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The map label for this gene is cbaA [H]

Identifier: 91782296

GI number: 91782296

Start: 1024182

End: 1025504

Strand: Direct

Name: cbaA [H]

Synonym: Bxe_A3537

Alternate gene names: 91782296

Gene position: 1024182-1025504 (Clockwise)

Preceding gene: 91782291

Following gene: 91782297

Centisome position: 20.92

GC content: 63.79

Gene sequence:

>1323_bases
ATGCTTTCCCATCAAGACAACGAAATCCTTGTACGCGTTGGCCCCGGCACGCCGACCGGGCAGTTCATGCGCCTTTTCTG
GTTGCCGTTCCTGCCGTCGTCGGATCTCTCGGTCGACGGGCAGCCGCAACGCGTGCGTCTGCTCGGCGAAGACCTGCTCG
CGTTTCGCGACTCGGAAGGCCGCGTCGGTCTGGTCGACCATGCGTGCCCGCATCGAGGTGCGCCGCTTGTATTCGGTCGC
AATGAAAATAGCGGCTTGCGCTGCGTCTATCACGGCTGGCAGTTCGGCGTGGACGGCCGCGTGATCGAAACGCCGGCCGA
GCCGATCGAGAGCCGGCTGAAAGAGAAAGTGCGCATCAAGAGCTATCCGTGCCGCGAGCGCAACGGGATCGTGTGGACCT
ACATGGGCGATGCGTCCGCGGACGGACTGCCGCCGCTGCCGAATCTCGAATGGAACATGGTGCCGGTGGAAAACGTGCAC
TTCAGCTTCCGCGTGCAGGAGTGCAACTGGCTGCAGGCTGTGGAAGGCGAGATCGATTCGGCGCACGCACCGATCCTGCA
TGGACGCATCGATTCGCAGGGGGCGATCAACGAATGGACCGCGAAGCGCGACCTGCGGCCCGTGTTCGAATGTCAGCGGC
AGGATTTCGGCATCAGCATCGCTTCGCGGCGCGTGCTTGATGAAGAGCATCACTACTGGCGCGTCAACCAGTTCCTGTTC
CCGTTCTACACACTGGTGCCGCCGCAGTCGAAGTATCCGGAGCTGAGCGGTCACGCATGGGTGCCGATCGACGACGAGAA
CACGTTGTGCTTGATGTTCTCTTATACGCCGGCTACCCCGCTGTACGAGAAGACGAGACAACTGTTCGAACAGGGGCATC
AGGGCCGCGAGACGGGCCACGCGAGCGTGAACGCGTACGAGCCGCGCCCGGTGACGAATCCGTACGCGAAGTACTGGACC
CGATACAACCTCGAATCGGCGTTCCAGTTCGATTACGACGCGCAAACGAAGACGTGGTTCTCCGGACTGCCGGGGCTGTG
GGTGCAGGATGCCGCCTGCCAGTCGGGCGTCGCGCCGATCTACGACCGCAGCAAGGAGAACCTCGGCATCAGCGACACGG
GCGTCGCGATGACGCGCCGCCTGCTACTCGAAAACGTGCGCAAACTCGCGGCCGAGCGCGTGTCGACGGTGCAGGTCGAC
GAGCCGGATCTGTGGATGGTCCGCGCGGTGTCGCTAACGCTGCAGGCCGGCGCGTCGTGGCAGAACGACGGCGCTGTCCA
CATGCGTGCGCGCCTCGGCGCGGACTTCGGCTACAAGCCCTGA

Upstream 100 bases:

>100_bases
CATGCAGGGAACATCCTTCCGAGACTGAAGACCCGGTTGACGGTCCTGTCGATGGCTCTATAATTGGCCCTACCAATTTC
AACGAGATACTGGACCCGAC

Downstream 100 bases:

>100_bases
CCCTCCTTCGCCTGCTAATTCGAGTCAACATGTCCCAGCATTCCCCTGAACGCATCGACGTCCACCTCACGCAAATCCGC
CTTGAAGCGGACGACGTGGC

Product: aromatic acid dioxygenase alpha subunit

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLSHQDNEILVRVGPGTPTGQFMRLFWLPFLPSSDLSVDGQPQRVRLLGEDLLAFRDSEGRVGLVDHACPHRGAPLVFGR
NENSGLRCVYHGWQFGVDGRVIETPAEPIESRLKEKVRIKSYPCRERNGIVWTYMGDASADGLPPLPNLEWNMVPVENVH
FSFRVQECNWLQAVEGEIDSAHAPILHGRIDSQGAINEWTAKRDLRPVFECQRQDFGISIASRRVLDEEHHYWRVNQFLF
PFYTLVPPQSKYPELSGHAWVPIDDENTLCLMFSYTPATPLYEKTRQLFEQGHQGRETGHASVNAYEPRPVTNPYAKYWT
RYNLESAFQFDYDAQTKTWFSGLPGLWVQDAACQSGVAPIYDRSKENLGISDTGVAMTRRLLLENVRKLAAERVSTVQVD
EPDLWMVRAVSLTLQAGASWQNDGAVHMRARLGADFGYKP

Sequences:

