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The map label for this gene is pcnB [H]

Identifier: 91781890

GI number: 91781890

Start: 572891

End: 574480

Strand: Reverse

Name: pcnB [H]

Synonym: Bxe_A3955

Alternate gene names: 91781890

Gene position: 574480-572891 (Counterclockwise)

Preceding gene: 91781891

Following gene: 91781889

Centisome position: 11.73

GC content: 64.97

Gene sequence:

>1590_bases
GTGATCAAAAAATTTATCCGCAAGCTATTCGGCCAGGACACGGCACCCGCTGACGACACCCTGCCCGCCGAAGCCGAAGC
AGACGAAACCGGCAACGCACCGGCGCGCACGCCGTCAGGCGCGAGCAAAGCGCGTCGCAAGCCGGCCCGCTCCAGCGCGC
CCGCGAAAACCGTGCGCGATCCGGACGTGCCGGTCATCATTCCGCACGACGTGCACGGCATCGACCAGGCGCTGATCTCG
AGGAACGCGATTCGCGTGACCGAGGGTCTGCAACAGGCGGGGCACCGCGCGTTCATCGTCGGCGGGGCGGTGCGCGATCT
GCTGCTCGGTATCAAGCCGAAAGACTTCGACGTCGCCACGGACGCCACGCCCGAACAGGTGCAGAAGCTGTTCCGCCGCG
CGCGCATCATCGGCCGGCGGTTTCAGATCGTGCATGTGCAGTTCGGCCAGGAGATCATCGAGACCTCCACGTTCCGCGCG
CTGGTCGATCCGCCCGCGGCCGACGCGCCTCCGGCGCGCCGCCTGAAGCGCGACGAGCTCGACCGCCGCACGCATGCGGT
GGACGCGAGCGGCCGCGTGCTGCGCGACAACGTGTGGGGCGAGCAGCACGAAGACGCCACGCGCCGCGACTTCACGATCA
ACGCGATGTATTACGATCCGGCGACGCAAACCGTGCTGGATTATCACAACGGCATGGCCGACATGCGCGCGCGCCTGCTG
CGCATGATCGGCGACCCGGCTACGCGCTATCGCGAAGATCCGGTGCGCATGCTGCGCGTGGTGCGTTTCGCGGCGAAGCT
CGACTTCGAGATCGAGGACAGCACCCGCGAGCCGATCGCCGGCATGGCCGATCTGATCAACAACGTACCCGCCGCGCGTC
TCTTCGACGAGATGTTGAAGCTGATGCTCTCGGGCCATGCGCTCGCGTGTCTGAAGCGTCTGCGCCAGGAAGGTCTGCAT
CATGGCCTGCTGCCCCTGCTCGACGTGGTGCTCGAACAGCCCATCGGCGAGAAATTCATCACGCTCGCGCTGAACAATAC
GGACGCCCGCGTGCGCGCCGGCAAGCCGGTGTCGCCGGGCTTCCTGTTCGCCACGCTGTTGTGGCACGACGTGCAGCAGC
GCTGGCAAAAATTTGAAGCGAACGGCGAATATCCGGTTCCCGCCCTGCATCGTGCGATGGACGAAGTCCTCGACATGCAA
ACGGAGAAACTCGCGATCCACAAGCGCTTCTCGTCGGACATGCGCGAGATCTGGGGTTTGCAGTTGCGCCTCGAGAAACG
CTCCGGAAGAAGTGCGGTGAAGCTGCTGGAACACCAAAGATTTAGAGCGGGGTATGATTTCCTCCTGTTGCGCTGCGAAT
CAGGCGAACTCGATGAGTCGGTCGGTGCGTGGTGGACGGAGTTCATAGAAGGAGACGTCGCCGCACGTGAGACATTGCTC
ACGCAAGGCGGGAAGGACCGAAGCCCCAGAAAACGACGGCGGCGCAGCAGTGGCGCCAGAAACCGCAAACCGGGCGACGG
ATTGGAGGGCGGCACGCCAGCCGAACGCACAGCCGACGAAGCAGGCCATGACGGCCCGCGCGAAGACTGA

Upstream 100 bases:

>100_bases
TCGAAAAAGTCACGGACCCGATCGCGACCAATCCCGACGACACATTGCGTGCCCATGCGCAGGCCAAAGGCTGGCGCATC
CTCGAACTCTTCCAACCCTC

Downstream 100 bases:

>100_bases
CACAGCGTTCGCTGAAGCCGTCGAACGACAGTTGCAGGAAGTTATGCCATGACGGTTGCTTATCTCGGCCTCGGCGCGAA
TCTCGGGGACGCGCGCCAGA

Product: Poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MIKKFIRKLFGQDTAPADDTLPAEAEADETGNAPARTPSGASKARRKPARSSAPAKTVRDPDVPVIIPHDVHGIDQALIS
RNAIRVTEGLQQAGHRAFIVGGAVRDLLLGIKPKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRA
LVDPPAADAPPARRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTINAMYYDPATQTVLDYHNGMADMRARLL
RMIGDPATRYREDPVRMLRVVRFAAKLDFEIEDSTREPIAGMADLINNVPAARLFDEMLKLMLSGHALACLKRLRQEGLH
HGLLPLLDVVLEQPIGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDVQQRWQKFEANGEYPVPALHRAMDEVLDMQ
TEKLAIHKRFSSDMREIWGLQLRLEKRSGRSAVKLLEHQRFRAGYDFLLLRCESGELDESVGAWWTEFIEGDVAARETLL
TQGGKDRSPRKRRRRSSGARNRKPGDGLEGGTPAERTADEAGHDGPRED

