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The map label for this gene is emrB [H]

Identifier: 91782255

GI number: 91782255

Start: 978039

End: 979583

Strand: Reverse

Name: emrB [H]

Synonym: Bxe_A3580

Alternate gene names: 91782255

Gene position: 979583-978039 (Counterclockwise)

Preceding gene: 91782256

Following gene: 91782254

Centisome position: 20.01

GC content: 57.35

Gene sequence:

>1545_bases
ATGCCTGTTTCGAACGCCTCTTACACAGTCATCCCTGCCCGAACGCGTGCATTGATCACCATCTCGGTCATGCTCGCCAC
CATCATGCAGGTGCTGGACAGCACGATTGCCAACGTGGCGCTTCCCCATATGGAAGGCAGTCTGTCGGCCACCCAGGATC
AGATCACCTGGGTCCTGACGTCGTATATCGTGGCTGCAGCAATCGGCACACCACTCACCGGCTGGCTGGCAGCGCGCTTT
GGACGCAAGCGTCTTTTTCTGTGGTCGGTCGTCGGCTTCACCCTTGCATCCGTCGCTTGCGGAATGTCGACGTCACTGGC
GGAAATGGTCGCAGCGCGACTGCTTCAGGGCATGTTCGGCGCATCGCTGGTACCGCTCTCGCAGGCAGTCATGCTGGACA
TCAATCCGCCTGAAAAGCATGGACCGGCGATGGCCCTCTGGGGCGCTGGCGTCACCGTTGGACCGATCCTCGGCCCCACG
CTCGGCGGCTGGTTGACGGATAACTACAACTGGCGGTGGGTGTTCTATGTGAACGTGCCGGTCGGTATCGTTGCTCTTCT
CGGCATCCTGCTTTTCATGATGGAGACGAAAGTAAACTCACGCAAACTCGACCTGTTCGGATTTGCTACCTTGAGCATTG
CCATCGGTGCCCTGCAACTTTTCCTGGACCGCGGTGAAGTACAGGACTGGTTCGATTCGATCGAAATCTGGATCGAGTTG
CTAACTGCACTGATCGCGTTCGCCTTCTTTCTTGTGCATACGTTAACGGTTGAAACCACGTCGTTCTTCAATCGCGAACT
GGCGCGCAATCGCAACTTTGTGATCGGTTGCGGATTCTATTTTGTGATGGGCGCGGTGCTATACGCGACGCGCGCCTTGC
TGCCGCCGCTTCTGCAAAATCTCATGCGGTACTCCGTGGTCCAAACGGGACTTGCTACCGCGCCCAGTGGCGCCGGCACA
ATGATCGCCATGATGATGGCTGGCCGGCTTGTCGGTCCGCTCAAGCCACGTAACCTGATCGCCATCGGCTTTTCGATCGC
CGCCTTCTCGCTGTGGCAGATGTCGCACTACAACCTGTACATGGCGGAGACCACGATTATCTGGTCGGGTTTCCTCCAGG
GCCTTGGGCTCGGCTTCATTTCGGTTCCGCTCACAACCGCGACCTTCTCCACTCTTGCCGCTCAACTGCGCGGCGACGGC
ACATCCATCTATAGTCTGAGCCGCAATATTGGCAGCAGTATCGGTATTTCCTTTGTACAGACTGAACTCACGCGTAACAC
GCAGGTAGCGCATTCATCGCTGGTCGAACACATCAACGTCTATAACCCGCTCGCGCAGCCAGATACCTTGCCATCCACGT
GGGACATCCATTCGTCCGCTGGACTGGCCTCACTGAATGTCGAAGTCACCCGGCAGGCATCGATGATTGCATATGTGGAT
GACTTCAGACTCCTGTTCCTGCTGGCGCTTGCCGTTATGCCATTCCTGTTGCTGTTAGAGACGCCTCGAGCACGGCGTCA
AATCCCCGCCGTGCCAGAACATTGA

Upstream 100 bases:

>100_bases
GAAAAGTGTGCAAATCGGTCGTACGCGTATGAATTAGCAGCGGCGACGGGCAGCGCGCTGACAGTGTGCCAATACCTGCA
CAGAATACGTGCCGCCACTC

Downstream 100 bases:

>100_bases
CCTCCGCGTGGGCGGCGGCTGAGACAGCACCGCATGAACTACATCGGTACTGGCAGTTTGCACTTTATATTGATAGTTGG
CTAACGAATGTTTAAAGGAA

Product: major facilitator superfamily multidrug efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MPVSNASYTVIPARTRALITISVMLATIMQVLDSTIANVALPHMEGSLSATQDQITWVLTSYIVAAAIGTPLTGWLAARF
GRKRLFLWSVVGFTLASVACGMSTSLAEMVAARLLQGMFGASLVPLSQAVMLDINPPEKHGPAMALWGAGVTVGPILGPT
LGGWLTDNYNWRWVFYVNVPVGIVALLGILLFMMETKVNSRKLDLFGFATLSIAIGALQLFLDRGEVQDWFDSIEIWIEL
LTALIAFAFFLVHTLTVETTSFFNRELARNRNFVIGCGFYFVMGAVLYATRALLPPLLQNLMRYSVVQTGLATAPSGAGT
MIAMMMAGRLVGPLKPRNLIAIGFSIAAFSLWQMSHYNLYMAETTIIWSGFLQGLGLGFISVPLTTATFSTLAAQLRGDG
TSIYSLSRNIGSSIGISFVQTELTRNTQVAHSSLVEHINVYNPLAQPDTLPSTWDIHSSAGLASLNVEVTRQASMIAYVD
DFRLLFLLALAVMPFLLLLETPRARRQIPAVPEH

Sequences:

>Translated_514_residues
MPVSNASYTVIPARTRALITISVMLATIMQVLDSTIANVALPHMEGSLSATQDQITWVLTSYIVAAAIGTPLTGWLAARF
GRKRLFLWSVVGFTLASVACGMSTSLAEMVAARLLQGMFGASLVPLSQAVMLDINPPEKHGPAMALWGAGVTVGPILGPT
LGGWLTDNYNWRWVFYVNVPVGIVALLGILLFMMETKVNSRKLDLFGFATLSIAIGALQLFLDRGEVQDWFDSIEIWIEL
LTALIAFAFFLVHTLTVETTSFFNRELARNRNFVIGCGFYFVMGAVLYATRALLPPLLQNLMRYSVVQTGLATAPSGAGT
MIAMMMAGRLVGPLKPRNLIAIGFSIAAFSLWQMSHYNLYMAETTIIWSGFLQGLGLGFISVPLTTATFSTLAAQLRGDG
TSIYSLSRNIGSSIGISFVQTELTRNTQVAHSSLVEHINVYNPLAQPDTLPSTWDIHSSAGLASLNVEVTRQASMIAYVD
DFRLLFLLALAVMPFLLLLETPRARRQIPAVPEH
>Mature_513_residues
PVSNASYTVIPARTRALITISVMLATIMQVLDSTIANVALPHMEGSLSATQDQITWVLTSYIVAAAIGTPLTGWLAARFG
RKRLFLWSVVGFTLASVACGMSTSLAEMVAARLLQGMFGASLVPLSQAVMLDINPPEKHGPAMALWGAGVTVGPILGPTL
GGWLTDNYNWRWVFYVNVPVGIVALLGILLFMMETKVNSRKLDLFGFATLSIAIGALQLFLDRGEVQDWFDSIEIWIELL
TALIAFAFFLVHTLTVETTSFFNRELARNRNFVIGCGFYFVMGAVLYATRALLPPLLQNLMRYSVVQTGLATAPSGAGTM
IAMMMAGRLVGPLKPRNLIAIGFSIAAFSLWQMSHYNLYMAETTIIWSGFLQGLGLGFISVPLTTATFSTLAAQLRGDGT
SIYSLSRNIGSSIGISFVQTELTRNTQVAHSSLVEHINVYNPLAQPDTLPSTWDIHSSAGLASLNVEVTRQASMIAYVDD
FRLLFLLALAVMPFLLLLETPRARRQIPAVPEH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=432, Percent_Identity=40.9722222222222, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1788710, Length=507, Percent_Identity=35.3057199211045, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1790195, Length=433, Percent_Identity=29.0993071593533, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1788392, Length=413, Percent_Identity=30.2663438256659, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=28.9351851851852, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87082320, Length=184, Percent_Identity=22.2826086956522, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790146, Length=159, Percent_Identity=25.1572327044025, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=27.0676691729323, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=134, Percent_Identity=28.3582089552239, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319480, Length=190, Percent_Identity=22.6315789473684, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=130, Percent_Identity=29.2307692307692, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323000, Length=122, Percent_Identity=31.9672131147541, Blast_Score=63, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=230, Percent_Identity=23.4782608695652, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55906; Mature: 55775

