAASEGGFEVVL
HRDGRRIAVAPGKTILDTLLDHGVDVQYACSSGVCGTCRVGVIDGVPDHRDDFLSADEKAGNGAIMVCCSGSLSPTLVLD
L

Sequences:

>Translated_321_residues
MSQHSPERIDVHLTQIRLEADDVASYEFRPVAGGPALPVFEAGAHIDLYLPENRVRSYSLVNDPQERHRYVVAVQREAAG
RGGSAWMHRVPRVGERFTISAPKNDFPLCESARESLFICGGIGITPVVAMIRRLERIGASWRLHYAVRECSRAAYADDLA
QLDNADSRVEMYFQNEDRRPDVRGIVDRAPAGTHLYCCGPRGMVDDFIAACAGRPQDEVHFERFAAANDAASEGGFEVVL
HRDGRRIAVAPGKTILDTLLDHGVDVQYACSSGVCGTCRVGVIDGVPDHRDDFLSADEKAGNGAIMVCCSGSLSPTLVLD
L
>Mature_320_residues
SQHSPERIDVHLTQIRLEADDVASYEFRPVAGGPALPVFEAGAHIDLYLPENRVRSYSLVNDPQERHRYVVAVQREAAGR
GGSAWMHRVPRVGERFTISAPKNDFPLCESARESLFICGGIGITPVVAMIRRLERIGASWRLHYAVRECSRAAYADDLAQ
LDNADSRVEMYFQNEDRRPDVRGIVDRAPAGTHLYCCGPRGMVDDFIAACAGRPQDEVHFERFAAANDAASEGGFEVVLH
RDGRRIAVAPGKTILDTLLDHGVDVQYACSSGVCGTCRVGVIDGVPDHRDDFLSADEKAGNGAIMVCCSGSLSPTLVLDL

Specific function: Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=321, Percent_Identity=33.0218068535826, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787658, Length=319, Percent_Identity=26.9592476489028, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787098, Length=319, Percent_Identity=23.1974921630094, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788903, Length=230, Percent_Identity=26.9565217391304, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 35107; Mature: 34976

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHSPERIDVHLTQIRLEADDVASYEFRPVAGGPALPVFEAGAHIDLYLPENRVRSYSL
CCCCCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCCCEEECCCEEEEEECCCCCCEEEC
VNDPQERHRYVVAVQREAAGRGGSAWMHRVPRVGERFTISAPKNDFPLCESARESLFICG
CCCHHHHCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCHHCCCCEEEEC
GIGITPVVAMIRRLERIGASWRLHYAVRECSRAAYADDLAQLDNADSRVEMYFQNEDRRP
CCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
DVRGIVDRAPAGTHLYCCGPRGMVDDFIAACAGRPQDEVHFERFAAANDAASEGGFEVVL
CHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
HRDGRRIAVAPGKTILDTLLDHGVDVQYACSSGVCGTCRVGVIDGVPDHRDDFLSADEKA
EECCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHCCCCCCC
GNGAIMVCCSGSLSPTLVLDL
CCCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SQHSPERIDVHLTQIRLEADDVASYEFRPVAGGPALPVFEAGAHIDLYLPENRVRSYSL
CCCCCCEEEEEEEEEEEECCCCCCEEEEECCCCCCCCEEECCCEEEEEECCCCCCEEEC
VNDPQERHRYVVAVQREAAGRGGSAWMHRVPRVGERFTISAPKNDFPLCESARESLFICG
CCCHHHHCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCHHCCCCEEEEC
GIGITPVVAMIRRLERIGASWRLHYAVRECSRAAYADDLAQLDNADSRVEMYFQNEDRRP
CCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
DVRGIVDRAPAGTHLYCCGPRGMVDDFIAACAGRPQDEVHFERFAAANDAASEGGFEVVL
CHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
HRDGRRIAVAPGKTILDTLLDHGVDVQYACSSGVCGTCRVGVIDGVPDHRDDFLSADEKA
EECCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHCCCCCCC
GNGAIMVCCSGSLSPTLVLDL
CCCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7710319 [H]