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The map label for this gene is ksgA

Identifier: 91781824

GI number: 91781824

Start: 494287

End: 495120

Strand: Direct

Name: ksgA

Synonym: Bxe_A4021

Alternate gene names: 91781824

Gene position: 494287-495120 (Clockwise)

Preceding gene: 91781823

Following gene: 91781826

Centisome position: 10.1

GC content: 63.67

Gene sequence:

>834_bases
ATGTCCACCAGCAGACAGCAACAGGGCCGGCACCAAGGTCATATCGCGCGCAAGCGTTTCGGTCAGAATTTTCTGGTCGA
CATGGGCGTGATCGATTCGATCGTCGACGTCATCCGGCCGCAGCGCGGCGAGCGCATGGTCGAGATCGGGCCGGGTCTCG
GCGCGCTCACCGAGCCGCTGATCGAGCGTCTGGCGACGCCGGAAGCGCCGTTGCACGCCGTCGAACTGGATCGTGATCTG
ATCGGCCGTCTGAAGACGAAATTCGGCGACCTGCTCGAACTGCACGCGGGTGACGCGCTCGCGTTCGACTTCGGCTCGCT
GGCCGCGCCCGGCGACAAGGCGTCGCTGCGCATTGTCGGCAATCTGCCGTACAACATTTCGAGCCCCTTGCTGTTTCATC
TCACGGCGTTCGCGCATTGCGTGATCGATCAGCACTTCATGCTGCAGAACGAAGTGGTCGAGCGGATGGTGGCGGAGCCG
GGCACCAAGGCGTTCAGCCGTCTCTCGGTGATGCTGCAATACCGCTACGTGATCGACAAGCAACTCGACGTGCCGCCCGA
AGCGTTCAACCCGCCGCCGAAGGTCGATTCGGCGATCGTGCGGATGATCCCGTACGAACTGCATGAACTGCCGCCCGTCG
ACGAGCGCGTGCTCGGTGAACTGGTGACGGCCGCGTTCTCGCAGCGCCGCAAGATGCTGCGCAACACGCTTGCCGCGTAT
CGCGATTCGGTGGATTTCGAAGGGCTGGGCTTCGACTTGCAGCGCCGTGCCGAAGACGTGCCGGTGGCGGAATACGTGCG
CGTCGCGCAGATCGTGGCAGCCAGCAAGGCTTAA

Upstream 100 bases:

>100_bases
CCGCGGGCAGCAGGTGCGGCACGGCGCGCCCGCGCAACCAAGCGCATCGAGCAGCAGGTTCTTTTTTTCCCAAGCAGTTC
TCTTTCTTAGCGTTTCTTCG

Downstream 100 bases:

>100_bases
GCGGTGGGCGTGGACGGTTGAGCTGCGAACTGGCGGCTTCGACGCGCTTGCGCTCGAAGTGGCCAGCCGTTGTTGCGGTG
CCCGAATTCATCACCCGCGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSTSRQQQGRHQGHIARKRFGQNFLVDMGVIDSIVDVIRPQRGERMVEIGPGLGALTEPLIERLATPEAPLHAVELDRDL
IGRLKTKFGDLLELHAGDALAFDFGSLAAPGDKASLRIVGNLPYNISSPLLFHLTAFAHCVIDQHFMLQNEVVERMVAEP
GTKAFSRLSVMLQYRYVIDKQLDVPPEAFNPPPKVDSAIVRMIPYELHELPPVDERVLGELVTAAFSQRRKMLRNTLAAY
RDSVDFEGLGFDLQRRAEDVPVAEYVRVAQIVAASKA

Sequences:

>Translated_277_residues
MSTSRQQQGRHQGHIARKRFGQNFLVDMGVIDSIVDVIRPQRGERMVEIGPGLGALTEPLIERLATPEAPLHAVELDRDL
IGRLKTKFGDLLELHAGDALAFDFGSLAAPGDKASLRIVGNLPYNISSPLLFHLTAFAHCVIDQHFMLQNEVVERMVAEP
GTKAFSRLSVMLQYRYVIDKQLDVPPEAFNPPPKVDSAIVRMIPYELHELPPVDERVLGELVTAAFSQRRKMLRNTLAAY
RDSVDFEGLGFDLQRRAEDVPVAEYVRVAQIVAASKA
>Mature_276_residues
STSRQQQGRHQGHIARKRFGQNFLVDMGVIDSIVDVIRPQRGERMVEIGPGLGALTEPLIERLATPEAPLHAVELDRDLI
GRLKTKFGDLLELHAGDALAFDFGSLAAPGDKASLRIVGNLPYNISSPLLFHLTAFAHCVIDQHFMLQNEVVERMVAEPG
TKAFSRLSVMLQYRYVIDKQLDVPPEAFNPPPKVDSAIVRMIPYELHELPPVDERVLGELVTAAFSQRRKMLRNTLAAYR
DSVDFEGLGFDLQRRAEDVPVAEYVRVAQIVAASKA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=231, Percent_Identity=32.9004329004329, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI156415992, Length=245, Percent_Identity=30.2040816326531, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=52.8301886792453, Blast_Score=268, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25146882, Length=253, Percent_Identity=31.2252964426877, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25141369, Length=284, Percent_Identity=24.6478873239437, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=187, Percent_Identity=29.9465240641711, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21358017, Length=238, Percent_Identity=31.9327731092437, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357273, Length=286, Percent_Identity=27.972027972028, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BURXL (Q145L1)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_557030.1
- ProteinModelPortal:   Q145L1
- SMR:   Q145L1
- GeneID:   4003386
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A4021
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30858; Mature: 30727

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 23-23 BINDING 25-25 BINDING 50-50 BINDING 75-75 BINDING 98-98 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSRQQQGRHQGHIARKRFGQNFLVDMGVIDSIVDVIRPQRGERMVEIGPGLGALTEPL
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
IERLATPEAPLHAVELDRDLIGRLKTKFGDLLELHAGDALAFDFGSLAAPGDKASLRIVG
HHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEE
NLPYNISSPLLFHLTAFAHCVIDQHFMLQNEVVERMVAEPGTKAFSRLSVMLQYRYVIDK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
QLDVPPEAFNPPPKVDSAIVRMIPYELHELPPVDERVLGELVTAAFSQRRKMLRNTLAAY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSVDFEGLGFDLQRRAEDVPVAEYVRVAQIVAASKA
HCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSRQQQGRHQGHIARKRFGQNFLVDMGVIDSIVDVIRPQRGERMVEIGPGLGALTEPL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
IERLATPEAPLHAVELDRDLIGRLKTKFGDLLELHAGDALAFDFGSLAAPGDKASLRIVG
HHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEE
NLPYNISSPLLFHLTAFAHCVIDQHFMLQNEVVERMVAEPGTKAFSRLSVMLQYRYVIDK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
QLDVPPEAFNPPPKVDSAIVRMIPYELHELPPVDERVLGELVTAAFSQRRKMLRNTLAAY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDSVDFEGLGFDLQRRAEDVPVAEYVRVAQIVAASKA
HCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA