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The map label for this gene is cbdA [H]

Identifier: 91782278

GI number: 91782278

Start: 1006454

End: 1007758

Strand: Direct

Name: cbdA [H]

Synonym: Bxe_A3556

Alternate gene names: 91782278

Gene position: 1006454-1007758 (Clockwise)

Preceding gene: 91782277

Following gene: 91782279

Centisome position: 20.56

GC content: 59.39

Gene sequence:

>1305_bases
ATGGAGAATGAGTCGAAGTTGGTGGAGGTAGACGAAAAGCACCTCCAGTTCCGGGTGGCCCGGGAAGCATTCGTCAGTGA
GCAGATCCTGTCCGGTGAGTACGAAAAGATCTTCGACCGGTGCTGGCTGTACGTCGGGCACGTGTCCGAGTTCAGCAAGC
CCGGTGATTTCGTCACACGTACCATTGCGCGACGCAACCTGCTCGTAACGATGGGAACGGACAACAGGATCAATGCGTTC
TTTAACACCTGCCCCCACCGCGGGGCGACGGTTTGTCGGGATCGGGCCGGCAATTCGAAGAACTTCCAGTGCTTCTATCA
CGGCTGGGTATTCGGCTGCGACGGCAACCTCAAGAGCCAGCCGGGCAAGGACCGGTATTGCGCCGACTTCATCGCGAGCG
GAGCGGGCAATCTCGTATCCGTACCGCGATTCGACATCTATGCGGGTTTCTGCTTCGTCAGCTTCAACGCAGAGGTCGAG
CCGTTACCCGACTATCTGGCGGGCGCGAAGGAATACCTCGACCTGGTGTCGAGGTATTCCGAGAGTGGCATGGCGATTTC
CAGTGGCACGCAGGAGTACGCGATCCGTGCGAACTGGAAACTGCTGGTGGAAAACAGCGTCGATGGCTACCACGCGGTGA
GCACGCATGCTACCTACCTCGATTATCTGAAGAACACCAATGGCAGCCTGTCCAGTACGAAACTCGAGGGGACGAGCTAC
GATCTGGGCAATGGTCACGCGGTGCTCGAATACACAGCCCCCTGGGGGCGCCCGATTGCTTCGTGGGTACCGCTCTGGGG
TGAGCAGGGGAAGAAGGAAATCGACCAGATATATGCCCGCCTTGTCGAGCTTCATGGCGAGGACATGGCTGATCGTATGG
CGCACAAGAACCGGAACCTGCTGATTTTTCCGAACCTTGTCGTCAACGACATCATGGCCATCACGGTCCGGACGTTCTAC
CCACAGGCACCGAACTACATGCTGGTCAATGGCTGGTCGCTCGCACCCAGAGAGGAGTCTGAGTGGGCGAAGAAGTTCCG
GCTCAGCAACTTTCTCGAGTTCCTCGGGCCGGGCGGATTCGCCACGCCCGATGACGTCGAGGCGCTGGAGTCCTGCCAGA
ACGGTTTCAGCAACTATCGGCTGGTGCCGTGGAGCGACATCTCCAAGGGCATGGGCAAGGCGTCGCCCAACTACGACGAC
GAACTACAGATGCGCGCGTTCTGGACACGCTGGAATCAGTACATCAGCGGCTCGCCGTCTCCGCAGGCGACCATCCGCCG
GCTCCCGTCAGCAACCGCAGCCTGA

Upstream 100 bases:

>100_bases
AGGGAAATCGGGTGGATCCGCATCAGCTCGCCGAGCCGGGCACGCCATTACGCAATTGTGCGCTTTGAAGGCACAGGTTC
AGAACCACGAGGATAAGACC

Downstream 100 bases:

>100_bases
GGATACGCATATGAGCGCCGTTACCATCGGGAAGGACCCCGTTCGAATCCCGTCTTCCAGCGACTTCGCGGAATACGTGA
CGAGAACGGACGTAGAAGAT

Product: p-cumate dioxygenase large subunit (CmtAb)

Products: NA

Alternate protein names: 2-chlorobenzoate 1,2-dioxygenase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MENESKLVEVDEKHLQFRVAREAFVSEQILSGEYEKIFDRCWLYVGHVSEFSKPGDFVTRTIARRNLLVTMGTDNRINAF
FNTCPHRGATVCRDRAGNSKNFQCFYHGWVFGCDGNLKSQPGKDRYCADFIASGAGNLVSVPRFDIYAGFCFVSFNAEVE
PLPDYLAGAKEYLDLVSRYSESGMAISSGTQEYAIRANWKLLVENSVDGYHAVSTHATYLDYLKNTNGSLSSTKLEGTSY
DLGNGHAVLEYTAPWGRPIASWVPLWGEQGKKEIDQIYARLVELHGEDMADRMAHKNRNLLIFPNLVVNDIMAITVRTFY
PQAPNYMLVNGWSLAPREESEWAKKFRLSNFLEFLGPGGFATPDDVEALESCQNGFSNYRLVPWSDISKGMGKASPNYDD
ELQMRAFWTRWNQYISGSPSPQATIRRLPSATAA