>Translated_440_residues
MLSHQDNEILVRVGPGTPTGQFMRLFWLPFLPSSDLSVDGQPQRVRLLGEDLLAFRDSEGRVGLVDHACPHRGAPLVFGR
NENSGLRCVYHGWQFGVDGRVIETPAEPIESRLKEKVRIKSYPCRERNGIVWTYMGDASADGLPPLPNLEWNMVPVENVH
FSFRVQECNWLQAVEGEIDSAHAPILHGRIDSQGAINEWTAKRDLRPVFECQRQDFGISIASRRVLDEEHHYWRVNQFLF
PFYTLVPPQSKYPELSGHAWVPIDDENTLCLMFSYTPATPLYEKTRQLFEQGHQGRETGHASVNAYEPRPVTNPYAKYWT
RYNLESAFQFDYDAQTKTWFSGLPGLWVQDAACQSGVAPIYDRSKENLGISDTGVAMTRRLLLENVRKLAAERVSTVQVD
EPDLWMVRAVSLTLQAGASWQNDGAVHMRARLGADFGYKP
>Mature_440_residues
MLSHQDNEILVRVGPGTPTGQFMRLFWLPFLPSSDLSVDGQPQRVRLLGEDLLAFRDSEGRVGLVDHACPHRGAPLVFGR
NENSGLRCVYHGWQFGVDGRVIETPAEPIESRLKEKVRIKSYPCRERNGIVWTYMGDASADGLPPLPNLEWNMVPVENVH
FSFRVQECNWLQAVEGEIDSAHAPILHGRIDSQGAINEWTAKRDLRPVFECQRQDFGISIASRRVLDEEHHYWRVNQFLF
PFYTLVPPQSKYPELSGHAWVPIDDENTLCLMFSYTPATPLYEKTRQLFEQGHQGRETGHASVNAYEPRPVTNPYAKYWT
RYNLESAFQFDYDAQTKTWFSGLPGLWVQDAACQSGVAPIYDRSKENLGISDTGVAMTRRLLLENVRKLAAERVSTVQVD
EPDLWMVRAVSLTLQAGASWQNDGAVHMRARLGADFGYKP

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=194, Percent_Identity=27.8350515463918, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 50004; Mature: 50004

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHQDNEILVRVGPGTPTGQFMRLFWLPFLPSSDLSVDGQPQRVRLLGEDLLAFRDSEG
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHEEHHHHHHHHEECCCC
RVGLVDHACPHRGAPLVFGRNENSGLRCVYHGWQFGVDGRVIETPAEPIESRLKEKVRIK
CEEEECCCCCCCCCCEEEECCCCCCEEEEEECEEECCCCEEEECCHHHHHHHHHHHHHHC
SYPCRERNGIVWTYMGDASADGLPPLPNLEWNMVPVENVHFSFRVQECNWLQAVEGEIDS
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHCCCCCC
AHAPILHGRIDSQGAINEWTAKRDLRPVFECQRQDFGISIASRRVLDEEHHYWRVNQFLF
CCCCEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCCEEHHHHHHHCCCCCEEEEHHHHC
PFYTLVPPQSKYPELSGHAWVPIDDENTLCLMFSYTPATPLYEKTRQLFEQGHQGRETGH
CHHEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCC
ASVNAYEPRPVTNPYAKYWTRYNLESAFQFDYDAQTKTWFSGLPGLWVQDAACQSGVAPI
CCEEEECCCCCCCHHHHHHEECCCCCEEEECCCCCCHHHHCCCCCCEEECHHHHCCCCCH
YDRSKENLGISDTGVAMTRRLLLENVRKLAAERVSTVQVDEPDLWMVRAVSLTLQAGASW
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEEEEEEEEECCCCC
QNDGAVHMRARLGADFGYKP
CCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MLSHQDNEILVRVGPGTPTGQFMRLFWLPFLPSSDLSVDGQPQRVRLLGEDLLAFRDSEG
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHEEHHHHHHHHEECCCC
RVGLVDHACPHRGAPLVFGRNENSGLRCVYHGWQFGVDGRVIETPAEPIESRLKEKVRIK
CEEEECCCCCCCCCCEEEECCCCCCEEEEEECEEECCCCEEEECCHHHHHHHHHHHHHHC
SYPCRERNGIVWTYMGDASADGLPPLPNLEWNMVPVENVHFSFRVQECNWLQAVEGEIDS
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHCCCCCC
AHAPILHGRIDSQGAINEWTAKRDLRPVFECQRQDFGISIASRRVLDEEHHYWRVNQFLF
CCCCEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCCEEHHHHHHHCCCCCEEEEHHHHC
PFYTLVPPQSKYPELSGHAWVPIDDENTLCLMFSYTPATPLYEKTRQLFEQGHQGRETGH
CHHEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCC
ASVNAYEPRPVTNPYAKYWTRYNLESAFQFDYDAQTKTWFSGLPGLWVQDAACQSGVAPI
CCEEEECCCCCCCHHHHHHEECCCCCEEEECCCCCCHHHHCCCCCCEEECHHHHCCCCCH
YDRSKENLGISDTGVAMTRRLLLENVRKLAAERVSTVQVDEPDLWMVRAVSLTLQAGASW
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEEEEEEEEECCCCC
QNDGAVHMRARLGADFGYKP
CCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704279 [H]