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The map label for this gene is alkJ [H]

Identifier: 91782240

GI number: 91782240

Start: 960408

End: 962057

Strand: Reverse

Name: alkJ [H]

Synonym: Bxe_A3595

Alternate gene names: 91782240

Gene position: 962057-960408 (Counterclockwise)

Preceding gene: 91782241

Following gene: 91782222

Centisome position: 19.65

GC content: 59.76

Gene sequence:

>1650_bases
GTGGAAAAGATCGAGACCGAATTTGATTACATCATCGTCGGCGCGGGCTCGGCAGGATGTGTGCTCGCTAACCGGCTCTC
GGCGGATCCGTCTGTCAAGGTTGCCTTGATCGAGGCGGGTCCGTCGGACCGCAGGTTCCCGACGAACATCAAATCGTCGA
TGCCTGCCGGTATGCTGTTCCTGTTGCCACATTCGAAATACAACTGGCAGTACACGTTCACGGGCGGAAGCGGTGTCAAC
GGGCGGTCGCTGCTGTGTCCGAGAGGCAAACTGATGGGCGGCACCAGTTCGGTCAACGGCATGGTCTACATTCGCGGTCA
CCGGCTCGACTACGACGACTGGGCAGCGCTCGGCAACGACGGCTGGTCATATCAGGAAGTGCTGCCCTTCTTCAAGAAGC
ACGAAAACAACACACAGGGCGAAGCGCCGTTTCATGGCGTGGGCGGCGAAGTGGAAGTGAGTGTGCCGGAGAATCCAAAC
ATTCTTTCCCGCACATTCATTGAAGCAGCGCGTGAAGTAGGTCTGCCGATGAACGCGGATGCTAACGGCACCAGTCAGGA
CGGCATCGGCTTCAATCACGTGAATCACAAATATGGCAGGCGTTACAGCAGCTCGCGTGCGTTTCTGCACCCAATCCTGC
ATCGGCGCAACCTGCACGTGCTGACAGATACGCTCGTCGAACGAATTCTCTTTTCCGGCGACCGGGCAACAGGCATTTCT
ATCCTTCAGGGTGCAGCCCCAACGACGCTTAATGCCACCCGTGAAGTGATCCTGAGCGGCGGCGCAATCAACTCACCGCA
ACTGCTGATGCTGTCGGGAATCGGCCCGCACGCCGAACTGGCACGCCTTGGCATCGAAACGCGAGTCGATTTGCCGGGCG
TGGGCGAGAATCTGCAGGATCACCCGACCGTCCAGGTGTCACGAAGCAACCCCTCGGCCGAATCTTATGCGCTGACGCTG
CGTGCGTGGCCGCGCGTGCTGGGCACGCCATTCGCGTATCTGTTCGCAAAGAAAGGCATGTTGGCCACGCACGGCGCGGA
GGCCGGCGGTTTCGTGCGCACGCTTCCCGAACTCGACAGGCCGGACATCCAGCTCACCTTCGTCGCGACGATCAAGAAGT
CGGTGTACAAGATGCCGCGCACGCACGGCATGATGTTGATGGTGCACCTGATGCGCCCGCGTACGCGCGGCCGCATCAGG
TTGACCTCGTCCAGCATCCAAGACAAACCCGAGTTGCATCCTCGCTTTCTCGACGATCCCGAAGACTTGCAAACGCTTTT
GCGCGGCGTGCATCAGGCGAGGCGTATTCTCGGCACGAAAGCGTTTGCGCCCTATGTCGGCGAAGAAGTGACGCCTGGCG
CGCAATATATGAGCGACGAGGATCTCATCAAGGCGATCCGCGCACAGGTCGGCACCGCATATCACCCGGTGGGAACGTGC
AAGATGGGCCCCGCATCGGATCTGATGGCGGTAGTCGACAACGAATTGCGTGTGCGAGGCGTGCGAGGTTTACGCGTGGT
GGATGCATCCATCATGCCGAACATCGTGGGCGGCAACACGAATGCACCTGCCATGATGATTGGCGAGCGTGCAGCATCGT
TCATTCTGGGTGAGAAGGTAAGCCGGCCGGAAGCGGTAGAGCTCGCCTGA

Upstream 100 bases:

>100_bases
AGCCCGCTGTCCGTTCCTGAACGCGGCGTTGTAATCCGCGCGAAGCGGCGCGGGATACATCGCGCCGCCAGAACAACTGC
AGTATCGAGGAGACACGATC

Downstream 100 bases:

