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The map label for this gene is thcD [H]

Identifier: 91782277

GI number: 91782277

Start: 1005213

End: 1006421

Strand: Direct

Name: thcD [H]

Synonym: Bxe_A3557

Alternate gene names: 91782277

Gene position: 1005213-1006421 (Clockwise)

Preceding gene: 91782276

Following gene: 91782278

Centisome position: 20.53

GC content: 62.86

Gene sequence:

>1209_bases
ATGGACGAAGGGATCAAAAGGATTGTGATCGCAGGCGCTGGCCAGGCCGGTGCCACAGTTGCGTTTGGCCTGCGACGCGG
CGGATTCGGTGGCGAGATCGCGCTGGTAGGCGATGAAGTGCATCTGCCCTATGAGCGACCCCAGTTGTCGAAGGAGATGC
TTCGGCCCGAGTCGGCTGGACACCGTTCGATCAGGACACGGGCGGAGTTCAATGAGCAACGCATCGAGCTCGTCCTCGGG
CGCAGGGTGGTCGCAGTCGATGTTGAACGCCATCAGATCCAGCTGGATGACGGGCGTGGGCTTGCTTTTGACCGGCTGGT
ACTTGCCGGCGGCGTGAGCCCCCGGCAGTTGCCCGGCGGACCTCATGATGCCGCCGCGGTCCGGTATCTGCGCACCGTCG
AAGACGCCAGCGGTCTGTGTGCCGACCTGGAGACCGGGGGGCACGTCGCCATTGTGGGCGGAGGCGTGATTGGGCTGGAA
GTCGCAGCGGCTGCACGTGCCAGGGGCTGCGAGGTCACCGTGATAGAGGCTGCAGATCGTCTGATGTCGCGTAGTGTCGA
TGACACGGTGTCCCGCTTCCTCGACCGGGCACATCGAGGCAAGGGTGTGGATATTCGCTACAGGGTGCTTGTCACCGAAC
TGCGCGACGGCAGATATCTTGAATTGTCCGATGGCAGTACGGTCGCGGCGGACACCGTCCTGGTGGGGATCGGCGTGACG
CCGAACATAGCGGGCTTCGACGCGCTGGGGATTACCGATGCCGCCGGAGTGCGTGTGGATGCATATGGCCAGACAGCGCT
ACAGGGCATCTTCGCTACAGGCGATATTGCCTCGCAGAAAAACGAAAACGGATTCGGGCGGATCGAGACCTGGGCGAATG
CTCAGGACCACGCCCTGAATGTTGCGAGGAACCTGCTGGGCGAGAGGGTTGCGTACGAGTCGCCGGTGTGGTTCTGGTCC
GATCAGGGGACGACCAATCTCCAGGTGGTGGGCAACGCGACACGGGGAAGAAGGATTGTCCGGGGCGATGAGCGCGGAGA
AGCCTTCAGTGTTTTCTGGCTGGGCGAAGGCGATCGAGTCACCGGATGCGCGTCGGTCAATATGCCAAAGGACATGGCGC
TCGCACGCCGCTGGGTAAAGCAGGGAAATCGGGTGGATCCGCATCAGCTCGCCGAGCCGGGCACGCCATTACGCAATTGT
GCGCTTTGA

Upstream 100 bases:

>100_bases
TGACATGAGTAAGGAGCAGCATAAAAATAAATAAACAGACGGGCTAGTCGGTTTGTATTTTTGATGACCGGGACGGGCAA
TTTAATCAACTGGAACGAGA

Downstream 100 bases:

>100_bases
AGGCACAGGTTCAGAACCACGAGGATAAGACCATGGAGAATGAGTCGAAGTTGGTGGAGGTAGACGAAAAGCACCTCCAG
TTCCGGGTGGCCCGGGAAGC

Product: p-cumate dioxygenase ferredoxin reductase subunit (CmtAa)

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MDEGIKRIVIAGAGQAGATVAFGLRRGGFGGEIALVGDEVHLPYERPQLSKEMLRPESAGHRSIRTRAEFNEQRIELVLG
RRVVAVDVERHQIQLDDGRGLAFDRLVLAGGVSPRQLPGGPHDAAAVRYLRTVEDASGLCADLETGGHVAIVGGGVIGLE
VAAAARARGCEVTVIEAADRLMSRSVDDTVSRFLDRAHRGKGVDIRYRVLVTELRDGRYLELSDGSTVAADTVLVGIGVT
PNIAGFDALGITDAAGVRVDAYGQTALQGIFATGDIASQKNENGFGRIETWANAQDHALNVARNLLGERVAYESPVWFWS
DQGTTNLQVVGNATRGRRIVRGDERGEAFSVFWLGEGDRVTGCASVNMPKDMALARRWVKQGNRVDPHQLAEPGTPLRNC
AL

Sequences:

>Translated_402_residues
MDEGIKRIVIAGAGQAGATVAFGLRRGGFGGEIALVGDEVHLPYERPQLSKEMLRPESAGHRSIRTRAEFNEQRIELVLG
RRVVAVDVERHQIQLDDGRGLAFDRLVLAGGVSPRQLPGGPHDAAAVRYLRTVEDASGLCADLETGGHVAIVGGGVIGLE
VAAAARARGCEVTVIEAADRLMSRSVDDTVSRFLDRAHRGKGVDIRYRVLVTELRDGRYLELSDGSTVAADTVLVGIGVT
PNIAGFDALGITDAAGVRVDAYGQTALQGIFATGDIASQKNENGFGRIETWANAQDHALNVARNLLGERVAYESPVWFWS
DQGTTNLQVVGNATRGRRIVRGDERGEAFSVFWLGEGDRVTGCASVNMPKDMALARRWVKQGNRVDPHQLAEPGTPLRNC
AL
>Mature_402_residues
MDEGIKRIVIAGAGQAGATVAFGLRRGGFGGEIALVGDEVHLPYERPQLSKEMLRPESAGHRSIRTRAEFNEQRIELVLG
RRVVAVDVERHQIQLDDGRGLAFDRLVLAGGVSPRQLPGGPHDAAAVRYLRTVEDASGLCADLETGGHVAIVGGGVIGLE
VAAAARARGCEVTVIEAADRLMSRSVDDTVSRFLDRAHRGKGVDIRYRVLVTELRDGRYLELSDGSTVAADTVLVGIGVT
PNIAGFDALGITDAAGVRVDAYGQTALQGIFATGDIASQKNENGFGRIETWANAQDHALNVARNLLGERVAYESPVWFWS
DQGTTNLQVVGNATRGRRIVRGDERGEAFSVFWLGEGDRVTGCASVNMPKDMALARRWVKQGNRVDPHQLAEPGTPLRNC
AL

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=372, Percent_Identity=30.9139784946237, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI21389617, Length=372, Percent_Identity=30.9139784946237, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI65787454, Length=372, Percent_Identity=30.9139784946237, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=26.1127596439169, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=26.1127596439169, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1788892, Length=397, Percent_Identity=34.0050377833753, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786307, Length=188, Percent_Identity=29.7872340425532, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789065, Length=316, Percent_Identity=26.5822784810127, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=25.7042253521127, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=328, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585130, Length=401, Percent_Identity=22.1945137157107, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24639257, Length=401, Percent_Identity=23.1920199501247, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281359715, Length=401, Percent_Identity=23.1920199501247, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281359713, Length=401, Percent_Identity=23.1920199501247, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24639250, Length=401, Percent_Identity=23.1920199501247, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI18543267, Length=401, Percent_Identity=23.1920199501247, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24639252, Length=401, Percent_Identity=23.1920199501247, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24581020, Length=359, Percent_Identity=25.0696378830084, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28573993, Length=359, Percent_Identity=25.0696378830084, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43132; Mature: 43132

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEGIKRIVIAGAGQAGATVAFGLRRGGFGGEIALVGDEVHLPYERPQLSKEMLRPESAG
CCCCCEEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCEECCCCCCHHHHHHHCCCCCC
HRSIRTRAEFNEQRIELVLGRRVVAVDVERHQIQLDDGRGLAFDRLVLAGGVSPRQLPGG
CCHHHHHHHHCHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCHHHEEEECCCCCCCCCCC
PHDAAAVRYLRTVEDASGLCADLETGGHVAIVGGGVIGLEVAAAARARGCEVTVIEAADR
CCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCEEEHHHHHHHHCCCCEEEEEHHHHH
LMSRSVDDTVSRFLDRAHRGKGVDIRYRVLVTELRDGRYLELSDGSTVAADTVLVGIGVT
HHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEECCCCEEEEEEEEEEECCC
PNIAGFDALGITDAAGVRVDAYGQTALQGIFATGDIASQKNENGFGRIETWANAQDHALN
CCCCCCCCCCCCCCCCEEEECCCCHHHHHEEECCCCCCCCCCCCCCEEEECCCCHHHHHH
VARNLLGERVAYESPVWFWSDQGTTNLQVVGNATRGRRIVRGDERGEAFSVFWLGEGDRV
HHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCEE
TGCASVNMPKDMALARRWVKQGNRVDPHQLAEPGTPLRNCAL
EEEEECCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHCCC
>Mature Secondary Structure
MDEGIKRIVIAGAGQAGATVAFGLRRGGFGGEIALVGDEVHLPYERPQLSKEMLRPESAG
CCCCCEEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCEECCCCCCHHHHHHHCCCCCC
HRSIRTRAEFNEQRIELVLGRRVVAVDVERHQIQLDDGRGLAFDRLVLAGGVSPRQLPGG
CCHHHHHHHHCHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCHHHEEEECCCCCCCCCCC
PHDAAAVRYLRTVEDASGLCADLETGGHVAIVGGGVIGLEVAAAARARGCEVTVIEAADR
CCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCEEEHHHHHHHHCCCCEEEEEHHHHH
LMSRSVDDTVSRFLDRAHRGKGVDIRYRVLVTELRDGRYLELSDGSTVAADTVLVGIGVT
HHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEECCCCEEEEEEEEEEECCC
PNIAGFDALGITDAAGVRVDAYGQTALQGIFATGDIASQKNENGFGRIETWANAQDHALN
CCCCCCCCCCCCCCCCEEEECCCCHHHHHEEECCCCCCCCCCCCCCEEEECCCCHHHHHH
VARNLLGERVAYESPVWFWSDQGTTNLQVVGNATRGRRIVRGDERGEAFSVFWLGEGDRV
HHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCEE
TGCASVNMPKDMALARRWVKQGNRVDPHQLAEPGTPLRNCAL
EEEEECCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]