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The map label for this gene is ybhO [H]

Identifier: 91781804

GI number: 91781804

Start: 466558

End: 467817

Strand: Direct

Name: ybhO [H]

Synonym: Bxe_A4041

Alternate gene names: 91781804

Gene position: 466558-467817 (Clockwise)

Preceding gene: 91781803

Following gene: 91781805

Centisome position: 9.53

GC content: 65.79

Gene sequence:

>1260_bases
GTGAGCGTCGGCGGCGCGCGCCGTTTCGCCCGACTGCGGCAATCGCTGCTCGGCCGCCGCTATTGGCAACGCTATCGGTT
CACCAGCGGCAACGACGTCAAGCTGCTGCGTTCCGGCGACGAGTTCTTCAGCGCATTGATCGCGCGGGTCGATGCCGCGC
AGAGCGACGCGGTGCTCGAAACGTATATCTTCTGCCATGACAACGCCGGTCAGGCGGTCAGCGCGGCGCTGCTGCGCGCC
GCCGCGCGCGGCGTCAAAGTGCGGGCCATCACCGACGGCATCGGCACCGCGCGTTTGCCGATGTTCAACGAGTGGGCCGC
CGCCGGCATCGACCATCGTATCTACAATCCGCACCTGTTCGGCCGCTTCGGCTTTTCGCGCACGCACCGCAAACTGGCGG
TGATCGACGATCAGTTCGCGTTCTGCGGCGGCATCAACATCGTCGACGATTACGAGAACAACGGCGAGAAGCTGCCGTAC
CGGCGCTGGGACTTCGCGGTCGAATTGCATGGGCCGGTAGTAAGCGATATCCGTCAGGCGTTCGAGGTGCAGTGGCGGCG
GATTCGCCTCGGGCATCGGCCGATCGAGTCGATGGTGCCCGATCTCGGGCCGCAGACCGCGGCTTCGCTAGGCAGTCTGC
GGCGCCGGCGCCGCCGCCGTAACGAGGCGCTGTGGGCGGGCGGTCAGCCCTGCGTGGCGTTCGTCGCACGCGACAACCTG
ATCAACCGCCGCGCTATCGAAAAGGCATATCTGGCCGCGATTGGTCAGGCGCGCAATGAGGTGCTGCTCGCCAATCCGTA
CTTCATGCCGGGGCGCAAACTGCGGCGCGCGCTGGTGTTCGCGGCGCGGCGCGGCGTCGTGGTGAAGCTGATCATCGGGC
GCAAGGAATTCAAGGCGCTCGATTATGCTGTGCCGTTTCTGTATCGCTCGCTGCTCAAGGCCGGTGTGCAGATCGCGGAG
TACGAGAAAACGCTGCTGCACGGCAAGGTCGCGGTGGTGGATTCGAACTGGGGGACGGTCGGCTCGTCGAACCTCGACGC
GCTGAGCCTGATGCTCAACAACGAAGCCAACGTAGTGCTGGTGAACGATCCCGCGATCGACGCGCTGCGCGAAGCGATCC
TCGTCGCCTTCAAGGACTCGCGCCGCATCGACGAGGCGCGCTACGACGCCCGTCCGGCCGGCGAGCGCGTGCTCAACTGG
CTCGCTTACACCACCTATCGGGCTGTGATGAAGCTGCTGACGGTCGGCGGCTACGACTGA

Upstream 100 bases:

>100_bases
CATCGCGCTGCTTTTCGTCGTCGAATGCCGATGCGATCCTGCAGTTGCCGAGGCGGTTGAGTGGGCGTGCCGTGGTTGGG
CAATCCGGGGAGCGTAACCG

Downstream 100 bases:

>100_bases
TTCGCGGTGACGCGCTGATGCGTTGGCAAACGCCGGCCGTGCGCGGCCGGTGCGAGTGGAACTGAGGTGCAACGCTTGAG
CCGCGTTGCGCGCCTTGTGC

Product: putative phospholipase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSVGGARRFARLRQSLLGRRYWQRYRFTSGNDVKLLRSGDEFFSALIARVDAAQSDAVLETYIFCHDNAGQAVSAALLRA
AARGVKVRAITDGIGTARLPMFNEWAAAGIDHRIYNPHLFGRFGFSRTHRKLAVIDDQFAFCGGINIVDDYENNGEKLPY
RRWDFAVELHGPVVSDIRQAFEVQWRRIRLGHRPIESMVPDLGPQTAASLGSLRRRRRRRNEALWAGGQPCVAFVARDNL
INRRAIEKAYLAAIGQARNEVLLANPYFMPGRKLRRALVFAARRGVVVKLIIGRKEFKALDYAVPFLYRSLLKAGVQIAE
YEKTLLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVLVNDPAIDALREAILVAFKDSRRIDEARYDARPAGERVLNW
LAYTTYRAVMKLLTVGGYD

Sequences:

>Translated_419_residues
MSVGGARRFARLRQSLLGRRYWQRYRFTSGNDVKLLRSGDEFFSALIARVDAAQSDAVLETYIFCHDNAGQAVSAALLRA
AARGVKVRAITDGIGTARLPMFNEWAAAGIDHRIYNPHLFGRFGFSRTHRKLAVIDDQFAFCGGINIVDDYENNGEKLPY
RRWDFAVELHGPVVSDIRQAFEVQWRRIRLGHRPIESMVPDLGPQTAASLGSLRRRRRRRNEALWAGGQPCVAFVARDNL
INRRAIEKAYLAAIGQARNEVLLANPYFMPGRKLRRALVFAARRGVVVKLIIGRKEFKALDYAVPFLYRSLLKAGVQIAE
YEKTLLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVLVNDPAIDALREAILVAFKDSRRIDEARYDARPAGERVLNW
LAYTTYRAVMKLLTVGGYD
>Mature_418_residues
SVGGARRFARLRQSLLGRRYWQRYRFTSGNDVKLLRSGDEFFSALIARVDAAQSDAVLETYIFCHDNAGQAVSAALLRAA
ARGVKVRAITDGIGTARLPMFNEWAAAGIDHRIYNPHLFGRFGFSRTHRKLAVIDDQFAFCGGINIVDDYENNGEKLPYR
RWDFAVELHGPVVSDIRQAFEVQWRRIRLGHRPIESMVPDLGPQTAASLGSLRRRRRRRNEALWAGGQPCVAFVARDNLI
NRRAIEKAYLAAIGQARNEVLLANPYFMPGRKLRRALVFAARRGVVVKLIIGRKEFKALDYAVPFLYRSLLKAGVQIAEY
EKTLLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVLVNDPAIDALREAILVAFKDSRRIDEARYDARPAGERVLNWL
AYTTYRAVMKLLTVGGYD

Specific function: Catalyzes, in vitro, the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=373, Percent_Identity=40.2144772117962, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1787502, Length=386, Percent_Identity=27.720207253886, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47109; Mature: 46978

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGGARRFARLRQSLLGRRYWQRYRFTSGNDVKLLRSGDEFFSALIARVDAAQSDAVLE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEE
TYIFCHDNAGQAVSAALLRAAARGVKVRAITDGIGTARLPMFNEWAAAGIDHRIYNPHLF
EEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCEE
GRFGFSRTHRKLAVIDDQFAFCGGINIVDDYENNGEKLPYRRWDFAVELHGPVVSDIRQA
EECCCCHHCCEEEEEECCHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECCHHHHHHHHH
FEVQWRRIRLGHRPIESMVPDLGPQTAASLGSLRRRRRRRNEALWAGGQPCVAFVARDNL
HHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHCH
INRRAIEKAYLAAIGQARNEVLLANPYFMPGRKLRRALVFAARRGVVVKLIIGRKEFKAL
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHH
DYAVPFLYRSLLKAGVQIAEYEKTLLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVL
HHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEE
VNDPAIDALREAILVAFKDSRRIDEARYDARPAGERVLNWLAYTTYRAVMKLLTVGGYD
EECCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVGGARRFARLRQSLLGRRYWQRYRFTSGNDVKLLRSGDEFFSALIARVDAAQSDAVLE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEE
TYIFCHDNAGQAVSAALLRAAARGVKVRAITDGIGTARLPMFNEWAAAGIDHRIYNPHLF
EEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCEE
GRFGFSRTHRKLAVIDDQFAFCGGINIVDDYENNGEKLPYRRWDFAVELHGPVVSDIRQA
EECCCCHHCCEEEEEECCHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECCHHHHHHHHH
FEVQWRRIRLGHRPIESMVPDLGPQTAASLGSLRRRRRRRNEALWAGGQPCVAFVARDNL
HHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHCH
INRRAIEKAYLAAIGQARNEVLLANPYFMPGRKLRRALVFAARRGVVVKLIIGRKEFKAL
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHH
DYAVPFLYRSLLKAGVQIAEYEKTLLHGKVAVVDSNWGTVGSSNLDALSLMLNNEANVVL
HHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEE
VNDPAIDALREAILVAFKDSRRIDEARYDARPAGERVLNWLAYTTYRAVMKLLTVGGYD
EECCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]