Sequences:

>Translated_529_residues
MIKKFIRKLFGQDTAPADDTLPAEAEADETGNAPARTPSGASKARRKPARSSAPAKTVRDPDVPVIIPHDVHGIDQALIS
RNAIRVTEGLQQAGHRAFIVGGAVRDLLLGIKPKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRA
LVDPPAADAPPARRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTINAMYYDPATQTVLDYHNGMADMRARLL
RMIGDPATRYREDPVRMLRVVRFAAKLDFEIEDSTREPIAGMADLINNVPAARLFDEMLKLMLSGHALACLKRLRQEGLH
HGLLPLLDVVLEQPIGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDVQQRWQKFEANGEYPVPALHRAMDEVLDMQ
TEKLAIHKRFSSDMREIWGLQLRLEKRSGRSAVKLLEHQRFRAGYDFLLLRCESGELDESVGAWWTEFIEGDVAARETLL
TQGGKDRSPRKRRRRSSGARNRKPGDGLEGGTPAERTADEAGHDGPRED
>Mature_529_residues
MIKKFIRKLFGQDTAPADDTLPAEAEADETGNAPARTPSGASKARRKPARSSAPAKTVRDPDVPVIIPHDVHGIDQALIS
RNAIRVTEGLQQAGHRAFIVGGAVRDLLLGIKPKDFDVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRA
LVDPPAADAPPARRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTINAMYYDPATQTVLDYHNGMADMRARLL
RMIGDPATRYREDPVRMLRVVRFAAKLDFEIEDSTREPIAGMADLINNVPAARLFDEMLKLMLSGHALACLKRLRQEGLH
HGLLPLLDVVLEQPIGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDVQQRWQKFEANGEYPVPALHRAMDEVLDMQ
TEKLAIHKRFSSDMREIWGLQLRLEKRSGRSAVKLLEHQRFRAGYDFLLLRCESGELDESVGAWWTEFIEGDVAARETLL
TQGGKDRSPRKRRRRSSGARNRKPGDGLEGGTPAERTADEAGHDGPRED

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=242, Percent_Identity=30.5785123966942, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI87081691, Length=437, Percent_Identity=45.766590389016, Blast_Score=347, Evalue=7e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 59449; Mature: 59449

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKFIRKLFGQDTAPADDTLPAEAEADETGNAPARTPSGASKARRKPARSSAPAKTVRD
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
PDVPVIIPHDVHGIDQALISRNAIRVTEGLQQAGHRAFIVGGAVRDLLLGIKPKDFDVAT
CCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCCCCC
DATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRALVDPPAADAPPARRLKRDEL
CCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DRRTHAVDASGRVLRDNVWGEQHEDATRRDFTINAMYYDPATQTVLDYHNGMADMRARLL
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHCCHHHHHHHHH
RMIGDPATRYREDPVRMLRVVRFAAKLDFEIEDSTREPIAGMADLINNVPAARLFDEMLK
HHHCCHHHHHHCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHH
LMLSGHALACLKRLRQEGLHHGLLPLLDVVLEQPIGEKFITLALNNTDARVRAGKPVSPG
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCH
FLFATLLWHDVQQRWQKFEANGEYPVPALHRAMDEVLDMQTEKLAIHKRFSSDMREIWGL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
QLRLEKRSGRSAVKLLEHQRFRAGYDFLLLRCESGELDESVGAWWTEFIEGDVAARETLL
HEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHH
TQGGKDRSPRKRRRRSSGARNRKPGDGLEGGTPAERTADEAGHDGPRED
HCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKKFIRKLFGQDTAPADDTLPAEAEADETGNAPARTPSGASKARRKPARSSAPAKTVRD
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
PDVPVIIPHDVHGIDQALISRNAIRVTEGLQQAGHRAFIVGGAVRDLLLGIKPKDFDVAT
CCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCCCCCC
DATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRALVDPPAADAPPARRLKRDEL
CCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DRRTHAVDASGRVLRDNVWGEQHEDATRRDFTINAMYYDPATQTVLDYHNGMADMRARLL
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHCCHHHHHHHHH
RMIGDPATRYREDPVRMLRVVRFAAKLDFEIEDSTREPIAGMADLINNVPAARLFDEMLK
HHHCCHHHHHHCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHH
LMLSGHALACLKRLRQEGLHHGLLPLLDVVLEQPIGEKFITLALNNTDARVRAGKPVSPG
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCH
FLFATLLWHDVQQRWQKFEANGEYPVPALHRAMDEVLDMQTEKLAIHKRFSSDMREIWGL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
QLRLEKRSGRSAVKLLEHQRFRAGYDFLLLRCESGELDESVGAWWTEFIEGDVAARETLL
HEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHH
TQGGKDRSPRKRRRRSSGARNRKPGDGLEGGTPAERTADEAGHDGPRED
HCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]