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSNASYTVIPARTRALITISVMLATIMQVLDSTIANVALPHMEGSLSATQDQITWVLT
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SYIVAAAIGTPLTGWLAARFGRKRLFLWSVVGFTLASVACGMSTSLAEMVAARLLQGMFG
HHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
ASLVPLSQAVMLDINPPEKHGPAMALWGAGVTVGPILGPTLGGWLTDNYNWRWVFYVNVP
CCHHCCCCEEEEECCCCCCCCCEEEEECCCCEECCHHCCCCCCCCCCCCCEEEEEEEECC
VGIVALLGILLFMMETKVNSRKLDLFGFATLSIAIGALQLFLDRGEVQDWFDSIEIWIEL
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LTALIAFAFFLVHTLTVETTSFFNRELARNRNFVIGCGFYFVMGAVLYATRALLPPLLQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
LMRYSVVQTGLATAPSGAGTMIAMMMAGRLVGPLKPRNLIAIGFSIAAFSLWQMSHYNLY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCEEE
MAETTIIWSGFLQGLGLGFISVPLTTATFSTLAAQLRGDGTSIYSLSRNIGSSIGISFVQ
EEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCHHHHH
TELTRNTQVAHSSLVEHINVYNPLAQPDTLPSTWDIHSSAGLASLNVEVTRQASMIAYVD
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHH
DFRLLFLLALAVMPFLLLLETPRARRQIPAVPEH
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure 
PVSNASYTVIPARTRALITISVMLATIMQVLDSTIANVALPHMEGSLSATQDQITWVLT
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SYIVAAAIGTPLTGWLAARFGRKRLFLWSVVGFTLASVACGMSTSLAEMVAARLLQGMFG
HHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
ASLVPLSQAVMLDINPPEKHGPAMALWGAGVTVGPILGPTLGGWLTDNYNWRWVFYVNVP
CCHHCCCCEEEEECCCCCCCCCEEEEECCCCEECCHHCCCCCCCCCCCCCEEEEEEEECC
VGIVALLGILLFMMETKVNSRKLDLFGFATLSIAIGALQLFLDRGEVQDWFDSIEIWIEL
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LTALIAFAFFLVHTLTVETTSFFNRELARNRNFVIGCGFYFVMGAVLYATRALLPPLLQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
LMRYSVVQTGLATAPSGAGTMIAMMMAGRLVGPLKPRNLIAIGFSIAAFSLWQMSHYNLY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCEEE
MAETTIIWSGFLQGLGLGFISVPLTTATFSTLAAQLRGDGTSIYSLSRNIGSSIGISFVQ
EEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCHHHHH
TELTRNTQVAHSSLVEHINVYNPLAQPDTLPSTWDIHSSAGLASLNVEVTRQASMIAYVD
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHH
DFRLLFLLALAVMPFLLLLETPRARRQIPAVPEH
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]