Sequences:

>Translated_434_residues
MENESKLVEVDEKHLQFRVAREAFVSEQILSGEYEKIFDRCWLYVGHVSEFSKPGDFVTRTIARRNLLVTMGTDNRINAF
FNTCPHRGATVCRDRAGNSKNFQCFYHGWVFGCDGNLKSQPGKDRYCADFIASGAGNLVSVPRFDIYAGFCFVSFNAEVE
PLPDYLAGAKEYLDLVSRYSESGMAISSGTQEYAIRANWKLLVENSVDGYHAVSTHATYLDYLKNTNGSLSSTKLEGTSY
DLGNGHAVLEYTAPWGRPIASWVPLWGEQGKKEIDQIYARLVELHGEDMADRMAHKNRNLLIFPNLVVNDIMAITVRTFY
PQAPNYMLVNGWSLAPREESEWAKKFRLSNFLEFLGPGGFATPDDVEALESCQNGFSNYRLVPWSDISKGMGKASPNYDD
ELQMRAFWTRWNQYISGSPSPQATIRRLPSATAA
>Mature_434_residues
MENESKLVEVDEKHLQFRVAREAFVSEQILSGEYEKIFDRCWLYVGHVSEFSKPGDFVTRTIARRNLLVTMGTDNRINAF
FNTCPHRGATVCRDRAGNSKNFQCFYHGWVFGCDGNLKSQPGKDRYCADFIASGAGNLVSVPRFDIYAGFCFVSFNAEVE
PLPDYLAGAKEYLDLVSRYSESGMAISSGTQEYAIRANWKLLVENSVDGYHAVSTHATYLDYLKNTNGSLSSTKLEGTSY
DLGNGHAVLEYTAPWGRPIASWVPLWGEQGKKEIDQIYARLVELHGEDMADRMAHKNRNLLIFPNLVVNDIMAITVRTFY
PQAPNYMLVNGWSLAPREESEWAKKFRLSNFLEFLGPGGFATPDDVEALESCQNGFSNYRLVPWSDISKGMGKASPNYDD
ELQMRAFWTRWNQYISGSPSPQATIRRLPSATAA

Specific function: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=388, Percent_Identity=28.6082474226804, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788103, Length=204, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.13 [H]

Molecular weight: Translated: 48916; Mature: 48916

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENESKLVEVDEKHLQFRVAREAFVSEQILSGEYEKIFDRCWLYVGHVSEFSKPGDFVTR
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TIARRNLLVTMGTDNRINAFFNTCPHRGATVCRDRAGNSKNFQCFYHGWVFGCDGNLKSQ
HHHHCCEEEEECCCCCHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEEEEECCCCCCCC
PGKDRYCADFIASGAGNLVSVPRFDIYAGFCFVSFNAEVEPLPDYLAGAKEYLDLVSRYS
CCCCHHHHHHHHCCCCCEEECCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
ESGMAISSGTQEYAIRANWKLLVENSVDGYHAVSTHATYLDYLKNTNGSLSSTKLEGTSY
HCCCEEECCCCEEEEEECEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEECCCCE
DLGNGHAVLEYTAPWGRPIASWVPLWGEQGKKEIDQIYARLVELHGEDMADRMAHKNRNL
ECCCCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCE
LIFPNLVVNDIMAITVRTFYPQAPNYMLVNGWSLAPREESEWAKKFRLSNFLEFLGPGGF
EEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ATPDDVEALESCQNGFSNYRLVPWSDISKGMGKASPNYDDELQMRAFWTRWNQYISGSPS
CCCHHHHHHHHHHCCHHCCEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
PQATIRRLPSATAA
HHHHHHHCCCCCCC
>Mature Secondary Structure
MENESKLVEVDEKHLQFRVAREAFVSEQILSGEYEKIFDRCWLYVGHVSEFSKPGDFVTR
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TIARRNLLVTMGTDNRINAFFNTCPHRGATVCRDRAGNSKNFQCFYHGWVFGCDGNLKSQ
HHHHCCEEEEECCCCCHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEEEEECCCCCCCC
PGKDRYCADFIASGAGNLVSVPRFDIYAGFCFVSFNAEVEPLPDYLAGAKEYLDLVSRYS
CCCCHHHHHHHHCCCCCEEECCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
ESGMAISSGTQEYAIRANWKLLVENSVDGYHAVSTHATYLDYLKNTNGSLSSTKLEGTSY
HCCCEEECCCCEEEEEECEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEECCCCE
DLGNGHAVLEYTAPWGRPIASWVPLWGEQGKKEIDQIYARLVELHGEDMADRMAHKNRNL
ECCCCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCE
LIFPNLVVNDIMAITVRTFYPQAPNYMLVNGWSLAPREESEWAKKFRLSNFLEFLGPGGF
EEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ATPDDVEALESCQNGFSNYRLVPWSDISKGMGKASPNYDDELQMRAFWTRWNQYISGSPS
CCCHHHHHHHHHHCCHHCCEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
PQATIRRLPSATAA
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7530709; 1370284 [H]