>100_bases
CGGCACGGACCGGAAGATTTGAATAGCGAGCGCTTCGGCCAAGCGCTCGCTATGTGGGAACAGATCAGGCCGCAATATTG
GCCCTCTTCTTGAGCCGTTC

Product: putative glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MEKIETEFDYIIVGAGSAGCVLANRLSADPSVKVALIEAGPSDRRFPTNIKSSMPAGMLFLLPHSKYNWQYTFTGGSGVN
GRSLLCPRGKLMGGTSSVNGMVYIRGHRLDYDDWAALGNDGWSYQEVLPFFKKHENNTQGEAPFHGVGGEVEVSVPENPN
ILSRTFIEAAREVGLPMNADANGTSQDGIGFNHVNHKYGRRYSSSRAFLHPILHRRNLHVLTDTLVERILFSGDRATGIS
ILQGAAPTTLNATREVILSGGAINSPQLLMLSGIGPHAELARLGIETRVDLPGVGENLQDHPTVQVSRSNPSAESYALTL
RAWPRVLGTPFAYLFAKKGMLATHGAEAGGFVRTLPELDRPDIQLTFVATIKKSVYKMPRTHGMMLMVHLMRPRTRGRIR
LTSSSIQDKPELHPRFLDDPEDLQTLLRGVHQARRILGTKAFAPYVGEEVTPGAQYMSDEDLIKAIRAQVGTAYHPVGTC
KMGPASDLMAVVDNELRVRGVRGLRVVDASIMPNIVGGNTNAPAMMIGERAASFILGEKVSRPEAVELA

Sequences:

>Translated_549_residues
MEKIETEFDYIIVGAGSAGCVLANRLSADPSVKVALIEAGPSDRRFPTNIKSSMPAGMLFLLPHSKYNWQYTFTGGSGVN
GRSLLCPRGKLMGGTSSVNGMVYIRGHRLDYDDWAALGNDGWSYQEVLPFFKKHENNTQGEAPFHGVGGEVEVSVPENPN
ILSRTFIEAAREVGLPMNADANGTSQDGIGFNHVNHKYGRRYSSSRAFLHPILHRRNLHVLTDTLVERILFSGDRATGIS
ILQGAAPTTLNATREVILSGGAINSPQLLMLSGIGPHAELARLGIETRVDLPGVGENLQDHPTVQVSRSNPSAESYALTL
RAWPRVLGTPFAYLFAKKGMLATHGAEAGGFVRTLPELDRPDIQLTFVATIKKSVYKMPRTHGMMLMVHLMRPRTRGRIR
LTSSSIQDKPELHPRFLDDPEDLQTLLRGVHQARRILGTKAFAPYVGEEVTPGAQYMSDEDLIKAIRAQVGTAYHPVGTC
KMGPASDLMAVVDNELRVRGVRGLRVVDASIMPNIVGGNTNAPAMMIGERAASFILGEKVSRPEAVELA
>Mature_549_residues
MEKIETEFDYIIVGAGSAGCVLANRLSADPSVKVALIEAGPSDRRFPTNIKSSMPAGMLFLLPHSKYNWQYTFTGGSGVN
GRSLLCPRGKLMGGTSSVNGMVYIRGHRLDYDDWAALGNDGWSYQEVLPFFKKHENNTQGEAPFHGVGGEVEVSVPENPN
ILSRTFIEAAREVGLPMNADANGTSQDGIGFNHVNHKYGRRYSSSRAFLHPILHRRNLHVLTDTLVERILFSGDRATGIS
ILQGAAPTTLNATREVILSGGAINSPQLLMLSGIGPHAELARLGIETRVDLPGVGENLQDHPTVQVSRSNPSAESYALTL
RAWPRVLGTPFAYLFAKKGMLATHGAEAGGFVRTLPELDRPDIQLTFVATIKKSVYKMPRTHGMMLMVHLMRPRTRGRIR
LTSSSIQDKPELHPRFLDDPEDLQTLLRGVHQARRILGTKAFAPYVGEEVTPGAQYMSDEDLIKAIRAQVGTAYHPVGTC
KMGPASDLMAVVDNELRVRGVRGLRVVDASIMPNIVGGNTNAPAMMIGERAASFILGEKVSRPEAVELA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=544, Percent_Identity=41.7279411764706, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI1786503, Length=542, Percent_Identity=40.2214022140221, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17532301, Length=553, Percent_Identity=36.7088607594937, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24642042, Length=591, Percent_Identity=37.7326565143824, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24650267, Length=573, Percent_Identity=36.9982547993019, Blast_Score=322, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24642059, Length=578, Percent_Identity=37.5432525951557, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24642055, Length=582, Percent_Identity=35.9106529209622, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI45551458, Length=572, Percent_Identity=35.4895104895105, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI45549471, Length=572, Percent_Identity=35.4895104895105, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI17137792, Length=557, Percent_Identity=36.0861759425494, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI18859995, Length=572, Percent_Identity=36.5384615384615, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24642039, Length=571, Percent_Identity=36.9527145359019, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24642048, Length=576, Percent_Identity=35.5902777777778, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24642037, Length=580, Percent_Identity=35.8620689655172, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24642051, Length=570, Percent_Identity=34.7368421052632, Blast_Score=294, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24642035, Length=586, Percent_Identity=36.6894197952218, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI18859993, Length=581, Percent_Identity=34.4234079173838, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24642057, Length=614, Percent_Identity=31.4332247557003, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24645930, Length=592, Percent_Identity=28.2094594594595, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59776; Mature: 59776

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIETEFDYIIVGAGSAGCVLANRLSADPSVKVALIEAGPSDRRFPTNIKSSMPAGMLF
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCEEE
LLPHSKYNWQYTFTGGSGVNGRSLLCPRGKLMGGTSSVNGMVYIRGHRLDYDDWAALGND
EEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCC
GWSYQEVLPFFKKHENNTQGEAPFHGVGGEVEVSVPENPNILSRTFIEAAREVGLPMNAD
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCC
ANGTSQDGIGFNHVNHKYGRRYSSSRAFLHPILHRRNLHVLTDTLVERILFSGDRATGIS
CCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHH
ILQGAAPTTLNATREVILSGGAINSPQLLMLSGIGPHAELARLGIETRVDLPGVGENLQD
HCCCCCCCCHHCCHHHEEECCCCCCCCEEEEECCCCHHHHHHCCCCEECCCCCCCCCCCC
HPTVQVSRSNPSAESYALTLRAWPRVLGTPFAYLFAKKGMLATHGAEAGGFVRTLPELDR
CCCEEEECCCCCCCCEEEEEEHHHHHHCCCHHHHHHCCCCEEECCCCCCCHHHHCCCCCC
PDIQLTFVATIKKSVYKMPRTHGMMLMVHLMRPRTRGRIRLTSSSIQDKPELHPRFLDDP
CCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCH
EDLQTLLRGVHQARRILGTKAFAPYVGEEVTPGAQYMSDEDLIKAIRAQVGTAYHPVGTC
HHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCC
KMGPASDLMAVVDNELRVRGVRGLRVVDASIMPNIVGGNTNAPAMMIGERAASFILGEKV
CCCCHHHHHHHHCCCCEEECCCCEEEEEHHHCCEECCCCCCCCEEEEHHHHHHHHHHHCC
SRPEAVELA
CCCCCCCCC
>Mature Secondary Structure
MEKIETEFDYIIVGAGSAGCVLANRLSADPSVKVALIEAGPSDRRFPTNIKSSMPAGMLF
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCEEE
LLPHSKYNWQYTFTGGSGVNGRSLLCPRGKLMGGTSSVNGMVYIRGHRLDYDDWAALGND
EEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCC
GWSYQEVLPFFKKHENNTQGEAPFHGVGGEVEVSVPENPNILSRTFIEAAREVGLPMNAD
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCC
ANGTSQDGIGFNHVNHKYGRRYSSSRAFLHPILHRRNLHVLTDTLVERILFSGDRATGIS
CCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHH
ILQGAAPTTLNATREVILSGGAINSPQLLMLSGIGPHAELARLGIETRVDLPGVGENLQD
HCCCCCCCCHHCCHHHEEECCCCCCCCEEEEECCCCHHHHHHCCCCEECCCCCCCCCCCC
HPTVQVSRSNPSAESYALTLRAWPRVLGTPFAYLFAKKGMLATHGAEAGGFVRTLPELDR
CCCEEEECCCCCCCCEEEEEEHHHHHHCCCHHHHHHCCCCEEECCCCCCCHHHHCCCCCC
PDIQLTFVATIKKSVYKMPRTHGMMLMVHLMRPRTRGRIRLTSSSIQDKPELHPRFLDDP
CCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCH
EDLQTLLRGVHQARRILGTKAFAPYVGEEVTPGAQYMSDEDLIKAIRAQVGTAYHPVGTC
HHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCC
KMGPASDLMAVVDNELRVRGVRGLRVVDASIMPNIVGGNTNAPAMMIGERAASFILGEKV
CCCCHHHHHHHHCCCCEEECCCCEEEEEHHHCCEECCCCCCCCEEEEHHHHHHHHHHHCC
SRPEAVELA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]